Bacillus cereus UW85 inoculation effects on growth, nodulation, and N accumulation in grain legumes: Controlled-environment studies

2002 ◽  
Vol 82 (2) ◽  
pp. 282-290 ◽  
Author(s):  
J. Kevin Vessey ◽  
Terry J. Buss
Keyword(s):  
Bacillus Cereus ◽  
Common Bean ◽  
Temperature Regime ◽  
Growth Promotion ◽  
Nitrogenase Activity ◽  
Controlled Environment ◽  
N Accumulation ◽  
Positive Effects ◽  
Whole Plant ◽  
Single Temperature

Treating seed with Bacillus cereus strain UW85 stimulates nodulation in soybean, but the underlying mechanisms of this stimulation are poorly understood. In this study we assessed the effects of inoculation on nodulation, nitrogenase activity and dry matter partitioning in soybean (cv. Maple Ridge), common bean (cv. OAC Rico) and pea (cv. Express) under controlled, gnotobiotic conditions. Plants were grown for 34 d under controlled-environment conditions without a mineral N source, at low two levels of (brady)rhizobia inoculation. Soybean and common bean were grown at a single temperature regime and pea was grown at two temperature regimes. Each trial consisted of five treatments (noninoculated control, low (brady)rhizobia inoculation, low (brady)rhizobia plus UW85 inoculation, high (brady)rhizobia inoculation, and high (brady)rhizobia plus UW85 inoculation) with six plants as replicates per treatment in a completely randomized design. Inoculation of soybean with UW85 increased growth of roots, shoots, and nodules across both levels of bradyrhizobia inoculation and increased plant N accumulation by 12%. In oculation with UW85 also increased whole-plant nodulation (nodules plant-1) by 16%, but there were no effects on specific nodulation [nodules g-1 root dry weight (DW)], individual nodule DW, nitrogenase activity (μmol H2 g-1 nodule DW h-1) or N2 fixation efficiency (g plant-N g-1 nodule DW). Inoculation of pea with UW85 at the low temperature regime increased whole-plant nodulation by 19%, but had no positive effects on any other growth parameters. Inoculation of common bean with UW85 had no positive effects on any aspects of growth, nodulation or nitrogenase activity. Our data indicate that under gnotobiotic conditions, UW85 inoculation increases nodulation in soybean indirectly by increasing root growth and not stimulating the nodulation process per se. The bacterium had little to no positive effects on pea and common bean symbioses. Key words: Bacillus cereus UW85, Glycine max, growth-promotion, nodulation, Phaseolus vulgaris, Pisum sativum

Bacillus cereus UW85 inoculation effects on growth, nodulation, and N accumulation in grain legumes: Field studies

2002 ◽  
Vol 82 (2) ◽  
pp. 291-298 ◽  
Author(s):  
W. John Bullied ◽  
Terry J. Buss ◽  
J. Kevin Vessey
Keyword(s):  
Bacillus Cereus ◽  
Common Bean ◽  
Growth Promotion ◽  
Nitrogenase Activity ◽  
Dry Weight ◽  
Field Studies ◽  
Grain Legumes ◽  
N Accumulation ◽  
N Content ◽  
Stimulation Of

Bacillus cereus strain UW85 was assessed for growth-promotion effects on soybean and common bean in the presence and absence of rhizobial inoculation at two field sites in Manitoba in 1994. Growth promotions due to B. cereus UW85 occurred for soybean only, and only at one site. Promotions in plant emergence in soybean were apparent at 60 d after planting (DAP), but stimulations in shoot dry weight (DW), N concentration, and N content were not apparent until 90 DAP. At maturity (120 DAP), inoculation with UW85 resulted in stimulation of seed yield by 9% and seed N content by 14%. However, stimulation in growth and N parameters by UW85 treatment was proportionally greater in the absence of B. japonicum inoculation than in the presence of the rhizobial inoculant. These observations, in combination with the observations that nitrogenase activity was not stimulated by UW85 treatment, clearly indicate that the UW85-mediated stimulation of growth and N accumulation of soybean is via a generalized stimulation of plant growth, and not via a stimulation in the soybean-B. japonicum symbiosis per se. Overall, our study indicates that inoculation with UW85 has the potential of increasing soybean production in western Canada, but these effects are site specific and are not seen in common bean. Key words: Bacillus cereus UW85, common bean, Glycine max, growth-promotion, Phaseolus vulgaris, soybean

The Effect of Strain of Rhizobium and of Temperature on Nodulation and Early Growth of Trifolium semipilosum

1986 ◽  
Vol 22 (2) ◽  
pp. 123-131 ◽  
Author(s):  
R. J. Roughley ◽  
R. A. Date
Keyword(s):  
Temperature Regime ◽  
Bacterial Strain ◽  
Early Growth ◽  
Nodule Formation ◽  
Controlled Environment ◽  
Root Temperature ◽  
Plant Yield ◽  
Nodule Number ◽  
Whole Plant ◽  
Time Required

SUMMARYThe effect of strain of Rhizobium and of temperature on nodule formation and early growth of Trifolium semipilosum was studied in controlled environment cabinets. Time required for nodules to form was affected by strain of Rhizobium and by root temperature, acting independently. Strains CB788 and CB2116 required only 9.5 to 10.0 days, averaging over temperatures, whereas strains CB782 and CB526 required 12.8 to 13.0 days. Nodulation was earliest at whole-plant temperatures of 25/20°C and 30/25°C day/night (average 9.5 days); 15 days were required at 20/15°C. Nodule number was independent of bacterial strain but dependent on temperature; nodules were fewest in the lowest and most in the highest temperature regime. Plant yield was determined by interactions between temperature and strain and was generally greatest at whole plant temperatures of 25/20°C.When nodulated plants growing at 25°C were transferred to a range of root temperatures, plants grew best at 20° and 25°C and with strains CB782 and CB2116.

scholarly journals Screening, Identification and Growth-Promotion Products of Multifunctional Bacteria in a Chinese Fir Plantation

Forests ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 120
Author(s):  
Guangyu Zhao ◽  
Yihui Wei ◽  
Jiaqi Chen ◽  
Yuhong Dong ◽  
Lingyu Hou ◽  
...  
Keyword(s):  
High Efficiency ◽  
Growth Promotion ◽  
Nitrogenase Activity ◽  
Acc Deaminase ◽  
Inorganic Phosphorus ◽  
Chinese Fir ◽  
Biochemical Tests ◽  
Growth Promoting ◽  
Chinese Fir Plantation ◽  
Physiological And Biochemical

Purpose: This research was aimed to screen and identify multifunctional phosphorus-dissolving bacteria of a Chinese fir (Cunninghamia lanceolata) plantation and study its phosphorus-dissolving characteristics in order to provide strain resources and a theoretical basis for developing the appropriate bacterial fertilizer of a Chinese fir plantation. Methods: First, phosphorus-dissolving bacteria were isolated from the woodland soil of a Chinese fir plantation by Pikovskava inorganic phosphorus medium (PVK). Then, some growth-promoting indicators of primary screening strains were determined, including the capacity of phosphorus-solubilized, nitrogenase activity, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, production of indole-3-acetic acid (IAA), secretion of iron carrier and so on. Finally, the screening multifunctional phosphorus-dissolving bacteria were identified, which were combined with colony characteristics, physiological and biochemical tests and molecular biotechnology. Results: (1) Thirteen phosphorus-dissolving bacteria were isolated and screened in total, and P5 (195.61 mg·L−1) had the strongest capacity of phosphorus-solubilized. Five phosphorus-dissolving bacteria were provided with nitrogenase activity, and the highest activity of nitrogenase was P10 and P5 (71.90 C2H4 nmol·mL−1·h−1 and 71.00 C2H4 nmol·mL−1·h−1, respectively). Four strains were provided with ACC deaminase activity, and the highest activity of ACC deaminase was P5 and P9, (0.74 μmol·mg−1·h−1 and 0.54 μmol·mg−1·h−1, respectively). Most strains could secrete IAA, and three strains of bacteria had a strong secretory ability, which could secrete IAA with a concentration greater than 15 mg·mL−1, and P5 was 18.00, P2 was 17.30, P6 was 15.59 (mg·mL−1). P5 produced carriers of iron better than others, and the ratio of the diameter of the iron production carrier ring to the diameter of the colony was 1.80, respectively, which was significantly higher than other strains. Combining all kinds of factors, P5 multifunctional phosphorus-dissolving bacteria were screened for eventual further study. (2) Strain P5 was identified as Burkholderia ubonensis, based on the colony characteristics, physiological and biochemical tests, 16SrDNA sequence analysis and phylogenetic tree construction. Conclusion: P5 has a variety of high-efficiency growth-promoting capabilities, and the ability to produce IAA, ACC deaminase activity and siderophore performance are significantly higher than other strains, which had great potential in the development of microbial fertilizer.

scholarly journals The Atmosphere’s Effect on Stainless Steel Slabs’ Oxide Formation in a CH4-Fuelled Reheating Furnace

Metals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 621
Author(s):  
Aleksi Laukka ◽  
Eetu-Pekka Heikkinen ◽  
Timo Fabritius
Keyword(s):  
Stainless Steel ◽  
Oxide Layer ◽  
Depth Profiling ◽  
Layer Growth ◽  
304 Stainless Steel ◽  
Oxide Growth ◽  
Breakaway Oxidation ◽  
Positive Effects ◽  
Steel Slab ◽  
Single Temperature

Utilising the oxyfuel practice for CH4-fuelled combustion has positive effects on the emissions, efficiency and cost of high temperature furnace practices. However, especially in older installations, oxyfuel usage requires retrofitting and alters the atmosphere in which the oxidation of the steel occurs, when compared to using air as the oxidiser. Stainless steel slab oxide growth during reheating was studied in different atmospheres. The simulated post-burn atmospheres from oxyfuel, lean oxyfuel and air-fuel practices were used to compare oxide-scale layer growth and morphology during simulated typical AISI 304 stainless steel slab reheating prior to hot rolling. Thermogravimetric measurements, glow discharge optical emission spectrometer (GDOES) and field-emission scanning electron microscope energy dispersive X-ray (FESEM-EDS) methodology were applied to discern differences between oxide growth and inner oxide layer morphology between the three practices. Switching from air to oxyfuel practice at a single temperature had the same increasing effect on the scale formation amount as a 25 °C temperature increase in air atmosphere. Inner oxide layer depth profiling revealed C, Si and Ni to be the main elements that differed between temperatures and atmospheres. A morphology study showed Si and Ni behaviour to be linked to breakaway oxidation.

scholarly journals The Effects of Plant Growth-Promoting Bacteria with Biostimulant Features on the Growth of a Local Onion Cultivar and a Commercial Zucchini Variety

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 888
Author(s):  
Giorgia Novello ◽  
Patrizia Cesaro ◽  
Elisa Bona ◽  
Nadia Massa ◽  
Fabio Gosetti ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Global Market ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Mineral Concentration ◽  
Positive Effects ◽  
Plant Growth Promoting ◽  
Onion Cultivar ◽  
Growth Promoting

The reduction of chemical inputs due to fertilizer and pesticide applications is a target shared both by farmers and consumers in order to minimize the side effects for human and environmental health. Among the possible strategies, the use of biostimulants has become increasingly important as demonstrated by the fast growth of their global market and by the increased rate of registration of new products. In this work, we assessed the effects of five bacterial strains (Pseudomonas fluorescens Pf4, P. putida S1Pf1, P. protegens Pf7, P. migulae 8R6, and Pseudomonas sp. 5Vm1K), which were chosen according to their previously reported plant growth promotion traits and their positive effects on fruit/seed nutrient contents, on a local onion cultivar and on zucchini. The possible variations induced by the inoculation with the bacterial strains on the onion nutritional components were also evaluated. Inoculation resulted in significant growth stimulation and improvement of the mineral concentration of the onion bulb, induced particularly by 5Vm1K and S1Pf1, and in different effects on the flowering of the zucchini plants according to the bacterial strain. The present study provides new information regarding the activity of the five plant growth-promoting bacteria (PGPB) strains on onion and zucchini, two plant species rarely considered by the scientific literature despite their economic relevance.

scholarly journals Comparative Genomics Provides Insights into the Taxonomy of Azoarcus and Reveals Separate Origins of Nif Genes in the Proposed Azoarcus and Aromatoleum Genera

Genes ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 71
Author(s):  
Roberto Tadeu Raittz ◽  
Camilla Reginatto De Pierri ◽  
Marta Maluk ◽  
Marcelo Bueno Batista ◽  
Manuel Carmona ◽  
...  
Keyword(s):  
Aromatic Compounds ◽  
Growth Promotion ◽  
Nitrogenase Activity ◽  
Full Length ◽  
Polluted Water ◽  
Nitrate Respiration ◽  
Nif Genes ◽  
Diverse Range ◽  
Single Strain ◽  
Group 2

Among other attributes, the Betaproteobacterial genus Azoarcus has biotechnological importance for plant growth-promotion and remediation of petroleum waste-polluted water and soils. It comprises at least two phylogenetically distinct groups. The “plant-associated” group includes strains that are isolated from the rhizosphere or root interior of the C4 plant Kallar Grass, but also strains from soil and/or water; all are considered to be obligate aerobes and all are diazotrophic. The other group (now partly incorporated into the new genus Aromatoleum) comprises a diverse range of species and strains that live in water or soil that is contaminated with petroleum and/or aromatic compounds; all are facultative or obligate anaerobes. Some are diazotrophs. A comparative genome analysis of 32 genomes from 30 Azoarcus-Aromatoleum strains was performed in order to delineate generic boundaries more precisely than the single gene, 16S rRNA, that has been commonly used in bacterial taxonomy. The origin of diazotrophy in Azoarcus-Aromatoleum was also investigated by comparing full-length sequences of nif genes, and by physiological measurements of nitrogenase activity using the acetylene reduction assay. Based on average nucleotide identity (ANI) and whole genome analyses, three major groups could be discerned: (i) Azoarcus comprising Az. communis, Az. indigens and Az. olearius, and two unnamed species complexes, (ii) Aromatoleum Group 1 comprising Ar. anaerobium, Ar. aromaticum, Ar. bremense, and Ar. buckelii, and (iii) Aromatoleum Group 2 comprising Ar. diolicum, Ar. evansii, Ar. petrolei, Ar. toluclasticum, Ar. tolulyticum, Ar. toluolicum, and Ar. toluvorans. Single strain lineages such as Azoarcus sp. KH32C, Az. pumilus, and Az. taiwanensis were also revealed. Full length sequences of nif-cluster genes revealed two groups of diazotrophs in Azoarcus-Aromatoleum with nif being derived from Dechloromonas in Azoarcus sensu stricto (and two Thauera strains) and from Azospira in Aromatoleum Group 2. Diazotrophy was confirmed in several strains, and for the first time in Az. communis LMG5514, Azoarcus sp. TTM-91 and Ar. toluolicum TT. In terms of ecology, with the exception of a few plant-associated strains in Azoarcus (s.s.), across the group, most strains/species are found in soil and water (often contaminated with petroleum or related aromatic compounds), sewage sludge, and seawater. The possession of nar, nap, nir, nor, and nos genes by most Azoarcus-Aromatoleum strains suggests that they have the potential to derive energy through anaerobic nitrate respiration, so this ability cannot be usefully used as a phenotypic marker to distinguish genera. However, the possession of bzd genes indicating the ability to degrade benzoate anaerobically plus the type of diazotrophy (aerobic vs. anaerobic) could, after confirmation of their functionality, be considered as distinguishing phenotypes in any new generic delineations. The taxonomy of the Azoarcus-Aromatoleum group should be revisited; retaining the generic name Azoarcus for its entirety, or creating additional genera are both possible outcomes.

Do sensory reviews make more sense? The mediation of objective perception in online review helpfulness

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Alberto Lopez ◽  
Ricardo Garza
Keyword(s):  
Purchase Intention ◽  
Product Performance ◽  
Mediation Effect ◽  
Controlled Environment ◽  
Content Type ◽  
Positive Effects ◽  
Sensory Marketing ◽  
Modeling Analysis ◽  
Overwhelming Evidence ◽  
Review Helpfulness

PurposeDo consumers rate reviews describing other consumers' sensory experience of a product (touch, smell, sight, hear and taste) as helpful or do they rate reviews describing more practical properties (product performance and characteristics/features) as more helpful? What is the effect of review helpfulness on purchase intention? Furthermore, why do consumers perceive sensory and non-sensory reviews differently? This study answers these questions.Design/methodology/approachThe authors analyze 447,792 Amazon reviews and perform a topic modeling analysis to extract the main topics that consumers express in their reviews. Then, the topics were used as regressors to predict the number of consumers who found the review helpful. Finally, a lab experiment was conducted to replicate the results in a more controlled environment to test the serial mediation effect.FindingsContrary to the overwhelming evidence supporting the positive effects of sensory elicitation in marketing, this study shows that sensory reviews are less likely to be helpful than non-sensory reviews. Moreover, a key reason why sensory reviews are less effective is that they decrease the objective perception of the review, a less objective review then decreases the level of helpfulness, which decreases purchase intention.Originality/valueThis study contributes to the interactive marketing field by investigating customer behavior and interactivity in online shopping sites and to the sensory marketing literature by identifying a boundary condition, the authors’ data suggest that sensory elicitations might not be processed positively by consumers when they are not directly experienced, but instead communicated by another consumer. Moreover, this study indicates how companies can encourage consumers to share more effective and helpful reviews.

scholarly journals Root colonization and growth promotion of soybean, wheat and Chinese cabbage by Bacillus cereus YL6

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0200181 ◽  
Author(s):  
Yongli Ku ◽  
Guoyi Xu ◽  
Xiaohong Tian ◽  
Huiqin Xie ◽  
Xiangna Yang ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Chinese Cabbage ◽  
Root Colonization ◽  
Growth Promotion

SELECTIVE CONTROL OF ANNUAL BLUEGRASS IN KENTUCKY BLUEGRASS TURF WITH LINURON

1979 ◽  
Vol 59 (2) ◽  
pp. 469-473 ◽  
Author(s):  
R. G. INGRATTA ◽  
G. R. STEPHENSON ◽  
C. M. SWITZER
Keyword(s):  
Temperature Regime ◽  
Poa Pratensis ◽  
Kentucky Bluegrass ◽  
Controlled Environment ◽  
Night Temperature ◽  
Annual Bluegrass ◽  
Selective Control ◽  
Excellent Control

Optimum top growth of annual bluegrass (Pao annua L.) and Kentucky bluegrass (Poa pratensis L.) was obtained at 24/12 °C day/night temperature regime in controlled environment studies. The tolerance of seedling Kentucky bluegrass to linuron [3-(3,4-dichlorophenyl)-1)methylurea] appeared to be greatest at this temperature regime when photoperiods were 16 h in length. A granular formulation of linuron gave excellent control of annual bluegrass in Kentucky bluegrass turf at 6.7 kg/ha when applied postemergence. At this rate, all culitivars of Kentucky bluegrass tested, with the exception of Fylking, were tolerant to linuron as a granular formulation. After application of linuron at 3.4–6.7 kg/ha, phytotoxic residues remained in the soil at sufficient levels to injure seedling Kentucky bluegrass for up to 3 mo.

scholarly journals New insight into how thigmomorphogenesis affects Epipremnum aureum plant development

2018 ◽  
Vol 36 (3) ◽  
pp. 330-340 ◽  
Author(s):  
Adalberto Di Benedetto ◽  
Claudio Galmarini ◽  
Jorge Tognetti
Keyword(s):  
Growth Promotion ◽  
Rooted Cuttings ◽  
Climbing Plants ◽  
Combination Treatments ◽  
Run Off ◽  
Whole Plant ◽  
Epipremnum Aureum ◽  
Negative Effect ◽  
Net Assimilation ◽  
Plant Level

ABSTRACT Climbing Epipremnum aureum plants develop larger leaves than unsupported, hanging plants. This effect may be regarded, in part, as a thigmomorphogenic response, but gravimorphogenetic effect may also be involved, since polar auxin transport is known to be negatively affected in plants with horizontal or hanging stems, which may result in an altered hormone balance at the whole plant level. The present work was aimed at studying how exogenous auxins and cytokinins may influence growth of E. aureum rooted cuttings under different training systems. Rooted cuttings of E. aureum were cultivated either climbing on an upright wooden board or creeping on the glasshouse bench or hanging from a basket. All leaves of each plant were sprayed to run-off at sunset with four indole-3-acetic acid (IAA) doses 7 days after transplanting and one week later, with four benzylaminopurine (BAP) concentrations, rendering 16 hormone combination treatments. The application of IAA or BAP at 50 mg L-1 to creeping and hanging plants significantly promoted growth but, in climbing plants, a negative effect was generally observed. Changes in net assimilation and photosynthetic rates, together with modified allometric coefficients, accounted for these responses. The higher growth promotion by exogenous growth regulators observed in creeping or hanging plants compared to climbing plants, may be interpreted mostly as a gravimorphogenetic response.

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