scholarly journals Exendin-4 effects on islet volume and number in the mouse pancreas

2014 ◽  
Vol 115 (08) ◽  
pp. 502-507
Author(s):  
L. Khorsandi ◽  
F. Nejad-Dehbashi
Keyword(s):  
Mouse Pancreas ◽  
Islet Volume

scholarly journals Exendin-4 effects on islet volume and number in mouse pancreas

2013 ◽  
Vol 49 (4) ◽  
pp. 745-752 ◽  
Author(s):  
Layasadat Khorsandi ◽  
Fereshteh Nejad-Dehbashi
Keyword(s):  
Healthy Adult ◽  
Control Group ◽  
Diabetic Mice ◽  
Mouse Pancreas ◽  
Islet Mass ◽  
Final Injection ◽  
Islet Volume ◽  
Healthy Adult Male ◽  
Pancreas Volume

The aim of this study was to evaluate Exendin-4 (EX-4) effects on islet volume and number in the mouse pancreas. Thirty-two healthy adult male NMRI mice were randomly divided into control and experimental groups. EX-4 was injected intraperitoneally (i. p.) at doses of 0.25 (E1 group), 0.5 (E2 group), and 1 µg/kg (E3 group), twice a day for 7 consecutive days. One day after the final injection, the mice were sacrificed, and the pancreas from each animal dissected out, weighed, and fixed in 10% formalin for measurement of pancreas and islet volume, and determination of islet number by stereological assessments. There was a significant increase in the weight of pancreases in the E3 group. Islet and pancreas volumes in E1 and E2 groups were unchanged compared to the control group. The E3 group showed a significant increase in islet and pancreas volume (P < 0.05). There were no significant changes in the total number of islets in all three experimental groups. The results revealed that EX-4 increased pancreas and islet volume in non-diabetic mice. The increased total islet mass is probably caused by islet hypertrophy without the formation of additional islets.

β-Cell Function Relative to Islet Volume and Hormone Content in the Isolated Perfused Mouse Pancreas*

Endocrinology ◽  
1983 ◽  
Vol 112 (3) ◽  
pp. 1049-1056 ◽  
Author(s):  
V. BONNEVIE-NIELSEN ◽  
LENE THEIL SKOVGAARD ◽  
Å. LERNMARK
Keyword(s):  
Cell Function ◽  
Β Cell ◽  
Mouse Pancreas ◽  
Hormone Content ◽  
Islet Volume ◽  
Β Cell Function

Intraisiet somatostatin inhibits insulin secretion via SSTR5 in the isolated perfused mouse pancreas model

2001 ◽  
Vol 120 (5) ◽  
pp. A133-A133
Author(s):  
T TIRONE ◽  
S MOLDOVAN ◽  
M NORMAL ◽  
F DEMAYO ◽  
F CHARLESBRUNICARDI
Keyword(s):  
Insulin Secretion ◽  
Mouse Pancreas

Identification of MicroRNAs Regulating Ptf1a Expression in Mouse Pancreas Development*

2010 ◽  
Vol 2009 (12) ◽  
pp. 1607-1612
Author(s):  
Yang AN ◽  
Yan-Kun YANG ◽  
Fei GAO ◽  
Kuan-Yu ZHU ◽  
Tan-Wei MU ◽  
...  
Keyword(s):  
Pancreas Development ◽  
Mouse Pancreas

Characterization of the function of transient receptor potential melastatin 2 in mouse pancreas

Pancreatology ◽  
2019 ◽  
Vol 19 ◽  
pp. S94
Author(s):  
Júlia Fanczal ◽  
Petra Pallagi ◽  
Marietta Görög ◽  
Csaba Péter Bíró ◽  
Tamara Madácsy ◽  
...  
Keyword(s):  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Mouse Pancreas ◽  
Transient Receptor Potential Melastatin ◽  
Transient Receptor

scholarly journals Biosynthesis of Ribonuclease in Mouse Pancreas

1960 ◽  
Vol 235 (5) ◽  
pp. 1404-1408
Author(s):  
Allan J. Morris ◽  
Sherman R. Dickman
Keyword(s):  
Mouse Pancreas

Improved Assessment of Isolated Islet Tissue Volume Using Digital Image Analysis

1998 ◽  
Vol 7 (5) ◽  
pp. 469-478 ◽  
Author(s):  
Jan P. Stegemann ◽  
John J. O'Neil ◽  
Don T. Nicholson ◽  
Claudy J.-P. Mullon
Keyword(s):  
Image Analysis ◽  
Digital Image ◽  
Dna Content ◽  
Digital Image Analysis ◽  
Volume Measurement ◽  
Volume Determination ◽  
Tissue Volume ◽  
Porcine Islet ◽  
Islet Volume ◽  
Islet Tissue

Accurate and consistent measurement of tissue volume is critical to performing many types of islet research; however, conventional visual determination of isolated islet yields through a microscope is heavily operator dependent. An improved method of islet volume determination using digital image analysis (DIA) was developed to remove operator bias and automate the islet counting process. A series of 140 porcine islet isolations were used to evaluate the DIA method in three separate stages. In Stage 1 ( n = 29 isolations), the conventional and DIA methods were correlated with two other independent islet quantitation methods: insulin extraction, and DNA extraction. It was found that volumes determined by DIA correlated more closely with insulin content and DNA content than did conventionally determined volumes. In Stages 2 and 3 ( n = 54 and 57 isolations, respectively), it was shown that an increase in the number of fields analyzed by DIA did not significantly improve the quality of the correlations. Inclusion of very small tissue (<50 fun in diameter), which is ignored in the conventional protocol affected yields by less than 10% and did not significantly improve the correlation with insulin or DNA content. Quantitation of isolated islet tissue volume using DIA has been shown to be rapid, consistent, and objective. In the laboratory, use of this method as the standard for islet volume measurement will allow more meaningful comparison of experimental results between centers. In the clinic, its use will allow more accurate dosing of transplanted tissue. © 1998 Elsevier Science Inc.

scholarly journals NUCLEIC ACID PHOSPHORUS OF MOUSE PANCREAS AFTER PILOCARPINE ADMINISTRATION

1952 ◽  
Vol 198 (2) ◽  
pp. 815-820 ◽  
Author(s):  
M. Rabinovitch ◽  
V. Valeri ◽  
H.A. Rothschild ◽  
S. Camara ◽  
A. Sesso ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Mouse Pancreas
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