R-nj ALEURONE COLOR SELECTION AND NITROGEN RESPONSE IN OPAQUE-2 MAIZE
Field experiments were conducted to determine if mass selection for degree of aleurone anthocyanin pigmentation controlled by R-nj could improve agronomic deficiencies associated with the o2 (opaque) phenotype of a maize (Zea mays L.) synthetic (NDSE). Divergent mass selection was used to develop high color (HC), low color (LC), and randomly sampled (RC) check substrains which were tested in 1982 at two low-fertility, pollen isolated sites (a clay loam, Vertic Haplaquoll with 7.2 pH and a silt loam, Aerie Calciaquoll with a 7.6 pH at Fargo and Casselton, N.D., respectively). Grain yield and agronomic traits were estimated for each of four N application rates at each site. N fertilizer increased grain yields and kernel weights. High color strains yielded highest at 136 kg ha−1 of N, outproducing RC strains. At 204 kg ha−1 of N, yield of HC strains declined and LC strains out-yielded HC strains. Low color selection significantly increased test weight, kernel weight, cob diameter, seedling emergence, and shelling ratio compared to RC strains in NDSE while HC selection increased kernel weight, cob diameter, and reduced ear moisture and protein contents compared to RC selection. Selection for LC improved several traits which were deficient in opaque-2 maize, and use of the R-nj gene may be useful in improving opaque-2 maize.Key words: Aleurone, anthocyanin, correlated response, ear moisture, mass selection, maize.