COMPARISON OF THE HAGBERG FALLING NUMBER AND MODIFIED GRAIN AMYLASE ANALYZER METHODS FOR ESTIMATING SPROUT DAMAGE IN RYE
The Hagberg falling number method and a recently developed turbidometric method using the Perkin Elmer Model 191 Grain Amylase Analyzer were evaluated for measuring the levels of α-amylase activity in rye samples with varying sprout damage. Poor correlations were obtained between the a-amylase methods and percent visual sprout damage, although the two α-amylase methods correlated fairly well with each other. Differences between levels of α-amylase activity and levels of sprout damage may be explained by: (a) the difficulty in assessing sprout damage levels due to the presence of degermed or skinned kernels; (b) the association of higher levels of α-amylase activity with other types of damaged kernels; and (c) the severity of sprouting of individual rye kernels.