CHROMATOGRAPHIC FRACTIONATION OF ORGANIC PHOSPHATES FROM ALKALI, ACID, AND AQUEOUS ACETYLACETONE EXTRACTS OF SOILS

1970 ◽  
Vol 50 (2) ◽  
pp. 111-119 ◽  
Author(s):  
R. L. HALSTEAD ◽  
G. ANDERSON
Keyword(s):  
Acid Hydrolysis ◽  
Anion Exchange ◽  
Paper Chromatography ◽  
Inositol Phosphates ◽  
Organic P ◽  
P Fractions ◽  
Chromatographic Fractionation ◽  
Anion Exchange Resins ◽  
Canadian Soils ◽  
Exchange Resins

Extraction with hot 3 N NaOH removed more total organic P, and inositol P fractions, from Scottish and Canadian soils than did extraction with hot 6 N HCl, hot concentrated HCl, or 0.2 M aqueous acetylacetone at pH 8. Only a relatively small amount of the organic P in the acetylacetone extracts could be adsorbed by anion exchange resins unless the extracts were first subjected to hydrolysis with NaOH. This in one case led to a fivefold increase in the phosphate adsorbed and subsequently eluted with NH4 formate. The proportion of higher inositol phosphates (penta- and hexa-) to the lower esters was greater in the Scottish than the Canadian soils. Paper chromatography revealed that compounds with Rf values similar to glycerophosphate and glucose-1-P were present in some fractions. Gas and paper chromatography of the fractions after acid hydrolysis revealed compounds with retention times and Rf values corresponding to glycerol and ribitol, but their identity was not confirmed. The neo-, DL-, scyllo- and myo-isomers of the inositol were identified in fractions containing lower inositol phosphates (iP2, iP3, iP4) and in those containing the higher esters.

Separation of the nitrogenous products of the acid hydrolysis of proteins on anion exchange resins

1980 ◽  
Vol 14 (4) ◽  
pp. 247-250
Author(s):  
Z. A. Chaplygina ◽  
N. A. Gorskaya ◽  
Z. S. Tkhorzhevskaya
Keyword(s):  
Acid Hydrolysis ◽  
Anion Exchange ◽  
Anion Exchange Resins ◽  
Exchange Resins ◽  
Hydrolysis Of

scholarly journals Automated isocratic high-performance liquid chromatography of inositol phosphate isomers

1989 ◽  
Vol 262 (3) ◽  
pp. 997-1000 ◽  
Author(s):  
K A Wreggett ◽  
R F Irvine
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Anion Exchange ◽  
High Performance ◽  
Inositol Phosphates ◽  
Inositol Phosphate ◽  
Direct Analysis ◽  
Isocratic Elution ◽  
Anion Exchange Resins ◽  
Exchange Resins

Isomers of inositol phosphates from biological samples can be analysed by anion-exchange h.p.l.c., by using isocratic elution with phosphate buffers. The method involves the preliminary processing of the extracted samples with conventional soft-gel anion-exchange resins, including the commonly used Dowex resins, followed by direct analysis with h.p.l.c. of a portion of relevant fractions. Run times (up to 20 min) and collected fraction numbers (up to 24) are minimal, so that if the method is used in conjunction with automated h.p.l.c. injection a high throughput of samples is achieved.

Removal of Hydrophobic and Hydrophilic Constituents by Anion Exchange Resin

1999 ◽  
Vol 40 (9) ◽  
pp. 207-214 ◽  
Author(s):  
J.-P. Croué ◽  
D. Violleau ◽  
C. Bodaire ◽  
B. Legube
Keyword(s):  
Molecular Weight ◽  
Anion Exchange ◽  
Water Source ◽  
Atomic Ratio ◽  
Size Exclusion ◽  
Salting Out ◽  
Anion Exchange Resins ◽  
Hydrophilic Character ◽  
Exchange Resins

The objective of this work was to compare the affinity of well characterized NOM fractions isolated from two surface waters with strong (gel matrix and macroporous matrix) and weak anion exchange resins (AER) using batch experiment conditions. The structural characterization of the fraction of NOM has shown that the higher the hydrophilic character, the lower the C/O atomic ratio, the lower the SUVA, the lower the aromatic carbon content and the lower the molecular weight. In general (not always), strong AER was more efficient to remove DOC than weak AER. For the same water source (Suwannee River), the higher the molecular weight of the NOM fraction, the lower the affinity with AER. Increasing the ionic strength favored the removal of the hydrophobic NOM fraction (“salting out” effect) while increasing the pH apparently reduced the removal of the hydrophilic NOM fraction. Results were discussed in terms of size exclusion, adsorption, anion exchange and also hydrophobic/hydrophilic repulsion.

scholarly journals Impact of anion exchange adsorbents on regional citrate anticoagulation

2020 ◽  
pp. 039139882094773
Author(s):  
Karin Strobl ◽  
Stephan Harm ◽  
Ute Fichtinger ◽  
Claudia Schildböck ◽  
Jens Hartmann
Keyword(s):  
Anion Exchange ◽  
Regional Citrate Anticoagulation ◽  
Liver Support ◽  
Anion Exchange Resins ◽  
Increased Risk ◽  
Extracorporeal Liver Support ◽  
Target Molecules ◽  
Exchange Resins ◽  
The Impact

Introduction: Heparin and citrate are commonly used anticoagulants in membrane/adsorption based extracorporeal liver support systems. However, anion exchange resins employed for the removal of negatively charged target molecules including bilirubin may also deplete these anticoagulants due to their negative charge. The aim of this study was to evaluate the adsorption of citrate by anion exchange resins and the impact on extracorporeal Ca2+ concentrations. Methods: Liver support treatments were simulated in vitro. Citrate and Ca2+ concentrations were measured pre and post albumin filter as well as pre and post adsorbents. In addition, batch experiments were performed to quantify citrate adsorption. Results: Pre albumin filter target Ca2+ concentrations were reached well with only minor deviations. Citrate was adsorbed by anion exchange resins, resulting in a higher Ca2+ concentration downstream of the adsorbent cartridges during the first hour of treatment. Conclusions: The anion exchange resin depletes citrate, leading to an increased Ca2+ concentration in the extracorporeal circuit, which may cause an increased risk of clotting during the first hour of treatment. An increase of citrate infusion during the first hour of treatment should therefore be considered to compensate for the adsorption of citrate.

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