ALFALFA DISEASE IN SOME ALBERTA SOILS

1976 ◽  
Vol 56 (2) ◽  
pp. 97-103 ◽  
Author(s):  
SALIH M. DAMIRGI ◽  
F. D. COOK ◽  
G. R. WEBSTER

Attempts were made to evaluate the potential biological factor causing poor growth (stunting) of alfalfa (Medicago sativa L.) on some soils in Central Alberta. Paratylenchus projectus which associates with stunted alfalfa was extracted from an affected soil. Alfalfa seedlings grown in sterilized sand culture assemblies were inoculated with P. projectus alone and in combination with dilutions from sterilized and non-sterilized affected and normal soils. Other studies included the effects of various sterilized and non-sterilized soil dilutions from an affected soil on plant growth and incidence of alfalfa stunting symptoms. Results indicate that P. projectus is not the biological factor causing alfalfa stunting. Typical stunting symptoms were observed on plants receiving non-sterilized soil dilutions from an affected soil, and the consistency of development of irregular reddish-brown root lesions on stunted plant roots confirmed the presence of other biological factor(s) causing the disease. It was reasonably well established that the root rot disease was caused by a microorganism(s). Further studies regarding root lesioning, isolation and identification of the microorganisms are being carried on in this laboratory.

2020 ◽  
Vol 8 (4) ◽  
pp. 496
Author(s):  
Dilfuza Egamberdieva ◽  
Vyacheslav Shurigin ◽  
Burak Alaylar ◽  
Hua Ma ◽  
Marina E. H. Müller ◽  
...  

The effects of biochar on plant growth vary depending on the applied biochar type, study site environmental conditions, microbial species, and plant–microbial interactions. The objectives of the present study were therefore to assess 1) the response of growth parameters of lupin and root disease incidence to the application of three biochar types in a loamy sandy soil, and 2) the role of endophytic bacteria in biological control of root rot disease incidence in lupin after the amendment of soil with different biochar types. As biochar types we tested (i) hydrochar (HTC) from maize silage, (ii) pyrolysis char from maize (MBC), and (iii) pyrolysis char from wood (WBC) at three different concentrations (1%, 2%, and 3% of char as soil amendments). There were no significant effects in lupin shoot and root growth in soils amended with WBC at any of the concentrations. MBC did not affect plant growth except for root dry weight at 2% MBC. HTC char at 2% concentration, significantly increased the root dry weight of lupin by 54–75%, and shoot dry weight by 21–25%. Lupin plants grown in soil amended with 2% and 3% WBC and MBC chars showed 40–50% and 10–20% disease symptoms, respectively. Plants grown in soil without biochar and with HTC char were healthy, and no disease incidence occurred. Pseudomonas putida L2 and Stenotrophomonas pavanii L8 isolates demonstrated a disease reduction compared to un-inoculated plants under MBC and WBC amended soil that was infested with Fusarium solani.


Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 696
Author(s):  
Mona M. Khaleil ◽  
Maryam M. Alnoman ◽  
Elsayed S. Abd Elrazik ◽  
Hayat Zagloul ◽  
Ahmed Mohamed Aly Khalil

Fusarium solani, the causative agent of root rot disease is one of the major constraints of faba bean (Vicia faba L.) yield worldwide. Essential oils have become excellent plant growth stimulators besides their antifungal properties. Foeniculum vulgare Mill. (fennel) is a familiar medicinal plant that has inhibitory effects against phytopathogenic fungi. Herein, different concentrations of fennel seed essential oil (FSEO) (12.5, 25, 50, 100, 200 and 400 μL/mL) were examined against F. solani KHA10 (accession number MW444555) isolated from rotted roots of faba bean in vitro and in vivo. The chemical composition of FSEO, through gas chromatography/mass spectroscopy, revealed 10 major compounds. In vitro, FSEO inhibited F. solani with a minimum inhibitory concentration (MIC) of 25 µL/mL. In vivo, FSEO suppressed Fusarium root rot disease in Vicia faba L. by decreasing the disease severity (61.2%) and disease incidence (50%), and acted as protective agent (32.5%) of Vicia faba L. Improvements in morphological and biochemical parameters were recorded in FSEO-treated faba seeds. Moreover, the expression level of the defense-related genes defensin and chitinase was noticeably enhanced in treated plants. This study suggested using FSEO as a promising antifungal agent against F. solani not only to control root rot disease but also to enhance plant growth and activate plant defense.


HortScience ◽  
2004 ◽  
Vol 39 (6) ◽  
pp. 1339-1342
Author(s):  
Todd L. Mervosh ◽  
James A. LaMondia

The effects of terbacil herbicide on strawberry (Fragaria ×ananassa Duch. `Honeoye') yield and black root rot disease were determined in field plots at two locations in Connecticut over 4 years. Terbacil treatments at up to four times the maximum label dosage caused some temporary foliar chlorosis but did not affect the health of structural or perennial roots and associated feeder roots. Development of secondary root growth (perennial roots) was not influenced by terbacil. Terbacil had no effect on the quantity of lesion nematodes [Pratylenchus penetrans (Cobb) Filip & Schur. Stek.] extracted or the amount of the fungal pathogen Rhizoctonia fragariae Husain and McKeen isolated from strawberry roots. At both locations, R. fragariae was common on plant roots by the fourth year. Terbacil treatments did not affect strawberry yields in terms of number or weight of ripe berries per plot. Our results indicate that terbacil does not contribute to black root rot or decreased yields in `Honeoye' strawberry. Chemical name used: 5-chloro-3-(1,1-dimethylethyl)-6-methyl-2,4-(1H,3H)-pyrimidinedione (terbacil).


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