scholarly journals Immunodetection and N-terminal sequencing of DNA replication proteins of bacteriophage BFK20 – lytic phage of Brevibacterium flavum

2014 ◽  
Vol 58 (02) ◽  
pp. 152-159 ◽  
Author(s):  
G. BUKOVSKÁ ◽  
N. HALGAŠOVÁ ◽  
L. HROMADOVÁ ◽  
H. KOŠČOVÁ ◽  
M. BUKOVSKÝ
Keyword(s):  
Dna Replication ◽  
Lytic Phage ◽  
Replication Proteins ◽  
Brevibacterium Flavum

Overlapping Roles of the Spindle Assembly and DNA Damage Checkpoints in the Cell-Cycle Response to Altered Chromosomes in Saccharomyces cerevisiae

Genetics ◽  
2002 ◽  
Vol 161 (2) ◽  
pp. 521-534
Author(s):  
Peter M Garber ◽  
Jasper Rine
Keyword(s):  
Dna Damage ◽  
Dna Replication ◽  
Spindle Checkpoint ◽  
Dna Lesions ◽  
Replication Proteins ◽  
Replication Checkpoint ◽  
Dna Damage Checkpoints ◽  
Dna Replication Checkpoint ◽  
Mcm Proteins ◽  
Stalled Replication Forks

Abstract The MAD2-dependent spindle checkpoint blocks anaphase until all chromosomes have achieved successful bipolar attachment to the mitotic spindle. The DNA damage and DNA replication checkpoints block anaphase in response to DNA lesions that may include single-stranded DNA and stalled replication forks. Many of the same conditions that activate the DNA damage and DNA replication checkpoints also activated the spindle checkpoint. The mad2Δ mutation partially relieved the arrest responses of cells to mutations affecting the replication proteins Mcm3p and Pol1p. Thus a previously unrecognized aspect of spindle checkpoint function may be to protect cells from defects in DNA replication. Furthermore, in cells lacking either the DNA damage or the DNA replication checkpoints, the spindle checkpoint contributed to the arrest responses of cells to the DNA-damaging agent methyl methanesulfonate, the replication inhibitor hydroxyurea, and mutations affecting Mcm2p and Orc2p. Thus the spindle checkpoint was sensitive to a wider range of chromosomal perturbations than previously recognized. Finally, the DNA replication checkpoint did not contribute to the arrests of cells in response to mutations affecting ORC, Mcm proteins, or DNA polymerase δ. Thus the specificity of this checkpoint may be more limited than previously recognized.

scholarly journals The Revisited Genome of Bacillus subtilis Bacteriophage SPP1

Viruses ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 705 ◽  
Author(s):  
Lia M. Godinho ◽  
Mehdi El Sadek Fadel ◽  
Céline Monniot ◽  
Lina Jakutyte ◽  
Isabelle Auzat ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Dna Replication ◽  
Molecular Mechanisms ◽  
Replication Proteins ◽  
Particle Assembly ◽  
Multiplication Process ◽  
Viral Particles ◽  
Late Transcription ◽  
Transcriptional Units ◽  
Subtilis Bacteriophage

Bacillus subtilis bacteriophage SPP1 is a lytic siphovirus first described 50 years ago [1]. Its complete DNA sequence was reported in 1997 [2]. Here we present an updated annotation of the 44,016 bp SPP1 genome and its correlation to different steps of the viral multiplication process. Five early polycistronic transcriptional units encode phage DNA replication proteins and lysis functions together with less characterized, mostly non-essential, functions. Late transcription drives synthesis of proteins necessary for SPP1 viral particles assembly and for cell lysis, together with a short set of proteins of unknown function. The extensive genetic, biochemical and structural biology studies on the molecular mechanisms of SPP1 DNA replication and phage particle assembly rendered it a model system for tailed phages research. We propose SPP1 as the reference species for a new SPP1-like viruses genus of the Siphoviridae family.

Synthesis and decay of λ DNA replication proteins in minicells

1980 ◽  
Vol 92 (1) ◽  
pp. 120-126 ◽  
Author(s):  
Barbara Lipinska ◽  
Anna Podhajska ◽  
Karol Taylor
Keyword(s):  
Dna Replication ◽  
Replication Proteins
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