Rapid detection of rusty grain beetles (Coleoptera: Cucujidae) from wheat samples passing through a mechanical dockage tester

1997 ◽  
Vol 77 (4) ◽  
pp. 717-719 ◽  
Author(s):  
C. J. Demianyk ◽  
N. D. G. White ◽  
D. S. Jayas

A dockage tester (Carter dockage tester, Hart-Carter Co., Minneapolis, MN) was set up to clean wheat in accordance to Canadian grain industry guidelines and several running conditions. Under all conditions, 84–91% of adults and 57–81% of larvae of the rusty grain beetle, Cryptolestes ferrugineus (Stephens) (Coleoptera: Cucujidae) were recovered from infested 1-kg samples in the aspirator discharge pan. All immature insects implanted in the germ of wheat kernels remained within the kernels, and were found within the bulk of the cleaned whole wheat. It is therefore possible to monitor the aspirator discharge pan of cleaned wheat samples for rapid detection of most free-living C. ferrugineus to limit infested grain from entering primary elevators. Key words: Cryptolestes, detection, dockage tester, Insecta, rusty grain beetle, wheat

2014 ◽  
pp. 87-92
Author(s):  
Thi Hoai Nguyen ◽  
Thi Van Thi Tran ◽  
Trung Hieu Le ◽  
Thi Mai Huong Vo

Background: There are many beneficial effects such as reducing the risk of obesity, diabetes, hyperlipidemia and hypercholesterolemia from Amorphophallus sp. This reports are research results of physicochemical properties of glucomannan flour from tubers of Amorphophallus paeoniifolius cultivated in Thua Thien Hue. Materials: Glucomannan flour from tubers of Amorphophallus paeoniifolius (Dennst) Nicolson – Araceae cultivated in Thua Thien Hue. Method: Identify the quantity and physicochemical properties by many methods such as using enzymes, chemistry, physical chemistry, spectroscopic methods, laser analysis. Results: Identified starch and glucomannan quantity, physicochemical properties and indicators of microbiological of glucomannan flour. Conclusion: From the achieved results set up quality standards of glucomannan flour from tubers of Amorphophallus paeoniifolius cultivated in Thua Thien Hue. Key words: Glucomannan, starch, β-amylase.


2018 ◽  
Vol 6 (2) ◽  
pp. 14
Author(s):  
Darmawan Darmawan ◽  
Jajang Setiawan

<p align="center"><strong>ABSTRACT</strong><strong></strong></p><p align="center"><strong> </strong></p><p align="justify"><em>Today, the growth of economic level begins to rise again that is generated by the increase of   different kind of product and different kind of businesses that are offered by both individual businesses that are done easly independently. To deal with all of business trends, the education institution has to prepare the students to be able to set up a business idenpendedntly through the subject of entrepeuneurship.  As it is regulated in Vision and Mision of study program and is also regulated in the curriculum of lectures. </em><em></em></p><p align="justify"><em>This research is aimed at to find out the correlation between the spirit of entrepeunership and motivation , Family background, and education level. Based on the research result, the finding showed that the spirit of entrepeneurship correlated with the motivation and family background. Based on the finding, it was also found that education level did not correlate with that the spirit of entrepeneurship. In regard with the findings, it is important to develop more the motivation of students to touch up the sperit of entrepenuership. </em><em></em></p><p align="justify"> </p><p><em>Key words: Entrepenuer, Students, Entrepeuneurship. </em></p>


Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1619
Author(s):  
Lucia Bartella ◽  
Fabio Mazzotti ◽  
Ines Rosita Talarico ◽  
Ilaria Santoro ◽  
Leonardo Di Di Donna

A new original process has been set-up to obtain hydroxytyrosol-enriched food by means of CO2 supercritical fluid extraction. The process is based on the direct adsorption of hydroxytyrosol on selected foodstuff (e.g., flour, whole-wheat flour, and sugar) when placed in contact with olive oil (which is known to contain this phenol) under controlled temperature and pressure conditions of supercritical CO2. The transfer of the nutraceutical to the foodstuff was initially evaluated using a fortified olive oil model and was then applied to commercial olive oil and foodstuff. The yield of the hydroxytyrosol transfer was demonstrated to be quantitative. In order to prove the suitability of the final products, hydroxytyrosol-enriched flour was used to prepare a bread roll, which maintained the nutraceutical characteristics after the cooking. Finally, DPPH based experiments were performed to prove the radical scavenging activity of the functionalized foodstuff.


2009 ◽  
pp. 129-136
Author(s):  
Teresa Babuscio

- Food controls are a key issue within the European food safety arena. Since 2002 the legislator is constantly improving the control system in order to better achieve the high safety principles contained in the European regulation n.178 and the network set up in it. Thus, the regulation n. 882/2004 is specifically dedicated to food safety controls: it is the main piece of legislation concerning this aspect as it has been adopted by the co-decisions procedure which involves the European Parliament and the Council. Despite that, it left some "white" spaces to be filled by the implementing measures adopted within the so called comitology procedure in which it is the Commission to act as a co-regulator. The latter is currently implementing art. 15.5. of the regulation n. 882/2004 to set up a system of increased controls for food and feed. Key words: food safety; control system; regulation n. 882/2004.


2021 ◽  
Vol 7 (6) ◽  
pp. 6303-6316
Author(s):  
Weixi Gao ◽  
Yan Zhuang

in the detection of chloramphenicol residues in fermented food, there are often problems of slow detection speed. Using UPLC-DAD method, a rapid detection method of chloramphenicol residues in fermented food based on UPLC-DAD method is designed. According to the characteristics of chloramphenicol, set up the detection reagent, select the detection equipment, and form the detection laboratory. It is usingUPLC-DAD method to design the test paper, using the set test reagent to deal with the sample to be tested, according to the design results of the test process, combining the reagent with the sample, to determine its specificity. Chloramphenicol residue was detected by test paper. So far, the rapid detection method of chloramphenicol residues in fermented food based on UPLC-DAD method has been designed. Compared with the original detection method, the detection speed of the detection method designed in this paper is significantly higher than the original method. In conclusion, the rapid detection method of chloramphenicol residues in fermented food based on UPLC-DAD method is effective.


2020 ◽  
Vol 11 ◽  
Author(s):  
Luping Xu ◽  
Xingjian Bai ◽  
Shivendra Tenguria ◽  
Yi Liu ◽  
Rishi Drolia ◽  
...  

Rapid detection of live pathogens is of paramount importance to ensure food safety. At present, nucleic acid-based polymerase chain reaction and antibody-based lateral flow assays are the primary methods of choice for rapid detection, but these are prone to interference from inhibitors, and resident microbes. Moreover, the positive results may neither assure virulence potential nor viability of the analyte. In contrast, the mammalian cell-based assay detects pathogen interaction with the host cells and is responsive to only live pathogens, but the short shelf-life of the mammalian cells is the major impediment for its widespread application. An innovative approach to prolong the shelf-life of mammalian cells by using formalin was undertaken. Formalin (4% formaldehyde)-fixed human ileocecal adenocarcinoma cell line, HCT-8 on 24-well tissue culture plates was used for the capture of viable pathogens while an antibody was used for specific detection. The specificity of the Mammalian Cell-based ImmunoAssay (MaCIA) was validated with Salmonella enterica serovar Enteritidis and Typhimurium as model pathogens and further confirmed against a panel of 15 S. Enteritidis strains, 8 S. Typhimurium, 11 other Salmonella serovars, and 14 non-Salmonella spp. The total detection time (sample-to-result) of MaCIA with artificially inoculated ground chicken, eggs, milk, and cake mix at 1–10 CFU/25 g was 16–21 h using a traditional enrichment set up but the detection time was shortened to 10–12 h using direct on-cell (MaCIA) enrichment. Formalin-fixed stable cell monolayers in MaCIA provide longer shelf-life (at least 14 weeks) for possible point-of-need deployment and multi-sample testing on a single plate.


2013 ◽  
Vol 79 (20) ◽  
pp. 6407-6413 ◽  
Author(s):  
E. Lambrecht ◽  
J. Baré ◽  
I. Van Damme ◽  
W. Bert ◽  
K. Sabbe ◽  
...  

ABSTRACTFree-living protozoa play an important role in the ecology and epidemiology of human-pathogenic bacteria. In the present study, the interaction betweenYersinia enterocolitica, an important food-borne pathogen, and the free-living amoebaAcanthamoeba castellaniiwas studied. Several cocultivation assays were set up to assess the resistance ofY. enterocoliticatoA. castellaniipredation and the impact of environmental factors and bacterial strain-specific characteristics. Results showed that allY. enterocoliticastrains persist in association withA. castellaniifor at least 14 days, and associations withA. castellaniienhanced survival ofYersiniaunder nutrient-rich conditions at 25°C and under nutrient-poor conditions at 37°C. Amoebae cultivated in the supernatant of oneYersiniastrain showed temperature- and time-dependent permeabilization. Intraprotozoan survival ofY. enterocoliticadepended on nutrient availability and temperature, with up to 2.8 log CFU/ml bacteria displaying intracellular survival at 7°C for at least 4 days in nutrient-rich medium. Transmission electron microscopy was performed to locate theYersiniacells inside the amoebae. AsYersiniaandAcanthamoebashare similar ecological niches, this interaction identifies a role of free-living protozoa in the ecology and epidemiology ofY. enterocolitica.


1977 ◽  
Vol 109 (2) ◽  
pp. 209-213 ◽  
Author(s):  
J. M. Bryan ◽  
J. Elvidge

AbstractThe detection of insect infestation by the Canadian Grain Commission is decreased in efficiency by the mortality of adult beetles in the pneumatic sample delivery systems used in terminal elevators in the Vancouver, B.C., area. Dead insects are difficult to see while grading the grain and cannot be extracted by Berlese funnels.The level of mortality varied with the species of beetle and the system tested, but not with the type of grain. In one system the following mortalities were found: Cryptolestes ferrugineus (Stephens), 73%; Oryzaephilus surinamensis (L.), 65%; Tribolium castaneum (Herbst), 65%; and Sitophilus granarius (L.), 22%. C. ferrugineus, the most important insect infesting Canadian grain, sustained an average mortality of 55% in the five systems tested. No consistent mortality pattern was found among the systems with respect to delivery tube length, number of turns, or approximate grain speed. Berlese funnel extraction was found to be inefficient for O. surinamensis and inconsistent for S. granarius.


2014 ◽  
Vol 59 ◽  
pp. 292-298 ◽  
Author(s):  
G.D. Arlene-Christina ◽  
D.S. Jayas ◽  
P.G. Fields ◽  
F. Jian ◽  
N.D.G. White ◽  
...  

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