scholarly journals EFFECTS OF VESICULAR-ARBUSCULAR MYCORRHIZA ON 14C AND 15N DISTRIBUTION IN NODULATED FABABEANS

1980 ◽  
Vol 60 (2) ◽  
pp. 241-250 ◽  
Author(s):  
P. C. PANG ◽  
E. A. PAUL

A two-compartment growth chamber in which the aboveground plant materials were exposed to 14CO2 and the belowground portion was exposed to 15N2 under normal atmospheric pressure was designed for carbon and nitrogen transfer studies. Vicia faba infected with vesicular-arbuscular fungus Glomus mossae and non-mycorrhizal plants fixed similar quantities of N2 at an age of 6½ wk. Approximately 0.10 mg N was fixed∙g−1 dry plant materials∙day−1 and 40 mg C∙g−1 dry matter day−1 were synthesized by mycorrhizal and non-mycorrhizal fababeans during 48 h exposure to 14CO2 at 6½ wk with no apparent difference in yield of dry matter. The non-mycorrhizal plants transferred 37% of the fixed 14C beneath ground. The mycorrhizal ones transferred 47% of the fixed 14C beneath ground. Most of the difference could be accounted for in the belowground respiration. The 14CO2 produced by root-microbial systems of the mycorrhizal fababeans was twice as great as that of the nonmycorrhizal; both contained active rhizobium.

1964 ◽  
Vol 15 (6) ◽  
pp. 974 ◽  
Author(s):  
N McCGraham

Balances of energy, carbon, and nitrogen were measured with fresh herbage cut at 28 days' regrowth, and with hay made from it. Measurements were made on four wether sheep at fasting and at four levels of feeding. The herbage contained paspalum grass and white clover in proportions ranging from 0.6 : 1 to 1.2 : 1, with dry matter between 11 and 17%. It contained (dry matter basis) : 10% ash, 3% soluble sugars, 25% cellulose, 23% crude protein, and 9% lignin; crude fibre was 22% and nitrogen-free extractives 42%. The hay had the same composition. Digestibility of organic matter was 75% for the fresh material and 73% for the hay; digestible energy values were 73 and 70% respectively. The difference between fresh herbage and hay was due entirely to a decrease in the digestibility of protein, from 82% to 73%, in drying. Efficiency of utilization of digested protein was not affected. Digestibility of both materials decreased by 2 to 4 units between the lowest and highest feeding levels. One other difference was found, viz. the ratio methane production/kcal digestible energy was 1–2% greater with the hay. Metabolizable energy was 82–84% of digestible energy for both materials. Heat production bore a linear relation to metabolizable energy above maintenance. Net availability of metabolizable energy was 75% between fasting and maintenance, and 52% above maintenance, for both the fresh and dry food. These quantities were predictable to within 8% from metabolizable energy or fibre digestibility; the normally used starch equivalent factors underestimated the correct values by some 25%.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 15-15
Author(s):  
Bradley J Heins ◽  
Siane C Luzzi ◽  
Robert D Gardner

Abstract The objective of this study was to evaluate the taste preference of calves fed Chlorella sp. microalgae produced from dairy lagoon wastewater. The study was conducted at the University of Minnesota West Central Research and Outreach Center, Morris, MN, dairy during May 2019. Six Holstein and crossbred dairy heifer calves were fed 0 (control), 30, and 60 g of Chlorella sp. daily in a sequential elimination study. For the 7-d experiment, day 1 to 2 were for diet adaptation and day 3 to 4 were for data collection. During the final 3 days, the primarily consumed treatment was removed to determine the second preferred treatment. The microalgae used in this study was isolated from the dairy wastewater lagoon. The microalgae biomass was produced using outdoor hanging bag bioreactors with Chlorella sp. to recycle the dairy wastewater. The biomass was sterilized and kept frozen at -4°C until fed to calves. Calves were housed individually in hutches with outdoor access under solar panels, with free-choice water. Kendall’s coefficient of concordance was calculated to rank the consumption of the treatments from most to least preferred using JMP 14.3 statistical software. Pairwise comparisons and Tukey adjustment were applied to evaluate the difference between the treatments for total intake. Calves consumed more (P < 0.05) dry matter from control grain (3.4 kg/d) compared to 30 g microalgae grain (2.42 kg/d) and 60 g microalgae grain (1.56 kg/d) during the first 2-d period. During the second 2-d (d 3 and 4) segment, dry matter intake was reduced (P < 0.05) for the 60 g microalgae treatment compared to the control and 30 g microalgae treatment. Five of six calves in this study always ranked the control treatment first (P < 0.05) when given a choice, and ranked the 30 g microalgae second choice. Results indicated that microalgae maybe added to calf starter grains without any adverse effects; however, calves preferred calf starter grains without microalgae.


2011 ◽  
Vol 52 (No. 3) ◽  
pp. 137-140 ◽  
Author(s):  
F. Nourbakhsh

Carbon and nitrogen transformations in soil are microbially mediated processes that are functionally related. The fate of C and N was monitored in a clay-textured soil (Typic Haplocambid) which was either unamended (control) or amended with various plant materials at the rate of 10 g residue C/kg soil. To evaluate C mineralization, soils were incubated for 46 days under aerobic conditions. Nitrogen mineralization/immobilization was evaluated at the end of eight-week incubation experiment. All CO<sub>2</sub> evolution data conformed well to a first-order kinetic model, C<sub>m&nbsp;</sub>= C<sub>0</sub> (1 &ndash; e<sup>&ndash;Kt</sup>). The product of K and C<sub>0 </sub>(KC<sub>0</sub>) was significantly correlated with some chemical and biochemical properties of the plant residues, including N concentration (r = 0.83, P &lt; 0.001), C:N (r = &ndash;0.64, P &lt; 0.05) and lignin:N (r = &ndash;0.81, P &lt; 0.001). Among the plant residue composition characteristics, N concentration (r = 0.96, P &lt; 0.001), C:N (r = &ndash;0.69, P &lt; 0.01) and lignin:N (r = &ndash;0.68, P &lt; 0.01) were significantly correlated with the net rates of N mineralization/immobilization (N<sub>m/i</sub>).


2013 ◽  
Vol 57 ◽  
pp. 341-348 ◽  
Author(s):  
S. Linnea Berglund ◽  
Göran I. Ågren ◽  
Alf Ekblad

1979 ◽  
Vol 93 (1) ◽  
pp. 115-120 ◽  
Author(s):  
A. A. Parsa ◽  
A. Wallace ◽  
J. P. Martin

SUMMARYIn a preliminary laboratory experiment in Iran, not reported here, 5 out of 30 plant materials incorporated into a highly calcareous soil had a positive effect on increasing the DTPA- (diethylenetriaminepentaacetic acid)-extractable Fe and all except one significantly increased the vegetative growth of sorghum. The plant materials included Lawsonia inermis L., Malva silvestris L., Zyzyphus nummularia Wak. and Lavandula carnopifolia L. A glasshouse study was repeated with two California soils pretreated with 0·5% Fe2O3 to determine if these organic materials have practical value in making Fe available to plants. A calcareous, Fe-deficient Hacienda (fine-loamy, mixed, thermic aquic natrargid) and a non-calcareous Yolo (fine-silty, mixed, non-acid, thermic typic xerothents). The previously mentioned organic materials and Laminaria saccharina L. (Lamour) were incorporated into the soils at two rates, 15000 and 20000μg/g, as air dry and in ash form. An adequate supply of major and micronutrients other than Fe was ensured. Other treatments included 5 μg Fe/g as FeSO4, Fe-138 chelate and control. All of the plant materials with the exception of L. carnopifolia significantly increased dry-matter yield and Fe, Zn, Cu and Mn uptake by sorghum in the Hacienda soil. In the Yolo soil the above were not significant. Thin-layer chromatography of the extracts of the plant materials revealed the presence of significant quantities of phenolic substances.


2014 ◽  
Vol 23 ◽  
pp. 47-62
Author(s):  
J. Philip Robinson ◽  
K. Nithya ◽  
R. Ramya ◽  
B. Karthikbalan ◽  
K. Kripa

Plant growth and physiological response of sesame (Sesamum indicum L.) were studied in controlled environment using normal soil and indigenous Vesicular-arbuscular mycorrhiza (VAM) fungi treated soil. The seedlings of Zea mays were inoculated with Giguspora species of VAM (Glomus fasiculatum) and the inoculum was multiplied with help of Zeamays seed bed. Sesame seeds were then inoculated into the bed and it was found that the plant height, shoots lengths, roots, biomass of shoot and roots were considerably increased in the mycorrhizal plants. The effect of VAM infection was assessed in pot experiment. In this comparative study, specific mycorrhizal fungi had consistent effects on various growth parameters such as the number of leaves, number of roots, shoot length, biomass of shoot and roots and biochemical parameters were observed at various time intervals by statistical analysis using two way ANOVA, it was confined with mycorrhizal and non-mycorrhizal infected plants. It was found that the ability of isolates to maintain the plant growth effectively in the case of mycorrhizal seedlings shows a maximum absorbtion of 0.77 ±0.2, shoot length is about 8.34 ±0.2, count of root and leaves are about 8.10 ±0.3, 5.6 ±0.3 respectively under mycorrhizal infection in 30days of analysis and had a positive effect on the growth at all intervals. Biochemical analysis were carried out to estimate the total chlorophyll, chrophyll A, chlorophyll B and Carotenoids contents and it was analyzed to be 9 ±0.5 mg/g, 8.3 ±0.5 mg/g, 3.6 ±0.5 mg/g, 4 ±0.3 mg/g respectively. At the 30th day of analysis for the mycorrhizal plants, it was found to be high in mycorrhizal seedlings which shows the symbiosis had improved the nutrient uptake of cultivated plants. Nevertheless G. fasiculatum was found to be the most efficient fungus and exhibited the highest levels of mycorrhizal colonization, as well as the greatest stimulation of physiological parameters.


2021 ◽  
Vol 26 (3) ◽  
pp. 444-449
Author(s):  
Ahmad Fanindi ◽  
Endang Sutedi ◽  
Harmini Harmini

Reproductive traits determine the selection method in plant breeding. The benggala grass of the Hamil cultivar was thought to be apomictic; thus, a study was conducted to determine its reproduction. The research began by studying the morphological characters, continued with observing the generative phase and seed production of the cultivar planted from seeds (generative) and from pols (vegetative). The experiment was conducted in the greenhouse of the Research Institute of Animal Production. The experimental design was a completely randomized design with ten replications, and the treatments were different types of plant materials: seeds and pols. The Hamil cultivar taken from RIAP Collection was planted in pots with a diameter of 40 cm and a height of 30 cm. The results showed that most of the morphological characters of the Hamil cultivar planted from seeds and from pols were not different (P >0.05), so it was presumed that they were apomictic. The difference in morphology was only in the length and width of the flag leaves and the length of the internodes, where the cultivars planted using seeds were higher. The production of seeds, pithy seed weight, and seed germination of cultivars planted using seeds were higher (P <0.05). Further research is needed to determine the apomixis properties based on cytologically Hamil cultivars.   Keywords: apomixis, benggala grass, morphology, plnting material; seed


2019 ◽  
Vol 40 (6Supl2) ◽  
pp. 3157
Author(s):  
Simone Pedro da Silva ◽  
Carina Ubirajara de Faria ◽  
Ana Caroline Rodrigues da Cunha ◽  
Aline Maria Soares Ferreira ◽  
Jean Marcos Castro Paula ◽  
...  

We aimed to evaluate the effects of using a concentrated ration containing industrialised human food on the productive parameters of Nellore heifers in confinement, in addition to comparing the dry matter intake (DMI) observed with that predicted by the nutritional systems BR-Corte and NRC, in order to determine which system makes the most accurate predictions for heifers of the breed Nelore in feedlots. Twenty-four Nelore heifers, with an average age of 24 months and average body weight (BW) of 350 kg, were used over the 70 days of the experiment. They were randomly assigned to two treatments and housed in partially covered stalls equipped with an automatic Growsafe® Systems feeding system. Animals in the control group (CONT) received a concentrated ration containing ground corn, soybean meal, urea, and mineral nucleus. In the residual treatment (RES) group, heifers received concentrated feed containing products from the human diet. The difference between the treatments was the concentrated ration; in the control treatment, the energy source used was maize, whereas in the residue treatment, the energy sources were corn chips, coconut candies, and ground coffee. Corn silage was included as forage for both treatments. The forage:concentrate (F:C) ratio was 84:16. The predicted intake was estimated according to the nutritional systems NRC (2000, 2016) and BR-Corte (VALADARES FILHO et al., 2010, 2016). The average DMI observed of the heifers was 7.46 kg day-1. Heifers fed with human feed residue had a higher dry matter intake (DMI) and higher ether extract intake (EEI) than animals fed with the control feed. In addition, heifers that received human feed products showed a lower average daily weight gain (DWG) of 1.08 kg day-1 than animals fed the control treatment with an average DWG of 1.24 kg day-1. The predicted dry matter intake by NRC 2000 was an overestimate by 9.78 % and by BR-Corte 2010 was an overestimate by 5.5 %. The NRC 2016 and BR-Corte 2016 underestimated the intake of heifers by 18.9 % and 7.1 %, respectively. It was concluded that growing Nelore heifers fed with human feed products, with a F:C ratio of 84:16, present a higher DMI and lower growth performance and feed efficiency than heifers fed the control diet. The DMI predicting models for Nellore heifers proposed by BR-Corte are more adequate than the NRC models.


1987 ◽  
Vol 70 (3) ◽  
pp. 428-433
Author(s):  
Denis R Lauren ◽  
Donald E McNaughton ◽  
Michael P Agnew

Abstract A dichloromethane extraction system has been developed which is suitable for alfalfa carotenoids and is compatible with nonaqueous reverse phase liquid chromatographic (LC) analysis. Ground samples are extracted in the presence of antioxidant with dichloromethaneacetone (2 + 1). After an overnight soak, the mixture is saponified with 40% w/v potassium hydroxide in methanol and then is partitioned with 10% w/v aqueous sodium sulfate. An aliquot of the lower yellow organic phase is used for LC analysis after dilution (1 + 1) with acetonitrile. An isocratic 2 solvent system of ethyl acetateacetonitrile containing 0.1% n-decanol as mobile phase modifier is used for liquid chromatography with a high carbon loading non-endcapped ODS packing material. In comparisons with the existing AOAC method for carotenes and xanthophylls in dried plant materials and mixed feeds, the proposed method gave consistently higher results in a range of 7-28%, depending on the substrate. Possible causes for the differences were examined and include losses during partition and column chromatography with the AOAC method. Calculation methods and the choice of standard materials may also contribute to the difference in results.


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