COMPARISON OF PHOTOSYNTHESIS IN NORMAL AND TRIAZINE-RESISTANT Brassica

1987 ◽  
Vol 67 (2) ◽  
pp. 457-466 ◽  
Author(s):  
S. L. A. HOBBS

In spaced field plantings, triazine-resistant types of Brassica campestris L. and B. napus L. had a carbon dioxide exchange rate (CER) 28% lower in 1983 and 25% lower in 1984 than normal (triazine-susceptible) types. In plots simulating agronomic spacings in 1984, the difference between CER in normal and resistant types was 17% for B. campestris, 14% for B. napus and 13% for B. juncea L. Differences were apparent throughout the season and were not associated with any particular stage of growth. Resistant progeny from reciprocal crosses between resistant and susceptible plants of B. napus exhibited reduced CER at all levels of photosynthetically active radiation and at all temperatures. There was no significant difference between plant types for chlorophyll a + b content or chlorophyll a/b ratio. Shoot dry weight, stomatal resistance and specific leaf weight were higher in the normal types, but there was no difference between types in either relative growth rate or net assimilation rate. The reduced biomass was not therefore linked to reduced CER.Key words: Carbon dioxide exchange rate, herbicide, oilseed, rapeseed

1986 ◽  
Vol 66 (3) ◽  
pp. 465-472 ◽  
Author(s):  
S. L. A. HOBBS

Compensation between carbon dioxide exchange rate per unit photosynthetic area (CER) and total photosynthetic area (TPA) of a plant was examined in field-grown pea (Pisum sativum L.). Eight near-isogenic lines of cv. Alaska, representing all possible phenotypes of the genes af (leaflets transformed to tendrils), st (reduced stipule area) and tl (tendrils transformed to leaflets), were examined. The CER was measured on the leaflets (AfAf), tendrils (afafTlTl) or minute leaflets (afaftltl). The TPA was significantly reduced by the st gene in AfAf types (normal leaflets) with an apparently associated increase in CER. The st gene also significantly reduced the TPA in afaf types but there was no associated increase in CER. Tendrils had a lower CER than normal leaflets and comprised 22% of the TPA of the semi-leafless (afafStStTlTl) type. Crosses were made between a semi-leafless pea and four normal-leafed types previously selected for high or low CER. The CER means (normal leaflets) of the F1 progeny showed variability which was related to parental values. This was also true for the CER means (tendrils) of the populations of semi-leafless F2 segregants showing that genetic variability for CER can exist in tendrils. In the F2, tendril CER was correlated negatively to stomatal resistance and positively to chlorophyll content and final shoot dry weight (biomass). Genetic improvement in CER may be important when a plant ideotype requires substantial reduction in TPA.Key words: Photosynthesis, pea, chlorophyll content, stomatal resistance, Pisum sativum


1972 ◽  
Vol 23 (2) ◽  
pp. 225 ◽  
Author(s):  
KC Hodgkinson ◽  
NG Smith ◽  
GE Miles

The net carbon dioxide exchange rate (NCE) of lucerne (Medicago sativa cv. Hunter River) stubble leaves was found to be low (c. 55 ng CO2 cm-2 sec-1) immediately after removing shoots 15 cm above the stem base. However, within several days the NCE rose to a maximum of 130 ng CO2 cm-2 sec-1 on day 8. This peak rate was similar to that of recently expanded new leaves and held for about 3 days before declining. Leaves of similar age and position on uncut plants showed a steady decline in NCE. Leaf resistance, r'l, was low (c. 0.8 sec cm-1) and did not change as the NCE increased during the first 8 days. Mesophyll resistance, r'm, to carbon dioxide diffusion was initially high and declined from c. 9 to 2 sec cm-1 during this period. Comparison of dry weight changes of plant parts after stubble leaves were either retained or removed suggested that the tap-root benefited most by stubble leaf retention. The contribution to new shoots from stubble leaves appeared to be slight. However, when stubble leaves were exposed to 14CO2 on day 11, photosynthate was mainly exported into the shoot arising from the axil of the exposed leaf. This suggests that stubble leaves substitute, in part or completely, for the supply of carbohydrate to stubble shoots normally derived from reserves mobilized in the tap-root. The likely causes of rejuvenation in NCE of stubble leaves after partial shoot removal are discussed along with the significance of this photosynthetic contribution to regrowth of the lucerne plant.


2007 ◽  
Vol 87 (3) ◽  
pp. 581-585 ◽  
Author(s):  
Ahmet Korkmaz ◽  
Murat Uzunlu ◽  
Ali Riza Demirkiran

Salicylic acid (SA) is a common plant-produced signal molecule that is responsible for inducing tolerance to a number of biotic and abiotic stresses. An experiment was, therefore, conducted to test whether acetyl salicylic acid (ASA) application at various concentrations through seed immersion or foliar spray would protect muskmelon [Cucumis melo L. (Reticulatus Group)] seedlings subjected to chilling stress. Twenty-one-day-old plants pre-treated with ASA (0, 0.1, 0.25, 0.50 or 1.0 mM) were subjected to chilling stress for 72 h at 3 ± 0.5°C. ASA, applied either through seed immersion or foliar spray, was effective within the range of 0.1 to 1 mM in inducing tolerance to chilling stress in muskmelon seedlings; however, there was no significant difference between application methods. ASA significantly and curvilinearly affected all seedling growth and stress indicator variables tested except shoot dry weight. The best protection was obtained from seedlings pre-treated with 0.5 mM ASA. The highest ASA concentration used was slightly less effective in providing chilling stress protection. Even though both methods provided similar means of protection, due to its simplicity and practicality, immersion of muskmelon seeds prior to sowing in 0.5 mM ASA would be a more desirable method to induce tolerance to chilling stress. Key words: Cucumis melo, aspirin, chilling stress tolerance, gas exchange, electrolyte leakage


1975 ◽  
Vol 26 (3) ◽  
pp. 497 ◽  
Author(s):  
EAN Greenwood ◽  
P Farrington ◽  
JD Beresford

The time course of development of a lupin crop was studied at Bakers Hill, Western Australia. The aim was to gain insight into the crop factors influencing yield. Weekly measurements were made of numbers and weights of plant parts, and profiles of roots, leaf area and light interception. A profile of carbon dioxide in the crop atmosphere was taken at the time of maximum leaf area, and the net carbon dioxide exchange (NCE) of pods was estimated for three successive weeks. The crop took 10 weeks to attain a leaf area index (LAI) of 1 and a further 9 weeks to reach a maximum LAI of 3.75, at which time only 33% of daylight reached the pods on the main axis. Once the maximum LAI was attained at week 19, leaf fall accelerated and rapid grain filling commenced almost simultaneously on all of the three orders of axes which had formed pods. Measurements of NCE between pods on the main axis and the air suggest that the assimilation of external carbon dioxide by the pods contributed little to grain filling. Grain dry weight was 2100 kg ha-1 of which 30%, 60% and 10% came from the main axis, first and second order apical axes respectively. Only 23% of the flowers set pods and this constitutes an important physiological limitation to grain yield.


2014 ◽  
Vol 23 (3) ◽  
pp. 236-245 ◽  
Author(s):  
Mauritz Vilhelm Vestberg ◽  
Sanna Kukkonen ◽  
Päivi Parikka ◽  
Dan Yu ◽  
Martin Romantschuk

There is increasing global interest in using compost to suppress soil-borne fungal and bacterial diseases and nematodes. We studied the reproducibility of compost suppressive capacity (SC) against Pythium wilt of cucumber using nine composts produced by the same composting plant in 2008 and 2009. A bioassay was set up in a greenhouse using cucumber inoculated with two strains of Pythium. The composts were used as 20% mixtures (v:v) of a basic steam-sterilized light Sphagnum peat and sand (3:1, v:v). Shoot height was measured weekly during the 5-week experiment. At harvest, the SC was calculated as the % difference in shoot dry weight (DW) between non-inoculated and inoculated cucumbers. The SC was not affected by year of production (2008 or 2009), indicating reproducibility of SC when the raw materials and the composting method are not changed. Differences in shoot height were not as pronounced as those for shoot DW. The results were encouraging, but further studies are still needed for producing compost with guaranteed suppressiveness properties.


Weed Science ◽  
1986 ◽  
Vol 34 (3) ◽  
pp. 338-343 ◽  
Author(s):  
John Cardina ◽  
Nathan L. Hartwig ◽  
Felix L. Lukezic

Two strains of crownvetch (Coronilla variaL. # CZRVA) rhizobia were cultured in vitro with various rates of atrazine [6-chloro-N-ethyl-N′-(1-methylethyl)-1,3,5-triazine-2,4-diamine] and bifenox [methyl 5-(2,4-dichlorophenoxy)-2-nitrobenzoate]. Growth, measured turbidimetrically over 48 h, was similar for both strains. Atrazine and bifenox significantly reduced bacterial growth after 14 and 36 h, respectively, only at the highest concentrations tested (463 μM atrazine and 292 μM bifenox). Since growth of crownvetch rhizobia was apparently not affected by rates of atrazine or bifenox above reasonable soil solution concentrations, it is likely that herbicidal effects on nodulation were due to toxicity to the host plant rather than toxicity to these bacteria. In a growth chamber experiment, total nodule activity (TNA) and carbon dioxide exchange rate (CER) were measured simultaneously in an effort to distinguish direct atrazine effects on nodule function from indirect effects due to inhibition of photosynthesis and a resulting decrease in photosynthate supply to nodules. When 5 and 50 mg atrazine per kg soil were applied to intact plants, CER was severely reduced within 24 h, but similar reductions in TNA were not observed until 48 h after treatment. Total nodule activity was reduced similarly by atrazine and defoliation; the application of atrazine to defoliated plants did not inhibit TNA more than did defoliation alone. The data indicate that reductions in crownvetch nodule activity by atrazine are due to inhibition of photosynthesis or other processes rather than direct toxicity to N fixation.


1973 ◽  
Vol 51 (10) ◽  
pp. 1907-1916 ◽  
Author(s):  
S. B. Ku ◽  
L. A. Hunt

Effects of temperature on (1) physical characteristics of newly matured leaves throughout regrowth, and (2) net carbon dioxide exchange–irradiance response curves throughout regrowth and throughout the day are described for two alfalfa (Medicago saliva L.) genotypes (AT171 and CC120) grown at 20/15C and 30/25C day/night temperatures and 53 nE cm−2 s−1 irradiance (400–700 nm).Area per leaf increased linearly with increasing leaf number up to the fourth or fifth leaf, and thereafter remained constant. Both specific leaf weight and leaf density were constant for the first four leaves, and increased sharply thereafter, particularly at day/night temperatures of 20/15C. Percentage of leaf water content did not change throughout regrowth at 30/25C, but decreased after leaf 4 at 20/15C. Intercellular space volume fluctuated with leaf number. Leaf area was larger, specific leaf weight, and leaf density were greater, intercellular space volume was higher, and percentage of leaf water content was lower, with plants grown at 20/15C than at 30/25C.The net carbon dioxide exchange rate at 116 nE cm−2 s−1 increased with leaves produced progressively until a peak was reached at leaf 4 or 5 and then decreased. At any given leaf position, net carbon dioxide exchange rate at 116 nE cm−2 s−1 was greater at 20/15C than at 30/25C for AT171, but was the same at both temperatures for CC120. In contrast, net carbon dioxide exchange rate at 76 nE cm−2 s−1 was greater at 20/15C than 30/25C for both genotypes. Net carbon dioxide exchange rates measured in the morning were always lower than those measured in the afternoon regardless of irradiance, genotype, and growth temperature.


1996 ◽  
Vol 121 (4) ◽  
pp. 699-704 ◽  
Author(s):  
Yaping Si ◽  
Royal D. Heins

Sweet pepper (Capsicum annuum `Resistant Giant no. 4') seedlings were grown for 6 weeks in 128-cell plug trays under 16 day/night temperature (DT/NT) regimes from 14 to 26 °C. Seedling stem length, internode length, stem diameter, leaf area, internode and leaf count, plant volume, shoot dry weight (DW), seedling index, and leaf unfolding rate (LUR) were primarily functions of average daily temperature (ADT); i.e., DT and NT had similar effects on each growth or development parameter. Compared to ADT, the difference (DIF, where DIF = DT - NT) between DT and NT had a smaller but still statistically significant effect on stem and internode length, leaf area, plant volume, stem diameter, and seedling index. DIF had no effect on internode and leaf count, shoot DW, and LUR. The root: shoot ratio and leaf reflectance were affected by DT and DIF. Positive DIF (DT higher than NT) caused darker-green leaf color than negative DIF. The node at which the first flower initiated was related to NT. The number of nodes to the first flower on pepper plugs grown at 26 C NT was 1.2 fewer than those of plants grown at 14 °C NT.


Author(s):  
Dwi Astutik ◽  
Ratih Rahhutami ◽  
Aline Sisi Handini ◽  
Ahmad Sutopo

<em>A planting medium that rich in nutrients is needed for oil palm, especially in the nursery phase. This study aimed to determine the effect of various doses of EM4 and to get the best dose of EM4 on the growth of oil palm seedlings in the pre-nursery. The research method used was a non-factorial completely randomized design with 4 treatments, namely 0 ml EM4 (A1), 4 ml EM4 (A2), 8 ml EM4 (A3), and 12 ml EM4 (A4). Each treatment was repeated 6 times so that there were 24 experimental units. The data obtained were analyzed for variance at the 5% level, if the effect was real, it was followed by the least significant difference (LSD) test. Based on the results of the study, it could be concluded that application of various EM4 doses had a significant effect on plant height (4-12 MST), the number of leaves (4-8 MST), trunk girth (4-12 MST), and shoot wet weight but did not significantly affect the number of leaves (12 MST), the volume of roots, length of roots, root wet weight and shoot dry weight, dry weight root, the best dose was shown at a dose of 12 ml EM4.</em>


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