OIL, FATTY ACID AND CHLOROPHYLL ACCUMULATION IN DEVELOPING SEEDS OF TWO "LINOLENIC ACID LINES" OF LOW ERUCIC ACID RAPESEED

1975 ◽  
Vol 55 (1) ◽  
pp. 197-203 ◽  
Author(s):  
G. RAKOW ◽  
D. I. McGREGOR
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Erucic Acid ◽  
Linolenic Acid ◽  
Acid Composition ◽  
Dry Weight ◽  
Brassica Napus L ◽  
Chlorophyll Contents ◽  
Developing Seeds ◽  
Low Erucic Acid

Fatty acid composition and oil formation were followed in developing seeds of two "linolenic acid lines" of low erucic acid rapeseed (Brassica napus L.) containing above and below normal linolenic acid levels to determine how these different levels of linolenic acid are derived. Under controlled growth room conditions, patterns of fresh weight and dry weight accumulation showed that seeds of both lines matured over the same period of time. During this time, both lines accumulated equivalent amounts of oil. The fatty acid composition of the oils of mature seed of both lines were similar for palmitic (16:0), palmitoleic (16:1), stearic (18:0), linoleic (18:2) and eicosenoic acid (20:1) when expressed either on a percent basis or a weight basis. However, oleic (18:1) and linolenic (18:3) acid were reciprocally related on a percent basis. The seeds of the two lines accumulated different weights of oleic and linolenic acid. Since the accumulation occurred over the same length of time, the difference in oleic and linolenic acid content was caused by different rates of accumulation. Equivalent chlorophyll contents, obtained for both lines, failed to show a relationship between chlorophyll content (indicative of photosynthetic activity) and linolenic acid accumulation.

GLC analysis of Indian rapeseed-mustard to study the variability of fatty acid composition

2000 ◽  
Vol 28 (6) ◽  
pp. 581-582 ◽  
Author(s):  
N. Kaushik ◽  
A. Agnihotri
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Erucic Acid ◽  
Linolenic Acid ◽  
Acid Composition ◽  
Acid Content ◽  
Erucic Acid Content ◽  
High Erucic Acid ◽  
Low Erucic Acid

Rapeseed-mustard is one of the most economically important oilseed crops in India. Speciality oils having high amounts of a specific fatty acid are of immense importance for both nutritional and industrial purposes. Oil high in oleic acid has demand in commercial food-service applications due to a long shelf-life and cholesterol-reducing properties. Both linoleic and linolenic acids are essential fatty acids; however, less than 3% linolenic acid is preferred for oil stability. High erucic acid content is beneficial for the polymer industry, whereas low erucic acid is recommended for food purposes. Therefore, it is important to undertake systematic characterization of the available gene pool for its variable fatty acid profile to be utilized for specific purposes. In the present study the Indian rapeseed-mustard germplasm and some newly developed low-erucic-acid strains were analysed by GLC to study the fatty acid composition in these lines. The GLC analysis revealed that the rapeseed-mustard varieties being commonly grown in India are characterized by high erucic acid content (30–51%) in the oil with low levels of oleic acid (13–23%). However, from among the recently developed low-erucic-acid strains, several lines were identified with comparatively high oleic acid (60–70%), moderate to high linoleic acid (13–40%) and low linolenic acid (< 10%) contents. Work is in progress at TERI (New Delhi, India) to utilize these lines for development of strains with particular fatty acid compositions for specific purposes.

LIPID AND MORPHOLOGICAL CHANGES IN DEVELOPING RAPESEED, BRASSICA NAPUS

1970 ◽  
Vol 50 (3) ◽  
pp. 233-247 ◽  
Author(s):  
D. B. FOWLER ◽  
R. K. DOWNEY
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Brassica Napus ◽  
Erucic Acid ◽  
Seed Development ◽  
Acid Composition ◽  
Oil Content ◽  
Morphological Changes ◽  
Dry Weight ◽  
Dry Matter Accumulation

Self-pollinated seed from normal and erucic acid free plants of summer rapeseed (Brassica napus L.) was harvested at weekly intervals from pollination to maturity. Oven-dried whole seeds and their component parts were weighed and analyzed for oil content and fatty acid composition. Oil and dry matter accumulation followed sigmoidal patterns, most of the deposition occurring between 14 and 35 days after pollination (DAP). The relative contribution of the testa, endosperm and embryo to dry weight and oil content of whole seeds changed significantly during seed development. Oil content of the developing embryo varied from 22 to 44%, and the testa from 1.6 to 13%, although at maturity only 6 to 8% oil was found in the testa and adhering aleurone. The nucleate endosperm oil content was estimated to be low and in the order of 2 to 2.5%. In 7- to 14-day-old seeds the dry weight, oil content and fatty acid composition were largely determined by the testa and endosperm. From 14 to 21 DAP the testa and embryo were dominant and after 21 DAP the embryo was the controlling influence on the seed characteristics studied.Oils of the testa, nucleate endosperm and embryo differed in fatty acid composition. In seeds free of erucic acid, the ratios of the 18 carbon fatty acids of the embryo and testa remained nearly constant from 21 DAP to maturity. This suggested that the variation in fatty acid composition as well as oil content during seed development in this material was due to disproportionate changes in the contribution of the testa, nucleate endosperm and embryo. However, in developing seeds capable of producing erucic acid a change in the ratio of fatty acid synthesis occurred in both the testa and embryo.

VARIETAL AND ENVIRONMENTAL EFFECTS ON RAPESEED: II. FATTY ACID COMPOSITION OF THE OIL

1959 ◽  
Vol 39 (4) ◽  
pp. 437-442 ◽  
Author(s):  
B. M. Craig ◽  
L. R. Wetter
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Liquid Phase ◽  
Erucic Acid ◽  
Acid Composition ◽  
Environmental Effects ◽  
Western Canada ◽  
Major Variation ◽  
Low Erucic Acid

The content of C16, C18, C20, C22 fatty acids were measured by gas liquid phase chromatography and linoleic and linolenic acids by spectral analyses on the oil from seven varieties of rapeseed grown at seven stations in Western Canada. Significant differences were found between varieties for all oil properties except the content of C16 acids. The major variation occurred in C18, C22 and linoleic acids with lesser amounts in the C20 and linolenic acids. The varieties Golden, Argentine, Regina II and Swedish are classed as high, Gute and Arlo as intermediate, and Polish as low erucic acid oils.

Chemical Constituents in Some Promising Genotypes of Indian Mustard [Brassica juncea (L.) Czern and Coss.]

2017 ◽  
Vol 4 (04) ◽  
Author(s):  
SUNITA SINGH ◽  
R. P. SINGH ◽  
H. K. SINGH ◽  
N. A. KHAN ◽  
M. K. MAURYA
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Erucic Acid ◽  
Brassica Juncea ◽  
Acid Composition ◽  
Chemical Constituents ◽  
Indian Mustard ◽  
Acid Value ◽  
Fatty Acid Analysis ◽  
Crude Fibre

Among the oilseed Brassica crops, Indian mustard [Brassica juncea (L.) Czern and Coss.] is an important source of oil from a nutritional point of view. The nutritional value of oil and cake quality is governed mainly by the composition of its fatty acids, iodine value, saponification, acid value, glucosinolates, crude fibre, protein and limiting amino acids, etc. Seventeen varieties/strains of Indian mustard were taken for saturated and unsaturated fatty acid analysis. The eicosenoic was absent in genotype (NUDBYJ-10) and erucic acid (NUDBYJ-10, LES-46 and Pusa mustard- 21). The fatty acid composition found a variable in different genotypes. Saturated fatty acid, Palmitic + Stearic ranged between 2.3 to 6.5%, Oleic 10.6 to 40.7%, Linoleic 16.1 to 37.7%, Linolenic 13.3 to 26.7%, Eicosenoic 0.00 to 10.30% and Erucic acid 0.00 to 47.50%, respectively. Alternaria blight severity also varied in different genotypes and ranged between 18.75 to 56.25%, maximum being in genotype Kranti and minimum in LES-47. No significant correlation was observed between the fatty acid composition and disease severity. The oil content range from 38.1 to 42.60% and protein content was found highest in variety RGN-73. The amino acid viz. methionine and tryptophan range between 0.41 to 1.81 g/16gN and 0.41 to 1.81 g /16g N, respectively.

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