EFFECT OF EDTA AND DTPA ON AVAILABLE-P EXTRACTION WITH THE KELOWNA MULTIPLE ELEMENT EXTRACTANT

1989 ◽  
Vol 69 (2) ◽  
pp. 191-197
Author(s):  
W. VAN LIEROP
Keyword(s):  
Key Words ◽  
Reference Values ◽  
Calcareous Soils ◽  
Acid Soils ◽  
Available P ◽  
Complexing Agents ◽  
Simultaneous Extraction ◽  
Soil P ◽  
Regression Techniques

The objective of this study was to determine the effect of adding either 0.001M EDTA or 0.005M DTPA on the amount of P removed from acid and calcareous soils by the Kelowna and 0.25M HO Ac extractants. These complexing agents were studied for possible simultaneous extraction and determination of available Zn. To achieve that end, P-concentrations removed by these solutions from a group of acid, calcareous, and combined soils were compared against reference values obtained with 0.5M NaHCO3 (Olsen et al.) and the Kelowna extractant (0.25M HOAc + 0.015M NH4F) by means of graphing, correlation and regression techniques. Of the 80 soils studied, 40 were acid with pH (H2O) values ranging from 4.2 to 6.9 and the remainder having higher values up to 9.3. Results indicated that additions of either 0.001M EDTA or 0.005M DTPA to the Kelowna solution increased average extracted P concentrations by about 20 and 60%, respectively. Values removed by either of the new KEDTA and KDTPA solutions were closely related to those extracted with 0.5M NaHCO3 and Kelowna solutions on acid and calcareous soils (r values ≥ 0.96**). As EDTA and DTPA increased extracted soil P, these were added at 0.001 and 0.005M as NH4 preparations to 0.25M HOAc (AADTPA & AAEDTA; without fluoride), respectively, for determining whether these complexing agents could supplant F for P extraction. These solutions removed proportionally related amounts (r ≈ 0.94**) of P from calcareous, compared to the Kelowna and 0.5M NaHCO3 solutions, but relationships were less precise for acid soils (r ≈ 0.76**). These results suggest that the AADTPA or AAEDTA solution should be evaluated further before adoption for routine P determination in multiple element extractions. Key words: Mehlich in, acid soils, calcareous soils

scholarly journals Secretion of Gluconic Acid From Nguyenibacter sp. L1 Is Responsible for Solubilization of Aluminum Phosphate

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiao Li Li ◽  
Xue Qiang Zhao ◽  
Xiao Ying Dong ◽  
Jian Feng Ma ◽  
Ren Fang Shen
Keyword(s):  
Gluconic Acid ◽  
Calcareous Soils ◽  
Acid Soils ◽  
Aluminum Phosphate ◽  
Available P ◽  
Rrna Gene ◽  
Phosphate Solubilizing Bacteria ◽  
P Deficiency ◽  
Phosphate Solubilizing ◽  
Major Factors

Phosphorus (P) deficiency is one of the major factors limiting plant growth in acid soils, where most P is fixed by toxic aluminum (Al). Phosphate-solubilizing bacteria (PSBs) are important for the solubilization of fixed P in soils. Many PSBs have been isolated from neutral and calcareous soils, where calcium phosphate is the main P form, whereas PSBs in acid soils have received relatively little attention. In this study, we isolated a PSB strain from the rhizosphere of Lespedeza bicolor, a plant well adapted to acid soils. On the basis of its 16S rRNA gene sequence, this strain was identified as a Nguyenibacter species and named L1. After incubation of Nguyenibacter sp. L1 for 48 h in a culture medium containing AlPO4 as the sole P source, the concentration of available P increased from 10 to 225 mg L–1, and the pH decreased from 5.5 to 2.5. Nguyenibacter sp. L1 exhibited poor FePO4 solubilization ability. When the pH of non-PSB-inoculated medium was manually adjusted from 5.5 to 2.5, the concentration of available P only increased from 6 to 65 mg L–1, which indicates that growth medium acidification was not the main contributor to the solubilization of AlPO4 by Nguyenibacter sp. L1. In the presence of glucose, but not fructose, Nguyenibacter sp. L1 released large amounts of gluconic acid to solubilize AlPO4. Furthermore, external addition of gluconic acid enhanced AlPO4 solubilization and reduced Al toxicity to plants. We conclude that secretion of gluconic acid by Nguyenibacter sp. L1, which is dependent on glucose supply, is responsible for AlPO4 solubilization as well as the alleviation of Al phytotoxicity by this bacterial strain.

scholarly journals A Comparison of Methods for the Determination of Cation Exchange Capacity of Soils/Porównanie Metod Oznaczania Pojemności Wymiany Kationów I Sumy Kationów Wymiennych W Glebach

2014 ◽  
Vol 21 (3) ◽  
pp. 487-498 ◽  
Author(s):  
Dawid Jaremko ◽  
Dorota Kalembasa
Keyword(s):  
Organic Matter ◽  
Cation Exchange ◽  
Cation Exchange Capacity ◽  
Sodium Acetate ◽  
Calcareous Soils ◽  
Acid Soils ◽  
Barium Chloride ◽  
Exchange Capacity ◽  
Organic Carbon Content

Abstract The object of this study was to compare the results obtained with four methods of determination of cation exchange capacity (CEC) and sum of exchangeable cations (Ca, Mg, K) in soils. One of these methods is Kappen’s method and the others methods are based on different extracting reagents: sodium acetate (pH = 8.2), barium chloride and hexaamminecobalt(III) chloride. Values measured with barium ions and hexaamminecobalt(III) ions as index cations are very comparable and these two methods can be considered as equivalent. Kappen’s method gives overestimated results, especially for acid soils reach in organic matter and very calcareous soils. Sodium acetate, buffering the pH of the extracting solution, causes increase of numbers of negatively charged sites and particularly those bonded to organic matter and for this reason values obtained with this method are overestimated. Nevertheless, it is possible to correct this error for a given soil sample by regression equation considering pH of soil, clay and organic carbon content.

scholarly journals EXTRACTION OF POTASSIUM AND SODIUM FROM ACID AND CALCAREOUS SOILS WITH THE KELOWNA MULTIPLE ELEMENT EXTRACTANT

1989 ◽  
Vol 69 (2) ◽  
pp. 235-242 ◽  
Author(s):  
W. VAN LIEROP ◽  
N. A. GOUGH
Keyword(s):  
Calcareous Soils ◽  
Water Extract ◽  
Complexing Agents ◽  
Soil Test ◽  
Test Interpretation ◽  
Ph Values ◽  
Correlation Techniques ◽  
Regression Slopes ◽  
Soil Groups

The objective of this study was to evaluate the Kelowna multiple element extractant and some EDTA and DTPA modifications for simultaneous determination of potassium and sodium in acid and calcareous soils. To that end, the relationships between K and Na concentrations extracted with 1 N NH4OAc and the Kelowna multiple element extractant (0.25 N HOAc + 0.015 N NH4F) were assessed. However, relationships between K and Na concentrations removed by the modified Kelowna multiple element extractants containing either EDTA or DTPA (KEDTA & KDTPA with NH4F; and AAEDTA & AADTPA without NH4F) were evaluated by comparing values against those obtained with the Kelowna extractant. Addition of these complexing agents was of interest because of their potential in enabling simultaneous extraction of available Zn. The procedures were evaluated by contrasting K and Na concentrations extracted from 100 Canadian soils — half which had pH values between 4.1 and 6.9 (H2O) and a second group with values between 7 and 9.6 — by means of graphing, regression and correlation techniques. Potassium and sodium concentrations removed by the Kelowna extractant ranged from 25 to 510 μg and from 10 to 1420 μg mL−1 soil, respectively. The Kelowna and its EDTA and DTPA modifications extracted K and Na as effectively from acid as calcareous soils. This conclusion was supported by the similar regression slopes obtained when relating concentrations of K and Na removed from these soil groups by the multiple element extractants against those removed by 1 N NH4OAc (r-values ≥ 0.97**). However, the new multiple element extractants removed an average of about 20% less K than 1 N NH4OAc from these soils, though similar Na levels. The lower K levels removed from the Kelowna extractant, or by one of its modifications, may require that soil test interpretation norms be adjusted to compensate for the lower values when using one of these to determine K-availability. A significant correlation was found between extracted Na levels and 1:2 vol/vol water-extract conductivities (r ≥ 0.83**) suggesting that "higher" Na levels may be soluble instead of exchangeable. Some Na test interpretations are discussed in the text. Key words: EDTA, DTPA

DETERMINATION OF THE LIME REQUIREMENT FOR ACID SOILS IN ONTARIO USING THE SMP BUFFER METHODS

1986 ◽  
Vol 66 (2) ◽  
pp. 373-376 ◽  
Author(s):  
Y. K. SOON ◽  
T. E. BATES
Keyword(s):  
Key Words ◽  
Soil Acidity ◽  
Acid Soils ◽  
Quadratic Equation ◽  
Good Prediction ◽  
Mineral Soils

The accuracy of the Shoemaker-McLean-Pratt single buffer method and its double buffer variation was compared for determining the lime requirement of 41 acid mineral soils in Ontario. The single buffer method gave good prediction of the lime requirement when calibrated against Ca(OH)2-titrated soil acidity using a quadratic equation. Key words: Lime requirement, acid soils, SMP buffer

scholarly journals Phosphorus Sorption and Lime Requirements of Maize Growing Acid Soils of Kenya

2013 ◽  
Vol 2 (2) ◽  
pp. 116 ◽  
Author(s):  
P. O. Kisinyo ◽  
C. O. Othieno ◽  
S. O. Gudu ◽  
J. R. Okalebo ◽  
P. A. Opala ◽  
...  
Keyword(s):  
Acid Soils ◽  
Base Cations ◽  
Exchange Capacity ◽  
Available P ◽  
Available Phosphorus ◽  
Phosphorus Sorption ◽  
The West ◽  
Soil P ◽  
P Sorption ◽  
East And West

<p>In Kenya, maize (<em>Zea mays</em> L.) is mainly grown on acid soils in high rainfall areas. These soils are known for low available phosphorus (P), partly due to its sorption by aluminium (Al) and iron oxides. The study determined soil P sorption, lime requirements and the effects of lime on soil pH, Al levels and available P on the main maize growing acids soils in the highlands east and west of Rift Valley (RV), Kenya. Burnt lime containing 21% calcium oxide was used. The soils were strongly to extremely acid (pH 4.85-4.07), had high exchangeable Al<sup>3+</sup> (&gt; 2 cmol Al kg<sup>-1</sup>) and Al saturation (&gt; 20% Al), which most maize germplasm grown in Kenya are sensitive to. The base cations, cation exchange capacity and available P (&lt; 10 mg P kg<sup>-1</sup> bicarbonate extractable P) were low, except at one site in the highlands east of RV indicative with history of high fertilizer applications. Highlands east of RV soils had higher P sorption (343-402 mg P kg<sup>-1</sup>) than the west (107-258 mg P kg<sup>-1</sup>), probably because of their high Al<sup>3+ </sup>ions<sup> </sup>and also the energies of bonding between the soil colloids and phosphate ions. Highlands east of RV also had higher lime requirements (11.4-21.9 tons lime ha<sup>-1</sup>) than the west (5.3-9.8 tons lime ha<sup>-1</sup>). Due to differences in soil acidity, Al levels and P sorption capacities within and between highlands east and west of RV, blanket P fertilizer and lime recommendations may not serve all soils equally well.</p>

Evaluation on a Novel Phosphorus Fractionation Method in Acid Soils

2012 ◽  
Vol 204-208 ◽  
pp. 272-278
Author(s):  
Hong Jun Lei ◽  
Xin Liu ◽  
Bei Dou Xi ◽  
Duan Wei Zhu
Keyword(s):  
Soil Phosphorus ◽  
Acid Soils ◽  
Phosphorus Fractionation ◽  
Red Soil ◽  
Water Bath ◽  
Available P ◽  
Control Treatment ◽  
Available Phosphorus ◽  
Soil P ◽  
The Difference

Phosphorous fractionation is a method developed to estimate sizes of readily soil available P pool, soil P sub-pools and their ability to replenish the available P. Three types of acid soils (1aterite red soil, yellow red soil and brown red soil) were used in pot experiment under a rain-shelter condition to investigate the effect of lime amendment on P fractions and their bioavailability by plant of broad bean. A novel phosphorus fractionation scheme was developed and used to study the phosphorus fractionation of the tested soils compared with the two typical soil phosphorus fractionation schemes, adopting a series of extractants such as 0.25mol L-1 NaHCO3, 1h (for Ca2-P), 0.5mol L-1 NH4F (pH8.5), 1h (for Al-P), 0.7mol L-1 NaClO, pH 8.05, 85°C water bath 30min (for Org-P), 0.1mol L-1 NaOH-0.1Na2CO3, 4h (for Fe-P), 1mol L-1 NaOH, 85°C water bath 1h (for O-Al-P), 0.3 mol L-1 Na-citrate-0.5 g Na2S2O4 -0.5 mol L-1 NaOH, 85°C water bath 15min (for O-Fe-P), 0.25mol L-1 H2SO4, 1h (for Ca10-P). Main results are obtained just as follows: besides Ca2-P, Al-P, Fe-P and O-Fe-P are potentially available phosphorus resource. Although O-P reflects the difference of P between lime and control treatment well, when it appears as a whole, it needs further subdivision to reflect soil phosphorus biologically availability difference better.

scholarly journals Effectiveness of Direct Application of Phosphate Rock in Upland Acid Inceptisols Soils on Available-P and Maize Yield

2013 ◽  
Vol 18 (1) ◽  
pp. 1
Author(s):  
. Nurjaya ◽  
Dedi Nursyamsi
Keyword(s):  
Phosphate Rock ◽  
Critical Level ◽  
Block Design ◽  
Acid Soils ◽  
Maize Yield ◽  
Direct Application ◽  
Available P ◽  
Wet Season ◽  
Soil P ◽  
Soil Available P

Source of P fertilizer which is used by farmers in upland acid soils area is generally acidulated phosphate rock (PR), such as tripel super phosphate (TSP), super phosphate 36% P2O5 (SP-36), as well as partial acidulated phosphate rock (PAPR) which contain 10-30% P2O5. Their effectiveness, however, varies and depends on the soil and plant types. Phosphate rock fertilizers have a high prospects for acid soils because its effectiveness equals to the SP-36, cheaper, slow release, and its application can also leave  the residual P in the soil that available for plants for next few seasons. Field experiment aimed to study the effectiveness of direct application of PR at upland acid soils and its effect on soil available-P as well as maize (Zea mays L.) yield was conducted in Acid Inceptisols of Ciampea, Bogor in wet season years 2008/2009. The experiment was arranged by a Randomized Completely Block Design with 3 replications. Maize of P-12 variety was used as a plant indicator. The treatment consisted of 6 levels of phosphate rock: 0, 20, 30, 40, 50, and 60 kg P ha-1, as well as one level of SP-36 40 kg P ha-1 as standard fertilizer. In addition, urea of 300 kg ha-1 and KCl of 100 kg ha-1 were used as basal fertilization. The result showed that the application of PR in the amount ranging from 20 to 60 kg P ha-1 increased total-P and available-P, and pH, decreased exchangeable Al in the soils as well as increased maize straw and grain. Phosphate rock  application at 40 kg P ha-1 level was equally effective as SP-36 in the tested soils. Critical level of soil P for maize grown in the soil was 675 and 5.00 mg P2O5 kg-1 extracted with HCl 25% and Bray I, respectively. The requirement of P for maize grown in the soil to achieve maximum profit was 38 kg P ha-1 and 17.5 kg P  ha-1 or equivalent to PR of 583 and 268 kg ha-1 in low (soil P < critical level) and high (soil P > critical level) soil P status,  respectively.Keywords: Direct application, phosphate rock, soil available-P, upland acid soils.[How to Cite: Nurjaya and D Nursyamsi. 2013. Effectiveness of Direct Application of Phosphate Rock in Upland Acid Inceptisols Soils on Available-P and Maize Yield. J Trop Soils 18 (1): 1-9. doi: 10.5400/jts.2013.18.1.1] [Permalink/DOI: www.dx.doi.org/10.5400/jts.2013.18.1.1]  

DETERMINATION OF AFTER-RIPENING PERIOD AND HORMONAL RESPONSE OF OROBANCHE SOLMSII C.D. CLARKE SEEDS

1970 ◽  
Vol 11 (1) ◽  
Author(s):  
A. Bista ◽  
G. B. Khattri ◽  
B. D. Acharya ◽  
S. C. Srivastava
Keyword(s):  
Growth Hormone ◽  
Seed Germination ◽  
Key Words ◽  
Incubation Period ◽  
Seed Set ◽  
Summer Season ◽  
Conditioning Period ◽  
Root Parasite ◽  
One Year

To find out the ability of Orobanche seeds to germinate immediately after seed set, seeds were germinated periodically at an interval of three months for one year in GR24. Some Orobanche seeds were capable of germination immediately after seed set but most required about nine months as after ripening or incubation period to be able to germinate. The phenomenon of after ripening in Orobanche seeds could be taken as an ecological measure to dormant over following unfavorable wet summer season. The growth hormone studies on Orobanche seed germination have shown that GA3 at a concentration of 100 ppm substantially enhanced seed germination when applied during pre-conditioning period. NAA showed some stimulatory effect at 0.5 - 1.0 ppm when applied during post-conditioning period but the hormone if applied during pre-conditioning period inhibited the germination. Kinetin failed to stimulate the germination at all the concentrations tested. Key words: Germination, root-parasite, hormone. Ecoprint Vol.11(1) 2004.

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