IN VITRO PROPAGATION OF SPIREA BUMALDA AND PRUNUS CISTENA FROM SHOOT APICES

1979 ◽  
Vol 59 (4) ◽  
pp. 1025-1029 ◽  
Author(s):  
W. DAVID LANE
Keyword(s):  
In Vitro Propagation ◽  
Multiple Shoot ◽  
Shoot Cultures ◽  
Ms Medium ◽  
Liquid Media ◽  
Shoot Apices ◽  
Large Numbers ◽  
Shoot Production ◽  
Multiple Shoot Cultures

A procedure was developed to produce large numbers of plants from multiple shoot, cultures which were obtained from shoot apices 1–5 mm long of Spirea bumalda ’Anthony Waterer’ and Prunus cistena grown on Murashige and Skoog (MS) medium containing 5 μM benzyladenine. Shoot production, harvested every 3–4 wk, yielded 300–400 shoots of Spirea and approximately 35 of Prunus per cycle. The salt concentration of MS inhibited rooting was reduced by half. Liquid media usually resulted in greater percent rooting than agar. Although there was some rooting of both species in medium without naphthalenacetic acid, this auxin increased rooting of both species with optima at about 0.1 μM for Spirea and 1 μM for Prunus.

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals In vitro propagation of muskmelon (Cucumis melo L.) from nodal segments, shoot tips and cotyledonary nodes

2015 ◽  
Vol 41 ◽  
pp. 71-77
Author(s):  
S. Parvin ◽  
M. Kausar ◽  
M. Enamul Haque ◽  
M. Khalekuzzaman ◽  
B. Sikdar ◽  
...  
Keyword(s):  
Cucumis Melo ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Ms Medium ◽  
Cotyledonary Nodes ◽  
Cucumis Melo L

A rapid and efficient protocol is outlined for in vitro propagation of muskmelon(Cucumis melo L.) Shoot tips, nodal segments and cotyledonary nodes from invitro grown seedlings were used as explants. The explants were inoculated on MS medium fortified with different combinations and concentrations of growthregulators viz., BAP, NAA, GA3 and IBA for multiple shoot regeneration.Effective result was found on MS medium supplemented with 2.0 mg/l BAP, inwhich 90% and 70% cultures induced multiple shoots from nodal segments andshoot tip explants, respectively. Whereas, 70% cultures of cotyledonary nodeswere found to induced shoots on MS medium with 1.5 mg/l BAP + 0.1 mg/l GA3. In vitro regenerated shoots were subcultured on half strength MS mediumsupplemented with different concentrations of IBA and NAA for successful rootinduction and the effective result (up to 70%) was found in medium with 1 mg/lIBA. Well rooted in vitro grown plantlets were acclimatized in sandy soil, whereas 70% plantlets survived

scholarly journals Multiple Shoot Production In Vitro of the Tropical Timber Tree, Sentang (Azadirachta excelsa Linn.)

HortScience ◽  
1998 ◽  
Vol 33 (6) ◽  
pp. 1073-1075 ◽  
Author(s):  
T.K. Liew ◽  
C.K.H. Teo
Keyword(s):  
Sodium Hypochlorite ◽  
Multiple Shoot ◽  
Axillary Buds ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Tropical Timber ◽  
Shoot Production ◽  
Timber Tree

Axillary buds from 5-month-old seedlings of Azadirachta excelsa Linn. were surface-sterilized twice with 1.35% (m/v) and 1.05% (m/v) of sodium hypochlorite for 25 and 15 minutes, respectively, before culturing on Murashige and Skoog (MS) medium containing combinations of BA and NAA. A combination of 4.4 μM BA + 0.5 μM NAA induced the most axillary buds to grow (eight per explant). Subsequent proliferation of the micropropagated shoots on this medium yielded abnormal shoots. The best medium for maximum proliferation of these micropropagated shoots contained 3.3 μM BA and 0.27 μM NAA. On this medium about four normal shoots were produced per explant. These findings indicate that two different media are needed for successful micropropagation of sentang. Chemical names used: N6-benzylaminopurine (BA); 1-naphthaleneacetic acid (NAA).

scholarly journals Glycowithanolides accumulation in in Vitro Shoot Cultures of Indian Ginseng (Withania somnifera Dunal)

2009 ◽  
Vol 4 (4) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ashok Ahuja ◽  
Devinder Kaur ◽  
Mallubhotla Sharada ◽  
Arun Kumar ◽  
Krishan Avtar Suri ◽  
...  
Keyword(s):  
Spectroscopic Data ◽  
Withania Somnifera ◽  
Renewable Resource ◽  
Multiple Shoot ◽  
Biologically Active ◽  
Shoot Cultures ◽  
Indian Ginseng ◽  
Multiple Shoot Cultures ◽  
In Vitro Shoot Cultures

Phytochemical investigations of multiple shoot cultures of selected accessions AGB002 and AGB025 of Withania somnifera. established in vitro utilizing shoot tip apices cultured on Murashige and Skoog's medium supplemented with BAP (1 mg/L) have been carried out. This has lead to isolation of four glycowithanolides viz. Withanoside IV (WSG-3), Withanoside VI (WSG-3A), Physagulin D (WSG-P) and Withastraronolide (WSC-O). The structures of these have been confirmed on the basis of spectroscopic data. Multiple shoot cultures could be an alternative renewable resource for production of these biologically active molecules.

scholarly journals Tissue culture of Ophiorrhiza mungos L., a prospective method for the production of an anticancer drug, camptothecin

2018 ◽  
Vol 5 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Geethu Gopinath ◽  
Binoy Jose ◽  
P Ravichandran ◽  
K Satheeshkumar
Keyword(s):  
Indole Alkaloid ◽  
Multiple Shoot ◽  
Shoot Cultures ◽  
Solid Media ◽  
Half Strength ◽  
Multiple Shoot Cultures ◽  
Trade Names ◽  
Approved Drugs ◽  
Rooted Shoot

Camptothecin (CPT), a cytotoxic quinoline indole alkaloid, is an anticancer compound. Its two major semi synthetic derivatives, topotecan and irinotecan, are Food and Drug Administration (FDA) approved drugs effectively used for treating different cancer types and sold under the trade names Hycamtin and Camptosar. Among the Ophiorrhiza species, Ophiorrhiza mungos contains the highest CPT level (0.02 % g dw). CPT level was determined in plants before flowering (0.074 ± 0.003 % g dw) and at flowering (0.052 ± 0.002 % g dw). Multiple shoot cultures were induced on seedling-derived explants of O. mungos in half strength MS solid media supplemented with 1.0 mg/L BAP to obtain 12.00 ± 1.22 shoots in 20 days. Repeated subcultures at 20 days interval yielded 20.00 ± 3.71 shoots/subculture of shoot clusters. After elongation, rooting and transplanting, the growth of shoot clusters were studied in both in vitro and same aged naturally grown seedlings. Highest biomass (4.62 ± 0.158 g fw) was obtained in in vitro-derived shoot clusters. CPT increased according to biomass and the maximum CPT was recorded in in vitro rooted shoot clusters after 15 days (0.031 ± 0.001 % g dw). Hence, in vitro-derived rooted shoot clusters of O. mungos cultivated in net-pots for 60 days under shade net house conditions found to be a sustainable source for CPT.

scholarly journals Micropropagation of Yellow Passionfruit by Axillary Bud Proliferation

HortScience ◽  
1997 ◽  
Vol 32 (7) ◽  
pp. 1276-1277 ◽  
Author(s):  
Joao L.C. Faria ◽  
Juan Segura
Keyword(s):  
Growth Regulators ◽  
In Vitro Propagation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Shoot Apices ◽  
Passiflora Edulis ◽  
Multiple Shoot Formation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium

A protocol for in vitro propagation in yellow passionfruit (Passiflora edulis F. flavicarpa Deg) has been developed. Shoot apices from aseptically grown seedlings were used as initial explants. Multiple shoot formation was obtained by placing the explants on solidified Murashige and Skoog medium containing BA. Regenerated shoots were rooted on media without growth regulators. Following conventional procedures, plantlets were transferred to soil with more than 90% success. Chemical name used: N-(phenylmethyl)-lH-purin-6-amine (BA).

scholarly journals In Vitro Propagation and Somatic Embryogenesis in Phalsa

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1104A-1104
Author(s):  
Bipul Biswas ◽  
Nirmal Joshee ◽  
Ashish Yadav ◽  
Anand K. Yadav
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Propagation ◽  
Cold Hardiness ◽  
Basal Medium ◽  
Multiple Shoot ◽  
Zygotic Embryos ◽  
Ms Medium ◽  
Mature Trees ◽  
Apply Biotechnology

Phalsa[Grewia asiatica (L.) Tiliaceae] is an exotic fruit with good nutraceutical values. It cannot be grown in temperate climates with severe winters. Therefore, genetic improvement of phalsa for cold tolerance is essential. In order to apply biotechnology through genetic transformation to enhance cold hardiness, a reliable and rapid micropropagation system is needed. Thus, developing the most dependable micropropagation protocols for phalsa was the primary goal of this research. Phalsa explants prepared from different tissues, including leaf, nodes, internode, and zygotic embryos, were collected from mature trees growing in the specialty plants house, cultured on MS medium supplemented with various cytokinins alone or along with auxins and incubated under a 10-hour photoperiod at ambient temperature. In vitro propagation of phalsa tissues through both organogenesis and somatic embryogenesis was achieved. Of these, single shoots were developed from nodal explants as a result of budbreak on MS medium supplemented with BAP, kinetin, and zeatin separately. Somatic embryos were developed from the zygotic embryos when cultured on MS medium with 0.023 μm BA + 0.022 μm zeatin, for 2 weeks following a pulse treatment on NN medium supplemented with 5% sucrose, 0.11 μm BAP, 0.22 μm 2,4-D, and 29.20 μm L-glutamine. Somatic embryogenesis was also observed on modified basal medium supplemented with 13% sucrose, 58.40 μm L-glutamine, and 1.75 μm IAA. Enormous callusing was a major problem for in vitro studies with this species, irrespective of media composition. Further studies for multiple shoot development and higher frequency of SE induction are under way.

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