RESPONSE OF NEAR-ISOGENIC PEA CULTIVARS TO INFECTION BY Fusarium oxysporum f. sp. pisi RACES 1 AND 5

1989 ◽  
Vol 69 (4) ◽  
pp. 1335-1346 ◽  
Author(s):  
M. CHARCHAR ◽  
J. M. KRAFT
Keyword(s):  
Fusarium Oxysporum ◽  
Lateral Roots ◽  
Dominant Gene ◽  
Fungal Growth ◽  
Resistance Response ◽  
Resistant Cultivars ◽  
Tap Root ◽  
Race 1 ◽  
Xylem Elements ◽  
Root Apices

Near-isogenic pea cultivars, differing by a single dominant gene for resistance (R) or susceptibility (S) to Fusarium oxysporum f. sp. pisi race 1 (’M410’-S, ’Vantage’-R) or race 5 (’Sundance’-S, ’Sundance II’-R), were evaluated for their response to infection. The response of resistant cultivars to each race was similar. Colonies of both races were isolated in higher numbers from tap and lateral root apices of susceptible as compared with resistant cultivars. Internal tap root and hypocotyl invasion occurred in all cultivars tested. However, lateral roots and stems of resistant cultivars were not infected as compared with susceptible lines. Surface colonization of tap and lateral roots of the resistant cultivars was significantly less than with the susceptible cultivars. Scanning electron microscopy revealed that gel-like material completely sealed off xylem elements in lateral roots, epicotyls, and aboveground stems of resistant cultivars. In contrast, intense mycelial invasion, without the formation of gels, occurred in susceptible cultivars. Conidial germination and germ-tube growth of both race 1 and race 5 were stimulated by xylem fluids from the susceptible, but not from the resistant cultivars. A resistance response based on physical containment and reduced fungal growth was evident.Key words: Fusarium wilt, host response, Pisum sativum

scholarly journals Biological and Molecular Characterization of Fusarium oxysporum f. sp. lycopersici Divides Race 1 Isolates into Separate Virulence Groups

1999 ◽  
Vol 89 (2) ◽  
pp. 156-160 ◽  
Author(s):  
Jurriaan J. Mes ◽  
Emma A. Weststeijn ◽  
Frits Herlaar ◽  
Joep J. M. Lambalk ◽  
Jelle Wijbrandi ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Random Amplified Polymorphic Dna ◽  
Vegetative Compatibility ◽  
Avirulence Gene ◽  
Resistance Response ◽  
Vegetative Compatibility Groups ◽  
Small Set ◽  
Race 1 ◽  
Race 2 ◽  
Susceptible Tomato

A collection of race 1 and race 2 isolates of Fusarium oxysporum f. sp. lycopersici was screened for vegetative compatibility and characterized by random amplified polymorphic DNA (RAPD) analysis to establish the identity and genetic diversity of the isolates. Comparison of RAPD profiles revealed two main groups that coincide with vegetative compatibility groups (VCGs). In addition, several single-member VCGs were identified that could not be grouped in one of the two main RAPD clusters. This suggests that F. oxysporum f. sp. lycopersici is a polyphyletic taxon. To assign avirulence genotypes to race 1 isolates, they were tested for their virulence on a small set of tomato lines (Lycopersicon esculentum), including line OT364. This line was selected because it shows resistance to race 2 isolates but, unlike most other race 2-resistant lines, susceptibility to race 1 isolates. To exclude the influence of other components than those related to the race-specific resistance response, we tested the virulence of race 1 isolates on a susceptible tomato that has become race 2 resistant by introduction of an I-2 transgene. The results show that both line OT364 and the transgenic line were significantly affected by four race 1 isolates, but not by seven other race 1 isolates nor by any race 2 isolates. This allowed a subdivision of race 1 isolates based on the presence or absence of an avirulence gene corresponding to the I-2 resistance gene. The data presented here support a gene-for-gene relationship for the interaction between F. oxysporum f. sp. lycopersici and its host tomato.

scholarly journals Fusarium Yellows and Turnip Mosaic Virus Resistance in Brassica rapa and B. juncea

HortScience ◽  
1997 ◽  
Vol 32 (5) ◽  
pp. 927-930 ◽  
Author(s):  
Robert G. Fjellstrom ◽  
Paul H. Williams
Keyword(s):  
Fusarium Oxysporum ◽  
Mosaic Virus ◽  
Brassica Rapa ◽  
Virus Resistance ◽  
Turnip Mosaic Virus ◽  
Resistant Cultivars ◽  
Disease Resistant ◽  
Race 1

Thirty-seven Brassica rapa L. and B. juncea L. lines from nine subspecies were tested for their reaction to two pathotypes of Fusarium yellows (Fusarium oxysporum Schlecht. f. sp. conglutinans (Wr.) Snyd. & Hans. race 1 and F.o. f. sp. raphani Kend. & Snyd. A subset of 16 lines from these same vegetable types were tested for their reaction to four strains of turnip mosaic virus (TuMV-C1, C2, C3, and C4). Resistance to both Fusarium pathotypes was widespread in these Brassica subspecies, whereas resistance to any strain of TuMV was uncommon. The broad availability of resistance to Fusarium yellows and scarcity of resistance to TuMV necessitate different approaches to obtain disease-resistant cultivars.

A single dominant gene/locus model for control of Fusarium oxysporum f. sp. lactucae race 1 resistance in lettuce (Lactuca sativa)

Euphytica ◽  
2019 ◽  
Vol 215 (7) ◽  
Author(s):  
Cléia S. Cabral ◽  
Maria Esther de N. Fonseca ◽  
Valter R. Oliveira ◽  
Leonardo S. Boiteux ◽  
Ailton Reis
Keyword(s):  
Fusarium Oxysporum ◽  
Lactuca Sativa ◽  
Gene Locus ◽  
Dominant Gene ◽  
Single Dominant Gene ◽  
Locus Model ◽  
Race 1

scholarly journals The Endophytic Strain Fusarium oxysporum Fo47: A Good Candidate for Priming the Defense Responses in Tomato Roots

2013 ◽  
Vol 26 (8) ◽  
pp. 918-926 ◽  
Author(s):  
Sébastien Aimé ◽  
Claude Alabouvette ◽  
Christian Steinberg ◽  
Chantal Olivain
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Quantitative Polymerase Chain Reaction ◽  
Fungal Growth ◽  
Defense Responses ◽  
Plant Defense Responses ◽  
Tomato Roots ◽  
Tap Root

The protective Fusarium oxysporum strain Fo47 is effective in controlling Fusarium wilt in tomato. Previous studies have demonstrated the role of direct antagonism and involvement of induced resistance. The aim of the present study was to investigate whether priming of plant defense responses is a mechanism by which Fo47 controls Fusarium wilt. An in vitro design enabled inoculation of the tap root with Fo47 and the pathogenic strain (Fol8) at different locations and different times. The expression levels of six genes known to be involved in tomato defense responses were quantified using reverse-transcription quantitative polymerase chain reaction (qPCR). Three genes—CHI3, GLUA, and PR-1a—were overexpressed in the root preinoculated with Fo47, and then challenged with Fol8. The genes GLUA and PR-1a were upregulated in cotyledons after inoculation of Fo47. Fungal growth in the root was assessed by qPCR, using specific markers for Fo47 and Fol8. Results showed a reduction of the pathogen growth in the root of the tomato plant preinoculated with Fo47. This study demonstrated that priming of tomato defense responses is one of the mechanisms of action of Fo47, which induces a reduced colonization of the root by the pathogen.

scholarly journals Vegetative Compatibility Groups of Fusarium oxysporum f. sp. lactucae from Lettuce

Plant Disease ◽  
2005 ◽  
Vol 89 (3) ◽  
pp. 237-240 ◽  
Author(s):  
Matias Pasquali ◽  
Flavia Dematheis ◽  
Giovanna Gilardi ◽  
Maria Lodovica Gullino ◽  
Angelo Garibaldi
Keyword(s):  
Fusarium Oxysporum ◽  
Genetic Relatedness ◽  
The United States ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Group ◽  
Vegetative Compatibility Groups ◽  
Compatibility Group ◽  
Race 1

Fusarium oxysporum f. sp. lactucae, the causal agent of Fusarium wilt of lettuce, has been reported in three continents in the last 10 years. Forty-seven isolates obtained from infected plants and seed in Italy, the United States, Japan, and Taiwan were evaluated for pathogenicity and vegetative compatibility. Chlorate-resistant, nitrate-nonutilizing mutants were used to determine genetic relatedness among isolates from different locations. Using the vegetative compatibility group (VCG) approach, all Italian and American isolates, type 2 Taiwanese isolates, and a Japanese race 1 were assigned to the major VCG 0300. Taiwanese isolates type 1 were assigned to VCG 0301. The hypothesis that propagules of Fusarium oxysporum f. sp. lactucae that caused epidemics on lettuce in 2001-02 in Italian fields might have spread via import and use of contaminated seeds is discussed.

Allelic analysis of stripe rust resistance genes on wheat chromosome 2BS

Genome ◽  
2008 ◽  
Vol 51 (11) ◽  
pp. 922-927 ◽  
Author(s):  
P. G. Luo ◽  
X. Y. Hu ◽  
Z. L. Ren ◽  
H. Y. Zhang ◽  
K. Shu ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Dominant Gene ◽  
Wheat Chromosome ◽  
Stripe Rust Resistance ◽  
Resistance Response ◽  
Rust Resistance Genes ◽  
Yr Genes

Stripe rust, caused by Puccinia striiormis Westend f. sp. tritici, is one of the most important foliar diseases of wheat ( Triticum aestivum L.) worldwide. Stripe rust resistance genes Yr27, Yr31, YrSp, YrV23, and YrCN19 on chromosome 2BS confer resistance to some or all Chinese P. striiormis f. sp. tritici races CYR31, CYR32, SY11-4, and SY11-14 in the greenhouse. To screen microsatellite (SSR) markers linked with YrCN19, F1, F2, and F3 populations derived from cross Ch377/CN19 were screened with race CYR32 and 35 SSR primer pairs. Linkage analysis indicated that the single dominant gene YrCN19 in cultivar CN19 was linked with SSR markers Xgwm410, Xgwm374, Xwmc477, and Xgwm382 on chromosome 2BS with genetic distances of 0.3, 7.9, 12.3, and 21.2 cM, respectively. Crosses of CN19 with wheat lines carrying other genes on chromosome 2B showed that all were located at different loci. YrCN19 is thus different from the other reported Yr genes in chromosomal location and resistance response and was therefore named Yr41. Prospects and strategies of using Yr41 and other Yr genes in wheat improvement for stripe rust resistance are discussed.

scholarly journals Outbreak of Fusarium oxysporum f. sp. lycopersici race 3 in commercial fresh-market tomato fields in Rio de Janeiro State, Brazil

2007 ◽  
Vol 25 (3) ◽  
pp. 451-454 ◽  
Author(s):  
Ailton Reis ◽  
Leonardo S Boiteux
Keyword(s):  
Fusarium Oxysporum ◽  
Rio De Janeiro ◽  
Present Report ◽  
Fresh Market ◽  
Solanum Pennellii ◽  
Espírito Santo ◽  
Race 1 ◽  
Serious Disease ◽  
Espirito Santo ◽  
Janeiro State

Fusarium wilt, caused by three races of Fusarium oxysporum f. sp. lycopersici, is one of the most important tomato diseases. In Brazil, all three races were reported, however, race 3 has been so far restricted only to Espírito Santo State. In the present work, seven F. oxysporum isolates obtained from wilted plants of the race 1 and 2-resistant tomato hybrids 'Giovana', 'Carmen' and 'Alambra' in São José de Ubá and Itaocara (Rio de Janeiro State, Brazil) were characterized at race level. Virulence assays were performed using a set of race differential cultivars: 'Ponderosa' (susceptible to all races), 'IPA-5' (resistant to race 1), 'Floradade' (resistant to races 1 and 2), 'BHRS-2,3' (resistant to all three races). Two wild tomato accessions (Solanum pennellii 'LA 716' e S. chilense 'LA 1967') previously reported as resistant to all Brazilian isolates of F. oxysporum f. sp. lycopersici were also evaluated. Isolates from São José de Ubá and Itaocara were highly virulent to 'Ponderosa', 'IPA-5' and 'Floradade'. They were also able to infect a few plants of 'BHRS-2,3', inducing vascular browning and wilt symptoms. Solanum pennellii and S. chilense accessions displayed an extreme (immune-like) resistant response. These results indicated that all seven isolates could be classified as F. oxysporum f. sp. lycopersici race 3, expanding the geographical distribution of this pathogen within Brazil. The hypothesis of transmission via contaminated seeds is reinforced after the present report, which confirms the almost simultaneous outbreak of race 3 in two geographically isolated tomato-growing areas in Brazil (Espirito Santo and Rio de Janeiro). Evaluation of commercial seed lots imported into Brazil for contamination with the pathogen would be necessary in order to avoid nation-wide spread of this serious disease.

Sign in / Sign up

Export Citation Format

Share Document