ETHYL-METHANESULFONATE-INDUCED MUTATIONS IN RAPE (BRASSICA NAPUS)

1975 ◽  
Vol 55 (3) ◽  
pp. 817-821 ◽  
Author(s):  
D. B. FOWLER ◽  
B. R. STEFANSSON
Keyword(s):  
Brassica Napus ◽  
Flower Color ◽  
Ethyl Methanesulfonate ◽  
Induced Mutations ◽  
Brassica Napus L ◽  
Population Fitness

Ethyl-methanesulfonate (EMS)-treated rape (Brassica napus L.) populations were studied in the M2 generation. A large number and variety of visible deviates were observed and, of these, mutation frequencies of 28.3% for chlorophyll and 9.6% for flower color occurred in populations arising from a 1.0% EMS dose. A reduction in population fitness was related to increased EMS dose.

WHITE FLOWER COLOR IN RAPE (BRASSICA NAPUS) ASSOCIATED WITH A RADISH (RAPHANUS SATIVUS) CHROMOSOME

1982 ◽  
Vol 24 (6) ◽  
pp. 729-734 ◽  
Author(s):  
J. L. Sernyk ◽  
B. R. Stefansson
Keyword(s):  
Brassica Napus ◽  
Raphanus Sativus ◽  
Dominant Gene ◽  
Flower Color ◽  
Flowering Plants ◽  
White Flower ◽  
Genetic Constitution ◽  
Addition Line ◽  
Brassica Napus L ◽  
True Breeding

The inheritance of flower color was investigated in progeny from crosses between a normal, yellow flowering rape strain (Brassica napus L.) and a white flowering strain developed at the University of Manitoba by backcrossing the white flower color from Raphanobrassica into B. napus. The chromosome number of all yellow flowering plants examined was normal (2n = 38), whereas all white flowering plants examined contained a single or a pair of small radish (Raphanus sativus L.) chromosomes each carrying a dominant gene(s) for white flower color. The homozygous white flowering rape strain was in fact an alien addition line (2n = 40) disomic for a pair of radish chromosomes. The transmission and meiotic behavior of this alien chromosome as a univalent was investigated in the progeny of crosses involving the white flowering F1 hybrid. Cytological observations of meiotic metaphase 1 indicated that the univalent should be transmitted with 24.6% of the gametes. This was in agreement with the observed transmission of 24.3% through the female gametes. Transmission through the pollen ranged from 22.1% to 0.7%, the variation in certation depending on the genetic constitution of the pistil and on the time of pollination. The vigor, fertility and true breeding nature of the white flowering strain suggested that the white flower color factor from the radish had been introgressed into the rape genome. Cytological observations were necessary to demonstrate that introgression had not taken place and to aid in the interpretation of the genetic ratios observed.

EFFECTS OF THE MUTAGENIC AGENT ETHYL METHANESULFONATE ON THE M1 GENERATION OF RAPE (BRASSICA NAPUS)

1972 ◽  
Vol 52 (1) ◽  
pp. 53-62 ◽  
Author(s):  
D. B. FOWLER ◽  
B. R. STEFANSSON
Keyword(s):  
Brassica Napus ◽  
Seed Size ◽  
Seed Yield ◽  
Ethyl Methanesulfonate ◽  
Brassica Napus L ◽  
Plant Vigor ◽  
First Order ◽  
Mutagenic Agent ◽  
Days To Maturity

Germination of rape (Brassica napus L.) seed exposed to ethyl methanesulfonate (EMS) for 12 hr at 20–25 C was differentially affected by EMS concentration, pH of treatment solution, seed size, and strain treated. All first order interactions among treatments, except EMS concentration × strain, significantly affected germination. Increasing EMS concentration from 0 to 1.0% adversely affected germination, plant vigor, and seed yield and increased the number of abnormal plants and the days to maturity in M1 populations. The types of plant abnormalities observed following EMS treatment are described.

Reduction in sinapine content in rapeseed (Brassica napus L.) by induced mutations in sinapine biosynthesis genes

2015 ◽  
Vol 35 (1) ◽  
Author(s):  
Nazgol Emrani ◽  
Hans-Joachim Harloff ◽  
Olga Gudi ◽  
Friedrich Kopisch-Obuch ◽  
Christian Jung
Keyword(s):  
Brassica Napus ◽  
Induced Mutations ◽  
Brassica Napus L

scholarly journals Comparing the Resynthesis of Brassica napus L. by Interspecific Somatic and Sexual Hybridization. II. Hybrid Morphology and Identifying Organelle Genomes

1994 ◽  
Vol 119 (4) ◽  
pp. 816-823 ◽  
Author(s):  
Richard H. Ozminkowski ◽  
Pablo Jourdan
Keyword(s):  
Brassica Napus ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Flower Color ◽  
Brassica Napus L ◽  
Sexual Hybridization ◽  
Length Polymorphisms ◽  
Organelle Genomes ◽  
Mitochondrial Rflps ◽  
Restriction Fragment Length

Brassica napus (genome aacc), a natural allotetraploid derived from hybridization between B. oleracea L. (genome cc) and B. rapa L. (genome aa), was resynthesized by somatic and sexual hybridization. Seventy-two interspecific somatic (R0) hybrids and 27 sexual (F1) hybrids were produced from the same parent plants. R0 and F1 hybrids displayed morphology that was intermediate to the species parents, but B. rapa characteristics tended to predominate. R0 hybrids with nuclear DNA content equivalent to natural B. napus were uniform for nuclear-encoded traits, whereas allotetraploid F1 hybrids were variable for traits such as morphology, flower color, and seed production. Chloroplast restriction fragment length polymorphisms (RFLPs) showed unequal segregation in the R0 population favoring the chloroplasts of B. rapa; two of the 58 R0 hybrids tested had only the B. oleracea marker and 10 contained markers of both parents. Mitochondrial RFLPs showed a similar bias among the 56 R0 hybrids tested; only four plants showed B. oleracea markers exclusively, and the remaining plants were evenly distributed between having only B. rapa markers or having combinations from both species. In contrast, sexual hybrids displayed only maternal organelle markers.

Heterotic response and combining ability analysis in F1 diallel populations of Brassica napus L.

2019 ◽  
Vol 51 (6) ◽  
Author(s):  
Samrin Gul ◽  
Razi Uddin ◽  
Naqib Ullah Khan ◽  
Shahid Ullah Khan ◽  
Sardar Ali ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Combining Ability ◽  
Brassica Napus L ◽  
Combining Ability Analysis

Жирнокислотный состав масла семян селекционных линий ярового рапса (Brassica napus L.) в условиях Ленинградской области

2018 ◽  
pp. 12-17
Author(s):  
Т. В. Шеленга ◽  
А. В. Конарев ◽  
Л. П. Бекиш ◽  
Л. Ю. Новикова ◽  
И. Н. Перчук ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Brassica Napus L

Отмечена роль Н. И. Вавилова в организации исследований исходного и селекционного материала по биохимическим признакам, определяющим пищевые, кормовые и технологические качества сельскохозяйственной продукции. Выделен перспективный материал для создания сортов рапса с улучшенными пищевыми, кормовыми и технологическими характеристиками; выявлен характер зависимости признаков качества масла от погодных условий (температура, количество осадков и др.). Изучены 50 высокоэруковых (ВЭ) и 40 низкоэруковых (НЭ) линий ярового рапса (Brassica napus L.), выращенных в условиях Ленинградской области в 2012 – 2015 гг. Содержание масла и влажность в семенах определяли методом инфракрасной спектрометрии. Жирнокислотный состав (ЖКС) масла изучали с помощью газожидкостной хроматографии с масс-спектрометрией. Установлено содержание доминирующих жирных кислот для ВЭ и НЭ образцов, а также характер влияния погодных условий на ЖКС масла, в том числе на состав его отдельных фракций. Для ВЭ образцов установлена достоверная отрицательная корреляция (r = –0,96) между содержанием эруковой кислоты и температурой воздуха и достоверная положительная (r = 0,96) с количеством осадков в первую декаду июля. Для НЭ образцов установлена достоверная отрицательная зависимость между содержанием эруковой кислоты и средней температурой воздуха в мае. Выделенные образцы с высоким содержанием олеиновой (61,4 %) и низким линолевой (7,4 %) кислот можно рекомендовать для получения безэруковых сортов рапса, масло которых пригодно для длительного хранения. Контрастные по содержанию эруковой кислоты образцы будут использованы для создания сортов ярового рапса пищевого и кормового назначения, а также для производства биодизеля.

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