scholarly journals Transmissible gastroenteritis virus nsp7 protein localized in the cytoplasm down-regulates interleukin 8 expression in porcine intestinal epithelial cell

2018 ◽  
Vol 62 (01) ◽  
pp. 41-49
Author(s):  
Q. ZHANG ◽  
J. L. HUANG ◽  
Y. B. LIANG ◽  
Y. P. HE ◽  
D. W. TONG ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Interleukin 8 ◽  
Transmissible Gastroenteritis Virus ◽  
Intestinal Epithelial ◽  
Gastroenteritis Virus ◽  
Porcine Intestinal Epithelial Cell ◽  
Transmissible Gastroenteritis

scholarly journals Identification and analysis of long non-coding RNAs that are involved in inflammatory process in response to transmissible gastroenteritis virus infection

2019 ◽  
Author(s):  
Xuelian Ma ◽  
Xiaomin Zhao ◽  
Kaili Wang ◽  
Xiaoyi Tang ◽  
Jianxiong Guo ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Messenger Rna ◽  
Binding Proteins ◽  
Differentially Expressed ◽  
Transmissible Gastroenteritis Virus ◽  
Intestinal Epithelial ◽  
Antisense Gene ◽  
Gastroenteritis Virus ◽  
Expression Of Genes ◽  
Transmissible Gastroenteritis

Abstract Abstract Background: Transmissible gastroenteritis virus (TGEV) infection can cause acute inflammation. Long noncoding RNAs (lncRNAs) play important roles in a number of biological process including inflammation response. However, whether lncRNAs participate in TGEV-induced inflammation in porcine intestinal epithelial cells (IPECs) is largely unknown. Results: In this study, the next-generation sequencing (NGS) technology was used to analyze the profiles of lncRNAs in Mock and TGEV-infected porcine intestinal epithelial cell-jejunum 2 (IPEC-J2) cell line. A total of 106 lncRNAs were differentially expressed. Many differentially expressed lncRNAs act as elements to competitively attach microRNAs (miRNAs) which target to messenger RNA (mRNAs ) to mediate expression of genes that related to Toll-like receptors (TLRs), NOD-like receptors (NLRs), Tumor necrosis factor (TNF), and RIG-I-like receptor s (RLRs) pathways. Functional analysis of the binding proteins and the up/down-stream genes of the differentially expressed lncRNAs revealed that lncRNAs were principally related to inflammatory response. Meanwhile, we found that the differentially expressed lncRNA TCONS_00058367 might lead to a reduction of phosphorylation of transcription factor p65 (p-p65) in TGEV-infected IPEC-J2 cells by negatively regulating its antisense gene romyelocytic leukemia (PML ). Conclusions: The data showed that differentially expressed lncRNAs might be involved in inflammatory response induced by TGEV through acting as miRNA sponges, regulating their up/down-stream genes, or directly binding proteins.

Characterization of a porcine intestinal epithelial cell line for in vitro studies of microbial pathogenesis in swine

2005 ◽  
Vol 125 (3) ◽  
pp. 293-305 ◽  
Author(s):  
Peter Schierack ◽  
Marcel Nordhoff ◽  
Marion Pollmann ◽  
Karl Dietrich Weyrauch ◽  
Salah Amasheh ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Intestinal Epithelial Cell ◽  
In Vitro Studies ◽  
Epithelial Cell Line ◽  
Intestinal Epithelial ◽  
Microbial Pathogenesis ◽  
Porcine Intestinal Epithelial Cell

scholarly journals Identification and analysis of long non-coding RNAs that are involved in inflammatory process in response to transmissible gastroenteritis virus infection

2019 ◽  
Author(s):  
Xuelian Ma ◽  
Xiaomin Zhao ◽  
Kaili Wang ◽  
Xiaoyi Tang ◽  
Jianxiong Guo ◽  
...  
Keyword(s):  
Immune Response ◽  
Binding Proteins ◽  
Differentially Expressed ◽  
Transmissible Gastroenteritis Virus ◽  
Intestinal Epithelial ◽  
Antisense Gene ◽  
Gastroenteritis Virus ◽  
Expression Of Genes ◽  
Transmissible Gastroenteritis ◽  
Mirna Sponges

Abstract Abstract Background: Transmissible gastroenteritis virus (TGEV) infection can activate the immune response and cause inflammation. Long noncoding RNAs (lncRNAs) play important roles in antiviral innate immune response. However, whether lncRNAs participate in TGEV-induced inflammation in porcine intestinal epithelial cells (IPECs) is largely unknown. Results: In this study, the next-generation sequencing (NGS) technology was used to analyze the profiles of lncRNAs in Mock and TGEV-infected porcine intestinal epithelial cell-jejunum 2 (IPEC-J2) cell line. A total of 106 lncRNAs were differentially expressed. Many differentially expressed lncRNAs act as elements to competitively attach miRNAs with mRNAs to mediate expression of genes that related to Toll-like receptor, NOD-like receptor, TNF, and RIG-I-like receptor pathways. Functional analysis of the binding proteins and the up/down-stream genes of the differentially expressed lncRNAs revealed that lncRNAs were principally related to immune response. Meanwhile, we found that the differentially expressed lncRNA TCONS_00058367 might lead to a reduction of p-p65 in TGEV-infected IPEC-J2 cells by negatively regulating its antisense gene PML. Conclusions: The data showed that differentially expressed lncRNAs might be involved in immune response induced by TGEV through acting as miRNA sponges, regulating their up/down-stream genes, or directly binding proteins.

scholarly journals Gentamicin sulphate permeation through porcine intestinal epithelial cell monolayer

2015 ◽  
Vol 63 (1) ◽  
pp. 60-68
Author(s):  
Béla Gyetvai ◽  
Ákos Jerzsele ◽  
Erzsébet Pászti-Gere ◽  
Gábor Nagy ◽  
Péter Gálfi
Keyword(s):  
Epithelial Cell ◽  
High Performance ◽  
Intestinal Epithelial Cell ◽  
Cell Monolayer ◽  
Aminoglycoside Antibiotic ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Epithelial Cell Line ◽  
Intestinal Epithelial ◽  
Porcine Intestinal Epithelial Cell

Gentamicin is an aminoglycoside antibiotic widely used in combination with dimethyl sulphoxide (DMSO) in topical drug formulations. It is not known, however, whether DMSO can enhance the permeation of gentamicin through biological membranes, leading to oto- and nephrotoxic side effects. A simple and reliable high-performance liquid chromatographic (HPLC) method was applied for the quantitative determination of gentamicin collected from the apical and basolateral compartments of the porcine intestinal epithelial cell line IPEC-J2 cell monolayer using fluorometric derivatisation of the analyte with fluorenylmethyloxycarbonyl chloride (FMOC) prior to chromatographic run in the presence and absence of 1% DMSO. The lack of change in transepithelial electrical resistance (TER) demonstrated that gentamicin and 1% DMSO did not affect IPEC-J2 cell monolayer integrity via the disruption of cell membranes. Chromatographic data also ascertained that gentamicin penetration across the cell monolayer even in the presence of 1% DMSO was negligible at 6 h after the beginning of apical gentamicin administration. This study further indicates that the addition of this organic solvent does not increase the incidence of toxic effects related to gentamicin permeation.

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