STARCH GEL ELECTROPHORESIS TECHNIQUE USED WITH ALFALFA AND OTHER Medicago SPECIES

1981 ◽  
Vol 61 (3) ◽  
pp. 745-749 ◽  
Author(s):  
CARLOS F. QUIROS
Keyword(s):  
Enzyme Activity ◽  
Related Species ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Alcohol Dehydrogenases ◽  
Leucine Aminopeptidases ◽  
Starch Gel ◽  
Medicago Sativa L ◽  
Medicago Species ◽  
Electrophoresis Technique

Starch gel electrophoresis is currently used in our laboratory for the study of the genetics and evolution of alfalfa (Medicago sativa L.) and related species. An account is given of this technique, and the staining of the gels for enzyme activity of peroxidases, leucine-aminopeptidases, esterases, and alcohol dehydrogenases.

scholarly journals Erythrocyte glutathione S-transferase. Electrophoretic identification of two enzyme forms

1983 ◽  
Vol 215 (1) ◽  
pp. 213-216 ◽  
Author(s):  
R C Strange ◽  
P H Hirrell ◽  
G A Kitley ◽  
D A Hopkinson ◽  
W Cotton
Keyword(s):  
Enzyme Activity ◽  
Individual Differences ◽  
Gel Electrophoresis ◽  
Human Erythrocytes ◽  
Starch Gel Electrophoresis ◽  
Glutathione S Transferase ◽  
Electrophoretic Patterns ◽  
Starch Gel

Starch-gel electrophoresis was used to demonstrate two forms of glutathione S-transferase in human erythrocytes. Whereas considerable inter-individual differences in enzyme activity and electrophoretic patterns were detected, intra-individual differences were small.

CONGENITAL NONSPHEROCYTIC HEMOLYTIC ANEMIA ASSOCIATED WITH ERYTHROCYTE GLUCOSE-6-PHOSPHATE DEHYDROGENASE DEFICIENCY IN A NEGRO FAMILY

PEDIATRICS ◽  
1966 ◽  
Vol 37 (4) ◽  
pp. 624-629
Author(s):  
Aaron Grossman ◽  
K. Ramanathan ◽  
Parvin Justice ◽  
Joan Gordon ◽  
Nasrollah T. Shahidi ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Hemolytic Anemia ◽  
Gel Electrophoresis ◽  
Dehydrogenase Deficiency ◽  
Red Cells ◽  
Starch Gel Electrophoresis ◽  
Negro Family ◽  
Starch Gel ◽  
Normal Enzyme ◽  
Glucose 6 Phosphate Dehydrogenase

This paper describes a Negro family with congenital nonspherocytic hemolytic anemia associated with glucose-6-phosphate dehydrogenase deficiency. All four affected males in this family showed a hemolytic anemia characterized by low hemoglobin, reticulocytosis, and jaundice. There was no detectable G-6-P D in the red cells and about a tenth of normal enzyme activity in the white cells. By starch gel electrophoresis, the G-6-P D was present as a single band which migrated at the rate of 104% of normal. Physico-chemical studies revealed a marked increase of the Michaelis constant for both G-6-P and TPN, a marked lability of the enzyme upon heating at 40°C and 48°C, and a single narrow peak at pH 9.0. Most of these features were similar to those seen in the Oklahoma I variant. Four of the females in the family were studied (two sisters, the mother, and a maternal aunt of the propositus); all showed a lesser degree of anemia and reticulocytosis but no jaundice, except in the mother. There was a decrease of haptoglobins and both the mother and one sister showed a decrease of erythrocyte survival time as measured by chromium-51. The female members had between 11 and 26% of normal G-6-P D activity in the red cells and between 35 and 63% of normal enzyme activity in the white cells. Starch gel electrophoresis in the mother and aunt showed a single band which migrated at 110% of normal with a trail at the position of the affected males. The presence of a milder degree of hemolysis in the heterozygous carriers of the gene for G-6-P D deficiency associated with congenital nonspherocytic hemolytic anemia provides further support for the Lyon hypothesis in man.

Improving of Starch Gel Electrophoresis Technique by Gamma Irradiation

1977 ◽  
Vol 29 (5) ◽  
pp. 157-160 ◽  
Author(s):  
R. M. A. El Saadany ◽  
F. M. El Saadany ◽  
Y. H. Foda
Keyword(s):  
Gamma Irradiation ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Starch Gel ◽  
Electrophoresis Technique

Electrophoretic studies on enzymes of diploid and triploid paragonimus westermani

Parasitology ◽  
1985 ◽  
Vol 91 (3) ◽  
pp. 489-497 ◽  
Author(s):  
Takeshi Agatsuma ◽  
Shigehisa Habe
Keyword(s):  
Related Species ◽  
Gel Electrophoresis ◽  
Enzyme Analysis ◽  
Single Mutation ◽  
Starch Gel Electrophoresis ◽  
Glutamic Oxaloacetic Transaminase ◽  
Closely Related Species ◽  
Paragonimus Westermani ◽  
Starch Gel ◽  
Triploid Form

SUMMARYAn enzyme analysis of diploid and triploid Paragonimus westermani was conducted using starch gel electrophoresis. In total, 16 enzymes, probably encoded by 18 loci, were studied for 3 populations of the diploid form sampled from 2 localities, and 4 populations of the triploid form from 4 localities. Comparison of the enzymes of the triploid and the diploid digeneans showed 5 different patterns; diaphorase (EC 1.6.2.2), glutamic-oxaloacetic transaminase (EC 2.6.1.1), hexokinase (EC 2.7.1.1), leucylglycylglycine aminopeptidase (EC 3.4.1.3), and phosphoglucomutase (EC 2.7.5.1). On the basis of the numbers of bands and their patterns, all individuals of the triploid are probably heterozygous at each of these 5 loci and homozygous at the remaining 13 loci. The occurrence of fixed heterozygotes found in triploid populations cannot be easily explained by only a single mutation. It is suggested that the variability may have been introduced by hybridization with a different sub-species or a closely related species and may, thus, have been maintained since the time of the origin of triploids.

Sinapine Esterase. II . Specificity and Change of Sinapine Esterase Activity During Germination of Raphanus sativus

1980 ◽  
Vol 35 (11-12) ◽  
pp. 963-966 ◽  
Author(s):  
Dieter Strack ◽  
Gerhild Nurmann ◽  
Gesine Sachs
Keyword(s):  
Enzyme Activity ◽  
Gel Electrophoresis ◽  
Esterase Activity ◽  
Raphanus Sativus ◽  
Lag Phase ◽  
Starch Gel Electrophoresis ◽  
Rapid Degradation ◽  
Choline Esters ◽  
Starch Gel ◽  
Naphthyl Acetate

Abstract Following a 20 to 24 h lag-phase after sowing, the onset of both rapid degradation of sinapine (sinapolycholine) and rapid increase in sinapine esterase activity in cotyledons of Raphanus sativus was observed. After 2 days of germination maximal enzyme activity was reached and declined in subsequent germination stages as rapidly as it had appeared. Esterases, active against indophenyl acetate, showed highest activity in dry seeds, declining to more than 50% between the 1st and 3rd day of germination. Starch gel electrophoresis showed that all protein extracts contained a multiplicity of esterases, active against α-naphthyl acetate. When gels were incubated with sinapine, one new band appeared, stainable with diazotized ρ-nitroaniline. This band represents sinapine esterase activity. Tests for substrate specificity towards cinnamic acid choline esters showed highest enzyme activity with sinapine. Studies on the occurrence of sinapine esterase in other Brassicaceae revealed that the enzyme activity coincides with the occurrence and degradation of sinapine.

scholarly journals GENETIC POLYMORPHISM AND EVOLUTION IN PARTHENOGENETIC ANIMALS. I. POLYPLOID CURCULIONIDAE

Genetics ◽  
1973 ◽  
Vol 74 (3) ◽  
pp. 489-508
Author(s):  
Esko Suomalainen ◽  
Anssi Saura
Keyword(s):  
Genetic Polymorphism ◽  
Genetic Variability ◽  
Related Species ◽  
Gel Electrophoresis ◽  
Hybrid Origin ◽  
Starch Gel Electrophoresis ◽  
Closely Related Species ◽  
Hardy Weinberg Equilibrium ◽  
Starch Gel ◽  
Different Populations

ABSTRACT The genetic variability at enzyme loci in different triploid and tetraploid parthenogenetic weevil populations has been elucidated by starch gel electrophoresis. The overall genotype of individual weevils belonging to different populations has been determined for over 25 loci. The results are compared with those obtained for diploid bisexual races of either the same or closely related species. The variation within a parthenogenetic population differs from that in diploid, sexually reproducing populations, i.e. the allele frequencies are not in a Hardy-Weinberg equilibrium. The results indicate that apomictic parthenogenetic populations can differentiate genetically. The genotypes within a population resemble each other more than genotypes belonging to different populations. It is evident that evolution still continues-even if slowed down—in parthenogenetic weevils. A comparison between the allele relationships in geographically isolated polyploid parthenogenetic populations and related diploid bisexual forms does not support the hypothetical hybrid origin of parthenogenesis and polyploidy in weevils. Parthenogenesis within a parthenogenetic weevil species is evidently monophyletic.

Fractionation of Plasminogen by Starch Gel Electrophoresis

1964 ◽  
Vol 12 (01) ◽  
pp. 126-136 ◽  
Author(s):  
Karl H. Slotta ◽  
J. D Gonzalez
Keyword(s):  
Gel Electrophoresis ◽  
Room Temperature ◽  
Broad Band ◽  
Caproic Acid ◽  
Starch Gel Electrophoresis ◽  
Full Activity ◽  
Veronal Buffer ◽  
Starch Gel ◽  
Alkaline Range

SummaryWhen urea or ε-amino caproic acid were used as solublizing agents for plasminogen in electrophoretic experiments, only one broad band of the proenzyme was obtained on acetate cellulose, in starch block, and in acrylamide gel. In starch gel electrophoresis, however, both forms of plasminogen – the native or euglobulin and Kline’s or Pseudoglobulin plasminogen – separated into six bands. These migrated toward the cathode at room temperature in borate or veronal buffer in the alkaline range and showed full activity in fibrinagar-streptokinase plates.

Sign in / Sign up

Export Citation Format

Share Document