FREE AMINO ACID COMPOSITION OF THE PLANT ROOT ENVIRONMENT UNDER FIELD CONDITIONS

1969 ◽  
Vol 49 (1) ◽  
pp. 121-127 ◽  
Author(s):  
K. C. Ivarson ◽  
F. J. Sowden
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino ◽  
Plant Root ◽  
Extraction Procedure ◽  
Amino Acid Content ◽  
Field Conditions ◽  
Natural Conditions ◽  
Root Extracts ◽  
Free Amino Acid Composition

The free amino acids and the total ninhydrin-reacting material found in the rhizosphere of plants grown under field conditions were compared with those in the nonrhizosphere soil and with root extracts. Water, 20% ethanol and carbon tetrachloride-water were used as extractants. While the last two removed more amino acids than the water, this was probably the result of partial decomposition of the cellular material of the soil. The use of water as an extractant should give a better measure of the amount of amino acids in the soil under natural conditions. The rhizosphere contained greater quantities of amino acids than the soil from outside this zone. In general, the most prominent amino acids were aspartic acid, threonine, serine, glutamic acid, glycine and alanine; asparagine, glutamine and citrulline were also present. The amino acid content of the rhizosphere varied with the plant. Although root extracts contained very large quantities of amino acids, comparison of these extracts with the rhizosphere showed distinct differences in amino acid ratios, suggesting that the amino acids in the rhizosphere were not primarily the result of root-debris autolysis during the extraction procedure.

Evaluation of seed amino acid content and its correlation network analysis in wild soybean (Glycine soja) germplasm in Japan

2021 ◽  
Vol 19 (1) ◽  
pp. 35-43
Author(s):  
Awatsaya Chotekajorn ◽  
Takuyu Hashiguchi ◽  
Masatsugu Hashiguchi ◽  
Hidenori Tanaka ◽  
Ryo Akashi
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Acid Content ◽  
Free Amino ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Amino Acid Content ◽  
Soybean Seeds ◽  
Free Amino Acid Content

AbstractWild soybean (Glycine soja) is a valuable genetic resource for soybean improvement. Seed composition profiles provide beneficial information for the effective conservation and utilization of wild soybeans. Therefore, this study aimed to assess the variation in free amino acid abundance in the seeds of wild soybean germplasm collected in Japan. The free amino acid content in the seeds from 316 accessions of wild soybean ranged from 0.965 to 5.987 mg/g seed dry weight (DW), representing a 6.2-fold difference. Three amino acids had the highest coefficient of variation (CV): asparagine (1.15), histidine (0.95) and glutamine (0.94). Arginine (0.775 mg/g DW) was the predominant amino acid in wild soybean seeds, whereas the least abundant seed amino acid was glutamine (0.008 mg/g DW). A correlation network revealed significant positive relationships among most amino acids. Wild soybean seeds from different regions of origin had significantly different levels of several amino acids. In addition, a significant correlation between latitude and longitude of the collection sites and the total free amino acid content of seeds was observed. Our study reports diverse phenotypic data on the free amino acid content in seeds of wild soybean resources collected from throughout Japan. This information will be useful in conservation programmes for Japanese wild soybean and for the selection of accessions with favourable characteristics in future legume crop improvement efforts.

THE METABOLISM OF YEAST SPORULATION: VI. CHANGES IN AMINO ACID CONTENT DURING SPOROGENESIS

1964 ◽  
Vol 10 (5) ◽  
pp. 623-631 ◽  
Author(s):  
C. Ramirez ◽  
J. J. Miller
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Content ◽  
Proline Content ◽  
Sporulation Medium ◽  
Free Proline

During 6-day exposures of cells of Saccharomyces cerevisiae to acetate sporulation medium, the content of free amino acids declined to approximately one-third of that of vegetative cells, but proline was exceptional in that it increased conspicuously in amount. The content of combined amino acids also diminished to about one-third, ammonia was evolved, and amino acids (not including proline) passed out of the cells into the medium. When dihydroxyacetone replaced acetate in the sporulation medium, the results were similar except that the decline in content of free and combined amino acids was much greater, more ammonia was evolved, and only very small amounts of amino acids could be detected in the medium. Transfer of sporulated cells to growth medium led to an increase in the pool of free amino acids, except for proline, which declined in amount.In two other species of Saccharomyces the free proline content also increased on exposure to sporulation medium, but in Schizosaccharomyces pombe and Torulopsis famata no such increase was observed.

Total amino acid requirements of weaned piglets 8 to 25 kg live weight given diets based on wheat and soya-bean meal fortified with free amino acids

1992 ◽  
Vol 54 (2) ◽  
pp. 281-287 ◽  
Author(s):  
F. Gatel ◽  
G. Buron ◽  
J. Fékéte
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino ◽  
Amino Acid Content ◽  
Live Weight ◽  
Maximum Growth ◽  
Soya Bean ◽  
Weaned Piglets ◽  
Bean Meal ◽  
Soya Bean Meal

AbstractTwo experiments were carried out with weaned piglets from 8 to 25 kg live weight in order to determine the dietary amino acid content necessary for maximum growth. Six diets based on wheat, soya-bean meal, soya-bean oil and free amino acids were compared in each experiment. Essential amino acids were in the same relative proportion for all diets: (methionine + cystine)/lysine = 0·60 to 0·65; threonine/lysine = 0·65; tryptophan/lysine = 0·19. The range of amino acid content was 9·53 to 12·52 g lysine per kg in the first experiment and 11·34 to 15·94 g lysine per kg in the second experiment. The number of piglets used per diet was 136 (20 pens) and 106 (16 pens) in respectively the first and the second experiment. The relationship between either dietary lysine content or daily lysine intake and growth rate was quadratic and significant. Dietary lysine content and daily lysine intake which enable maximum growth were calculated according to this model. Dietary lysine contents were 15·5 and 14·9 g/kg for the first 3 weeks (8 to 17 kg) and for the overall post-weaning period (8 to 25 kg) respectively. Daily lysine intakes were 10·6 and 13·3 g/day respectively for the same two periods. Reasons for these values being higher than those currently cited are discussed.

Free Amino Acids in Human Saliva

1973 ◽  
Vol 52 (3) ◽  
pp. 418-419 ◽  
Author(s):  
Pan H. Papanayotou ◽  
Joanna Dozi-Vassiliades ◽  
Anast. Kovatsis
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Thin Layer ◽  
Column Chromatography ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Content ◽  
Human Saliva ◽  
Chromatographic Techniques

By the use of thin layer chromatographic techniques and exchange column chromatography, 20 free amino acids were found in human saliva. No qualitative or semiquantitative differences in the amino acid content were observed among caries-susceptible and caries-resistant individuals 20 to 30 years old.

THE VENOM OF THE MUD-DAUBER WASPS: I. SCELIPHRON CAEMENTARIUM: PRELIMINARY SEPARATIONS AND FREE AMINO ACID CONTENT

1963 ◽  
Vol 41 (1) ◽  
pp. 1943-1948 ◽  
Author(s):  
Rod O'Connor ◽  
Wm. Rosenbrook Jr.
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Thin Layer ◽  
Acid Content ◽  
Free Amino ◽  
Amino Acid Content ◽  
Electrical Excitation ◽  
Free Amino Acid Content ◽  
Quantitative Investigation ◽  
Layer Chromatography

Quantitative investigation of Sceliphron caementarium venom, obtained by electrical excitation of the wasp, has shown an unexpectedly small protein fraction of the dried venom. A few percent of the venom consists of the free amino acids histidine, methionine, and pipecolic acid. Three other components have been isolated by paper and thin-layer chromatography and are found to give weak ninhydrin reactions, in addition to certain color tests characteristic of alkaloids.

scholarly journals Free Amino Acids and Biogenic Amines in Canned European Eels: Influence of Processing Step, Filling Medium and Storage Time

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1377
Author(s):  
Lucía Gómez-Limia ◽  
Roxana Cutillas ◽  
Javier Carballo ◽  
Inmaculada Franco ◽  
Sidonia Martínez
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Biogenic Amines ◽  
Free Amino Acid ◽  
Biogenic Amine ◽  
Free Amino Acids ◽  
Free Amino ◽  
Storage Time ◽  
Amino Acid Content ◽  
And Storage

This study evaluated the effects of the canning process and different filling media on the free amino acid and biogenic amine contents of eels. The main free amino acids were histidine, taurine and arginine, which constituted 72% of the free amino acids in raw eels. All steps in the canning process significantly altered the free amino acid content of eels, relative to raw samples. The changes were influenced by the step, the composition of the frying or filling medium and the storage time. The biogenic amine contents were very low in all samples. Histamine was not detected in either raw eels or canned eels. The highest values were obtained for 2-phenylethylamine. The step of the canning process, the composition of the frying or filling medium and storage time also determined the changes in the biogenic amine contents. The biogenic amines indices were low, indicating the good quality of canned eels.

INFLUENCE DE LA FERTILISATION AZOTEE, PHOSPHATEE ET POTASSIQUE SUR L’INCORPORATION DES ACIDES AMINES AUX PROTEINES ET LES RENDEMENTS DE LA LUZERNE

1972 ◽  
Vol 52 (1) ◽  
pp. 41-52 ◽  
Author(s):  
S. T. NGUYEN ◽  
L. J. O’GRADY ◽  
G. J. OUELLETTE ◽  
R. PAQUIN
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Free Amino ◽  
Amino Acid Content ◽  
Free Amino Acid Content ◽  
Acides Aminés ◽  
Phosphorus And Potassium ◽  
Nitrogen Phosphorus ◽  
Fertilisation Azotée

A well-balanced fertilizer with nitrogen, phosphorus, and potassium decreases the free amino acid content of alfalfa, increases the incorporation of these amino acids into proteins, and betters the yield of that legume. Potassium appears to be required for the incorporation of amino acids into proteins, and potassium interaction with phosphorus should be taken into consideration to obtain maximum incorporation and yield. The influence of fertilizers on some of the most important amino acids in alfalfa is discussed.

scholarly journals Free amino acids as phagostimulants in cricket nuptial gifts: support for the ‘Candymaker’ hypothesis

2009 ◽  
Vol 5 (2) ◽  
pp. 194-196 ◽  
Author(s):  
Stuart Warwick ◽  
Karim Vahed ◽  
David Raubenheimer ◽  
Stephen J Simpson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
High Performance ◽  
Amino Acid Content ◽  
Free Amino Acid Content ◽  
Nuptial Gifts

Nuptial gifts that are manufactured by the male are found in numerous insect species and some spiders, but there have been very few studies of the composition of such gifts. If, as has been proposed recently, nuptial gifts represent sensory traps, males will be selected to produce gifts that are attractive to females but such gifts will not necessarily provide the female with nutritional benefits (the ‘Candymaker’ hypothesis). We examined the free amino acid content of the spermatophylax of the cricket Gryllodes sigillatus (Orthoptera: Gryllidae) using high performance liquid chromatography (HPLC). The spermatophylax (dry weight) consisted of approximately 7 per cent free amino acids. The free amino acid composition was highly imbalanced, with a low proportion of essential amino acids (18.7%) and a high proportion of proline and glycine. The main free amino acids found in the spermatophylax appeared to act as phagostimulants: the duration of feeding on artificial gels by females was positively related to the free amino acid content of the gels. The results therefore suggest that males use free amino acids to ‘sweeten’ a relatively low-value food item. A possible function of glycine in inhibiting female movement is also proposed.

ENDOCRINE CONTROL OF FREE AMINO ACIDS CONTENT IN UTERINE FLUID IN PREGNANT RABBITS

1972 ◽  
Vol 70 (2) ◽  
pp. 409-416 ◽  
Author(s):  
S. Jaszczak ◽  
E. S. E. Hafez
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Blood Serum ◽  
Aspartic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Content ◽  
Endocrine Control ◽  
Uterine Fluid

ABSTRACT Free amino acid content was measured in the uterine fluid and blood serum in the following groups of rabbits 168 h post copulation: intact; intact progesterone-treated; ovariectomized progesterone-treated; ovariectomized progesterone-oestradiol-treated; and ovariectomized without hormonal treatment. At implantation, concentration of the majority of amino acid in uterine fluid exceeded greatly that of blood serum; the difference in concentration being maximal for glycine, taurine, alanine, glutamic acid, aspartic acid, serine and threonine. Glutamine-asparagine and arginine were found in comparable quantities or were even higher in blood serum. Glycine, alanine, taurine, glutamic acid, serine and glutamine-asparagine were found in highest concentration in the uterine fluid. The level of ammonia in uterine fluid was also relatively high. Exogenous progesterone and oestradiol caused significant changes in the concentration of some amino acid. The concentration of glycine, taurine, alanine, serine, glutamic acid, aspartic acid, threonine, ½ cystine and histidine seems to be especially hormonally dependent. The results are discussed in relation to hormonal activity of corpora lutea during early pregnancy, physiological significance of free amino acids in uterine fluid and nourishment of an early embryo.

Free amino acids of sunflower hypocotyl tissue during crown gall tumor development

1968 ◽  
Vol 46 (3) ◽  
pp. 217-223 ◽  
Author(s):  
R. M. Behki ◽  
S. M. Lesley
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Content ◽  
Total Content ◽  
Free Proline ◽  
Total Free Amino Acids ◽  
Hypocotyl Tissue

The concentration of each free amino acid present in hypocotyl tissue of sunflower seedlings was determined during the period following both sterile wounding and inoculation with either tumorigenic (strain 1025) or non-tumorigenic (strain IIBNV6) Agrobacterium tumefaciens. The concentration of the total free amino acids of hypocotyl tissue reached a maximum 68 hours after infection with strain 1025 and this level was 50% greater than that found in plants treated with strain IIBNV6. The total content of free basic amino acids increased to a peak value at 20 hours after infection by strain 1025 and this represented 1.4 to 3 times the level found in strain IIBNV6 inoculated seedlings at 20 hours.The amount of free proline decreased with time and it was essentially absent 164 hours after infection with strain 1025. At 2 months the concentration of free proline in the tumor tissue was greatly increased with 75% of the total free amino acids accounted for as proline.The free amino acid content of strain 1025 growing in either the presence or absence of an extract from wounded seedlings was measured and the results indicated no significant relationship between the plant and bacterial systems with regard to their free amino acid pools.

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