SCREENING FOR HIGH-LYSINE CULTIVARS IN A BARLEY BREEDING PROGRAM

1976 ◽  
Vol 56 (4) ◽  
pp. 817-821 ◽  
Author(s):  
D. E. LABERGE ◽  
A. W. MACGREGOR ◽  
D. R. METCALFE
Keyword(s):  
Amino Acid ◽  
Normal Level ◽  
Amino Acid Analysis ◽  
Cereal Grains ◽  
High Lysine ◽  
Protein Levels ◽  
Hordeum Distichum ◽  
Barley Protein ◽  
Selection Of

The UDY dye-binding method for determining protein levels in cereal grains was applied to an F2 population derived from a cross between OR 585, a two-rowed selection of barley (Hordeum distichum L. emend Lam.) possessing the "Hiproly" gene(s) for high lysine and TR 412, a two-rowed selection with a normal level of lysine. Seed of the two parental gentoypes produce different UDY absorbance values at similar protein levels. If UDY absorbance values are determined for the parental genotypes over a range of barley protein contents, the different regression lines for OR 585 and TR 412 parental barleys can be used to segregate F2 progeny that express the gene for high lysine. Determination of lysine by the UDY method closely agrees with lysine values determined by amino acid analysis.

scholarly journals Amino acid analysis and N-terminal sequence determination of P7 proteolipid apoprotein from human myelin

FEBS Letters ◽  
1974 ◽  
Vol 45 (1-2) ◽  
pp. 295-298 ◽  
Author(s):  
J.L. Nussbaum ◽  
J.F. Rouayrenc ◽  
J. Jollès ◽  
P. Jollès ◽  
P. Mandel
Keyword(s):  
Amino Acid ◽  
Amino Acid Analysis ◽  
Terminal Sequence ◽  
Sequence Determination

Isolation, amino acid analysis and N-terminal sequence determination of the two subunits of the nickel-containing hydrogenase of Desulfovibrio gigas

Biochimie ◽  
1988 ◽  
Vol 70 (2) ◽  
pp. 267-271 ◽  
Author(s):  
Vincent Nivière ◽  
Nicole Forget ◽  
Gisèle Bovier-Lapierre ◽  
Jacques Bonicel ◽  
Claude Hatchikian
Keyword(s):  
Amino Acid ◽  
Amino Acid Analysis ◽  
Terminal Sequence ◽  
Sequence Determination ◽  
Desulfovibrio Gigas

scholarly journals 60 YEARS OF POMC: Lipotropin and beta-endorphin: a perspective

2016 ◽  
Vol 56 (4) ◽  
pp. T13-T25 ◽  
Author(s):  
D G Smyth
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Analysis ◽  
Structure And Function ◽  
Biologically Active ◽  
Molecular Endocrinology ◽  
And Function ◽  
The Brain ◽  
Quantitative Amino Acid Analysis

Many important fields of research had a humble origin. In the distant past, A J P Martin’s discovery that amino acids could be separated by paper chromatography and Moore and Stein’s use of columns for quantitative amino acid analysis provided the first steps towards the determination of structure in complex biologically active molecules. They opened the door to reveal the essential relationship that exists between structure and function. In molecular endocrinology, for example, striking advances have been made by chemists with their expertise in the identification of structure working with biologists who contributed valuable knowledge and experience. Advantage was gained from the convergence of different background, and it is notable that the whole is greater than the sum. In the determination of structure, it may be recalled that four of the world’s great pioneers (Archibald Martin, Rodney Porter, Fred Sanger and Vincent du Vigneaud) were acknowledged for their fundamental contributions when individually they were awarded the Nobel Prize. They foresaw that the identification of structure would prove of outstanding importance in the future. Indeed, study of the structures of β-endorphin and enkephalin and the different forms of opiate activity they engender has led to a transformation in our understanding of chemical transmission in the brain.

scholarly journals S6-RNase Is a Marker for Self-compatibility in Loquat (Eriobotrya japonica Lindl.)

HortScience ◽  
2010 ◽  
Vol 45 (8) ◽  
pp. 1146-1149 ◽  
Author(s):  
Reut Niska ◽  
Martin Goldway ◽  
Doron Schneider
Keyword(s):  
Amino Acid ◽  
System Analysis ◽  
The Self ◽  
Eriobotrya Japonica ◽  
Amino Acid Similarity ◽  
Self Fertilization ◽  
Self Compatibility ◽  
Fertilization System ◽  
Selection Of

Loquat (Eriobotrya japonica Lindl.), a member of the Rosaceae, carries the RNase-dependent gametophytic self-incompatibility fertilization system. Analysis of S-RNase-allele content in the commercial loquat cultivars Avri, Yehuda, and Akko 1 revealed that each of them contains one different S-RNase allele—S2, S3, and S4, respectively, and one that they all share, S6. Although all four S-alleles were isolated in this work, only S6 was found to be novel. Amino acid similarity between the partial sequence of S6-RNase and other known loquat RNases (S1 to S4) ranged between 62% and 65% with highest similarity (83%) to the S110-allele of European pear (Pyrus communis). Determination of S-RNase-allele content in progeny of ‘Avri’, ‘Yehuda’, and ‘Akko 1’, obtained in an open-pollinated, mixed-cultivar orchard, revealed that all of the progeny derived from self-fertilization contained the S6 haplotype, indicating that a mutation in the S6 locus is responsible for the self-fertilization. However, sequencing of most of the S6-RNase gene (from C1 to C5) did not reveal any mutation and the alignment of the deduced amino acid sequence showed that it has the expected S-RNase primary and tertiary structural organization. Nonetheless, because it is apparent that the S6-RNase allele is linked to the self-compatibility trait, it could serve as a marker for early selection of self-compatible loquat cultivars.

Determination of 2-Oxohistidine by Amino Acid Analysis

1995 ◽  
Vol 231 (2) ◽  
pp. 440-446 ◽  
Author(s):  
Sandra A. Lewisch ◽  
Rodney L. Levine
Keyword(s):  
Amino Acid ◽  
Amino Acid Analysis
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