Ontogeny of shoot regenerants on excised immature peach embryos

1992 ◽  
Vol 72 (2) ◽  
pp. 497-506 ◽  
Author(s):  
K. E. Schneider ◽  
D. Speranzini ◽  
A. R. Biggs
Keyword(s):  
Prunus Persica ◽  
Shoot Formation ◽  
Starch Granules ◽  
Indirect Organogenesis ◽  
Meristematic Cells ◽  
Friable Callus ◽  
Nodular Callus ◽  
Parental Material ◽  
Nodular Tissue ◽  
Vascular Connections

Excised immature peach embryos when cultured on an auxin-containing medium produced both a friable callus and a nodular callus. Upon further subculturing on an auxin-reduced medium only the nodular callus differentiated into highly regenerative nodular callus. This callus comprised dense meristematic cells which arose near or at the surface of the nodular tissue. Cells containing starch granules often surrounded these meristemoid areas. Shoots could be induced to form on shoot-inducing medium from the meristemoid areas. Vascular connections developed between the parental material and the shoot. The friable callus never appeared to differentiate into a nodular callus on either friable callus or nodular callus medium, nor were plantlets derived from this tissue on shoot-inducing medium. No stages of somatic embryogenesis were ever apparent. The evidence supports the view that the regenerative shoots were derived from indirect organogenesis on nodular callus derived from the embryonic tissue.Key words: Organogenesis, Prunus persica (L.) Batsch, shoot formation

scholarly journals Factors Affecting the Regeneration, via Organogenesis, and the Selection of Transgenic Calli in the Peach Rootstock Hansen 536 (Prunus persica × Prunus amygdalus) to Express an RNAi Construct against PPV Virus

Plants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 178 ◽  
Author(s):  
Sabbadini ◽  
Ricci ◽  
Limera ◽  
Baldoni ◽  
Capriotti ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Prunus Persica ◽  
Fluorescent Protein ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Prunus Amygdalus ◽  
Indirect Organogenesis ◽  
Egfp Gene ◽  
Rnai Construct

Prunus spp. is one of the most recalcitrant fruit tree species in terms of in vitro regeneration and transformation, mostly when mature tissues are used as explants. The present study describes the in vitro regeneration via indirect organogenesis, and Agrobacterium tumefaciens-mediated transformation of the peach rootstock Hansen 536 (Prunus persica × Prunus amygdalus) through the use of meristematic bulks (MBs) as starting explants. Efficient adventitious shoot regeneration was obtained when Hansen 536 MBs were cultured on an optimized medium consisting of modified McCown Woody Plant medium (WPM) enriched with 4.4 M 6-Benzyladenine (BA), 0.1 M 1-Naphthaleneacetic acid (NAA) and 6.0 g L−1 plant agar S1000 (B&V). MB slices were used later as starting explants for Agrobacterium-mediated transformation to introduce an RNAi construct “ihp35S-PPV194” against PPV virus. Transgenic events were identified by both green fluorescent protein (GFP) screening and kanamycin selection at different concentrations (0, 17 or 42 M). GFP-fluorescent proliferating callus lines were selected and confirmed to stably express the ihp35S-PPV194::eGFP gene construct by molecular analysis. Although shoot regeneration from these transgenic calli has not been obtained yet, this represents one of the few examples of successful attempts in peach genetic transformation from somatic tissues, and also serves as a useful in vitro system for future gene functional analysis in peach.

scholarly journals AgNO3 Promotes Callus Production and Regeneration of Immature Buffalograss Inflorescence Culture

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 631c-631
Author(s):  
Shuizhang Fei ◽  
Paul E. Read ◽  
Terrance P. Riordan
Keyword(s):  
Callus Formation ◽  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Positive Effects ◽  
Friable Embryogenic Callus ◽  
Friable Callus ◽  
Promotive Effect ◽  
Callus Production ◽  
Regeneration Efficiency

The use of buffalograss [Buchloe dactyloides (Nutt.) Engelm] in home lawns and golf courses has been increasing because of its drought resistance and low growth habit. In vitro regeneration of buffalograss at a high frequency may provide an effective tool to introduce new variation for breeding use. The positive effects of AgNO3 on friable embryogenic callus production and regeneration efficiency is well documented in maize. In order to determine if AgNO3 has the same effect on buffalograss, two vegetatively propagated cultivars, a female `609' and a male `45-3' were tested at three different concentrations of AgNO3 at 5, 10, and 15 mg·L–1 using immature inflorescences as explants. Murashige and Skoog medium supplemented with 2 mg 2,4-D/L was employed as the control medium. Medium containing AgNO3 significantly promoted the production of friable callus for `45-3' with the highest percentage achieved at 10 mg AgNO3/L. AgNO3 medium led to production of significantly larger calli than found for the control. However, no difference was detected among 5, 10, and 15 mg AgNO3/L with regard to the callus formation ability and the size of callus initiated on these three treatments. Calli were then transferred to MS medium supplemented with BA at 0.1, 0.5 or 1.0 mg·L–1 to induce shoot formation. BA at 0.5 mg·L–1 gave the best differentiation response. Calli formed in the absence of AgNO3 produced more shoots per callus, but more calli were produced in the presence of AgNO3, and the overall regeneration efficiency was much higher with AgNO3 at 10 mg·L–1. In contrast, AgNO3 showed no promotive effect on callus production and regeneration for `609'.

Improved procedures for in vitro regeneration and for phenotypic analysis in the model legume Lotus japonicus

2005 ◽  
Vol 32 (6) ◽  
pp. 529 ◽  
Author(s):  
Ani Barbulova ◽  
Enrica D'Apuzzo ◽  
Alessandra Rogato ◽  
Maurizio Chiurazzi
Keyword(s):  
In Vitro Regeneration ◽  
Lotus Japonicus ◽  
Shoot Formation ◽  
Direct Somatic Embryogenesis ◽  
Phenotypic Analysis ◽  
Indirect Organogenesis ◽  
Model Legume ◽  
Mesorhizobium Loti ◽  
Efficient Gene

As a prerequisite for the development of an efficient gene transfer methodology, the possibility of inducing direct somatic embryogenesis in Lotus japonicus (Regel) K. Larsen explants was investigated. Petiole bases, cotyledons, hypocotyls and stem segments were cultivated in the presence of different amounts of benzylaminopurine (BAP) and / or thidiazuron (TDZ). Regeneration was achieved differentially in the different explants and a higher efficiency of shoot formation was obtained with TDZ. By maintaining the same TDZ regime a second cycle of morphogenesis was achieved and the histological analysis of these structures indicated unambiguously their somatic embryogenic nature. Thidiazuron was also tested as an agent to improve the kinetics of shoot formation in a Lotus japonicus transformation–regeneration procedure based on indirect organogenesis. A very significant, highly reproducible, increase in the rate of the shoot formation was observed in independent transformation experiments. We also present an extensive analysis of the feasibility and reproducibility of an in vitro procedure, which can be very useful for the screening of symbiotic phenotypes in transgenic Lotus plants and for the analysis of the cascade of molecular and cytological events occurring soon after Mesorhizobium loti infection.

scholarly journals In vitro Propagation of Abrus precatorius L. - A Rare Medicinal Plant of Chittagong Hill Tracts

1970 ◽  
Vol 17 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Animesh Biswas ◽  
Mohashweta Roy ◽  
MA Bari Miah ◽  
SK Bhadra
Keyword(s):  
Medicinal Plant ◽  
Adventitious Shoots ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Indirect Organogenesis ◽  
Abrus Precatorius ◽  
Nodular Callus ◽  
Half Strength ◽  
Cut Surface

An efficient protocol was developed for in vitro propagation of Abrus precatorius L. through induction of indirect organogenesis in nodal segment derived callus tissue. Yellowish-green nodular callus was induced at the cut surface of the nodal segments cultured on MS fortified with 5.0 mg/l BAP and 0.5 mg/l NAA. The callus differentiated into adventitious shoots when it was subcultured on to MS supplemented with 3.0 mg/l BAP + 0.5 mg/l Kn + 0.5 mg/l NAA. On an average 6.87 ± 0.26 shoots/culture developed. These microshoots were rooted in half-strength MS containing 1.0 mg/l IBA and the rooted plantlets were transferred to soil after proper acclimatization.Key words: Abrus precatorius, Medicinal plant, Callus culture, PropagationDOI = 10.3329/ptcb.v17i1.1121Plant Tissue Cult. & Biotech. 17(1): 59-64, 2007 (June)

scholarly journals Growth and In Vitro Propagation of Peach Plants Transformed with the Shooty Mutant Strain of Agrobacterium tumefaciens

HortScience ◽  
1998 ◽  
Vol 33 (5) ◽  
pp. 897-899 ◽  
Author(s):  
F.A. Hammerschlag ◽  
A.C. Smigocki
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Mutant Strain ◽  
In Vitro Propagation ◽  
Prunus Persica ◽  
Shoot Formation ◽  
Fresh Mass ◽  
Axillary Shoot ◽  
Shoot Cultures ◽  
Cytokinin Biosynthesis

Peach [Prunus persica (L.) Batsch] plants #94-1, #99-1, and #40-1, carrying a cytokinin biosynthesis (ipt) gene following transformation with the shooty mutant strain of Agrobacterium tumefaciens, were evaluated for altered growth habit and axillary shoot formation, both in vitro and in the greenhouse. After 9 weeks of in vitro propagation on four different levels of 6-benzyladenine (BA), only transformant #99-1 exhibited significantly greater axillary shoot formation (on 10 μm BA), and significantly greater fresh mass (on 3,10, and 30 μm BA) than the control #RG-3. Tolerance to a supra-optimal (30 μm) concentration of BA was indicated by fresh mass increases for #99-1 shoot cultures. Delayed senescence on 0 μm BA was exhibited by 87% of the transformants, but by only 12% of the control plants. Greenhouse-grown #99-1 and #40-1 were significantly shorter than #RG-3 plants at 6 weeks and at 1 year, but only #40-1 exhibited significantly greater branching than the controls. Chemical names used: 6-benzyladenine (BA); isopentenyl transferase (ipt).

scholarly journals Indirect organogenesis and somatic embryogenesis for regeneration of Rauvolfia serpentina L. from root explants

2020 ◽  
Vol 49 (4) ◽  
pp. 1021-1027
Author(s):  
Tanjina Akhtar Banu ◽  
Salim Khan ◽  
Barna Goswami ◽  
Sadia Afrin ◽  
Ahashan Habib ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Aerial Part ◽  
Shoot Formation ◽  
Ms Medium ◽  
Indirect Organogenesis ◽  
Rauvolfia Serpentina ◽  
Root Explants

Highest frequencies (75%) of friable embryogenic nodular calli were recorded from the root explants in the presence of MS + 4.0 mg/l BAP and 4.0 mg/l NAA. In combinations of BAP and NAA 45.4% callus produced direct plantlets formation and 36.3% only from aerial part of shoots. MS medium with 1.0 mg/l BAP and 0.5 mg/l Kn produced compact callus where 13.3% produced direct plantlets formation and 45.7% produced only shoot formation. Well-developed globular somatic embryos were found to form when the callus were cultured more than 6 - 8 weeks on MS with 1.0 mg/l BAP and 0.5 mg/l Kn. After acclimatization the well rooted plantlets were transferred to pot and grown successfully in soil.

Ethylene enhances shoot formation in cultures of the peach rootstock GF-677 (Prunus persica x P. amygdalus)

1995 ◽  
Vol 15 (1-2) ◽  
pp. 87-90 ◽  
Author(s):  
Kortessa Dimasi-Theriou ◽  
Athanasios S. Economou
Keyword(s):  
Prunus Persica ◽  
Shoot Formation

scholarly journals TISSUE-CULTURE AND PROTOPLAST ISOLATION IN ASPARAGUS DENSIFLORUS L.

HortScience ◽  
1993 ◽  
Vol 28 (4) ◽  
pp. 259G-259
Author(s):  
Mustapha Benmousea ◽  
Yves Desjardine
Keyword(s):  
Tissue Culture ◽  
Cell Wall ◽  
Plant Growth ◽  
Genetic Material ◽  
Protoplast Isolation ◽  
M Cells ◽  
Ms Medium ◽  
Callus Cell ◽  
Indirect Organogenesis ◽  
Friable Callus

We have developed tissue culture and protoplasts isolation protocols for Asparagus densiflorus in order to use this genetic material in the breeding of Asparagus officinalis. For tissue-culture of A. densiflorus, the conditions which optimize the induction and the production of callus are a full MS medium with 1 mg/L of both pCPA and BAP and 0.5 mg/L of thiamine. HCL in the dark. on this medium, we obtained a friable white callus. Indirect organogenesis was obtained if pCPA was omitted from the medium. Replacement of the plant growth regulators by 2,4-D and Kinetin produced a hard and compact callus which did not differentiate. Protoplast have been isolated from 10 days old friable callus. cell wall was digested with 0.3% macerase, 1% cellulase and 0.8% rhozyme for a period of 16h at a temperature of 27°C in a CPW medium. Protoplast yield was 2 ×106 protoplasts/g callus. osmolarity of the digestion solution was 0.8 M provided with a mixture of glucose (0.6 M) and mannitol (0.2 M). cells were then plated at a density of 1 × 105 cells per ml. Microcolonies formed on a 1/2 MS medium with 0,5 mg/L NAA and ZEA and 1 g/L glutamine in the dark.

scholarly journals Induction Shoots From Callus of Cucumber Apple (Cucumis sp.) Using a Combination of Benzil Amino Purine and Naphtalene Acetic Acid Concentrations In Vitro

Mangifera Edu ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 103-120
Author(s):  
Fransisca Natalia Erzalinda Mendrofa ◽  
Nurcahyo Widyodaru Saputro ◽  
Lutfi Afifah
Keyword(s):  
Acetic Acid ◽  
Experimental Method ◽  
Research Method ◽  
Shoot Formation ◽  
Naphthalene Acetic Acid ◽  
Indirect Organogenesis ◽  
Shoot Height ◽  
Best Response ◽  
Benzyl Amino Purine

Research on indirect organogenesis of cucumber apple (Cucumis sp.) was conducted from March to May 2020. This study aims to find the best combination between Benzyl Amino Purine (BAP) and Naphthalene Acetic Acid (NAA) on the growth of cucumber apple (Cucumis) sp.) on Gamborg media (B5) by organogenesis. The callus used came fromcucumber seeds explants with a concentration of NAA 2,0 mg/l + BAP 0,2 mg/l using Murashige and Skoog media. The research method used is an experimental method with nonparametric statistics with 24 treatments repeated three times and analyzed descriptively using the Kruskal Wallis test. The results showed that the best treatment of cucumber apple shoots was the combination of NAA and BAP, namely the B4N2 treatment (2,5 ppm BAP + 0,20 ppm NAA) with shoot height (0,45 cm). At a single concentration, a concentration of 1 ppm BAP was able to provide a faster effect on shoot formation time, namely at 24 days after initiation (hsi) and the best response to shooting height (0,5cm).

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