VARIABILITY FOR IN VITRO DIGESTIBILITY OF CRESTED WHEATGRASS

1974 ◽  
Vol 54 (4) ◽  
pp. 651-657 ◽  
Author(s):  
B. E. COULMAN ◽  
R. P. KNOWLES
Keyword(s):  
Dry Matter ◽  
Bromus Inermis ◽  
Forage Yield ◽  
In Vitro Digestibility ◽  
In Vitro Testing ◽  
Narrow Sense ◽  
Sharp Decline ◽  
Crested Wheatgrass ◽  
Digestible Organic Matter

Significant differences in in vitro digestible organic matter (IVDOM) were found between plants of diploid Agropyron cristatum (L.) Gaertn. and tetraploid A. desertorum (Fisch.) Schult. Narrow-sense heritabilities for IVDOM ranged from 0.36 to 0.76. IVDOM showed no relationship to plant height, forage yield and lodging percentage, but was significantly correlated with leaf percentage. Diploid strains were more palatable than tetraploids and were significantly superior in IVDOM. A sharp decline in in vitro digestible dry matter (IVDDM) occurred from heading to the end of anthesis with little decline thereafter. Approximately 1 wk prior to anthesis, the IVDDM of crested wheatgrass was similar to that of bromegrass (Bromus inermis Leyss.), but at subsequent stages crested wheatgrass was inferior. It was concluded that limited progress in breeding for quality in crested wheatgrass was possible using in vitro testing.

scholarly journals Evaluation of Seven Oat (Avena sativa) Genotypes for Biomass Yield and Quality Parameters under Different Locations of Western Oromia, Ethiopia

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Abuye Tulu ◽  
Mekonnen Diribsa ◽  
Worku Temesgen
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Block Design ◽  
Quality Parameters ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Forage Crops ◽  
Yield And Quality ◽  
Digestible Organic Matter

Recognizing the potential and importance of cultivating improved forage crops as a means of tackling the recurrent feed shortage facing the study area, seven oat genotypes were tested in randomized complete block design with three replications across two locations for three growing seasons (2014, 2015, and 2016). The study was aimed to evaluate dry matter (DM) and digestible organic matter yield and nutrient composition of oat genotypes. The study revealed that oat genotypes responded differently for herbage dry matter (DM) and digestible organic matter (OM) yield, and quality parameters in both study locations. Averaged over the seven oat genotypes, herbage DM and digestible OM yield. recorded at Bako were higher than Boneya Boshe location across the study periods. The ash ( P  > 0.05) content did not vary among oat genotypes at both testing locations, while variation was observed for DM, crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), in vitro digestibility, and metabolizable energy (ME) constituents. In general, genotypes ILRI 6710 and 5453 showed higher herbage DM and digestible OM yield. Moreover, the two genotypes are also higher in their in vitro digestibility value and ME, DM, and CP contents but relatively lower in NDF, ADF, and ADL fiber constituents, and thus, they are recommended for wider cultivation.

CAUSAL EFFECTS AMONG FORAGE YIELD AND QUALITY MEASURES OI SMOOTH BROMEGRASS

1986 ◽  
Vol 66 (3) ◽  
pp. 591-600 ◽  
Author(s):  
M. D. CASLER
Keyword(s):  
Cell Wall ◽  
Path Analysis ◽  
Indirect Effects ◽  
Crude Protein ◽  
Dry Matter ◽  
Bromus Inermis ◽  
Forage Yield ◽  
Smooth Bromegrass ◽  
Dry Matter Digestibility

Future improvement of forage quality in smooth bromegrass (Bromus inermis Leyss.) and other species will require a knowledge of the chemical and structural factors most limiting to digestibility and intake of dry matter by ruminants. The objective of this study was to estimate direct and indirect effects from path analysis of several forage constituents on in vitro dry matter digestibility (IVDMD) and forage yield of smooth bromegrass. Six hundred and fifteen spaced plants of B8HD smooth bromegrass were harvested at Arlington, Wis. in June 1982 to assess dry matter yield and forage constituents. Near-infrared reflectance (NIR) spectroscopy was used to estimate IVDMD, crude protein, neutral detergent fiber, acid detergent fiber, cellulose, acid detergent hemicellulose, acid detergent lignin, and ash based on a random calibration subset of 75 plants. Five independent variables comprised the path analysis model: crude protein, cellulose, hemicellulose, lignin, and ash. None of the variables directly or indirectly affected forage yield. Lignin and cellulose concentrations had the overwhelmingly largest direct effects on IVDMD, while hemicellulose was important only when expressed as a percentage of the cell wall. Crude protein was important, but only to a small extent, while ash had no effect on IVDMD. The largest indirect effects on IVDMD were generally indirect effects of each character through correlated changes in lignin and indirect effects of lignin through correlated changes in other characters. The large effects of lignin support previous work establishing lignin as the primary factor limiting IVDMD. The importance of cellulose compared to hemicellulose, a result contrary to many current theories on the role of cell walls in rumen digestion, emphasizes at least three factors contributing to differences between this study and previous studies: (1) species differences, (2) genetic vs. species and maturity effects, and (3) analytical procedures. Current evidence suggests a cell wall ideotype should have reduced cellulose and lignin with either reduced or unchanged hemicellulose concentration.Key words: Bromus inermis Leyss., cellulose, hemicellulose, in vitro dry matter digestibility, lignin, forage yield

scholarly journals 127 Porcine in vitro digestibility of whole stillage predigested with multi-enzyme during different time periods

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 49-50
Author(s):  
Kevin S Jerez Bogota ◽  
Tofuko A Woyengo
Keyword(s):  
Dry Matter ◽  
In Vitro Digestion ◽  
Enzyme Treatment ◽  
Optimal Time ◽  
In Vitro Digestibility ◽  
Percentage Points ◽  
Whole Stillage ◽  
Freeze Dried ◽  
Different Sources

Abstract A study was conducted to determine the effects of the period of predigesting whole stillage (WS; slurry material that is dried into DDGS) with multi-enzyme and composition of the multi-enzyme on porcine in vitro digestibility of dry matter (IVDDM) of the WS. Four samples of whole stillage from 4 different sources were freeze-dried and divided into 13 subsamples to give 52 sub-samples. Thirteen treatments were applied to the 48 sub-samples within source. The treatments were undigested WS (control); or pre-digested with 1 of 3 multi-enzymes (MTE1, MTE2, and MTE3) at 55 °C for 6, 12, 18 or 24 h in 3 × 4 factorial arrangement. The MTE1 contained xylanase, β-glucanase, cellulase, mannanase, protease, and amylase; MTE2 contained xylanase, α-galactosidase, and cellulase; and MTE3 contained xylanase, cellulase, β-glucanase, and mannanase. The 52 subsamples were subjected to porcine in vitro digestion. The IVDDM of untreated WS was 73.3%. The IVDDM increased (P< 0.05) with an increase in the predigestion period. However, a rise in the predigestion period from 0 to 12 h resulted in greater (P< 0.05) response in mean IVDDM than an increment in the predigestion period from 12 to 24 h (11 vs. 0.83 percentage points). Predigestion period and multi-enzyme type interacted on IVDDM such that the improvement in IVDDM between 0 and 12 hours of predigestion differed (P< 0.05) among the 3 multi-enzyme types (13.3, 11.1, and 8.5 percentage points for MTE3, MTE2, and MTE1, respectively). The LS means by multi-enzyme treatment were modeled and resulted in unparallel curves (P< 0.05). The estimated maximum response of IVDDM for MTE1, MTE2 and MTE 3 were 82.4%, 84.7% and 87.1% at 15.8, 13 and 13.1 hours, respectively. In conclusion, the optimal time of predigestion of WS with multi-enzymes (with regard to improvement in its IVDDM) was approximately 14 h.

scholarly journals 49 Effects of in-vitro digestibility in cannulated steers when supplemented different levels of glycerol

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 55-56
Author(s):  
Noheli Gutierrez ◽  
Jamie A Boyd
Keyword(s):  
Dry Matter ◽  
Cold Water ◽  
Rumen Fluid ◽  
Latin Square ◽  
Optimal Level ◽  
Nutrient Digestibility ◽  
In Vitro Digestibility ◽  
Study Results ◽  
Different Levels

Abstract A study was conducted to evaluate effects of increasing concentration of food grade glycerol on rumen environment and nutrient digestibility. Three ruminally cannulated Jersey steers were used in this study. The study was conducted from March to May 2019. Experimental design was a 3x3 Latin square with a 2wk adjustment period followed by a 1wk collection period. Diet was coastal bermudagrass hay based. Different forage types were introduced in the incubation process to evaluate digestibility. Glycerol was administered once a day at 0, 15, or 20% of DMI (dry matter intake). dNDF (digestible NDF) and dDM (digestible dry matter) was determined using an ANKOM Daisy II incubator inoculated with 200g fresh rumen fluid and incubated for 12, 24, 48 and 72 h at 39°C. Each vessel contained ground forage samples in filter bags in triplicate. After incubation, filter bags were rinsed with cold water and dried for 24h in a 55°C forced air oven. Data were analyzed using the Proc MIXED procedure of SAS version 9.4. There was no difference dNDF in effect of different levels of glycerol between forage types by diet. But a numerical tendency was observed that dNDF was decreased at 20% inclusion rates in comparison to 0 and 15% inclusion of glycerol in the diet. Neither steer nor run was significantly different in the study. However as expected digestibility over time was significantly different (P < 0.001). A significant increase was observed in DMI with the increased levels of glycerol in the diet (P = 0.003), both the 15% and 20% levels of glycerol increased in DMI in comparison to the control (0%). It appears based on these study results that digestibility may be inhibited, as levels of dietary glycerol increase in the diet and more work needs to be done to find the optimal level of glycerol supplementation.

PREDICTION OF DIGESTIBLE ENERGY INTAKE BY SHEEP OF DIETS CONTAINING GROUND ROUGHAGE AND GRAIN

1977 ◽  
Vol 57 (2) ◽  
pp. 279-288 ◽  
Author(s):  
S. O. THORLACIUS
Keyword(s):  
Energy Demand ◽  
Dry Matter ◽  
Organic Matter Content ◽  
Rapeseed Meal ◽  
Regression Equation ◽  
Curvilinear Relationship ◽  
Crested Wheatgrass ◽  
Digestible Energy

Digestibility and intake of diets containing 8, 28, 48 or 68% ground wheat straw plus ground crested wheatgrass and rapeseed meal, and diets containing 33, 48, 63 and 78% ground crested wheatgrass plus barley and rapeseed meal was measured with four yearling wethers per diet. Digestible energy (DE) content ranged from 2.07 to 2.95 kcal/g dry matter (DM) and dry matter digestibility (DMD) (%) from 48.7 to 71.1%. Regression of DE intake y (kcal/w0.75kg/d) on DE content (x) was curvilinear; y = −2,133 + 1,626x − 277.9x2, r = 0.996, P < 0.01, SE = ± 7.3. There was also a curvilinear relationship between diet density, as fed, (x) g (DM)/ml and DMD (%), y = 9.057 + 364.1x − 530.0x2, r = 0.970, P < 0.01, SE = ± 2.4. A linear regression equation was calculated over the DE range (2.07–2.52) for which there was an obvious increase in DE intake with increasing diet DE content; y = −700.6 + 361x, r = 0.994, P < 0.01, SE = ± 9.4, y = DE intake (kcal/w0.75kg/d), x = DE [kcal/g (DM)]. Using this regression equation and assuming a linear increase in DE intake with increase in diet DE content up to a point at which the apparent energy demand of the animal is satisfied gave a more accurate prediction of DE intake than when the curvilinear regression equation, y = −2,133 + 1,626x − 277.9x2, was used empirically. Accuracy of the prediction was further improved by expressing DE/unit ration volume instead of per unit DM. The sheep used in the present experiments had an apparent energy demand of 230 kcal/w0.75kg/day which was met at diet DE contents above 0.48 kcal/ml or 2.6 kcal/g (DM). There was a high correlation between in vivo DE content of the diet, y [kcal/g (DM)] and in vitro (x) digestible organic matter content, x, (%), r = 0.991, P < 0.01, y = 0.38 + 0.037x, SE = ± 0.04.

The assessment of herbage legume varieties. II. In vitro digestibility, water soluble carbohydrate, crude protein and mineral content of primary growth of clover and lucerne

1966 ◽  
Vol 66 (3) ◽  
pp. 351-357 ◽  
Author(s):  
W. Ellis Davies ◽  
G. ap Griffith ◽  
A. Ellington
Keyword(s):  
White Clover ◽  
Crude Protein ◽  
Dry Matter ◽  
Red Clover ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Soluble Carbohydrate ◽  
In Vitro Digestibility ◽  
Primary Growth

The primary growth of eight varieties of three species–white clover (3), red clover (4) and lucerne (1)–was sampled at fortnightly intervals and the percentage dry matter, in vitro digestibility, crude protein, water soluble carbohydrates, P, Ca, K, Na and Mg were determined.Differences between species were nearly always significant and the general order of merit was white clover, red clover and lucerne. The exceptions were for dry-matter percentage where this order was reversed, and red clover had the lowest Na and highest Mg content.

scholarly journals COMPARISON OF NEAR INFRARED CALIBRATIONS FOR ESTIMATING IN VITRO DIGESTIBILITY IN WHOLE-PLANT CORN HYBRIDS

1987 ◽  
Vol 67 (2) ◽  
pp. 557-562 ◽  
Author(s):  
E. V. VALDES ◽  
R. B. HUNTER ◽  
G. E. JONES
Keyword(s):  
Dry Matter ◽  
Near Infrared ◽  
Mature Stage ◽  
In Vitro Digestibility ◽  
Infrared Reflectance ◽  
Near Infrared Reflectance ◽  
Corn Hybrids ◽  
Dry Matter Digestibility ◽  
Whole Plant

A comparison of two near infrared (NIRA) calibrations (C1 and C2) for the prediction of in vitro dry matter digestibility (IVDM) in whole-plant corn (WPC) was conducted. C1 consisted of 40 WPC samples collected from four locations across Ontario (Brucefield, London, Guelph and Elora). C2 consisted of 90 samples and included the above locations plus Pakenham and Winchester. Nine wavelengths were used in both equations but only three were common in C1 and C2 equations. These wavelengths were 2139 nm, 2100 nm, and 1445 nm, respectively. The predictions of IVDM utilizing both C1 and C2 were good. Coefficients of determination (r2) and standard error of the estimate (SEE) for calibration and prediction sets were 0.91, 1.7; 0.85, 1.7 for C1 and 0.88, 1.6; 0.77, 1.6 for C2 respectively. Regression analysis within location, however, showed low r2 values for the prediction of IVDM for Pakenham and Winchester in both calibrations. The more mature stage of harvest at these locations might be the cause of the poorer predictions. Key words: In vitro digestibility, whole-plant corn, near infrared reflectance

scholarly journals Nutritional value of tifton 85 bermudagrass at two cutting heights and different times of storage

2017 ◽  
Vol 38 (4) ◽  
pp. 2129
Author(s):  
Samantha Mariana Monteiro Sunahara ◽  
Marcela Abbado Neres ◽  
Jaqueline Rocha Wobeto Sarto ◽  
Caroline Daiane Nath ◽  
Kácia Carine Scheidt ◽  
...  
Keyword(s):  
Dry Matter ◽  
Nutritional Value ◽  
Ground Level ◽  
Matter Content ◽  
Fiber Content ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Dry Matter Content ◽  
Cutting Height

The goal of this study was to assess the dehydration curve and nutritional value of Tifton 85 bermudagrass at two cutting heights from ground level (4 and 8 cm) during 120 days of storage in a closed shed. The dehydration curve was determined using samples from the entire plant at eight different times. The experimental design consisted of randomized blocks with plots subdivided per times and five replicates. The second step consisted of assessing the nutritional value of the stored Tifton 85 bermudagrass in randomized blocks with plots subdivided per times and two treatments per plot: cutting height of four and eight centimeters from the ground, and five different times for the subplots, with five replicates. Dehydration of Tifton 85 bermudagrass at the two heights occurred in 48 hours, considered an ideal time for hay drying. The dry matter content responded quadratically to the time of storage of the two heights, only differing during baling and after 120 days of storage. Crude protein content had a quadratic behavior in the two cutting heights, with the smallest value after 30 days of storage (107.0 g kg-1) and the largest after 90 days (147.8 g kg-1) in the cutting height of eight centimeters. The ether extract exhibited a quadratic behavior in the two cutting heights, only differing after 90 days of storage. The neutral detergent fiber content had linear positive response according to the time of storage, with no difference between the cutting heights. For the neutral detergent fiber content in the two cutting heights, the quadratic regression model was the best fit to the data, differing between the heights after 30 and 60 days of storage. In vitro dry matter digestibility and in vitro cell wall digestibility values of the stored hay were lower than the values obtained at the time of cutting. Cutting performed at four centimeters from the ground was the most suitable for hay production due to higher dry matter production and nutritional value without difference between bailing treatments. Hay storage caused undesirable changes in the nutritional value, especially in fiber content and in vitro digestibility.

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