scholarly journals Calcium signaling involved in bovine herpesvirus 1 replication in MDBK cells

2017 ◽  
Vol 61 (04) ◽  
pp. 487-491 ◽  
Author(s):  
L. ZHU ◽  
L. HUANG ◽  
Y. ZHU ◽  
X. DING ◽  
G. ZHU
Keyword(s):  
Calcium Signaling ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Mdbk Cells ◽  
Herpesvirus 1

scholarly journals Origin Of Cytoplasmic Nucleocapsids In Mdbk-Cells Infected With Bovine Herpesvirus 1 (Bhv-1)

2002 ◽  
Vol 8 (S02) ◽  
pp. 986-987
Author(s):  
P. Wild ◽  
E.M. Schraner ◽  
E. Loepfe ◽  
P. Walther ◽  
M. Müller ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Mdbk Cells ◽  
Herpesvirus 1

Modulation of telomerase activity, bTERT and c-Myc induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin during Bovine Herpesvirus 1 infection in MDBK cells

2014 ◽  
Vol 28 (1) ◽  
pp. 24-30 ◽  
Author(s):  
Filomena Fiorito ◽  
Antonietta Cantiello ◽  
Giovanna Elvira Granato ◽  
Gabriella Marfè ◽  
Roberto Ciarcia ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Telomerase Activity ◽  
Bovine Herpesvirus 1 ◽  
Mdbk Cells ◽  
Herpesvirus 1 ◽  
Tetrachlorodibenzo P Dioxin

scholarly journals Impairment of Nuclear Pores in Bovine Herpesvirus 1-Infected MDBK Cells

2005 ◽  
Vol 79 (2) ◽  
pp. 1071-1083 ◽  
Author(s):  
Peter Wild ◽  
Monika Engels ◽  
Claudia Senn ◽  
Kurt Tobler ◽  
Urs Ziegler ◽  
...  
Keyword(s):  
High Resolution ◽  
Nuclear Membrane ◽  
Bovine Herpesvirus ◽  
Nuclear Surface ◽  
Nuclear Pores ◽  
Bovine Herpesvirus 1 ◽  
Golgi Membranes ◽  
Perinuclear Space ◽  
Mdbk Cells ◽  
Herpesvirus 1

ABSTRACT Herpesvirus capsids originating in the nucleus overcome the nucleocytoplasmic barrier by budding at the inner nuclear membrane. The fate of the resulting virions is still under debate. The fact that capsids approach Golgi membranes from the cytoplasmic side led to the theory of fusion between the viral envelope and the outer nuclear membrane, resulting in the release of capsids into the cytoplasm. We recently discovered a continuum from the perinuclear space to the Golgi complex implying (i) intracisternal viral transportation from the perinuclear space directly into Golgi cisternae and (ii) the existence of an alternative pathway of capsids from the nucleus to the cytoplasm. Here, we analyzed the nuclear surface by high-resolution microscopy. Confocal microscopy of MDBK cells infected with recombinant bovine herpesvirus 1 expressing green fluorescent protein fused to VP26 (a minor capsid protein) revealed distortions of the nuclear surface in the course of viral multiplication. High-resolution scanning and transmission electron microscopy proved the distortions to be related to enlargement of nuclear pores through which nuclear content including capsids protrudes into the cytoplasm, suggesting that capsids use impaired nuclear pores as gateways to gain access to the cytoplasmic matrix. Close examination of Golgi membranes, rough endoplasmic reticulum, and outer nuclear membrane yielded capsid-membrane interaction of high identity to the budding process at the inner nuclear membrane. These observations signify the ability of capsids to induce budding at any cell membrane, provided the fusion machinery is present and/or budding is not suppressed by viral proteins.

153 EFFECTS OF OVINE INTERFERON-β ON REPLICATION OF BOVINE VIRAL DIARRHEA VIRUS AND BOVINE HERPESVIRUS-1

2008 ◽  
Vol 20 (1) ◽  
pp. 156 ◽  
Author(s):  
P. K. Galik ◽  
J. A. Gard ◽  
T. S. Spencer ◽  
M. S. Marley ◽  
D. A. Stringfellow ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Bovine Herpesvirus ◽  
Bovine Viral Diarrhea ◽  
Bovine Herpesvirus 1 ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Mdbk Cells ◽  
Herpesvirus 1 ◽  
Viral Diarrhea Virus

Bovine viral diarrhea virus (BVDV) and bovine herpesvirus-1 (BHV-1) are the most commonly isolated viruses from abattoir-origin materials utilized in embryo production and known to associate with zona pellucida-intact (ZP-I) embryos after exposure and washing. Some evidence indicates that developing embryos may produce substances that are able to inhibit viral replication in adjacent cells. Interferons such as recombinant human interferon-α are known to have anti-BVDV activity but no effect against BHV-1. In some preliminary studies, bovine interferon-τ has shown antiviral activities against BVDV but not against BHV-1. However, interferon-τ in other species has not been evaluated for anti-BVDV and anti-BHV-1 effects. Thus, the objective of this study was to evaluate the cytotoxicity and anti-viral effect of ovine interferon-τ against a non-cytopathic high affinity strain of BVDV (SD-1) and BHV-1 (Colorado) in cell culture. Serial dilutions (1:10) beginning with an initial concentration of 1 mg mL–1 of interferon-τ were made in 96-well plates and then Madin Darby bovine kidney (MDBK) cells were seeded in the wells. Cells and interferon-τ were incubated at 37.5�C in 5% CO2 and air for 24 h prior to addition of virus. The following concentrations of BVDV were added to the wells: 6000, 3500, 1000, 625, and 350 cell culture infective doses (CCID50) (50% endpoint) per well. In addition, four viral concentrations of BHV-1, 1000, 500, 250, and 100 CCID50/mL were evaluated in separate cell cultures. Virus isolation was utilized to determine if the addition of interferon-τ decreased the amount of infective virus. Ovine interferon-τ produced no observable cytotoxicity in MDBK cells in any of the assays. Also, the three highest concentrations of interferon-τ significantly decreased the amount of BVDV in all of the concentrations of BVDV tested but had no apparent effect on the concentration of BHV-1 in cell cultures. Therefore ovine interferon-τ has anti-BVDV effects similar to those seen with bovine interferont-τ and neither has any apparent antiviral activity on BHV-1 in cell culture. Additionally, ovine and bovine interferon-τ might serve to limit or prevent the transmission of BVDV and curtail the negative effects of BVDV on oocyte and embryo development. However, a similar effect is not expected for BHV-1.

scholarly journals Bovine Herpesvirus 1 Entry by a Low-pH Endosomal Pathway

2018 ◽  
Vol 92 (20) ◽  
Author(s):  
Gabrielle Pastenkos ◽  
Becky Lee ◽  
Suzanne M. Pritchard ◽  
Anthony V. Nicola
Keyword(s):  
Bovine Herpesvirus ◽  
Low Ph ◽  
Dependent Manner ◽  
Bovine Herpesvirus 1 ◽  
African Green Monkey Kidney ◽  
Effective Prevention ◽  
Entry Pathway ◽  
Lysosomotropic Agents ◽  
Mdbk Cells ◽  
Herpesvirus 1

ABSTRACTBovine herpesvirus 1 (BoHV-1) is an alphaherpesvirus that poses a significant challenge to health and welfare in the cattle industry. We investigated the cellular entry route utilized by BoHV-1. We report that BoHV-1 enters Madin Darby bovine kidney (MDBK) cells, bovine turbinate cells, and African green monkey kidney (Vero) cells via a low-pH-mediated endocytosis pathway. Treatment of MDBK cells with hypertonic medium, which inhibits receptor-mediated endocytosis, prevented infection as measured by a beta-galactosidase reporter assay. Treatment of cells with noncytotoxic concentrations of the lysosomotropic agents ammonium chloride and monensin, which block the acidification of endosomes, inhibited BoHV-1 entry in a concentration-dependent fashion. The kinetics of endocytic uptake of BoHV-1 from the cell surface was rapid (50% uptake by ∼5 min). Time-of-addition experiments indicated that the lysosomotropic agents acted at early times postinfection, consistent with entry. Inactivation of virions by pretreatment with mildly acidic pH is a hallmark characteristic of viruses that utilize a low-pH-activated entry pathway. When BoHV-1 particles were exposed to pH 5.0 in the absence of target membrane, infectivity was markedly reduced. Lastly, treatment of cells with the proteasome inhibitor MG132 inhibited BoHV-1 entry in a concentration-dependent manner. Together, these results support a model of BoHV-1 infection in which low endosomal pH is a critical host trigger for fusion of the viral envelope with an endocytic membrane and necessary for successful infection of the target cell.IMPORTANCEBoHV-1 is a ubiquitous pathogen affecting cattle populations worldwide. Infection can result in complicated, polymicrobial infections due to the immunosuppressive properties of the virus. While there are vaccines on the market, they only limit disease severity and spread but do not prevent infection. The financial and animal welfare ramifications of this virus are significant, and in order to develop more effective prevention and treatment regimens, a more complete understanding of the initial steps in viral infection is necessary. This research establishes the initial entry pathway of BoHV-1, which provides a foundation for future development of effective treatments and preventative vaccines. Additionally, it allows comparisons to the entry pathways of other alphaherpesviruses, such as HSV-1.

scholarly journals Investigations of presence of antibodies against bovine herpesvirus-1 in blood serum of calves prior to colostrum diet

2010 ◽  
Vol 64 (1-2) ◽  
pp. 33-41
Author(s):  
Sava Lazic ◽  
Dragan Rogan ◽  
Tamas Petrovic ◽  
Dejan Bugarski ◽  
Diana Lupulovic ◽  
...  
Keyword(s):  
Blood Serum ◽  
Antibody Titer ◽  
Bovine Herpesvirus ◽  
Virus Neutralization ◽  
Serum Samples ◽  
Bovine Herpesvirus 1 ◽  
Breeding Material ◽  
Mdbk Cells ◽  
Herpesvirus 1

The paper presents the results of investigations of the presence of the bovine herpesvirus-1 (BHV-1) in samples of blood serum from 106 cows and 107 of their calves (one cow had twins). Blood was sampled from the cows immediately after parturition, and from the calves before feeding on colostrum. The examined cows and their calves originated from 5 herds in which previous investigations had shown infection with the bovine herpesvirus-1. The determination of antibodies against BHV-1 was performed using the method of virus neutralization in culture of MDBK cells with 100 TCID/50 viruses (BHV-1, TN-41 Am. Bio Research, USA). Antibodies against BHV-1 were determined in all blood serum samples of cows and in 16 samples of precolostral blood serums of calves. The antibody titer values in cows ranged from 1:4 to 1:512, and in calves the determined values were from 1:2 to 1:16. The results indicate that cows that are seropositive to BHV-1 can deliver calves seropositive to BHV-1 in about 15% cases. This must be kept in mind in selecting cows for the production of breeding material, in particular bulls for reproduction centers, as well as in making a programme for the immunoprophylaxis of calves against BHV-1. .

scholarly journals In vitro interaction of bovine herpesvirus 1 with uterine tube epithelial cells and oocytes

2016 ◽  
Vol 82 (0) ◽  
Author(s):  
Roseli Fernandes Gonçalves ◽  
Rodrigo Martins Soares ◽  
Cássia Maria Barroso Orlandi ◽  
Leonardo José Richtzenhain ◽  
José Antonio Visintin ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cytopathic Effect ◽  
Bovine Herpesvirus ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Bovine Herpesvirus 1 ◽  
Mdbk Cells ◽  
Herpesvirus 1 ◽  
Polymerase Chain

The aims of this study were to assess in vitro if bovine oocytes and oviductal epithelial cells from slaughterhouses for in vitro fertilization use may be infected with bovine herpesvirus 1; to analyze whether the treatment with trypsin according to the International Embryo Transfer Society guideline is efficient to inactivate the bovine herpesvirus 1; to morphologically study the virus-oocyte interaction through optical microscopy. In this study, Madin Darby Bovine Kidney (MDBK) cells that were co-cultured with oocytes matured in vitro and exposed to bovine herpesvirus 1 showed a cytopathic effect. The nested polymerase chain reaction for the supernatant was positive for the bovine herpesvirus 1, thus suggesting that the cytopathic effect observed in the MDBK monolayer was seen due to virus replication and not because of any culture toxicity. It was also observed cytopathic effect and positive nested polymerase chain reaction in MDBK cells co-cultured with in vitro maturated oocytes free of virus, but that were co-cultured in uterine epithelial cells pre-infected with bovine herpesvirus 1 and washed or not with trypsin, demonstrating an oocyte contamination by the virus. When trypsin-washing efficacy was evaluated, we could observe that the trypsin treatment was not able to eliminate the bovine herpesvirus 1 of the oocytes, and it was not observed any morphological difference in the infected oocytes.

Study of programmed cell death in bovine herpesvirus 1 infected mdbk cells and the possible role of nitric oxide in this process

2004 ◽  
Vol 52 (3) ◽  
pp. 287-297 ◽  
Author(s):  
Zafer Yazici ◽  
Yasemin Baskin ◽  
H. Baskin ◽  
Ozlem Gecer ◽  
I. Hakki Bahar ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Bacterial Infections ◽  
Caspase 3 ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Mdbk Cells ◽  
Herpesvirus 1 ◽  
Induced Apoptosis

Bovine herpesvirus 1 (BHV-1) is the aetiological agent of many disease types and may predispose infected animals, possibly through immunosuppression, to secondary bacterial infections. Immunosuppression may directly be associated with the induction of programmed cell death (PCD) in some virus-infected cells. Nitric oxide (NO) has an important mediating role against fungal, bacterial, protozoal, viral pathogens and tumours. BHV-1 induced apoptosis between 0.5-3 h postinfection (PI) in MDBK cells; however, between 3 and 6 h PI the PCD response was found to be decreased. It was interesting to see that BHV-1 inhibited staurosporin-induced PCD after 1 h. These results showed similarities with those obtained from herpes simplex type 1 infections in human epithelial cells. PCD response decreased 1 h following caspase-3 inhibitor applications, whereas NO response increased 3 h following infection in the presence of caspase-8 and -9 inhibitory peptides. In conclusion, BHV-1 inhibited the staurosporin-induced apoptotic response and also the NO response. We propose that this inhibition is caspase-3 dependent.

210 EFFECTS OF INTERFERON-τ ON REPLICATION OF BOVINE VIRAL DIARRHEA VIRUS AND BOVINE HERPESVIRUS-1

2006 ◽  
Vol 18 (2) ◽  
pp. 213 ◽  
Author(s):  
P. Galik ◽  
J. Waldrop ◽  
S. Marley
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Bovine Herpesvirus ◽  
Bovine Viral Diarrhea ◽  
Bovine Herpesvirus 1 ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Mdbk Cells ◽  
Herpesvirus 1 ◽  
Viral Diarrhea Virus

Bovine viral diarrhea virus (BVDV) and bovine herpesvirus-1 (BHV-1) are the most likely viruses to be associated with abattoir-origin materials used in in vitro embryo production. Further, both viruses are known to associate with zona pellucida-intact embryos after exposure and washing, and limited evidence indicates that developing, transferable embryos are able to inhibit viral replication in adjacent cells. Interferon-τ is known to have anti-BVDV and anti-BHV-1 activities, but it is not known whether interferon-τ which is secreted by developing embryos has the same effects. The objectives of this study were to evaluate the cytotoxicity and anti-viral effect of interferon-τ against a non-cytotoxic high affinity strain of BVDV (SD-1) and against a strain of BHV-1 (Colorado) in cell culture. Madin Darby bovine kidney (MDBK) cells were seeded in 96-well plates and then inoculated with serial dilutions (1:10) beginning with an initial concentration of 0.2 �g of interferon-τ. Cells and interferon were incubated at 37.5�C in 5% CO2 and air for 24 h prior to addition of virus. Five concentrations of BVDV were added to the wells to give 500, 50, 25, 10 or 5 cell culture infective doses (50% endpoint) per well. Three concentrations of BHV-1-50, 10, and 5 plaque-forming units were evaluated in separate cell cultures. Virus isolation (for BVDV) or plaque assays (for BHV-1) were utilized to determine if the addition of interferon-τ decreased the amount of infective virus. The interferon-τ produced no observable cytotoxicity in MDBK cells in any of the assays. At its three highest concentrations, the interferon-τ significantly decreased the amount of BVDV but it had no significant effect on the amount of BHV-1 in cell cultures. Thus, it is possible that interferon-τ produced by developing embryos might limit or prevent transmission of BVDV to recipients if this virus were to be inadvertently associated with the embryos that are transferred. However, a similar effect is not expected for BHV-1.

Production of Bovine Herpesvirus-1 Vaccine Strains in MDBK Cells Using BelloCell Bioreactor

2016 ◽  
Vol 12 (1) ◽  
pp. 1-7
Author(s):  
Lekshmi Rajan ◽  
Aman Kamboj ◽  
Bikash Prusty ◽  
Tapan Palai ◽  
L Ananthakrishna ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Mdbk Cells ◽  
Herpesvirus 1
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