scholarly journals Generation and protective efficacy of a cold-adapted attenuated genotype 2b porcine epidemic diarrhea virus

2019 ◽  
Vol 20 (4) ◽  
Author(s):  
Hokeun Won ◽  
Dong-Uk Lee ◽  
Guehwan Jang ◽  
Yun-Hee Noh ◽  
Seung-Chul Lee ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Protective Efficacy ◽  
Cold Adapted ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals Parenterally Administered Porcine Epidemic Diarrhea Virus-Like Particle-Based Vaccine Formulated with CCL25/28 Chemokines Induces Systemic and Mucosal Immune Protectivity in Pigs

Viruses ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 1122
Author(s):  
Chin-Wei Hsu ◽  
Ming-Hao Chang ◽  
Hui-Wen Chang ◽  
Tzong-Yuan Wu ◽  
Yen-Chen Chang
Keyword(s):  
Cellular Immunity ◽  
Porcine Epidemic Diarrhea Virus ◽  
Protective Efficacy ◽  
Clinical Signs ◽  
Effective Vaccine ◽  
Control Group ◽  
Diarrhea Virus ◽  
Viral Shedding ◽  
Specific Igg ◽  
Porcine Epidemic Diarrhea

Generation of a safe, economical, and effective vaccine capable of inducing mucosal immunity is critical for the development of vaccines against enteric viral diseases. In the current study, virus-like particles (VLPs) containing the spike (S), membrane (M), and envelope (E) structural proteins of porcine epidemic diarrhea virus (PEDV) expressed by the novel polycistronic baculovirus expression vector were generated. The immunogenicity and protective efficacy of the PEDV VLPs formulated with or without mucosal adjuvants of CCL25 and CCL28 (CCL25/28) were evaluated in post-weaning pigs. While pigs intramuscularly immunized with VLPs alone were capable of eliciting systemic anti-PEDV S-specific IgG and cellular immunity, co-administration of PEDV VLPs with CCL25/28 could further modulate the immune responses by enhancing systemic anti-PEDV S-specific IgG, mucosal IgA, and cellular immunity. Upon challenge with PEDV, both VLP-immunized groups showed milder clinical signs with reduced fecal viral shedding as compared to the control group. Furthermore, pigs immunized with VLPs adjuvanted with CCL25/28 showed superior immune protection against PEDV. Our results suggest that VLPs formulated with CCL25/28 may serve as a potential PEDV vaccine candidate and the same strategy may serve as a platform for the development of other enteric viral vaccines.

scholarly journals Evaluation of the Efficacy of Lactogenic Immunity of Sow Induced by Porcine Epidemic Diarrhea Virus (PEDV) Vaccine By Intranasal Immunization

2021 ◽  
Author(s):  
Shengchao Deng ◽  
Juan Wang ◽  
Mudassar Mohiuddin ◽  
Lisai Zhu ◽  
Guiping Wang ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Protective Efficacy ◽  
Virulent Strain ◽  
Passive Immunization ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diarrhea Virus ◽  
Suckling Piglets ◽  
Porcine Epidemic Diarrhea ◽  
Lactogenic Immunity ◽  
Highly Virulent

Abstract Background: Porcine epidemic diarrhea virus belongs to family of coronaviruses which are notorious for rapid spread of severe diarrhea among suckling piglets. The virus mainly replicates in the epithelial cells of duodenum, jejunum, ileum and colon and is a life threatening condition in pigs. A highly virulent strain “CHYJ130330” having high mortality rate was isolated from a field outbreak, identified as a new virulent genotype II/G2-b strain and adapted successfully to vero cells was used to prepare inactivated vaccine against PEDV. This newly prepared vaccine was given through intranasal route and is compared with the commercially available bi-combined (PEDV and TGEV) vaccine given by intramuscular injection. In this study milk or mucosal IgA and IgG antibody levels have been used to predict vaccine efficacy and the level of protective immunity against PED virus. Antibody titers in the milk of sows and intestines of suckling piglets were compared by enzyme-linked immunosorbent assay (ELISA). Results: It was shown that CHYJ vaccine induced significantly higher levels of PEDV IgA antibody in milk of sows and intestines of piglets as compared to commercial bi-combined vaccine. Both CHYJ and commercial vaccines were not able to induce detectable IgG levels in the intestines of piglets; the later however induced higher IgG levels when detected in the sow’s milk. Protective efficacy of vaccines was determined against a highly virulent PEDV strain. CHYJ intranasal vaccine gives a better protection 80% (4/5) rate as compared to commercial i.m. vaccine conferring 60% (3/5) immunity in suckling piglets. Conclusions: It is therefore concluded that PEDV inactivated CHYJ vaccine confer better lactogenic immunity and gives more protection to suckling piglets than available bi-combined TGEV and PEDV vaccine through passive immunization.

scholarly journals Identifying outbreaks of Porcine Epidemic Diarrhea virus through animal movements and spatial neighborhoods

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Gustavo Machado ◽  
Carles Vilalta ◽  
Mariana Recamonde-Mendoza ◽  
Cesar Corzo ◽  
Montserrat Torremorell ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Animal Movements ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

PSVI-4 Efficacy of Medium Chain Fatty Acids and Fatty Acid-based Feed Additives as a Mitigation Strategy Against Porcine Epidemic Diarrhea Virus and Porcine Reproductive and Respiratory Syndrome Virus

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 216-217
Author(s):  
O L Harrison ◽  
G E Nichols ◽  
J T Gebhardt ◽  
Cassandra K Jones ◽  
Jason C Woodworth ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Porcine Epidemic Diarrhea Virus ◽  
Positive Control ◽  
Feed Additives ◽  
Feed Additive ◽  
Respiratory Syndrome Virus ◽  
Post Inoculation ◽  
Diarrhea Virus ◽  
Medium Chain ◽  
Porcine Epidemic Diarrhea

Abstract Recent research has demonstrated that swine viruses can be transmitted via feed. Chemical feed additives have been suggested for the mitigation of these viruses in complete feed. Therefore, the objective of this study was to evaluate the efficacy of a commercially available formaldehyde-based feed additive, medium chain fatty acid blend (MCFA), and commercially available fatty acid-based products for mitigation of porcine epidemic diarrhea virus (PEDV) and porcine reproductive and respiratory syndrome virus (PRRSV) in a feed matrix. Treatments consisted of: 1) non-treated positive control, 2) 0.33% commercial formaldehyde-based product (Sal Curb; Kemin Industries, Inc.; Des Moines, IA), 3) 0.5% MCFA blend (1:1:1 ratio of C6:0, C8:0, and C10:0, Sigma Aldrich, St. Louis, MO), 4) 0.25%, 5) 0.5%, or 6) 1% of commercial dry mono and diglyceride-based product (Furst Strike; Furst-McNess Company, Freeport, IL), 7) 0.25%, 8) 0.5%, or 9) 1% of commercial dry mono and diglyceride-based product (Furst Protect; Furst-McNess Company, Freeport, IL), 10) 0.25%, 11) 0.5%, or 12) 1% dry mono and diglyceride-based experimental product (Furst-McNess Company, Freeport, IL) with 3 replications/treatment. Treatments were applied to complete swine feed before inoculation with 106 TCID50/g of feed with PEDV or PRRSV. Post inoculation feed was held at ambient temperature for 24 h before being analyzed via qRT-PCR. The analyzed values represent the cycle threshold. Formaldehyde and MCFA decreased (P < 0.05) the detectable RNA of PEDV and PRRSV compared to all other treatments. Furst Strike, Furst Protect, and the experimental product did not significantly impact detectability of PEDV or PRRSV RNA. In conclusion, MCFA and formaldehyde treatments are effective at reducing detection of RNA from PEDV and PRRSV in feed.

scholarly journals Differential expression and correlation analysis of miRNA–mRNA profiles in swine testicular cells infected with porcine epidemic diarrhea virus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiaoqian Zhang ◽  
Chang Li ◽  
Bingzhou Zhang ◽  
Zhonghua Li ◽  
Wei Zeng ◽  
...  
Keyword(s):  
Differential Expression ◽  
Porcine Epidemic Diarrhea Virus ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Bacterial Invasion ◽  
Sequencing Data ◽  
Diarrhea Virus ◽  
Comparison Groups ◽  
Porcine Epidemic Diarrhea

AbstractThe variant virulent porcine epidemic diarrhea virus (PEDV) strain (YN15) can cause severe porcine epidemic diarrhea (PED); however, the attenuated vaccine-like PEDV strain (YN144) can induce immunity in piglets. To investigate the differences in pathogenesis and epigenetic mechanisms between the two strains, differential expression and correlation analyses of the microRNA (miRNA) and mRNA in swine testicular (ST) cells infected with YN15, YN144, and mock were performed on three comparison groups (YN15 vs Control, YN144 vs Control, and YN15 vs YN144). The mRNA and miRNA expression profiles were obtained using next-generation sequencing (NGS), and the differentially expressed (DE) (p-value < 0.05) mRNA and miRNA were obtained using DESeq R package. mRNAs targeted by DE miRNAs were predicted using the miRanda algortithm. 8039, 8631 and 3310 DE mRNAs, and 36, 36, and 22 DE miRNAs were identified in the three comparison groups, respectively. 14,140, 15,367 and 3771 DE miRNA–mRNA (targeted by DE miRNAs) interaction pairs with negatively correlated expression patterns were identified, and interaction networks were constructed using Cytoscape. Six DE miRNAs and six DE mRNAs were randomly selected to verify the sequencing data by real-time relative quantitative reverse transcription polymerase chain reaction (qRT-PCR). Based on bioinformatics analysis, we discovered the differences were mostly involved in host immune responses and viral pathogenicity, including NF-κB signaling pathway and bacterial invasion of epithelial cells, etc. This is the first comprehensive comparison of DE miRNA–mRNA pairs in YN15 and YN144 infection in vitro, which could provide novel strategies for the prevention and control of PED.

scholarly journals Sequence analysis of new variants of porcine epidemic diarrhea virus in Luzon, Philippines, in 2017

2021 ◽  
Author(s):  
Saubel Ezrael A. Salamat ◽  
Therese Marie A. Collantes ◽  
Wenchie Marie L. Lumbera ◽  
Francis A. Tablizo ◽  
Christian Thomas M. Mutia ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Porcine Epidemic Diarrhea Virus ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea
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