DICLOFOP-METHYL AND DICLOFOP UPTAKE IN OAT (Avena sativa L.) PROTOPLASTS

1987 ◽  
Vol 67 (1) ◽  
pp. 215-223 ◽  
Author(s):  
SUSAN A. TRITTER ◽  
F. B. HOLL ◽  
BARRY G. TODD

An isolated oat (Avena sativa L.) protoplast system was used to investigate the uptake of the herbicide diclofop-methyl. Membrane permeability was not adversely affected by the herbicide levels and experimental conditions reported in this study. Neither diclofop-methyl nor diclofop adversely affected membrane permeability of the protoplasts at herbicide concentrations up to 50 μM and 100 μM, respectively. Diclofop-methyl was rapidly taken up by oat protoplasts to a constant level which did not change significantly with incubation times up to 1 h. Forty percent of the added 14C-diclofop-methyl was taken up within 5 s, while diclofop uptake was only 4% over the same time period. Diclofop-methyl uptake was linear with herbicide concentration in the uptake solution up to 50 μM. There was no significant difference between diclofop-methyl uptake by burst or intact protoplast preparations. Rapid diclofop-methyl uptake by oat protoplasts is apparently not a carrier-mediated process. Diclofop was taken up at very low rates under similar experimental conditions. These differences may reflect differences in the phytotoxic activity of the two chemical forms.Key words: Oat, protoplasts, diclofop-methyl uptake

Crisis ◽  
2005 ◽  
Vol 26 (1) ◽  
pp. 4-11 ◽  
Author(s):  
E. Kinyanda ◽  
H. Hjelmeland ◽  
S. Musisi

Abstract. Negative life events associated with deliberate self-harm (DSH) were investigated in an African context in Uganda. Patients admitted at three general hospitals in Kampala, Uganda were interviewed using a Luganda version (predominant language in the study area) of the European Parasuicide Study Interview Schedule I. The results of the life events and histories module are reported in this paper. The categories of negative life events in childhood that were significantly associated with DSH included those related to parents, significant others, personal events, and the total negative life events load in childhood. For the later-life time period, the negative life events load in the partner category and the total negative life events in this time period were associated with DSH. In the last-year time period, the negative life events load related to personal events and the total number of negative life events in this time period were associated with DSH. A statistically significant difference between the cases and controls for the total number of negative life events reported over the entire lifetime of the respondents was also observed, which suggests a dose effect of negative life events on DSH. Gender differences were also observed among the cases. In conclusion, life events appear to be an important factor in DSH in this cultural environment. The implication of these results for treatment and the future development of suicide interventions in this country are discussed.


1967 ◽  
Vol 06 (02) ◽  
pp. 45-51 ◽  
Author(s):  
A. Kent ◽  
J. Belzer ◽  
M. Kuhfeerst ◽  
E. D. Dym ◽  
D. L. Shirey ◽  
...  

An experiment is described which attempts to derive quantitative indicators regarding the potential relevance predictability of the intermediate stimuli used to represent documents in information retrieval systems. In effect, since the decision to peruse an entire document is often predicated upon the examination of one »level of processing« of the document (e.g., the citation and/or abstract), it became interesting to analyze the properties of what constitutes »relevance«. However, prior to such an analysis, an even more elementary step had to be made, namely, to determine what portions of a document should be examined.An evaluation of the ability of intermediate response products (IRPs), functioning as cues to the information content of full documents, to predict the relevance determination that would be subsequently made on these documents by motivated users of information retrieval systems, was made under controlled experimental conditions. The hypothesis that there might be other intermediate response products (selected extracts from the document, i.e., first paragraph, last paragraph, and the combination of first and last paragraph), that would be as representative of the full document as the traditional IRPs (citation and abstract) was tested systematically. The results showed that:1. there is no significant difference among the several IRP treatment groups on the number of cue evaluations of relevancy which match the subsequent user relevancy decision on the document;2. first and last paragraph combinations have consistently predicted relevancy to a higher degree than the other IRPs;3. abstracts were undistinguished as predictors; and4. the apparent high predictability rating for citations was not substantive.Some of these results are quite different than would be expected from previous work with unmotivated subjects.


2020 ◽  
Author(s):  
J Konzok ◽  
L Kreuzpointner ◽  
GI Henze ◽  
L Wagels ◽  
C Kärgel ◽  
...  

© 2020 Elsevier Inc. The Taylor Aggression Paradigm (TAP) is widely used to measure reactive aggression in laboratory settings. While modified versions (mTAPs) with various stimulus characteristics (shocks, noise, pressure, heat) have already been established, a modified version with monetary stimuli has only been introduced very recently. In this experiment, 209 young healthy participants (104 males, 105 females) completed a mock Competitive Reaction Time Task (CRTT) with a fictional opponent with preprogrammed 40 win and 60 lose trials. In lose trials, participants were provoked by subtracting a low (0–20 euro cents), medium (30–60 cents) or high (70–90 cents) amount of money from their fictitious account. Provocation stimuli were either presented randomly or in a fixed sequence (experimental conditions). In contrast to a random sequence, the fixed sequence was generated by repeating trials from the same provocation category in series of three. Linear mixed models (LMMs) considering aggression trajectories revealed significant effects of provocation (low, medium, high) and trait aggression (K-FAF) on reactive aggression. Men showed significantly higher reactive aggression levels than women. In regard to provocation sequence, we found no significant difference in reactive aggression between the random vs. fixed stimulus sequences. The findings provide new evidence supporting the view that the monetary mTAP is able to induce as well as capture reactive aggression in the laboratory. Additionally, we found no advantage of a fixed sequence as the level of reactive aggression in a given trial appeared to be mainly predicted by the preceding provocation trial.


2020 ◽  
Vol 35 (3) ◽  
Author(s):  
Tayyaba Gul Malik ◽  
Hina Nadeem ◽  
Eiman Ayesha ◽  
Rabail Alam

Objective: To study the effect of short-term use of oral contraceptive pills on intra-ocular pressures of women of childbearing age.   Methods: It was a comparative observational study, conducted at Arif memorial teaching hospital and Allied hospital Faisalabad for a period of six months. Hundred female subjects were divided into two groups of 50 each. Group A, included females, who had been taking oral contraceptive pills (OCP) for more than 6 months and less than 36 months. Group B, included 50 age-matched controls, who had never used OCP. Ophthalmic and systemic history was taken. Careful Slit lamp examination was performed and intraocular pressures (IOP) were measured using Goldman Applanation tonometer. Fundus examination was done to rule out any posterior segment disease. After collection of data, we analyzed and compared the intra ocular pressures between the two groups by using ANOVA in SPSS version 21.   Results: Average duration of using OCP was 14.9 months. There was no significant difference of Cup to Disc ratios between the two groups (p= 0.109). However, significant difference was noted between the IOP of OCP group and controls. (p=0.000). Conclusion: OCP significantly increase IOP even when used for short time period.


Author(s):  
Chang Park ◽  
Kapil Sugand ◽  
Arash Aframian ◽  
Catrin Morgan ◽  
Nadia Pakroo ◽  
...  

Abstract Introduction COVID-19 has been recognized as the unprecedented global health crisis in modern times. The purpose of this study was to assess the impact of COVID-19 on treatment of neck of femur fractures (NOFF) against the current guidelines and meeting best practice key performance indicators (KPIs) according to the National Hip Fracture Database (NHFD) in two large central London hospitals. Materials and methods A multi-center, longitudinal, retrospective, observational study of NOFF patients was performed for the first ‘golden’ month following the lockdown measures introduced in mid-March 2020. This was compared to the same time period in 2019. Results A total of 78 cases were observed. NOFFs accounted for 11% more of all acute referrals during the COVID era. There were fewer overall breaches in KPIs in time to theatre in 2020 and also for those awaiting an orthogeriatric review. Time to discharge from the trust during the pandemic was improved by 54% (p < 0.00001) but patients were 51% less likely to return to their usual residence (p = 0.007). The odds ratio was significantly higher for consultant surgeon-led operations and consultant orthogeriatric-led review in the post-COVID era. There was no significant difference in using aerosol-generating anaesthetic procedures or immortality rates between both years. Conclusion The impact of COVID-19 pandemic has not adversely affected the KPIs for the treatment of NOFF patients with significant improvement in numerous care domains. These findings may represent the efforts to ensure that these vulnerable patients are treated promptly to minimize their risks from the coronavirus.


Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1272
Author(s):  
Judit Tajti ◽  
Magda Pál ◽  
Tibor Janda

Oat (Avena sativa L.) is a widely cultivated cereal with high nutritional value and it is grown mainly in temperate regions. The number of studies dealing with gene expression changes in oat continues to increase, and to obtain reliable RT-qPCR results it is essential to establish and use reference genes with the least possible influence caused by experimental conditions. However, no detailed study has been conducted on reference genes in different tissues of oat under diverse abiotic stress conditions. In our work, nine candidate reference genes (ACT, TUB, CYP, GAPD, UBC, EF1, TBP, ADPR, PGD) were chosen and analysed by four statistical methods (GeNorm, Normfinder, BestKeeper, RefFinder). Samples were taken from two tissues (leaves and roots) of 13-day-old oat plants exposed to five abiotic stresses (drought, salt, heavy metal, low and high temperatures). ADPR was the top-rated reference gene for all samples, while different genes proved to be the most stable depending on tissue type and treatment combinations. TUB and EF1 were most affected by the treatments in general. Validation of reference genes was carried out by PAL expression analysis, which further confirmed their reliability. These results can contribute to reliable gene expression studies for future research in cultivated oat.


Author(s):  
Cosmin Miha Moca ◽  
Dan Mihai Gherţoiu

ABSTRACT. Introduction. Reaction is a purposeful voluntary response to an external stimulus. There is certain time period between application of external stimulus and appropriate motor response to the stimulus called the reaction time. Objectives. The aim of this paper was to determine if different colour contrasts affects the reaction time of young tennis players. Materials and Methods. The participants in this study were young tennis players (N = 10), 3 females and 7 males, with the ages between 12 to 13 years old. Results. There was a significant difference in the scores for white background (M=7.5, SD=1.51) and orange background (M=6, SD=0.81) conditions; t(9)=3.30, p = 0.009. Conclusion. Our study managed to show that a different kind of background colour can affect the reaction accuracy in identifying an object of different shape and colour than the background.


2008 ◽  
Vol 2 (2) ◽  
Author(s):  
Glenn Nordehn ◽  
Spencer Strunic ◽  
Tom Soldner ◽  
Nicholas Karlisch ◽  
Ian Kramer ◽  
...  

Introduction: Cardiac auscultation accuracy is poor: 20% to 40%. Audio-only of 500 heart sounds cycles over a short time period significantly improved auscultation scores. Hypothesis: adding visual information to an audio-only format, significantly (p<.05) improves short and long term accuracy. Methods: Pre-test: Twenty-two 1st and 2nd year medical student participants took an audio-only pre-test. Seven students comprising our audio-only training cohort heard audio-only, of 500 heart sound repetitions. 15 students comprising our paired visual with audio cohort heard and simultaneously watched video spectrograms of the heart sounds. Immediately after trainings, both cohorts took audio-only post-tests; the visual with audio cohort also took a visual with audio post-test, a test providing audio with simultaneous video spectrograms. All tests were repeated in six months. Results: All tests given immediately after trainings showed significant improvement with no significant difference between the cohorts. Six months later neither cohorts maintained significant improvement on audio-only post-tests. Six months later the visual with audio cohort maintained significant improvement (p<.05) on the visual with audio post-test. Conclusions: Audio retention of heart sound recognition is not maintained if: trained using audio-only; or, trained using visual with audio. Providing visual with audio in training and testing allows retention of auscultation accuracy. Devices providing visual information during auscultation could prove beneficial.


2007 ◽  
Vol 21 (3) ◽  
pp. 253-258 ◽  
Author(s):  
Tomie Nakakuki Campos ◽  
Cleber Henrique Inoue ◽  
Elcio Yamamoto ◽  
Ângela Toshie Araki ◽  
Lena Katekawa Adachi ◽  
...  

There are situations in which intraradicular retainers have to be removed and replaced. The objective of this research was to evaluate the apical seal after the removal of a custom cast post and core with a carbide bur or with an ultrasound apparatus. Twenty five roots of extracted human incisors were used. They were endodontically treated and prepared to receive the posts. The posts and cores were cast with 2 types of dental alloys, CuAlZn and PdAg, and were cemented with zinc phosphate cement. After 24 hours, they were removed using the two above mentioned techniques. Then, the roots had their external surface made impermeable by two layers of cyanoacrylate adhesive, leaving only the cervical area for dye penetration. The teeth were immersed in rhodamine for 24 hours. They were then cut and observed under an optical microscope and analyzed with appropriate software (Imagelab). The results were submitted to ANOVA, and they evidenced that, regarding the alloy factor, PdAg posts presented a larger mean infiltration value (2.23 ± 0.48 mm) as compared to the posts made of CuAlZn (1.39 ± 0.48 mm) (p = 0.025). Regarding the technique factor, there was no significant difference (p = 0.9) between the removal of the intraradicular retainer using ultrasound (1.99 ± 0.62 mm) or using a rotating cutting instrument (1.62 ± 0.62 mm). Under these experimental conditions, it was possible to conclude that the degree of apical leakage was directly related to the alloy type, and it was present in both techniques used.


2018 ◽  
Vol 51 (4) ◽  
pp. 173
Author(s):  
Vita Previa Indirayana ◽  
Gita Gayatri ◽  
N. R. Yuliawati Zenab

Background: Model analysis constitutes an essential aspect of orthodontic diagnostic practice. Pavan has developed an application to simplify the mathematical calculations employed in orthodontic model analysis. Purpose: This study was conducted to obtain the differences in results and time periods of model analysis using conventional means and iModelAnalysis. Methods: The research represented a comparative analytic study. The populations comprised dental casts dating from 2014 in the Orthodontics Laboratory of Padjadjaran University. The samples comprised 31 dental casts which were subjected to a total sampling method consisting of two treatments; a conventional method calculation and one using iModelAnalysis. A normality test was conducted and processed using a paired t-test with α=0.05. Results: The means of arch length discrepancies were 1.64±2.63 mm and 1.37±3.07 mm for the conventional methods and 1.65±2.43mm and 1.42±3.04mm for iModelAnalysis. The results of a Bolton analysis for conventional methods were 78.05±2.69% and 91.93±1.29%, while those for iModelAnalysis were 77.91±2.70% and 91.96±2.13%. A Howes analysis of conventional methods produced a result of 45.56±2.83%, while for an iModelAnalysis one of 45.56±2.85%. Pont analysis for conventional methods was 39.35±0.04 mm and 49.17±2.55 mm, while for iModelAnalysis it was 39.35±0.07 mm and 49.19±2.57mm. The mean of the duration of analysis using conventional methods was 1703.81±56.46 seconds, while for iModelAnalysis it was 990.06±34.87 seconds. A normality test confirmed that the data was normally distributed (p>0.05). The results of a paired sample t-test with p>0.05 showed that there was no significant difference between the results of each analysis, while there was significant difference in the time period of analysis. Conclusion: There was no difference in the analysis results. However, there was difference in the time period of analysis between conventional methods and that of iModelAnalysis.


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