The influence of Fusarium oxysporum f. sp. medicaginis on total soluble-sugar concentration of infected alfalfa roots

1993 ◽  
Vol 73 (2) ◽  
pp. 647-649 ◽  
Author(s):  
C. Richard ◽  
J.-G. Martin
Keyword(s):  
Fusarium Oxysporum ◽  
Key Words ◽  
Freezing Tolerance ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Frost Tolerance ◽  
Root Tissue ◽  
Sugar Concentration ◽  
Low Concentration ◽  
Total Soluble Sugar

Alfalfa was inoculated with Fusarium oxysporum f. sp. medicaginis, and the total soluble-sugar concentration was determined in diseased taproots of cold-hardened plants. The concentration of soluble sugars decreased with increasing discoloration of root tissue (r = −0.85, P ≤ 0.0001). The low concentration of soluble sugars might be responsible for the previously reported low freezing tolerance of Fusarium-infected alfalfa. Key words: Alfalfa, Fusarium, sugars, frost tolerance

scholarly journals Effects of Leachate Fertigation and the Addition of Hydrogen Peroxide on Growth and Nutrient Balance in Dracaena deremensis Potted Plants

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 127
Author(s):  
Pedro García-Caparrós ◽  
Cristina Velasquez Espino ◽  
María Teresa Lao
Keyword(s):  
Nutrient Solution ◽  
Soluble Sugar ◽  
Nutrient Balance ◽  
Treatment Plant ◽  
Dry Weight ◽  
Sugar Concentration ◽  
Control Treatment ◽  
Peat Moss ◽  
Clear Trend ◽  
Total Soluble Sugar

The reuse of drainages for cultivating more salt tolerant crops can be a useful tool especially in arid regions, where there are severe problems for crops water management. Dracaena deremensis L. plants were cultured in pots with sphagnum peat-moss and were subjected to three fertigation treatments for 8 weeks: control treatment or standard nutrient solution (D0), raw leachates from Chrysalidocarpus lutescens H. Wendl plants (DL) and the same leachate blending with H2O2 (1.2 M) at 1% (v/v) (DL + H2O2). After harvesting, ornamental and biomass parameters, leaf and root proline and total soluble sugar concentration and nutrient balance were assessed in each fertigation treatment. Plant height, leaf and total dry weight had the highest values in plants fertigated with leachates with H2O2, whereas root length, leaf number, RGB values and pigment concentration declined significantly in plants fertigated with leachates from C. lutescens with or without H2O2. The fertigation with leachates, regardless of the presence or absence of H2O2 increased root and leaf proline concentration. Nevertheless, root and leaf total soluble sugar concentration did not show a clear trend under the treatments assessed. Regarding nutrient balance, the addition of H2O2 in the leachate resulted in an increase in plant nutrient uptake and efficiency compared to the control treatment. The fertigation with leachates with or without H2O2 increased nitrogen and potassium leached per plant compared to plants fertigated with the standard nutrient solution. The reuse of drainages is a viable option to produce ornamental plants reducing the problematic associated with the water consumption and the release of nutrients into the environment.

Total Soluble Sugar Quantification from Ethanolic Plant Extracts v1

2021 ◽  
Author(s):  
Lynn Doran ◽  
Amanda P. De Souza
Keyword(s):  
Plant Extracts ◽  
Plant Tissue ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Total Soluble Sugars ◽  
Total Soluble Sugar ◽  
Tissue Extracts

Quantification of total soluble sugars (as glucose) in plant tissue extracts via the sulfuric phenol method adapted for 96 well plates.

scholarly journals Invertase and sucrose synthase activities in coffee plants sprayed with sucrose solution

2003 ◽  
Vol 60 (2) ◽  
pp. 239-244 ◽  
Author(s):  
José Carlos da Silva ◽  
José Donizeti Alves ◽  
Amauri Alves de Alvarenga ◽  
Marcelo Murad Magalhães ◽  
Dárlan Einstein do Livramento ◽  
...  
Keyword(s):  
Sucrose Synthase ◽  
Sucrose Solution ◽  
Management Practice ◽  
Physiological State ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Total Soluble Sugar ◽  
Coffee Plants ◽  
Sugar Levels ◽  
High Level

One management practice of which the efficiency has not yet been scientifically tested is spraying coffee plants with diluted sucrose solutions as a source of carbon for the plant. This paper evaluates the effect of foliar spraying with sugar on the endogenous level of carbohydrates and on the activities of invertase and sucrose synthase in coffee (Coffea arabica L.) seedlings with reduced (low) and high (normal) levels of carbon reserve. The concentrations used were 0.5 and 1.0% sucrose, and water as a control. The use of sucrose at 1.0% caused an increase in the concentration of total soluble sugars in depauperate plants, as well as increased the activity of the following enzymes: cell wall and vacuole acid invertase, neutral cytosol invertase and sucrose synthase. In plants with high level of carbon reserve, no increments in total soluble sugar levels or in enzymatic activity were observed. Regardless of treatments or plants physiological state, no differences in transpiration or stomatal conductance were observed, demonstrating the stomatal control of transpiration. Photosynthesis was stimulated with the use of 0.5 and 1.0 % sucrose only in depauperate plants. Coffee seedling spraying with sucrose is only efficient for depauperate plants, at the concentration of 1.0%.

scholarly journals Effects of static magnetic field on growth, some biochemical and antioxidant system in lemon balm (Melissa officinalis L.) seedlings

2021 ◽  
Vol 20 (1) ◽  
pp. 71-80
Author(s):  
Anousheh Zakeri ◽  
Ramazan-Ali Khavari-Nejad ◽  
Sara Saadatmand ◽  
Fatemeh Nouri Kootanaee ◽  
Rouzbeh Abbaszadeh
Keyword(s):  
Magnetic Field ◽  
Phenolic Compounds ◽  
Static Magnetic Field ◽  
Plant Biomass ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Antioxidant Defense System ◽  
Total Soluble Sugars ◽  
Lemon Balm ◽  
Total Soluble Sugar

The effects of magnetic waves as natural environmental factors on the Earths are not well known on plant growth and development. The present study was carried out to evaluate the effects of static magnetic field (SMF) treatment (4 and 6 mT for 30 and 120 min per day) for eight days on the biomass production, proline contents and total soluble sugar, phenolic compounds, accumulation of H2O2 and MDA along with activity of antioxidant enzymes in lemon balm seedlings. Our results showed that SMF treatments, especially 6 mT and 120 min duration, increased the plant biomass, proline contents, phenolic compounds, H2O2 and MDA accumulation, and reduced the contents of total soluble sugars. The SMF application also increased the activity of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) enzymes compared to untreated seedlings. Our results suggest that SMF treatments induces the antioxidant defense system in the lemon balm seedlings and, by changing the plant metabolism, improves the early vigor of seedlings.

scholarly journals Studies on the Sugar Accumulation and Carbohydrate Splitting Enzyme Levels in Post Harvested and Cold Stored Potatoes

1970 ◽  
Vol 16 ◽  
pp. 95-99 ◽  
Author(s):  
Md Rezaul Karim ◽  
MMH Khan ◽  
Md Salim Uddin ◽  
NK Sana ◽  
F Nikkon ◽  
...  
Keyword(s):  
Key Words ◽  
Cold Storage ◽  
Enzyme Activities ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Reducing Sugar ◽  
Sugar Accumulation ◽  
Potato Varieties ◽  
Total Soluble Sugar

Significant differences were found in sugar content and carbohydrate splitting enzyme activities in tubers of ten indigenous potato varieties at harvesting and after keeping at cold storage. The activities of invertase, amylase, β-galactosidase and cellulase in all varieties were found to be increased by 2-12, 1.2-4, 1.9-4.5, and 1.1-3.7 folds, respectively from harvesting to cold stored potatoes. The amount of starch and sucrose were found to be decreased by 1.15-2.8 and 1.02-1.4 folds, respectively from harvesting to cold stored in all varieties. Total soluble sugar and reducing sugar contents in potatoes were increased by 1.02-1.4 and 4-11 folds, respectively from harvesting to cold stored in all varieties of potatoes. The amount of reducing sugar increased in cold stored potatoes due to the increased activities of carbohydrate splitting enzymes. Key words: Potatoes, Indigenous, Carbohydrate splitting enzymes, Sugars. DOI:10.3329/jbs.v16i0.3748 J. bio-sci. 16: 95-99, 2008

scholarly journals Biochemical and phytochemical evaluation of Stevia rebaudiana

2016 ◽  
Vol 2 (1) ◽  
pp. 121-130 ◽  
Author(s):  
Md Moniruzzaman Sohag Howlader ◽  
Sheikh Rashel Ahmed ◽  
Khadizatul Kubra ◽  
Md Khairul Hassan Bhuiyan
Keyword(s):  
Phenolic Compounds ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Stevia Rebaudiana ◽  
Reducing Sugar ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Dry Extract ◽  
Total Soluble Sugar

The present study was aimed to evaluate biochemical and phytotochemical of dry leaves of Stevia rebaudiana. Biochemical analysis indicated that Stevia leaves are a good source of carbohydrate and reducing sugar. Different extraction methods were used to prepare four different dry extracts (Extract A, B, C, D). Total soluble sugars and reducing sugars were analyzed for these four dry extracts and commercial Stevia powder. The highest amount of total soluble sugar (477 mg sugar g-1 dry extract) was obtained from extract C and higher amount of reducing sugar (82 mg g-1 dry extract) was obtained from extract D among the extracts. But commercial Stevia powder showed higher total soluble sugar content (754 mg g-1 dry powder) and highest amount of reducing sugar (98 mg g-1 dry extract) than all the extracted dry samples. The extraction process of dry extract C was feasible for the extraction of total soluble sugar. For the phytochemical screening, crude extract was tested for the presence of different chemical groups and presence of alkaloids, phenolic compounds, steroids, tannins, flavonoids, cardiac glycosides and saponins that were identified. The highest amount of total phenolic compounds (92 mg) was recorded from methanolic extract of extraction B. The lowest amount of total phenolic compounds (36 mg) was recorded in ethanolic extract of extraction A. So, Methanol proved as best solvent to extract increased quantity of total phenolic compounds than other solvents.Asian J. Med. Biol. Res. March 2016, 2(1): 121-130

scholarly journals Regulation of curcuminoids, photosynthetic abilities, total soluble sugar, and rhizome yield traits in two cultivars of turmeric (Curcuma longa) using exogenous foliar paclobutrazol

2021 ◽  
Vol 49 (3) ◽  
pp. 12445
Author(s):  
Daonapa CHUNGLOO ◽  
Rujira TISARUM ◽  
Thapanee SAMPHUMPHUANG ◽  
Thanyaporn SOTESARITKUL ◽  
Suriyan CHA-UM
Keyword(s):  
Plant Growth ◽  
Growth Regulation ◽  
Growth Traits ◽  
Curcuma Longa ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Negative Relationship ◽  
Foliar Spray ◽  
Biotic And Abiotic Stress ◽  
Total Soluble Sugar

Paclobutrazol (PBZ) is a member of plant growth retardants, commonly applied for growth regulation, yield improvement, and biotic and abiotic stress alleviation. However, the effects of PBZ on turmeric (Curcuma longa L.; Zingiberaceae), a rhizomatous herb, have not been well established. The objective of this investigation was to gain a better understanding of the effect of PBZ on two different varieties of turmeric plants, ‘Surat Thani’ (‘URT’; high curcuminoids >5% w/w) and ‘Pichit’ (‘PJT’; low curcuminoids <3% w/w). Pseudostem height of cv. ‘PJT’ treated by 340 µM PBZ was significantly decreased by 14.82% over control, whereas it was unchanged in cv. ‘URT’. Interestingly, leaf greenness (SPAD value), maximum quantum yield of PSII (Fv/Fm) and photon yield of PSII (ΦPSII) in cv. ‘PJT’ treated by 340 µM PBZ were significantly elevated by 1.47, 1.28 and 1.23 folds, over control respectively. Net photosynthetic rate (Pn) in cv. ‘PJT’ declined by 38.58% (340 µM PBZ) over control, as a result of low levels of total soluble sugars (TSS; 127.8 mg g-1 DW) in turmeric rhizome. A positive relation between photosynthetic abilities and aerial fresh weight was demonstrated. In addition, a negative relationship between TSS and total curcuminoids was evidently found (R2 = 0.4524). Curcuminoids yield in turmeric rhizomes significantly dropped, depending on the degree of exogenous foliar PBZ applications. In summary, cv. PJT was found to be very sensitive to PBZ application, whereas rhizome yield and growth traits and high amount of curcuminoids were retained in cv. ‘URT’. Plant growth retention in turmeric cv. ‘URT’ using 170 mM PBZ foliar spray without negative effects on rhizome biomass and total curcuminoids content was demonstrated.

scholarly journals The Role of Saccharides in the Mechanisms of Pathogenicity of Fusarium oxysporum f. sp. lupini in Yellow Lupine (Lupinus luteus L.)

2020 ◽  
Vol 21 (19) ◽  
pp. 7258
Author(s):  
Magda Formela-Luboińska ◽  
Dorota Remlein-Starosta ◽  
Agnieszka Waśkiewicz ◽  
Zbigniew Karolewski ◽  
Jan Bocianowski ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Pathogenic Fungus ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Lupinus Luteus ◽  
High Sugar ◽  
Embryo Axes ◽  
Yellow Lupine ◽  
Sugar Levels

The primary aim of this study was to determine the relationship between soluble sugar levels (sucrose, glucose, or fructose) in yellow lupine embryo axes and the pathogenicity of the hemibiotrophic fungus Fusarium oxysporum f. sp. Schlecht lupini. The first step of this study was to determine the effect of exogenous saccharides on the growth and sporulation of F. oxysporum. The second one focused on estimating the levels of ergosterol as a fungal growth indicator in infected embryo axes cultured in vitro on sugar containing-medium or without it. The third aim of this study was to record the levels of the mycotoxin moniliformin as the most characteristic secondary metabolite of F. oxysporum in the infected embryo axes with the high sugar medium and without it. Additionally, morphometric measurements, i.e., the length and fresh weight of embryo axes, were done. The levels of ergosterol were the highest in infected embryo axes with a sugar deficit. At the same time, significant accumulation of the mycotoxin moniliformin was recorded in those tissues. Furthermore, it was found that the presence of sugars in water agar medium inhibited the sporulation of the pathogenic fungus F. oxysporum in relation to the control (sporulation of the pathogen on medium without sugar), the strongest inhibiting effect was observed in the case of glucose. Infection caused by F. oxysporum significantly limited the growth of embryo axes, but this effect was more visible on infected axes cultured under sugar deficiency than on the ones cultured with soluble sugars. The obtained results thus showed that high sugar levels may lead to reduced production of mycotoxins by F. oxysporum, limiting infection development and fusariosis.

Cold hardiness of olive (Olea europaeaL.) cultivars in cold-acclimated and non-acclimated stages: seasonal alteration of soluble sugars and phospholipids

2009 ◽  
Vol 147 (4) ◽  
pp. 459-467 ◽  
Author(s):  
H. GULEN ◽  
A. CANSEV ◽  
A. ERIS
Keyword(s):  
Cold Acclimation ◽  
Cold Hardiness ◽  
Reducing Sugars ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Total Soluble Sugar ◽  
Cold Tolerant ◽  
Olive Cultivars ◽  
Antioxidative Enzyme Activities ◽  
Leaf Tissues

SUMMARYIn many plant species, several physiological and biochemical changes occur during low-temperature-induced cold acclimation. A previous study with olive cultivars (Cansevet al.2009) demonstrated a correlation between the level of accumulation of certain leaf proteins besides antioxidative enzyme activities and cold hardiness of the cultivars. The present paper analysed soluble sugar (SS) and phospholipid (PL) contents of cold-acclimated (CA) and non-acclimated (NA) leaf tissues in order to explain the mechanism of cultivar-dependent response to cold in olive. In general, cold acclimation significantly increased total soluble sugar (TSS), reducing sugars and sucrose contents of all cultivars to various extents depending on the cold hardiness of cultivars. In addition, TSS, reducing sugars and sucrose contents in cold-tolerant cultivars were significantly increased, whereas TSS, reducing sugars and sucrose contents in cold-sensitive cultivars either did not change or increased slightly in CA stage compared with those in NA stage. Even though reducing sugars were the major soluble sugar in olive leaves, levels of sucrose accumulations in CA stage compared with those in NA stage were greater than those observed in reducing sugars accumulation. Changes in levels of total PL, as well as the three individual PL fractions phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI), were investigated in olive leaf tissues. Significant increases in levels of PC and PE fractions during CA compared with those in NA stage suggested that PC and PE maintained the cold hardiness of olive cultivars more effectively than did PI. Although the precise mechanisms by which olive responds to cold may still be open to discussion, soluble sugars and PL are clearly important in the ability of olive cultivars to stand against cold stress.

scholarly journals IMBIBITION, ELECTROLYTE LEAKAGE, AND GERMINATION OF SWEET CORN HYBRIDS CARRYING sh2 MUTANT ENDOSPERM

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1134b-1134
Author(s):  
Carlos A. Parera ◽  
Daniel J. Cantliffe
Keyword(s):  
Seed Quality ◽  
Sweet Corn ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Germination Percentage ◽  
High Concentration ◽  
Corn Hybrids ◽  
Total Soluble Sugar ◽  
Seed Leachate

Seeds of two shrunken-2 (sh2) sweet corn (Zea mays L.) cultivars, Crisp N' Sweet 711 and How Sweet It Is were used to analyze seed quality factor differences between the cultivars. Negative correlations occurred among germination percentage and imbibition, electric conductivity, potassium concentration and total soluble sugars of the seed leachate. Imbibition and total soluble sugar in the leachate significantly increased as imbibition temperature increased from 5°C to 25°C in both cultivars. A significant increase in conductivity of the leachate also occurred in `Crisp N' Sweet 711' when temperature increased. Cracks in the seed coat were more frequent in `How Sweet It Is' than `Crisp N' Sweet 711'. The higher concentrations of soluble sugars in the seed, greater imbibition rate, leakage conductivity, potassium and sugar concentration in the leachate may have been directly related to the poorer seed quality of `How Sweet It Is'. The alteration in cell membrane structure caused by a rapid water uptake in `How Sweet It Is' may have led to the high concentration of electrolytes in the seed leachate. This, in turn, might provide a greater nutritive subtrate for fungi development.

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