RELATIONSHIPS OF POPULATIONS OF TRICHODERMA SPP. IN SOIL TO DISEASE IN MAIZE
Several isolates of Trichoderma koningii Oud. aggr., T. harzianum Rifai aggr., and T. hamatum (Bon) Bain. aggr., recovered from maize (Zea mays L.) plants or maize field soil, produced first-internode lesions in maize seedlings. Lesions were produced in 70–100% of seedlings grown in soil containing 104 or 105 propagules of T. koningii per gram and in 0–10% of seedlings in soil with 103 propagules. The following selective medium was developed for quantitative recovery of Trichoderma spp. from soil: peptone–dextrose agar, rose bengal (17 mg/liter), streptomycin sulfate (30 mg/liter), and formalin (200 ppm). Numbers of propagules of Trichoderma spp. per gram of field soil cropped with maize under zero tillage or conventional tillage, or with soybeans (Glycine max L.) ranged from 5,300 to 15,600, 350 to 1,650, and 20 to 180, respectively, during the growing season. Populations of Trichoderma spp. in soil of maize microplots in which maize tissues were incorporated were greater than in those without added maize tissues. Emergence of maize seedlings grown in soil amended with maize tissues was 23–52% less than in soil without maize amendments, and many plants were stunted. The stunted plants shed pollen 7–10 days later, and produced 58% less grain than normal-sized plants.