The inheritance of erucic acid content in Ethiopian mustard

1997 ◽  
Vol 77 (1) ◽  
pp. 33-41 ◽  
Author(s):  
A. Getinet ◽  
G. Rakow ◽  
J. P. Raney ◽  
R. K. Downey
Keyword(s):  
Erucic Acid ◽  
Acid Content ◽  
Acid Synthesis ◽  
Erucic Acid Content ◽  
Human Consumption ◽  
Oilseed Crop ◽  
High Erucic Acid ◽  
Ethiopian Mustard ◽  
Low Erucic Acid ◽  
Key Words:Brassica

Ethiopian mustard (Brassica carinata A. Braun) is a highly productive oilseed crop in the central highlands of Ethiopia. Cultivars currently in production in Ethiopia produce seed which contains 35–40% erucic acid in its oil which is undesirable for human consumption. Zero erucic acid B. carinata has recently been developed. The objective of this study was to investigate the inheritance of erucic acid in progeny of crosses between the high erucic acid cultivars Dodolla and S-67 with the zero erucic acid line C90-14. The erucic acid content of F1 seed born on either the high or low erucic acid parents was intermediate between the parents indicating embryonic control of erucic acid content in B. carinata. Erucic acid contents of backcross seed derived from the backcross to the zero erucic acid parent segregated into three classes with <0.5%, 6–16% and >16% erucic acid at a ratio of 1:2:1 and F2 seed segregated into five classes with a ratio of 1:4:6:4:1. These segregation patterns indicated that erucic acid in B. carinata was controlled by two genes acting in an additive manner with each locus contributing about 10% erucic acid. It was concluded that the B and C genomes of B. carinata each carry one gene for erucic acid synthesis. The knowledge of the inheritance of erucic acid in B. carinata will assist in the development of zero erucic acid B. carinata cultivars. Key words:Brassica carinata, erucic acid, inheritance

scholarly journals In Vitro Production of Somaclones with Decreased Erucic Acid Content in Indian Mustard [Brassica juncea (Linn.) Czern&Coss]

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1297
Author(s):  
Chitralekha Shyam ◽  
Manoj Tripathi ◽  
Sushma Tiwari ◽  
Niraj Tripathi ◽  
Ravindra Solanki ◽  
...  
Keyword(s):  
Erucic Acid ◽  
Acid Content ◽  
Indian Mustard ◽  
Mother Plant ◽  
Erucic Acid Content ◽  
Oilseed Crop ◽  
In Vitro Production ◽  
Somaclonal Variations ◽  
Low Erucic Acid

Brassica junceais a crucial cultivated mustard species and principal oilseed crop of India and Madhya Pradesh, grown for diverse vegetables, condiments, and oilseeds. Somaclonal variation was explored as a probable source of additional variability for the manipulation of fatty acids, especially low erucic acid contents that may be valuable for this commercially important plant species. The plantlets regenerated from tissue cultures (R0), their R1 generation and respective parental lines were compared for morpho-physiological traits and fatty acid profile for the probable existence of somaclonal variations. The first putative somaclone derived from genotype CS54 contained 5.48% and 5.52% erucic acid in R0 and R1 regenerants, respectively, compared to the mother plant (41.36%). In comparison, the second somaclone acquired from PM30 exhibited a complete absence of erucic acid corresponding to its mother plant (1.07%). These putative somaclones present a source of variation for exploitation in the development of future mustard crops with low erucic acid content.

GLC analysis of Indian rapeseed-mustard to study the variability of fatty acid composition

2000 ◽  
Vol 28 (6) ◽  
pp. 581-582 ◽  
Author(s):  
N. Kaushik ◽  
A. Agnihotri
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Erucic Acid ◽  
Linolenic Acid ◽  
Acid Composition ◽  
Acid Content ◽  
Erucic Acid Content ◽  
High Erucic Acid ◽  
Low Erucic Acid

Rapeseed-mustard is one of the most economically important oilseed crops in India. Speciality oils having high amounts of a specific fatty acid are of immense importance for both nutritional and industrial purposes. Oil high in oleic acid has demand in commercial food-service applications due to a long shelf-life and cholesterol-reducing properties. Both linoleic and linolenic acids are essential fatty acids; however, less than 3% linolenic acid is preferred for oil stability. High erucic acid content is beneficial for the polymer industry, whereas low erucic acid is recommended for food purposes. Therefore, it is important to undertake systematic characterization of the available gene pool for its variable fatty acid profile to be utilized for specific purposes. In the present study the Indian rapeseed-mustard germplasm and some newly developed low-erucic-acid strains were analysed by GLC to study the fatty acid composition in these lines. The GLC analysis revealed that the rapeseed-mustard varieties being commonly grown in India are characterized by high erucic acid content (30–51%) in the oil with low levels of oleic acid (13–23%). However, from among the recently developed low-erucic-acid strains, several lines were identified with comparatively high oleic acid (60–70%), moderate to high linoleic acid (13–40%) and low linolenic acid (< 10%) contents. Work is in progress at TERI (New Delhi, India) to utilize these lines for development of strains with particular fatty acid compositions for specific purposes.

scholarly journals Development and Validation of Kompetitive Allele-Specific PCR Assays for Erucic Acid Content in Indian Mustard [Brassica juncea (L.) Czern and Coss.]

2021 ◽  
Vol 12 ◽  
Author(s):  
Karanjot Singh Gill ◽  
Gurpreet Kaur ◽  
Gurdeep Kaur ◽  
Jasmeet Kaur ◽  
Simarjeet Kaur Sra ◽  
...  
Keyword(s):  
Erucic Acid ◽  
Brassica Juncea ◽  
Erucic Acid Content ◽  
Oilseed Crop ◽  
High Erucic Acid ◽  
Specific Pcr ◽  
Allele Specific ◽  
Low Erucic Acid ◽  
Allele Specific Pcr ◽  
Kaspar Assays

Brassica juncea L. is the most widely cultivated oilseed crop in Indian subcontinent. Its seeds contain oil with very high concentration of erucic acid (≈50%). Of late, there is increasing emphasis on the development of low erucic acid varieties because of reported association of the consumption of high erucic acid oil with cardiac lipidosis. Erucic acid is synthesized from oleic acid by an elongation process involving two cycles of four sequential steps. Of which, the first step is catalyzed by β-ketoacyl-CoA synthase (KCS) encoded by the fatty acid elongase 1 (FAE1) gene in Brassica. Mutations in the coding region of the FAE1 lead to the loss of KCS activity and consequently a drastic reduction of erucic acid in the seeds. Molecular markers have been developed on the basis of variation available in the coding or promoter region(s) of the FAE1. However, majority of these markers are not breeder friendly and are rarely used in the breeding programs. Present studies were planned to develop robust kompetitive allele-specific PCR (KASPar) assays with high throughput and economics of scale. We first cloned and sequenced FAE1.1 and FAE1.2 from high and low erucic acid (&lt;2%) genotypes of B. juncea (AABB) and its progenitor species, B. rapa (AA) and B. nigra (BB). Sequence comparisons of FAE1.1 and FAE1.2 genes for low and high erucic acid genotypes revealed single nucleotide polymorphisms (SNPs) at 8 and 3 positions. Of these, three SNPs for FAE1.1 and one SNPs for FAE1.2 produced missense mutations, leading to amino acid modifications and inactivation of KCS enzyme. We used SNPs at positions 735 and 1,476 for genes FAE1.1 and FAE1.2, respectively, to develop KASPar assays. These markers were validated on a collection of diverse genotypes and a segregating backcross progeny. KASPar assays developed in this study will be useful for marker-assisted breeding, as these can track recessive alleles in their heterozygous state with high reproducibility.

Utilization of Rapeseed Oils of High and Low Erucic Acid Content

1974 ◽  
Vol 17 (3) ◽  
pp. 136-147 ◽  
Author(s):  
H. Vogtmann ◽  
D.R. Clandinin ◽  
R.T. Hardin
Keyword(s):  
Erucic Acid ◽  
Acid Content ◽  
Erucic Acid Content ◽  
Low Erucic Acid

Identification of Individual Brassica oleracea Plants with Low Erucic Acid Content

2004 ◽  
Vol 3 (5) ◽  
pp. 593-596 ◽  
Author(s):  
Fatih Seyis . ◽  
Wolfgang Friedt . ◽  
Axel Voss . ◽  
Wilfried Luhs .
Keyword(s):  
Erucic Acid ◽  
Brassica Oleracea ◽  
Acid Content ◽  
Erucic Acid Content ◽  
Low Erucic Acid

scholarly journals Nutritional and Antioxidant Properties of Rapeseed (Brassica Napus) Cultivars with High and Low Erucic Acid Content

2014 ◽  
Vol 2 (12) ◽  
pp. 918-924 ◽  
Author(s):  
Jin Yue ◽  
Pingping Shang ◽  
Gang Wang ◽  
Dan Liu ◽  
Ling Xu ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Erucic Acid ◽  
Acid Content ◽  
Antioxidant Properties ◽  
Erucic Acid Content ◽  
Low Erucic Acid

Inheritance of high oleic acid content in the seed oil of mutant Ethiopian mustard lines and its relationship with erucic acid content

2007 ◽  
Vol 145 (4) ◽  
pp. 353-365 ◽  
Author(s):  
M. DEL RÍO-CELESTINO ◽  
R. FONT ◽  
A. DE HARO-BAILÓN
Keyword(s):  
Oleic Acid ◽  
Erucic Acid ◽  
Acid Content ◽  
Seed Oil ◽  
Mutant Line ◽  
Chain Elongation ◽  
Erucic Acid Content ◽  
Interspecific Crosses ◽  
Oleic Acid Content ◽  
Ethiopian Mustard

SUMMARYEthiopian mustard (Brassica carinata) genotypes with different contents of oleic acid (C18:1) in the seed oil could be useful for food and industrial applications. The objectives of the present research were to study the inheritance of high C18:1 in the seed oil of different lines of Ethiopian mustard and its relationship with erucic acid content (C22:1). The low C18:1/high C22:1 mutant line L-1806, the high C18:1/high C22:1 mutant line L-482, the high C18:1/low C22:1 mutant line L-2890 and the low C18:1/very high C22:1 mutant line L-1630 were isolated after a chemical mutagen treatment of C-101 seeds (about 94 g C18:1/kg and 450 g C22:1/kg). The high C18:1/zero C22:1 line L-25X-1 was obtained by interspecific crosses of Ethiopian mustard with rapeseed and Indian mustard. Plants of lines L-2890×C-101, L-482×L-2890, L-1630×L-25X-1, L-1630×L-2890 and L-482×L-1806 were reciprocally crossed and F2 and the BC1F1 generations were obtained. Cytoplasmic effects were not observed in any of the crosses. The segregation pattern in F2 and BC1F1 populations differed in the crosses studied. The inheritance of C18:1 content in crosses segregating for this fatty acid was that expected for one (crosses between L-482×L-1806), two (L-2890×C-101) or three (L-1630×L-2890, L-1630×L-25X-1 and L-482×2890) loci. Oleic acid segregation indicated control of accumulation by two segregating genetic systems, one acting on chain elongation from C18:1 to C22:1 and the other involving desaturation from C18:1 to linoleic acid (C18:2). Accumulations of C18:1 and C22:1 were influenced by the same loci (M1, M2, E1 and E2), which control the chain elongation steps leading from C18:1 to C22:1. In addition, C18:1 was influenced by one additional locus (tentatively named OL) involved in control of desaturation of C18:1 to form C18:2. The genetic constitution of the parent lines would be OlOlE1E1E2E2m1m1m2m2 for L-2890, OlOlE1E1E2E2M1M1M2M2 for C-101, ololE1E1E2E2M1M1M2M2 for L-1630, OlOle1e1e2e2M1M1M2M2 for L-25X-1, ol1ol1E1E1E2E2M1M1M2M2 for L-482 and Ol1Ol1E1E1E2E2M1M1M2M2 for L-1806. Transgressive recombinants were obtained from the cross L-1630×L-25X-1, with about three-fold increase of the C18:1 content of the parents (>643 g/kg) and free of C22:1 content, which represent a high potential for commercial exploitation.

Increasing erucic acid content in Ethiopian mustard through mutation breeding

1998 ◽  
Vol 117 (1) ◽  
pp. 85-87 ◽  
Author(s):  
L. VELASCO ◽  
J. M. FERNÁNDEZ-MARTINEZ ◽  
A. de HARO
Keyword(s):  
Erucic Acid ◽  
Acid Content ◽  
Mutation Breeding ◽  
Erucic Acid Content ◽  
Ethiopian Mustard
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