scholarly journals First molecular detection of canine herpesvirus 1 (CaHV-1) in the Eastern Brazilian Amazon

2022 ◽  
Vol 23 ◽  
Author(s):  
Marcela dos Santos Castro ◽  
Maridelzira Betania Moraes David ◽  
Evonnildo Costa Gonçalves ◽  
Andrei Santos Siqueira ◽  
Rodrigo Rodrigues Virgulino ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Brazilian Amazon ◽  
Canine Herpesvirus ◽  
Herpesvirus 1

scholarly journals Detection of Canine herpesvirus 1 in the breeding kennel and farm dogs by Real-time PCR

2020 ◽  
Author(s):  
Mahdieh Rezaei ◽  
Maziar Jajarmi ◽  
Saba Kamani ◽  
Mohammad Khalili ◽  
Homayoon Babaei
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Molecular Detection ◽  
Predisposing Factors ◽  
Reproductive Disorders ◽  
Widespread Distribution ◽  
Canine Herpesvirus ◽  
Herpesvirus 1 ◽  
Fertility Problems ◽  
Breeding Kennel

Abstract Background: Canine herpesvirus 1 (CHV-1) is known as a causal agent of death in newborn puppies and fertility problems in adults with a widespread distribution. There has been an increasing concern among dog breeders in Iran regarding CHV-1. This study is the first molecular detection of CHV-1 in breeding kennels and farm dogs in Iran using real-time PCR and investigation of various predisposing factors. Results: A total of 63 vaginal samples collected from 47 breeding kennels and 16 farm dogs were evaluated. In general, 21 out of 63 (33.3%) of vaginal samples were CHV-1-positive. The percentage of infection was higher in farm dogs, which was statistically significant. There was no significant association regarding other probable predisposing factors, including age, breed, pregnancy, and reproductive disorders. Conclusions: Considering the results of this study, CHV-1 is common in farm and breeding kennels and could pose a threat. Further studies are required to better understanding the distribution of the virus within Iran to advise dog breeders to more appropriate measures for management of CHV-1.

scholarly journals The first molecular detection of Canine herpesvirus-1 in reproductive specimens of adult dogs in Iran

2019 ◽  
Author(s):  
Mahdieh Rezaei ◽  
Maziar Jajarmi ◽  
Ramin Alizadeh ◽  
Mohammad Khalili ◽  
Homayoon Babaei
Keyword(s):  
Neonatal Death ◽  
Molecular Detection ◽  
Definitive Diagnosis ◽  
Molecular Assay ◽  
Reproductive Disorders ◽  
Viral Dna ◽  
Canine Herpesvirus ◽  
Management Measures ◽  
Herpesvirus 1 ◽  
Veterinary Hospital

Abstract Background Canine herpesvirus-1 (CHV-1) is recognized to be enzootic in the dog population with a widespread distribution. This pathogen leads to a lethal generalized illness in newborn puppies and is associated with reproductive disorders. CHV-1 should be considered as an important pathogen of neonatal death and infertility; so, it appears to pose a threat for breeding kennels. Although serologic data point to the circulation of CHV-1 among dogs of Iran, not definitive diagnosis has been conducted based on the molecular assay. So, this research was done to detect the prevalence of CHV-1 in dogs of Kerman. In this study, the presence of CHV-1 in vaginal specimens and biopsies of the uterus of dogs referring to the Veterinary Hospital of Shahid Bahonar University of Kerman was determined. Samples were collected and evaluated using real-time PCR.Results Viral DNA was detected in 21 samples from a total of 140 (15%) collected samples which were related to 14 uterine samples (20%) and 7 (10%) vaginal specimens. The association of this virus with age, breed, housing, pregnancy and reproductive disorders was not significant.Conclusions This study is the first molecular detection of CHV-1 in reproductive samples of dogs in Iran. Considering the significant prevalence of this virus, it is necessary to carry out management measures in controlling and preventing this disease. Tracing CHV-1 requires further research on this virus in dogs of this region.

Canine herpesvirus-1 (CHV-1): clinical, serological and virological patterns in breeding colonies

2005 ◽  
Vol 64 (1) ◽  
pp. 61-74 ◽  
Author(s):  
Veerle Ronsse ◽  
John Verstegen ◽  
Etienne Thiry ◽  
Karine Onclin ◽  
Christine Aeberlé ◽  
...  
Keyword(s):  
Canine Herpesvirus ◽  
Herpesvirus 1

scholarly journals Attachment and Penetration of Canine Herpesvirus 1 in Non-Permissive Cells.

2000 ◽  
Vol 62 (9) ◽  
pp. 965-970 ◽  
Author(s):  
Kazuo NAKAMICHI ◽  
Kentaro OHARA ◽  
Yasunobu MATSUMOTO ◽  
Haruki OTSUKA
Keyword(s):  
Canine Herpesvirus ◽  
Herpesvirus 1

Effects of ocular surface strontium-90 beta radiotherapy in dogs latently infected with canine herpesvirus-1

2014 ◽  
Vol 174 (3-4) ◽  
pp. 433-437 ◽  
Author(s):  
Amanda M. Nicklin ◽  
Margaret C. McEntee ◽  
Eric C. Ledbetter
Keyword(s):  
Ocular Surface ◽  
Canine Herpesvirus ◽  
Strontium 90 ◽  
Latently Infected ◽  
Herpesvirus 1

Seroprevalence of Canine Herpesvirus-1 in the Belgian Dog Population in 2000

2002 ◽  
Vol 37 (5) ◽  
pp. 299-304 ◽  
Author(s):  
V Ronsse ◽  
J Verstegen ◽  
K Onclin ◽  
AL Guiot ◽  
C Aeberle ◽  
...  
Keyword(s):  
Canine Herpesvirus ◽  
Herpesvirus 1

scholarly journals Zebra-borne neurotropic equid herpesvirus 1 meningoencephalitis in a Thomson’s gazelle (Eudorcas thomsonii)

2017 ◽  
Vol 29 (4) ◽  
pp. 548-556 ◽  
Author(s):  
Kanako Sakaguchi ◽  
Kenneth Kim ◽  
Ingeborg Langohr ◽  
Annabel G. Wise ◽  
Roger K. Maes ◽  
...  
Keyword(s):  
Inclusion Bodies ◽  
Gene Sequence ◽  
Molecular Detection ◽  
Specific Primer ◽  
Molecular Features ◽  
Equid Herpesvirus ◽  
Glycoprotein G ◽  
Microscopic Evaluation ◽  
Antigen Localization ◽  
Herpesvirus 1

We describe the histopathologic, immunohistochemical, and molecular features of a case of meningoencephalitis in a Thomson’s gazelle ( Eudorcas thomsonii) naturally infected with zebra-borne equid herpesvirus 1 (EHV-1) and the implications for the molecular detection of zebra-borne EHV-1. A 4-y-old female Thomson’s gazelle was submitted for postmortem examination; no gross abnormalities were noted except for meningeal congestion. Microscopic evaluation demonstrated multifocal nonsuppurative meningoencephalitis with intranuclear eosinophilic and amphophilic inclusion bodies and EHV-9 antigen in neurons. PCR demonstrated the presence of a herpesvirus with a nucleotide sequence 99–100% identical to the corresponding sequences of zebra-borne EHV-1 and of EHV-9 strains. To determine whether EHV-1 or EHV-9 was involved, a PCR with a specific primer set for EHV-9 ORF59/60 was used. The sequence was identical to that of 3 recognized zebra-borne EHV-1 strains and 91% similar to that of EHV-9. This isolate was designated as strain LM2014. The partial glycoprotein G ( gG) gene sequence of LM2014 was also identical to the sequence of 2 zebra-borne EHV-1 strains (T-529 isolated from an onager, 94-137 from a Thomson’s gazelle). The histologic lesions of encephalitis and antigen localization in this gazelle indicate prominent viral neurotropism, and lesions were very similar to those seen in EHV-1– and EHV-9–infected non-equid species. Histologic lesions caused by EHV-9 and zebra-borne EHV-1 are therefore indistinguishable.

Corneal ulceration associated with naturally occurring canine herpesvirus-1 infection in two adult dogs

2006 ◽  
Vol 229 (3) ◽  
pp. 376-384 ◽  
Author(s):  
Eric C. Ledbetter ◽  
Ronald C. Riis ◽  
Thomas J. Kern ◽  
Nicholas J. Haley ◽  
Scott J. Schatzberg
Keyword(s):  
Corneal Ulceration ◽  
Canine Herpesvirus ◽  
Naturally Occurring ◽  
Herpesvirus 1

Experimental reactivation of latent canine herpesvirus-1 and induction of recurrent ocular disease in adult dogs

2009 ◽  
Vol 138 (1-2) ◽  
pp. 98-105 ◽  
Author(s):  
Eric C. Ledbetter ◽  
Sung G. Kim ◽  
Edward J. Dubovi ◽  
Rodrigo C. Bicalho
Keyword(s):  
Ocular Disease ◽  
Canine Herpesvirus ◽  
Herpesvirus 1
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