scholarly journals Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples

2017 ◽  
Vol 18 (2) ◽  
pp. 253
Author(s):  
Hye Kwon Kim ◽  
Kwang Soo Lyoo ◽  
Thi My Le Huynh ◽  
Hyoung Joon Moon ◽  
Van Giap Nguyen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Simultaneous Detection ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Respiratory Syndrome Virus ◽  
Chain Reaction ◽  
Tissue Samples ◽  
Polymerase Chain

scholarly journals Histopathological and virological findings in emaciated pigs from Mexico: an exploratory study

2018 ◽  
Vol 87 (3) ◽  
pp. 213-217
Author(s):  
Aide Alpízar ◽  
Joaquim Segalés ◽  
Simón Martínez ◽  
Atalo Martínez ◽  
Guadalupe Socci ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Body Condition ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Economic Losses ◽  
Respiratory Syndrome Virus ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Histopathological Studies ◽  
Weaning Piglets

The objective of this work was to detect the presence of three main pig respiratory viral agents (porcine rubulavirus [PorPV], porcine circovirus type 2 [PCV-2], and porcine reproductive and respiratory syndrome virus [PRRSV]) in tissues of emaciated piglets from the Baj'o Region (Mexico). Necropsies and histopathological studies of 37 pigs with poor body condition were performed; viruses were detected by molecular biology methods and PCV-2 was further assessed by immunohistochemistry (IHC). Histopathologically, interstitial pneumonia was observed in 25/37 (68%) of the piglets. Also, a varying degree of lymphocyte depletion in lymphoid organs was found in 14/37 (38%) animals. Through polymerase chain reaction (PCR) and/or reverse transcription polymerase chain reaction (RT-PCR), from the 37 pigs, 16 were positive for PCV-2, 18 for PRRSV and 1 for PorPV. In accordance with these results, the infection and/or co-infection with PCV-2 and PRRSV were fairly frequent findings in piglets with poor body condition in Mexico, while the infection by PorPV was apparently negligible. Wasting of post-weaning piglets is a global pig farming problem that causes great economic losses and has been associated with diverse factors: microbial agents, environmental factors, nutritional factors, and management. When the Blue Eye Disease was first reported in Mexico, it was associated with severe wasting in post-weaning piglets. This study demonstrated that this disease does not seem to play such an important role in the wasting as was previously thought.

scholarly journals Survey Of Infectious Agents Associated With Porcine Respiratory Disease Complex (PRDC) In Serbian Swine Herds Using Polymerase Chain Reaction (PCR) Detection

2015 ◽  
Vol 65 (1) ◽  
pp. 79-88 ◽  
Author(s):  
SAVIĆ Božidar ◽  
RADANOVIĆ Oliver ◽  
JOVIČIĆ Dubravka ◽  
NEŠIĆ Ksenija ◽  
IVANOVIĆ Snežana ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Disease ◽  
Respiratory Virus ◽  
Mycoplasma Hyopneumoniae ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Porcine Respiratory

Abstract A retrospective study on 235 natural cases of Porcine Respiratory Disease Complex in order to determine the etiological agents, their prevalence and interrelationships was performed in Serbia. Lung tissue samples were analyzed by Polymerase Chain Reaction for the presence of Porcine circovirus type 2, Porcine reproductive and respiratory virus, Swine influenza virus, Mycoplasma hyopneumoniae, Pasteurella multocida, Actinobacillus pleuropneumoniae, Haemophilus parasuis, Streptococcus suis and Arcanobacterium pyogenes. A total of 49 different combinations of viral and bacterial pathogens were found. Five different viral and viral/Mhp co-infections were detected. Monobacterial infections were found in 150 cases and polybacterial infection was detected in 85 samples. PCV2 was the main virus detected, and Pm was the most aggressive secondary pathogen detected in PRDC. The reason for PRDC being so prevalent among Serbian pigs is most likely due to the large number of risk factors in the conventional farrow-to-finish system, compared to multi-site production systems. Therefore, measures aimed at a better control of respiratory viruses, particularly Porcine circovirus type 2 and Porcine reproductive and respiratory virus, as well as Mycoplasma hyopneumoniae infections, and adoption of rational decisions on respiratory bacterial pathogens specific therapeutic and preventive strategies at herd level, simultaneously with significant improvements on farm management should reduce the occurrence of PRDC.

scholarly journals Detection of Porcine Circovirus Type 2 in Feces of Pigs with or without Enteric Disease by Polymerase Chain Reaction

2003 ◽  
Vol 15 (4) ◽  
pp. 369-373 ◽  
Author(s):  
Jeong S. Yang ◽  
Dae S. Song ◽  
So Y. Kim ◽  
Kwang S. Lyoo ◽  
Bong K. Park
Keyword(s):  
Polymerase Chain Reaction ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Enteric Disease ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Fecal Samples ◽  
Polymerase Chain

To establish the sensitive polymerase chain reaction(PCR) method and detect porcine circovirus type 2 (PCV2) from intestines and feces of commercial swine herds with or without enteric disease, intestinal samples from 68 pigs and 29 fecal samples from commercial swine farms were collected. A primer set, forward primer 5′-GAAGAATGGAAGAAGCGG-3′ and reverse primer 5′-CTCACAGCAGTAGACAGGT-3′, could detect the virus at a concentration as low as 2 infectious virions per milliliter under controlled conditions using PK-15 cell-adapted PCV2. The genomic nucleotide sequences of open reading frame 1 (ORF1) PCR products from fecal samples were found to have complete homology with other PCV2s deposited in the GenBank database. Transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PEDV) as the other enteric pathogens were also investigated by performing duplex reverse transcription-PCR (RT-PCR). Among 63 pigs with clinical enteric disease, 18 PCV2s (14 from intestines and 4 from feces), 7 TGEVs from intestines, and 18 PEDVs (14 from intestines and 4 from feces) were detected by PCR and the duplex RT-PCR. In 34 pigs (14 from intestines and 20 from feces) without clinical enteric disease, only PCV2 was detected in 19 pigs (3 from intestines and 16 from feces). Both PEDV and PCV2 were found in 6 pigs with clinical enteric disease. Among 15 PCV2 samples that were PCR-positive, 4 were culture-positive at passage level 3 in PK-15 cells. These results reveal that PCV2 is shed through the feces of pigs without clinical enteric disease, which suggests the potentiality of the fecal–oral transmission of PCV2 in feces.

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