scholarly journals Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cellsin vitromodel

2016 ◽  
Vol 17 (2) ◽  
pp. 145 ◽  
Author(s):  
Xiao-ying He ◽  
Li-bing Ma ◽  
Xiao-ning He ◽  
Wan-tong Si ◽  
Yue-Mao Zheng
Keyword(s):  
Mammary Gland ◽  
Somatic Cell ◽  
Bovine Embryos ◽  
Cloned Bovine

55 EFFECT OF FUSION-ACTIVATION INTERVAL AND EMBRYO AGGREGATION ON IN VITRO AND IN VIVO DEVELOPMENT OF CLONED BOVINE EMBRYOS PRODUCED BY HANDMADE CLONING

2010 ◽  
Vol 22 (1) ◽  
pp. 185
Author(s):  
R. P. C. Gerger ◽  
F. Forell ◽  
J. C. Mezzalira ◽  
F. Zago ◽  
F. K. Vieira ◽  
...  
Keyword(s):  
Somatic Cell ◽  
High Rate ◽  
Bovine Embryos ◽  
In Vitro Development ◽  
Cloned Bovine ◽  
Vitro Development ◽  
Handmade Cloning ◽  
Activation Intervals

Despite the apparent success of cloning by somatic cell nuclear transfer (SCNT), the efficiency in development to term remains low, with a high rate of losses occurring throughout pregnancy due to faulty reprogramming and conceptus abnormalities. As the ideal fusion-activation interval for optimal nuclear reprogramming after cloning is still ill-defined, the aim of this study was to determine the effect of 2 distinct fusion-activation intervals and embryo aggregation on in vitro development of cloned bovine embryos. Bovine COCs from slaughterhouse ovaries were used after IVM for the production of cloned embryos by handmade cloning, according to our established procedures (Ribeiro et al. 2009 Cloning Stem Cells, in press). Following cumulus and zona removal, oocytes were manually bisected, with hemi-cytoplasts selected by DNA staining. Two hemi-cytoplasts and an adult skin somatic cell were attached and fused with a 15V AC pre-pulse for 5 s, followed by a double 1.2 kV cm-1 DC pulse for 20 μs. Reconstructed embryos were activated in ionomycin exactly at 2 or 4 h post-fusion (2 hpf or 4 hpf), followed by an incubation in 6-DMAP for 4 h. Cloned embryos from both fusion-activation intervals were in vitro-cultured in the well of the well (WOW) system for 7 days, allocating one (1 × 100%) or two (2 × 100%) cloned embryos per WOW. Grade 1 Day-7 blastocysts were transferred to synchronous recipients. Cleavage (Day 2) and blastocyst (Day 7) rates, on a per WOW basis, and pregnancy (Days 30 and 150) rates were compared using the chi-square or the Fisher test, with results from 9 replications summarized in Table 1. Increasing the fusion-activation interval to 4 h decreased cleavage but not blastocyst rates in 1 × 100% embryos. Also, blastocyst rates were lower in 1 × 100% embryos activated 2 h post-fusion. In general, cleavage and blastocysts rates for 2 × 100% embryos (91.5 and 46.0%) were higher than for 1 × 100% embryo counterparts (74.4 and 31.3%), respectively, regardless of the activation time. In addition, blastocyst rates for 4 hpf-activated embryos (50.3%), based on cleavage, were higher than for 2 hpf-activated embryos (38.3%), irrespective of the aggregation scheme. Nonetheless, despite differences in in vitro development, pregnancy rates and conceptus development in the first half of pregnancy were similar between groups. A longer fusion-activation interval (4 hpf) or embryo aggregation (2 × 100%) increased blastocyst yield but did not improve in vivo development and pregnancy maintenance following the transfer to female recipients in cattle. Table 1.In vitro and in vivo development of cloned bovine embryos This study was supported by FAPESP and CAPES, Brazil.

Effects of Insulin-like Growth Factor-1 on Development of Somatic Cell Cloned Bovine Embryos

2016 ◽  
Vol 18 (3) ◽  
pp. 162-170 ◽  
Author(s):  
Pengxiang Qu ◽  
Yanyan Li ◽  
Tengfei Deng ◽  
Dan Jia ◽  
Suzhu Qing ◽  
...  
Keyword(s):  
Growth Factor ◽  
Somatic Cell ◽  
Insulin Like Growth Factor ◽  
Bovine Embryos ◽  
Cloned Bovine

scholarly journals Transcriptional reprogramming of somatic cell nuclei during preimplantation development of cloned bovine embryos

2007 ◽  
Vol 305 (2) ◽  
pp. 637-649 ◽  
Author(s):  
Zeki Beyhan ◽  
Pablo J. Ross ◽  
Amy E. Iager ◽  
Arif M. Kocabas ◽  
Kerrianne Cunniff ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Preimplantation Development ◽  
Bovine Embryos ◽  
Cell Nuclei ◽  
Transcriptional Reprogramming ◽  
Cloned Bovine

Production of cloned bovine embryos by somatic cell transfer into enucleated zona-free oocytes

2006 ◽  
Vol 33 (3) ◽  
pp. 224-230
Author(s):  
G. P. Malenko ◽  
M. I. Prokof’ev ◽  
M. V. Pinyugina ◽  
T. A. Antipova ◽  
M. N. Mezina ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Transfer ◽  
Bovine Embryos ◽  
Cloned Bovine

Effects of the removal of cytoplasm on the development of early cloned bovine embryos

2011 ◽  
Vol 126 (1-2) ◽  
pp. 37-44 ◽  
Author(s):  
Song Hua ◽  
Hui Zhang ◽  
Jian Min Su ◽  
Tuo Zhang ◽  
Fu Sheng Quan ◽  
...  
Keyword(s):  
Bovine Embryos ◽  
Cloned Bovine

scholarly journals The Effect of Pre-milking Stimulation on Teat Morphological Parameters and Milk Traits in the Italian Water Buffalo

2020 ◽  
Vol 7 ◽  
Author(s):  
Angela Costa ◽  
Massimo De Marchi ◽  
Giulio Visentin ◽  
Maria Concetta Campagna ◽  
Antonio Borghese ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Somatic Cell ◽  
Milk Yield ◽  
Large Scale ◽  
Chloride Concentration ◽  
Morphological Characteristics ◽  
Health Indicators ◽  
Milk Traits ◽  
Manual Stimulation ◽  
The Impact

Water buffaloes (Bubalus bubalis) are very sensitive to environmental stimulus before and during milking, and this explains why disrupted milk ejections due to blood oxytocin level instability are frequent in this species. According to the literature, the manual stimulation (MS) of teats before milking promotes oxytocin release and allows milk ejection to start within 2–3 min. However, the pre-milking stimulation of teats is not always part of the milking routine in Italian buffalo farms; moreover, buffaloes with unstable milk let-down are sometimes treated with exogenous oxytocin (OX). Different types of pre-milking stimulation can impact differently on the mammary gland epithelium and structures and, therefore, on milk yield. In this study, we observed the changes in teat morphological traits before and after the application of three types of stimuli, i.e., no manual stimulation (NS), MS, and OX, in buffaloes reared in an Italian dairy farm. In particular, measurements were available for 23 and 21 buffaloes for front and rear teats, respectively. Subsequently, the effect of the pre-milking stimulation type was estimated on teat morphological characteristics and on milk traits recorded after the application of stimuli. The results showed that the teat canal length was shorter (P < 0.05) after stimulation in the case of MS and OX compared to NS. Cistern diameter was overall greater for MS and lower for OX. On the contrary, teat wall thickness was greater and lower for OX and MS, respectively. Milk yield and quality (fat, protein, and somatic cell score) were similar across the three types of pre-milking stimulation. In perspective, the impact of these types of pre-milking stimulation should be evaluated on a large scale, and the focus might be put on mammary gland epithelium integrity, mastitis incidence, and other udder health indicators in milk, e.g., electrical conductivity, differential somatic cell count, lactose content, and sodium and chloride concentration.

Aberrant methylation of donor genome in cloned bovine embryos

10.1038/88903 ◽  
2001 ◽  
Vol 28 (2) ◽  
pp. 173-177 ◽  
Author(s):  
Yong-Kook Kang ◽  
Deog-Bon Koo ◽  
Jung-Sun Park ◽  
Young-Hee Choi ◽  
An-Sik Chung ◽  
...  
Keyword(s):  
Aberrant Methylation ◽  
Bovine Embryos ◽  
Donor Genome ◽  
Cloned Bovine
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