Molecular analysis of the coat protein gene of peanut stripe virus from China

2011 ◽  
Vol 55 (2) ◽  
pp. 123-129
Author(s):  
S. HOU ◽  
Y. CHI ◽  
Y. LIU ◽  
X. LI ◽  
S. YU ◽  
...  
Keyword(s):  
Coat Protein ◽  
Molecular Analysis ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Peanut Stripe Virus

Strains of Turnip mosaic potyvirus as defined by the molecular analysis of the coat protein gene of the virus

2003 ◽  
Vol 94 (1) ◽  
pp. 33-43 ◽  
Author(s):  
F Sánchez ◽  
X Wang ◽  
C.E Jenner ◽  
J.A Walsh ◽  
F Ponz
Keyword(s):  
Coat Protein ◽  
Molecular Analysis ◽  
Coat Protein Gene ◽  
Protein Gene

scholarly journals PENGKLONAN DAN PERUNUTAN NUKLEOTIDA GEN SELUBUNG PROTEIN DAN 3’UTR (untranslated region) PEANUT STRIPE VIRUS

2001 ◽  
Vol 1 (1) ◽  
pp. 1-6
Author(s):  
Hasriadi Mat Akin
Keyword(s):  
Amino Acid ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Cleavage Site ◽  
Untranslated Region ◽  
Protein Gene ◽  
Start Codon ◽  
Genomic Rna ◽  
Cp Gene ◽  
Peanut Stripe Virus

Cloning and sequencing of coat protein gene and 3’UTR (untranslated region) of peanut stripe virus. The cDNA of 3' terminal of peanut stripe virus genomic RNA was cloned and sequenced. The cDNA was ligated with plasmid vector pGEM-T Easy and transformed to competent cells of Escherichia coli. The 3' terminal of PstV genomic RNA contained 1195 nucleotides (nts).  The region included the nucleotide sequences of NIb (nuclear inclusion body) (129 nts), CP gene (coat protein gene) (861 nts), and 3'UTR (untranslated region) (205 nts). The nucleotide sequence of a CP gene contained one long uninterrupted open reading frame (ORF) without a start codon, which ended a UAG stop codon. The 287 amino acid residues of PStV coat protein were predicted from the CP gene.  The amino acid was analyzed for the presence of consensus polyprotein cleavage site for maturation of potyvirus polyprotein.  A putative cleavage site was found at position 43 (Q/S) following the Valine (V) residue at -4 position.  This isolate of PstV can be expected to be aphid transmissible because the coat protein contained a DAG triplet at position 53-55.

scholarly journals TRANSGENE IDENTITY AND NUMBER OF INTEGRATION SITES AND THEIR CORRELATION WITH RESISTANCE TO PStV IN TRANSGENIC PEANUTS CARRYING PEANUT STRIPE VIRUS (PStV) COAT PROTEIN GENE

2007 ◽  
Vol 7 (1) ◽  
pp. 39-47
Author(s):  
Dwi Hapsoro ◽  
Hajrial Aswidinnoor ◽  
Rusmilah Suseno ◽  
Jumanto Jumanto ◽  
Sudarsono Sudarsono
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Copy Number ◽  
Protein Gene ◽  
Insertion Site ◽  
Southern Analysis ◽  
Cp Gene ◽  
Peanut Stripe Virus ◽  
Integration Sites ◽  
Transgenic Peanut

Transgene Identity and Number of Integration Sites and Their Correlation with Resistance To PStV in Transgenic Peanuts Carrying Peanut Stripe Virus (PStV) Coat Protein Gene. This research aimed to determine (1) the identity and copy number of PStV cp gene in transgenic peanut plants carrying PStV cp gene and (2) correlation between the identity and the number of integration sites and resistance to PStV infection.  One T0 transgenic peanut was selfed up to five generations. T2, T3, and T5 plants were mechanically inoculated with PStV. Samples of T5 plants derived from several different T4 plants were subjected to Southern analysis to confirm the integration of PStV cp gene and to determine its identity and copy number. The Southern analysis showed three bands of different size, i.e. 1.1 kb, 1.3 kb, and 5.8 kb. Most of the lines of T5 generation have one insertion site, suggesting that the three insertion sites were located in different loci. Based on the phenotypic data, the transgenes of 1.1 kb and 1.3 kb were functional, resulting in resistant or recovery phenotype, while that of 5.8 kb was not functional. Copy number apparently had no effects on the phenotypes.

scholarly journals Nucleotide sequence of the potato leafroll virus coat protein gene

1989 ◽  
Vol 17 (4) ◽  
pp. 1768-1768 ◽  
Author(s):  
B. Prill ◽  
E. Maiss ◽  
U. Timpe ◽  
R. Casper
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Potato Leafroll Virus ◽  
Virus Coat Protein ◽  
Virus Coat ◽  
Leafroll Virus

scholarly journals Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern Brazil

2011 ◽  
Vol 36 (2) ◽  
pp. 121-124 ◽  
Author(s):  
José Evando A. Beserra Jr. ◽  
Eduardo C. Andrade ◽  
Rosa F.R. Araújo Camarço ◽  
Aline K.Q. Nascimento ◽  
José Albérsio A. Lima
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Northeastern Brazil ◽  
Sequence Variability ◽  
Virus Isolates

scholarly journals Detection and coat protein gene characterization of an isolate of Grapevine virus B from corky bark-affected grapevines in Southern Brazil

2002 ◽  
Vol 27 (3) ◽  
pp. 279-284 ◽  
Author(s):  
OSMAR NICKEL ◽  
THOR V. M. FAJARDO ◽  
FRANCISCO J. L. ARAGÃO ◽  
CÉSAR M. CHAGAS ◽  
GILMAR B. KUHN
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Amino Acid Sequences ◽  
Grapevine Virus ◽  
Leaf Extracts ◽  
Western Blots ◽  
Gene Characterization ◽  
Gene Coding ◽  
Grapevine Virus B

An isolate of Grapevine virus B (GVB), obtained by indexing Vitis labrusca and V. vinifera grapevines on the indicator LN33, was transmitted mechanically to several Nicotiana species. The virus was partially purified from N. cavicola and the coat protein estimated at 23 kDa by SDS-PAGE. In negatively stained leaf extracts of experimentally inoculated N. cavicola and N. occidentalis, flexuous particles with cross banding were observed, predominantly measuring 750-770 x 12 nm, with a modal length of 760 nm. Decoration indicated a clear, positive reaction against AS-GVB. In DAS-ELISA, GVB was detected in N. cavicola and grapevine extracts, and Western blots showed homologous and cross reaction of GVB and GVA antisera with GVB coat protein. Using specific primers for GVB, a fragment of 594 bp, comprising the coat protein gene coding for 197 amino acids, was amplified by RT-PCR with viral RNA extracted from GVB-infected N. occidentalis. The nucleotide and the deduced amino acid sequences of the coat protein gene showed high identities with Italian and Japanese isolates of GVB.

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