TRANSMISSION ELECTRON MICROSCOPY OF FUNGAL NEMATODE-TRAPPING DEVICES

1979 ◽  
Vol 59 (3) ◽  
pp. 785-795 ◽  
Author(s):  
S. S. TZEAN ◽  
R. H. ESTEY
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cytoplasmic Organelles ◽  
Dense Bodies ◽  
Transmission Electron ◽  
Luminal Side ◽  
Septal Pores ◽  
Trapping Devices

The nematode-trapping devices of Arthrobotrys dactyloides (constricting rings), Monacrosporium cionopagum (adhesive columnar processes and scalariform loops) and a Dactylella sp. (sticky knobs) were investigated by electron microscopy. The cells of the constricting rings prior to inflation contained normal cytoplasmic organelles and some unusual, oblong, electron-dense inclusions in the luminal side of the protoplast, and lomasomes associated with papillate cylindrical bodies in the peripheral side. Their luminal walls differed from their peripheral walls in structure and thickness. After inflation, the ring cells had thinner luminal walls, the electron-dense inclusions were absent, there were fewer lomasomes, the cells had larger vacuoles, some of which contained electron-dense fine granules, and Woronin bodies were plugging the septal pores. It is postulated that the cells of constricting rings are inflated by means of rapidly generated gases rather than by an inflow of fluids. The sticky knob, adhesive columnar process, and scalariform loop trapping devices exhibited numerous globose electron-dense bodies, especially in their peripheral protoplasts.

scholarly journals Effect of Electrocautery on Endothelial Integrity of the Internal Thoracic Artery: Ultrastructural Analysis with Transmission Electron Microscopy

2014 ◽  
Vol 41 (5) ◽  
pp. 484-490 ◽  
Author(s):  
Burak Onan ◽  
Mehmet Yeniterzi ◽  
Ismihan Selen Onan ◽  
Burak Ersoy ◽  
Suheyla Gonca ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Endothelial Cells ◽  
Internal Thoracic Artery ◽  
Ultrastructural Changes ◽  
Control Group ◽  
Elastic Tissue ◽  
Cytoplasmic Organelles ◽  
Transmission Electron ◽  
Sharp Dissection

The internal thoracic artery (ITA) is typically harvested from the chest wall by means of conventional electrocautery. We investigated the effects of electrocautery on endothelial-cell and vessel-wall morphology at the ultrastructural level during ITA harvesting. Internal thoracic artery specimens from 20 patients who underwent elective coronary artery bypass grafting were investigated in 2 groups. The ITA grafts were sharply dissected with use of a scalpel and clips in the control group (n=10) and were harvested by means of electrocautery in the study group (n=10). Each sample was evaluated for intimal, elastic-tissue, muscular-layer, and adventitial changes. Free flow was measured intraoperatively. Light microscopic examinations were performed after hematoxylin-eosin and Masson's trichrome staining. Transmission electron microscopy was used to evaluate ultrastructural changes in the endothelial cells and vessel walls of each ITA. In the sharp-dissection group, the endothelial surfaces were lined with normal amounts of original endothelium, endothelial cells were distinctly attached to the basal lamina, cytoplasmic organelles were evident, and intercellular junctional complexes were intact. Conversely, in the electrocautery group, the morphologic integrity of endothelial cells was distorted, with some cell separations and splits, contracted cells, numerous large cytoplasmic vacuoles, and no visible cytoplasmic organelles. The subendothelial layer exhibited disintegration. Free ITA flow was higher in the sharp-dissection group (P=0.04). The integrity of endothelial cells can be better preserved when the ITA is mobilized by means of sharp dissection, rather than solely by electrocautery; we recommend a combined approach.

Scanning and transmission electron microscopy of the female reproductive system of Schistosoma margrebowiei Le Roux, 1933

1990 ◽  
Vol 64 (3) ◽  
pp. 181-192 ◽  
Author(s):  
A. H. H. Awad ◽  
A. J. Probert
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Secretory Cell ◽  
Single Type ◽  
Secretory Cells ◽  
Female Reproductive System ◽  
Sense Organs ◽  
Vitelline Duct ◽  
Dense Bodies ◽  
Transmission Electron

ABSTRACTTransmission electron microscopy shows that the uterus of female Schistosoma margrebowiei possesses the same ultrastructure as that of the tegument but lacks spines and sense organs. It does not possess secretory cells and opens at the gonopore which by scanning electron microscopy was seen to be composed of numerous leaf-like protrusions. The morphology of the ovary is comparable with that of other Digenea. Immature and mature ova possess cortically arranged granules and occur within the posterior zone of the ovary. Cilia and lamellae line the luminal surface of the oviduct and ootype, the lamellae running unidirectionally along the duct. Only a single type of secretory cell is seen within Mehlis' gland and this produces dense bodies which are associated with Goldi bodies. Narrow cytoplasmic channels supported by microtubules deliver these secretory bodies to the ootype. The vitelline duct is lined with cilia and lamellae and the vitelline gland contains four types of cells, S1, S2, S3 and S4. Calcareous corpuscles are found within mature S4 cells.

scholarly journals Spermatogenesis and Spermiogenesis of the Aphanius Dispar

1999 ◽  
Vol 4 ◽  
pp. 1
Author(s):  
Taher A. Ba-Omar
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Germ Cells ◽  
Lateral Side ◽  
Ultra Structure ◽  
Cytoplasmic Organelles ◽  
Transmission Electron ◽  
Almost All ◽  
Cystic Type

Light and transmission electron microscopy were used to study the structure of various stages of germ cells of the Aphanius dispar (Ruppell l828) in Oman. A dispar testes were of cystic type. Different stages of spermatogonia, spermatocytes and spermatids were observed within the cysts. Each cyst is occupied by a group of germ cells almost all in the same stage. The morphology and ultra structure of the nuclei and cytoplasmic organelles of the germ cells are described. Some flagella possessing lateral side fins and some biflagellated spermatozoa were seen.

Fine structure and development of the zygospore of Rhizopus sexualis (Smith) Callen

1971 ◽  
Vol 263 (848) ◽  
pp. 71-100 ◽  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Probable Function ◽  
Cytoplasmic Organelles ◽  
Transmission Electron ◽  
Scanning Electron ◽  
Number Form

The development of the zygospore of Rhizopus sexualis (Smith) Callen was followed by light microscopy, transmission electron microscopy and scanning electron microscopy. Details of all stages, including dissolution of the fusion wall, delimitation of the gametangia by septa, and formation of the complex wall of the zygospore are described and illustrated. Changes in number, form and distribution of the organelles and the behaviour of the nuclei are described. The probable function of the cytoplasmic organelles and the possible mechanisms controlling development are discussed.

Ultrastructure of stromatal anamorphs in the Sclerotiniaceae

1989 ◽  
Vol 67 (2) ◽  
pp. 394-406 ◽  
Author(s):  
Linda M. Kohn ◽  
Douglas J. Grenville
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Walls ◽  
Monilinia Fructicola ◽  
Cellular Organization ◽  
Ultrastructural Studies ◽  
Sclerotium Cepivorum ◽  
Transmission Electron ◽  
Septal Pores

As part of comparative anatomical, histochemical, and ultrastructural studies of stromata in the Sclerotiniaceae, mature stromata produced in vitro by 11 species representing six genera and one form-genus were examined using transmission electron microscopy. Sclerotial-stromatal taxa were Sclerotinia sclerotiorum, S. trifoliorum, S. minor, Sclerotium cepivorum, Botrytis cinerea, B. porri, Monilinia fructicola, and Myriosclerotinia borealis. Substratal-stromatal taxa were Sclerotinia homoeo-carpa, Rutstroemia sydowiana, and Lambertella subrenispora. Three types of rind were observed: a living cellular rind, a dead cellular rind, and a stromatal rind. Sclerotial species were distinguished from stromatal species not only by the rind type, but also by the confluent extracellular matrix around cortical and medullary cell walls. Presence of lacunae in this matrix distinguished Sclerotinia spp. and M. borealis from Botrytis spp. and Monilinia fructicola. Rind, cortical, and medullary cells contained abundant storage vacuoles in most taxa. The distribution and proportion of organelles to storage vacuoles differed among taxa. Plugged septal pores with associated Woronin bodies were similar among the taxa where they were observed. Sclerotia of Sclerotium cepivorum, which has no known teleomorph, are ultrastructurally most like sclerotia of Sclerotinia or Botrytis anamorphs of Botryotinia species. Substratal stromata of S. homoeocarpa showed unusually complex cellular organization. Sclerotial stromata of M. fructicola contained unusual storage vacuoles with heterogeneous contents.

Techniques for Transmission Electron Microscopy of Fiber-Matrix Interfaces in Aluminum Alloy-Boron Composites by Ion Erosio

1971 ◽  
Vol 29 ◽  
pp. 204-205
Author(s):  
G. G. Shaw
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Aluminum Alloy ◽  
Electron Beam ◽  
Pure Aluminum ◽  
Ion Milling ◽  
Transmission Electron ◽  
Fiber Matrix ◽  
Ion Erosion ◽  
Row Of Fibers

The morphology and composition of the fiber-matrix interface can best be studied by transmission electron microscopy and electron diffraction. For some composites satisfactory samples can be prepared by electropolishing. For others such as aluminum alloy-boron composites ion erosion is necessary.When one wishes to examine a specimen with the electron beam perpendicular to the fiber, preparation is as follows: A 1/8 in. disk is cut from the sample with a cylindrical tool by spark machining. Thin slices, 5 mils thick, containing one row of fibers, are then, spark-machined from the disk. After spark machining, the slice is carefully polished with diamond paste until the row of fibers is exposed on each side, as shown in Figure 1.In the case where examination is desired with the electron beam parallel to the fiber, preparation is as follows: Experimental composites are usually 50 mils or less in thickness so an auxiliary holder is necessary during ion milling and for easy transfer to the electron microscope. This holder is pure aluminum sheet, 3 mils thick.

Direct Observation of Heat Exchanger Deposits by Cross Sectioning

1967 ◽  
Vol 25 ◽  
pp. 364-365
Author(s):  
R. W. Anderson ◽  
D. L. Senecal
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Heat Exchanger ◽  
Direct Observation ◽  
Cross Sections ◽  
Preparation Method ◽  
Specimen Preparation ◽  
Flowing Water ◽  
Transmission Electron ◽  
Deposit Layer

A problem was presented to observe the packing densities of deposits of sub-micron corrosion product particles. The deposits were 5-100 mils thick and had formed on the inside surfaces of 3/8 inch diameter Zircaloy-2 heat exchanger tubes. The particles were iron oxides deposited from flowing water and consequently were only weakly bonded. Particular care was required during handling to preserve the original formations of the deposits. The specimen preparation method described below allowed direct observation of cross sections of the deposit layers by transmission electron microscopy.The specimens were short sections of the tubes (about 3 inches long) that were carefully cut from the systems. The insides of the tube sections were first coated with a thin layer of a fluid epoxy resin by dipping. This coating served to impregnate the deposit layer as well as to protect the layer if subsequent handling were required.

Phase Transformations in Ti-7w/oMo-3w/oMe Alloy

1974 ◽  
Vol 32 ◽  
pp. 514-515
Author(s):  
S. Fujishiro
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Transmission Electron Microscopy ◽  
Tensile Strength ◽  
Mechanical Processing ◽  
Ray Method ◽  
X Ray ◽  
Transmission Electron ◽  
Water Quench ◽  
Alpha Beta

The mechanical properties of three titanium alloys (Ti-7Mo-3Al, Ti-7Mo- 3Cu and Ti-7Mo-3Ta) were evaluated as function of: 1) Solutionizing in the beta field and aging, 2) Thermal Mechanical Processing in the beta field and aging, 3) Solutionizing in the alpha + beta field and aging. The samples were isothermally aged in the temperature range 300° to 700*C for 4 to 24 hours, followed by a water quench. Transmission electron microscopy and X-ray method were used to identify the phase formed. All three alloys solutionized at 1050°C (beta field) transformed to martensitic alpha (alpha prime) upon being water quenched. Despite this heavily strained alpha prime, which is characterized by microtwins the tensile strength of the as-quenched alloys is relatively low and the elongation is as high as 30%.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Comparative Microstructures of Ion Milled and Electrochemically Thinned Composite Materials

1974 ◽  
Vol 32 ◽  
pp. 546-547
Author(s):  
R.R. Russell
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Composite Materials ◽  
Great Difficulty ◽  
Ion Milling ◽  
Transmission Electron ◽  
Ion Damage ◽  
Intermetallic Composite

Transmission electron microscopy of metallic/intermetallic composite materials is most challenging since the microscopist typically has great difficulty preparing specimens with uniform electron thin areas in adjacent phases. The application of ion milling for thinning foils from such materials has been quite effective. Although composite specimens prepared by ion milling have yielded much microstructural information, this technique has some inherent drawbacks such as the possible generation of ion damage near sample surfaces.

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