scholarly journals α2,3- and α2,6-linked sialic acids are important for cell binding and replication of Newcastle disease virus in chicken primary neuronal cells

2018 ◽  
Vol 62 (03) ◽  
pp. 235-245 ◽  
Author(s):  
Y. GUO ◽  
J. ZHAO ◽  
X. CHANG ◽  
W. YAO ◽  
H. WANG ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Neuronal Cells ◽  
Sialic Acids ◽  
Cell Binding ◽  
Primary Neuronal Cells

scholarly journals Host CARD11 Inhibits Newcastle Disease Virus Replication by Suppressing Viral Polymerase Activity in Neurons

2019 ◽  
Vol 93 (24) ◽  
Author(s):  
Wenbin Wang ◽  
Xudong Chang ◽  
Wei Yao ◽  
Ning Wei ◽  
Na Huo ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Viral Replication ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Polymerase Activity ◽  
Viral Polymerase ◽  
Cytopathic Effects ◽  
Primary Neuronal Cells

ABSTRACT Host factors play multiple essential roles in the replication and pathogenesis of mammalian neurotropic viruses. However, the cellular proteins of the central nervous system (CNS) involved in avian neurotropic virus infection have not been completely elucidated. Here, we employed a gene microarray to identify caspase recruitment domain-containing protein 11 (CARD11), a lymphoma-associated scaffold protein presenting brain-specific upregulated expression in a virulent neurotropic Newcastle disease virus (NDV)-infected natural host. Chicken primary neuronal cells infected with NDV appeared slightly syncytial and died quickly. CARD11 overexpression inhibited viral replication and delayed cytopathic effects; conversely, depletion of CARD11 enhanced viral replication and cytopathic effects in chicken primary neuronal cells. The inhibition of viral replication by CARD11 could not be blocked with CARD11-Bcl10-MALT1 (CBM) signalosome and NF-κB signaling inhibitors. CARD11 was found to interact directly with the viral phosphoprotein (P) through its CC1 domain and the X domain of P; this X domain also mediated the interaction between P and the viral large polymerase protein (L). The CARD11 CC1 domain and L competitively bound to P via the X domain that hindered the P-L interaction of the viral ribonucleoprotein (RNP) complex, resulting in a reduction of viral polymerase activity in a minigenome assay and inhibition of viral replication. Animal experiments further revealed that CARD11 contributed to viral replication inhibition and neuropathology in infected chicken brains. Taken together, our findings identify CARD11 as a brain-specific antiviral factor of NDV infection in avian species. IMPORTANCE Newcastle disease virus (NDV) substantially impacts the poultry industry worldwide and causes viral encephalitis and neurological disorders leading to brain damage, paralysis, and death. The mechanism of interaction between this neurotropic virus and the avian central nervous system (CNS) is largely unknown. Here, we report that host protein CARD11 presented brain-specific upregulated expression that inhibited NDV replication, which was not due to CARD11-Bcl10-MALT1 (CBM) complex-triggered activation of its downstream signaling pathways. The inhibitory mechanism of viral replication is through the CARD11 CC1 domain, and the viral large polymerase protein (L) competitively interacts with the X domain of the viral phosphoprotein (P), which hampers the P-L interaction, suppressing the viral polymerase activity and viral replication. An in vivo study indicated that CARD11 alleviated neuropathological lesions and reduced viral replication in chicken brains. These results provide insight into the interaction between NDV infection and the host defense in the CNS and a potential antiviral target for viral neural diseases.

scholarly journals α2-3- and α2-6- N-linked sialic acids allow efficient interaction of Newcastle Disease Virus with target cells

2012 ◽  
Vol 29 (7) ◽  
pp. 539-549 ◽  
Author(s):  
Lorena Sánchez-Felipe ◽  
Enrique Villar ◽  
Isabel Muñoz-Barroso
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Sialic Acids ◽  
Target Cells

Characterization of Recent Isolates of Newcastle Disease virus

2016 ◽  
Vol 21 (1) ◽  
pp. 235-248
Author(s):  
Amani Saleh ◽  
Rola Ali ◽  
Mohamed Fawzy ◽  
Mokhtar Eltarabily
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease

Transcription and translation of Newcastle disease virus mRNA's in vitro.

1978 ◽  
Vol 28 (1) ◽  
pp. 324-336 ◽  
Author(s):  
P L Collins ◽  
L E Hightower ◽  
L A Ball
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease
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