scholarly journals Identification of RAPD markers linked to pod length in Brassica napus L. canola

2005 ◽  
Vol 85 (4) ◽  
pp. 803-808 ◽  
Author(s):  
Glen P. Hawkins ◽  
Xiugang Zhang ◽  
Mohan R. Thiagarjah ◽  
Lisa M. Corrigan ◽  
Gary R. Stringam
Keyword(s):  
Molecular Markers ◽  
Brassica Napus ◽  
Bulk Segregant Analysis ◽  
Rapd Markers ◽  
Total Yield ◽  
Additive Effects ◽  
Linkage Groups ◽  
Brassica Napus L ◽  
Spring Canola ◽  
Pod Length

The purpose of this study was to develop molecular markers as a tool to make important agronomic selections in the absence of a scorable phenotype. This project focused on the trait of pod length in Brassica napus L. canola. Pod length is an important parameter involved in total yield in this crop. In this study, the inheritance of long pods was found to be controlled by two independent genes in a dominant relationship with additive effects. Using bulk segregant analysis (BSA) and linkage analysis using RAPD markers, two corresponding linkage groups that segregate with the long pod trait were identified. The application of this trait for possible benefit in a canola breeding program is discussed. Key words: Spring canola, RAPD, pod length, plant breeding, Brassica napus

scholarly journals Interrelationship between yield and yield related traits of spring canola (Brassica napus L.) genotypes

Genetika ◽  
2007 ◽  
Vol 39 (3) ◽  
pp. 325-332 ◽  
Author(s):  
Sonja Ivanovska ◽  
Cane Stojkovski ◽  
Zoran Dimov ◽  
Ana Marjanovic-Jeromela ◽  
Mirjana Jankulovska ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Yield ◽  
Seed Weight ◽  
Coefficient Of Determination ◽  
Path Coefficient ◽  
Path Coefficient Analysis ◽  
Brassica Napus L ◽  
Plant Seed ◽  
Spring Canola ◽  
Pod Length

The research was conducted in order to determine yield related characters which are more effective as selection criteria than yield itself. Three spring canola (Brassica napus L.) genotypes have been investigated for plant height, number of primary branches (including the main raceme), number of pods per plant, pod length, number of seeds per pod, seed weight per pod and 1000 seed weight and their correlations with seed yield per plant. Seed yield had highest correlation with number of pods per plant (r= 0.935 and r= 0.973 in Skopje and Strumica, respectively), followed by seed weight per pod (r= 0.693 and r= 0.729) and 1000 seed weight (r= 0.627 and r= 0.680). Path coefficient analysis was used to identify the direct and indirect effect of studied characters on seed yield. Number of pods per plant (p=0.759 and p=0.877 for Skopje and Strumica, respectively) and seed weight per pod (p=0.216 and p=0.225) had the highest direct effect on seed yield in both locations followed by 1000 seed weight (p=0.056 and p=0.010). The coefficient of determination (R2) was 0.943.

scholarly journals Detection of self-incompatible oilseed rape plants (Brassica napus L.) based on molecular markers for identification of the class I S haplotype

2014 ◽  
Vol 37 (3) ◽  
pp. 556-559 ◽  
Author(s):  
Lenka Havlícková ◽  
Eva Jozová ◽  
Miroslav Klíma ◽  
Vratislav Kucera ◽  
Vladislav Curn
Keyword(s):  
Molecular Markers ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Class I ◽  
Brassica Napus L ◽  
S Haplotype

Potential biochemical, genetic and molecular markers of deterioration advancement in seeds of oilseed rape (Brassica napus L.)

2019 ◽  
Vol 130 ◽  
pp. 478-490 ◽  
Author(s):  
Justyna Boniecka ◽  
Karolina Kotowicz ◽  
Edyta Skrzypek ◽  
Kinga Dziurka ◽  
Monika Rewers ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Brassica Napus L ◽  
Biochemical Genetic

Re-evaluating sowing time of spring canola (Brassica napus L.) in south-eastern Australia—how early is too early?

2016 ◽  
Vol 67 (4) ◽  
pp. 381 ◽  
Author(s):  
J. A. Kirkegaard ◽  
J. M. Lilley ◽  
R. D. Brill ◽  
S. J. Sprague ◽  
N. A. Fettell ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oil Content ◽  
Yield Variability ◽  
Sensitivity Index ◽  
Brassica Napus L ◽  
South Eastern ◽  
Spring Canola ◽  
Sowing Dates ◽  
Eastern Australia ◽  
South Eastern Australia

Optimising the sowing date of canola (Brassica napus L.) in specific environments is an important determinant of yield worldwide. In eastern Australia, late April to early May has traditionally been considered the optimum sowing window for spring canola, with significant reduction in yield and oil in later sown crops. Recent and projected changes in climate, new vigorous hybrids, and improved fallow management and seeding equipment have stimulated a re-evaluation of early-April sowing to capture physiological advantages of greater biomass production and earlier flowering under contemporary conditions. Early–mid-April sowing generated the highest or equal highest yield and oil content in eight of nine field experiments conducted from 2002 to 2012 in south-eastern Australia. Declines in seed yield (–6.0% to –6.5%), oil content (–0.5% to –1.5%) and water-use efficiency (–3.8% to –5.5%) per week delay in sowing after early April reflected levels reported in previous studies with sowings from late April. Interactions with cultivar phenology were evident at some sites depending on seasonal conditions. There was no consistent difference in performance between hybrid and non-hybrid cultivars at the earliest sowing dates. Despite low temperatures thought to damage early pods at some sites (<−2°C), frost damage did not significantly compromise the yield of the early-sown crops, presumably because of greater impact of heat and water-stress in the later sown crops. A validated APSIM-Canola simulation study using 50 years of weather data at selected sites predicted highest potential yields from early-April sowing. However, the application of a frost-heat sensitivity index to account for impacts of temperature stress during the reproductive phase predicted lower yields and higher yield variability from early-April sowing. The frost–heat-limited yields predicted optimum sowing times of mid-April at southern sites, and late April to early May at the northern sites with lower median yield and higher yield variability in crops sown in early April. The experimental and simulation data are potentially compatible given that the experiments occurred during the decade of the Millennium drought in south-eastern Australia (2002–10), with dry and hot spring conditions favouring earlier sowing. However, the study reveals the need for more accurate and validated prediction of the frost and heat impacts on field-grown canola if simulation models are to provide more accurate prediction of attainable yield as new combinations of cultivar and sowing dates are explored.

scholarly journals Molecular Markers for the Scab Resistance (Vf) Region in Apple

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 855C-855 ◽  
Author(s):  
Minou Hemmat ◽  
Norman F. Weeden ◽  
Susan K. Brown
Keyword(s):  
Molecular Markers ◽  
Bulk Segregant Analysis ◽  
Rapd Markers ◽  
Venturia Inaequalis ◽  
Apple Scab ◽  
Genetic Resistance ◽  
Scab Resistance ◽  
Tight Linkage ◽  
Commercially Important ◽  
The Relationship

Apple scab, Venturia inaequalis (Cke.) Wint., is one of the most damaging diseases of apples. Although fungicide sprays have been used to control the disease, genetic resistance in existing commercially important varieties would be desirable. Identification of molecular marker(s) would be helpful in devising biotechnological approaches to control the disease. We used bulk segregant analysis to identify RAPD markers that cosegregate or display a tight linkage with Vf gene in Prima × Spartan cross. Using this approach, we are saturating the region around the scab resistance gene for the purpose of bracketing the locus. We have identified several markers associated with the Vf locus. The closest markers have been isolated and sequenced to be used as SCARs. The relationship and distances of the markers with the Vf locus and other previously reported markers will be discussed.

scholarly journals Genetic Diversity of Chinese and Japanese Rapeseed (Brassica napus L.) Varieties Detected by RAPD Markers.

2000 ◽  
Vol 50 (4) ◽  
pp. 257-265 ◽  
Author(s):  
Chaozhi Ma ◽  
Yusuke Kimura ◽  
Hideya Fujimoto ◽  
Takako Sakai ◽  
Jun Imamura ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Brassica Napus ◽  
Rapd Markers ◽  
Brassica Napus L

scholarly journals From Dimness to Glossiness—Characteristics of the Spring Rapeseed Mutant Form without Glaucous Bloom (Brassica napus L.)

Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1563
Author(s):  
Anna V. Shirokova ◽  
Valentina T. Volovik ◽  
Natalia V. Zagoskina ◽  
Georgiy P. Zaitsev ◽  
Hatima K. Khudyakova ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Shoot Growth ◽  
Lignin Content ◽  
Seedling Development ◽  
Mutant Form ◽  
Brassica Napus L ◽  
Spring Canola ◽  
Wax Coating ◽  
Dry Out

As a result of the treatment of “Vikros” spring canola with the chemical mutagen ethyl methanesulfonate (EMS), a high-protein mutant form without glaucous bloom (wax bloom) on leaves, shoots, and siliques was isolated. Segregation into glossy and glaucous forms was always observed in the progeny of glossy plants from self-pollination, and the proportion of glaucous plants could reach up to 25%. The progeny of glaucous plants were homogeneous and did not segregate. If during the period of seed germination and seedling development the soil did not dry out and remained moist, and the average daily temperature did not exceed 16 °C, then the amount of glossy plants could reach 99%. Glossy plants possessed qualities valuable for breeding forage varieties, such as the increased content of protein in seeds (more than 30%), and change phenol metabolism, чтo прoявляется a reduced amount of lignin and sinapine in comparison with the original cultivar. In addition, plants without wax coating showed weakened shoot growth, decreased pollen fertility and seed production, and reduced lignin content in the shoots. Glossy mutants are of interest for the obtaining of fodder low-sinapine and low-lignin varieties of spring rapeseed.

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