Effect of harvest period on the protein content in sea buckthorn leaves

2003 ◽  
Vol 83 (2) ◽  
pp. 409-410 ◽  
Author(s):  
T. S. C. Li ◽  
D. Wardle
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Freeze Drying ◽  
Conversion Factor ◽  
Growing Season ◽  
Sea Buckthorn ◽  
Leaf Protein ◽  
Total Protein Content ◽  
Male And Female ◽  
Freeze Dried

A study was conducted at Summerland, BC, in 2000 and 2001 to investigate the effect of harvest period on the protein content of sea buckthorn leaves. Leaves of both male and female plants were collected bi-weekly during the growing season and analyzed with a LECO FP-528 nitrogen analyzer after air- and freeze-drying. A conversion factor of kA = 5.7 was applied to obtain the protein content. Total protein content in sea buckthorn leaves showed very small differences between male and female plants and from either freeze-dried or air-dried materials. There were significant fluctuations among harvest periods in both years. Based on the results of this experiment, sea buckthorn leaves should be harvested from late July to early August since leaf protein content peaked around this period of time and started to decline significantly by the middle of August. Key words: Sea buckthorn, Hippophae rhamnoides L., leaf protein

Amino acid composition, protein content and accurate nitrogen-to-protein conversion factor for sheepgrass (Leymus chinensis)

Botany ◽  
2020 ◽  
Vol 98 (3) ◽  
pp. 137-146 ◽  
Author(s):  
Qingfen Zhang ◽  
Dongmei Qi ◽  
Xiaobing Dong ◽  
Xiaoxia Li ◽  
Liqin Cheng ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Protein Content ◽  
Acid Composition ◽  
Total Nitrogen ◽  
Total Protein ◽  
Conversion Factor ◽  
Leymus Chinensis ◽  
Total Protein Content ◽  
Protein Nitrogen

The protein content of plants is commonly estimated by multiplying the total nitrogen content (Kjeldahl; KN) by a nitrogen-to-protein conversion factor of 6.25. This method is based on the incorrect assumption that all nitrogen in the ammonia/ammonium and organic substances in plants is protein nitrogen, usually resulting in overestimation of protein content. We have examined amino acid composition, amino acid nitrogen, total nitrogen (KN), and actual protein content (AP) determined from amino acid residues in 16 accessions of perennial sheepgrass (Leymus chinensis (Trin.) Tzvelev). We determined a new nitrogen-to-protein conversion factor, kP, as the ratio of AP to KN, and applied this factor to estimate the total protein content (TP) as KN × kP. The non-protein nitrogen accounted for 40.5% to 62.4% of the total nitrogen. The average kP value was 3.17 overall, 3.20 in the accessions sampled at the jointing stage, and 3.15 in the accessions sampled at the flowering stage. The TP, calculated as KN × 3.17, was about half that of crude protein contents, calculated as KN × 6.25. Our study suggests that the AP-based kP of 3.17 can be used to more accurately estimate the total protein content in sheepgrass.

Prediction of Human Acute Toxicity by the Hep G2/24-hour/Total Protein Assay, with Protein Measurement by the CBQCA Method

2005 ◽  
Vol 33 (3) ◽  
pp. 207-213 ◽  
Author(s):  
Paul J. Dierickx
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Human Toxicity ◽  
Hep G2 ◽  
Vitro Assay ◽  
Protein Assay ◽  
Lowry Method ◽  
Total Protein Assay

In our previously described Hep G2/24-hour/total protein assay, protein levels were measured by using the Lowry method. This assay was the best acute in vitro assay for the prediction of human toxicity within the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) study. In order to increase the MEIC data-base with a wider range of chemicals, we were interested in introducing the more practical 3-(4-carboxybenzoyl)-quinoline-2-carboxaldehyde (CBQCA) method for the quantification of the total protein content. Therefore, we investigated whether the same good results for the prediction of acute human toxicity would be obtained with the CBQCA method. The cells were treated for 24 hours, then cytotoxicity was determined by measuring the total protein content with CBQCA. The results were quantified by using the PI50c: the concentration (in mM) of test compound required to reduce the total protein content measured with the CBQCA-method by 50% as compared to the control cells. The results were compared with the PI50, the corresponding value when the Lowry method was used. A relatively low correlation was observed between PI50 and PI50c, reflecting the large and unexpected, differences when using the two protein assays. However, when comparing the log PI50c with the human toxicity, a correlation coefficient of r2 = 0.761 ( n = 44) was obtained for exactly the same series of MEIC chemicals. This value is clearly higher than that for the Lowry method ( r2 = 0.695). Compared to the Lowry method originally used, the Hep G2/24-hour/CBQCA total protein assay has the additional important advantage that it can be very easily adapted for large-scale analyses with robotic systems, including the on-line calculation of the results.

Total protein content and protein synthesis within pre-elongation stage bovine embryos

1998 ◽  
Vol 50 (2) ◽  
pp. 139-145 ◽  
Author(s):  
J.G. Thompson ◽  
A.N.M. Sherman ◽  
N.W. Allen ◽  
L.T. McGowan ◽  
H.R. Tervit
Keyword(s):  
Protein Synthesis ◽  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Bovine Embryos

scholarly journals Surface topography of hydroxyapatite affects ROS17/2.8 cells response

2002 ◽  
Vol 16 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Adalberto Luiz Rosa ◽  
Márcio Mateus Beloti ◽  
Richard van Noort ◽  
Paul Vincent Hatton ◽  
Anne Jane Devlin
Keyword(s):  
Cell Proliferation ◽  
Protein Content ◽  
Surface Topography ◽  
Cell Morphology ◽  
Total Protein ◽  
Cell Attachment ◽  
Maxillofacial Surgery ◽  
Total Protein Content ◽  
Alp Activity ◽  
Powder Pressing

Hydroxyapatite (HA) has been used in orthopedic, dental, and maxillofacial surgery as a bone substitute. The aim of this investigation was to study the effect of surface topography produced by the presence of microporosity on cell response, evaluating: cell attachment, cell morphology, cell proliferation, total protein content, and alkaline phosphatase (ALP) activity. HA discs with different percentages of microporosity (< 5%, 15%, and 30%) were confected by means of the combination of uniaxial powder pressing and different sintering conditions. ROS17/2.8 cells were cultured on HA discs. For the evaluation of attachment, cells were cultured for two hours. Cell morphology was evaluated after seven days. After seven and fourteen days, cell proliferation, total protein content, and ALP activity were measured. Data were compared by means of ANOVA and Duncan’s multiple range test, when appropriate. Cell attachment (p = 0.11) and total protein content (p = 0.31) were not affected by surface topography. Proliferation after 7 and 14 days (p = 0.0007 and p = 0.003, respectively), and ALP activity (p = 0.0007) were both significantly decreased by the most irregular surface (HA30). These results suggest that initial cell events were not affected by surface topography, while surfaces with more regular topography, as those present in HA with 15% or less of microporosity, favored intermediary and final events such as cell proliferation and ALP activity.

Development and regression of exercise-induced cardiac hypertrophy in rats

1979 ◽  
Vol 236 (2) ◽  
pp. H268-H272 ◽  
Author(s):  
R. C. Hickson ◽  
G. T. Hammons ◽  
J. O. Holoszy
Keyword(s):  
Cytochrome C ◽  
Protein Content ◽  
Total Protein ◽  
Female Rats ◽  
Heart Weight ◽  
Total Protein Content ◽  
Mitochondrial Marker ◽  
Time Intervals ◽  
Control Value ◽  
Exercise Induced

Adult female rats were exercised by daily swimming. All the increase in heart weight induced by the exercise occurred within 14 days and averaged 30%. The half times of the increases in heart weight and total protein content were about 4.5 days, whereas that of cytochrome c, which was used as a mitochondrial marker, was 6.5 days. The total amounts of DNA and of hydroxyproline in the heart, which were used to evaluate the degree of connective tissue hyperplasia, increased only slightly (8% and 10%, respectively). Other animals were subjected to the same swimming program for 21 days. Groups of rats were killed at various time intervals after stopping exercise. Heart weight, total protein content, and total cytochrome c content decreased rapidly initially, with 60% of the total regression of hypertrophy occurring during the first week. Thereafter, heart weight fell more gradually toward the sedentary control value. The hydroxyproline content of the heart, which was increased 10%, did not decrease during the regression of the hypertrophy.

scholarly journals Amino Acid Composition of Milk from Cow, Sheep and Goat Raised in Ailano and Valle Agricola, Two Localities of ‘Alto Casertano’ (Campania Region)

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2431
Author(s):  
Nicola Landi ◽  
Sara Ragucci ◽  
Antimo Di Maro
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Protein Content ◽  
Total Protein ◽  
Free Amino ◽  
Raw Milk ◽  
Total Amino Acid ◽  
Total Protein Content ◽  
Campania Region

Cow, sheep and goat raw milk raised in Ailano and Valle Agricola territories (‘Alto Casertano’, Italy) were characterized (raw proteins, free and total amino acids content) to assess milk quality. Raw milk with the highest total protein content is sheep milk followed by goat and cow milk from both localities. Total amino acid content in cow, goat and sheep raw milk is 4.58, 4.81 and 6.62 g per 100 g, respectively, in which the most abundant amino acid is glutamic acid (~20.36 g per 100 g of proteins). Vice versa, the free amino acids content characteristic profiles are different for each species. In particular, the most abundant free amino acid in cow, sheep and goat raw milk is glutamic acid (9.07 mg per 100 g), tyrosine (4.72 mg per 100 g) and glycine (4.54 mg per 100 g), respectively. In addition, goat raw milk is a source of taurine (14.92 mg per 100 g), retrieved in low amount in cow (1.38 mg per 100 g) and sheep (2.10 mg per 100 g) raw milk. Overall, raw milk from ‘Alto Casertano’ show a high total protein content and are a good source of essential amino acids.

STUDYING THE INFLUENCE OF SEASONAL FACTORS ON CASEIN AND WHEY PROTEIN CONTENT IN RAW MILK AND CHEESE YIELD

2020 ◽  
pp. 142-151
Author(s):  
L. Bahdanava ◽  
A. Podryabinkina ◽  
I. Bahdanau ◽  
T. Savelyeva
Keyword(s):  
Protein Content ◽  
Whey Protein ◽  
Total Protein ◽  
Seasonal Changes ◽  
Whey Proteins ◽  
Raw Milk ◽  
National Average ◽  
Total Protein Content ◽  
Seasonal Factors

The article presents the results of research to study seasonal changes in the content of total protein, casein and whey proteins in raw milk and to analyze their impact on cheese yield. It was determined that the lowest casein content in raw milk (18% lower than the national average) was observed in October and March. The linear dependence of the cheese yield on both the total protein content and casein content was established.

Sign in / Sign up

Export Citation Format

Share Document