scholarly journals Combination of multiplex reverse transcription recombinase polymerase amplification assay and capillary electrophoresis provides high sensitive and high-throughput simultaneous detection of avian influenza virus subtypes

2020 ◽  
Vol 21 (2) ◽  
Author(s):  
Shou-Kuan Tsai ◽  
Chen-Chih Chen ◽  
Han-Jia Lin ◽  
Han-You Lin ◽  
Ting-Tzu Chen ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Capillary Electrophoresis ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
High Throughput ◽  
Reverse Transcription ◽  
Simultaneous Detection ◽  
Recombinase Polymerase Amplification ◽  
Amplification Assay ◽  
Recombinase Polymerase Amplification Assay

scholarly journals Reverse‐transcription recombinase‐aided amplification assay for H7 subtype avian influenza virus

2019 ◽  
Vol 67 (2) ◽  
pp. 877-883 ◽  
Author(s):  
Suchun Wang ◽  
Baoxu Huang ◽  
Xuejun Ma ◽  
Ping Liu ◽  
Yang Wang ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Reverse Transcription ◽  
Amplification Assay ◽  
H7 Subtype

A Fully Automated Procedure for the High-Throughput Detection of Avian Influenza Virus by Real-Time Reverse Transcription–Polymerase Chain Reaction

2007 ◽  
Vol 51 (s1) ◽  
pp. 235-241 ◽  
Author(s):  
Montserrat Agüero ◽  
Elena San Miguel ◽  
Azucena Sánchez ◽  
Concepción Gómez-Tejedor ◽  
Miguel Angel Jiménez-Clavero
Keyword(s):  
Polymerase Chain Reaction ◽  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Real Time ◽  
High Throughput ◽  
Reverse Transcription ◽  
Chain Reaction ◽  
High Throughput Detection ◽  
Polymerase Chain

scholarly journals Reverse-transcription recombinase-aided amplification assay for H5 subtype avian influenza virus

2020 ◽  
Author(s):  
Suchun Wang ◽  
Yang Li ◽  
Nan Jiang ◽  
Fuyou Zhang ◽  
Qingye Zhuang ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Reverse Transcription ◽  
Economic Losses ◽  
Clinical Samples ◽  
Analytical Sensitivity ◽  
Single Temperature ◽  
Amplification Assay ◽  
H5 Subtype

AbstractThe H5 subtype Avian Influenza Virus has caused huge economic losses to the poultry industry and is a threat to human health. A rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks. In this study, we developed a reverse-transcription recombinase-aided amplification assay for the detection of H5 subtype avian influenza virus. Assays were performed at a single temperature (39°C), and the results were obtained within 20 min. The assay showed no cross-detection with Newcastle disease virus or infectious bronchitis virus. The analytical sensitivity was 103 RNA copies per reaction at a 95% confidence interval according to probit regression analysis, with 100% specificity. Compared with published reverse-transcription quantitative real-time polymerase chain reaction assays, the κ value of the reverse transcription recombinase-aided amplification assay in 365 avian clinical samples was 0.970 (p < 0.001). The sensitivity for avian clinical sample detection was 94.44% (95%CI, 70.63% - 99.71%), and the specificity was 100% (95%CI, 98.64% - 100%). These results indicated that our reverse-transcription recombinase-aided amplification assay may be a valuable tool for detecting H5 subtype avian influenza virus.

Sign in / Sign up

Export Citation Format

Share Document