CHANGES IN NATURAL 15N ABUNDANCE OF SOILS ASSOCIATED WITH TILLAGE PRACTICES
The objective of this study was to determine changes in N dynamics in an Orthic Black Chernozem as a result of two tillage practices (conventional and zero tillage) using the variations in the natural 15N abundance of different soil-N fractions. After 14 yr, no significant differences in isotope composition of total soil-N between the two tillage practices could be found. However, changes were detected in the natural 15N abundance of the acid-hydrolyzable N and various organo-mineral size fractions which led to useful comparisons of the nature of N under the two systems. The N-content of the hydrolyzable-N fraction was similar at the 0- to 4- and 8- to 16-cm depth under both tillage practices, while it was significantly different at the 4- to 8-cm depth. The δa15N of this fraction was consistently higher than that of total soil N at all depths only under zero tillage. This was associated with the presence of more labile N compounds under zero tillage. No differences in the isotopic composition of the organomineral size fractions were found at the 0- to 4-cm depth. At the 4- to 8- and 8- to 16-cm depths, the δa15N values of the finer particle size fractions were higher under zero tillage than under conventional tillage. This indicates a more labile nature of the N associated with these size fractions under zero tillage. Key words: δa15N, conventional tillage, zero tillage, total N, acid-hydrolyzable fraction, particle size fractions