ALPHA AMYLASE DISTRIBUTION AND DDT RESPONSE IN CANADIAN BARLEY CULTIVARS

1971 ◽  
Vol 51 (5) ◽  
pp. 353-359 ◽  
Author(s):  
GEORGE FEDAK ◽  
TIBOR RAJHATHY
Keyword(s):  
Frequency Distribution ◽  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Foliar Spray ◽  
Alpha Amylase ◽  
Agar Gel ◽  
Barley Cultivars ◽  
Heat Stable ◽  
Agar Gel Electrophoresis

The heat-stable alpha amylase zymotype was studied by agar gel electrophoresis in 55 Canadian barley cultivars and most of their parents. A DC zymotype was found in 41 cultivars, DC1 in four, DC2C-DC polymorphism in nine and DC1-DC polymorphism in one. All cultivars were subjected to a foliar spray of DDT and all were found to be susceptible and monomorphic for this trait. These results are discussed in terms of cultivar identification, differences in frequency distribution of zymotypes between the European and Canadian material, and the introduction of these zymotypes into Canada.

POLYETHYLENE GLYCOL ON AGAR-GEL ELECTROPHORESIS

The Lancet ◽  
1974 ◽  
Vol 304 (7892) ◽  
pp. 1321-1322
Author(s):  
W.H. Taylor ◽  
D.J. Etherington
Keyword(s):  
Polyethylene Glycol ◽  
Gel Electrophoresis ◽  
Agar Gel ◽  
Agar Gel Electrophoresis

scholarly journals Hemoglobin Hope: A Beta Chain Variant

Blood ◽  
1965 ◽  
Vol 25 (5) ◽  
pp. 830-838 ◽  
Author(s):  
VIRGINIA MINNICH ◽  
ROBERT J. HILL ◽  
PHILIP D. KHURI ◽  
MARY E. ANDERSON
Keyword(s):  
Blood Cells ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Beta Chain ◽  
Agar Gel ◽  
Hemoglobin A ◽  
Abnormal Hemoglobin ◽  
Starch Gel ◽  
Agar Gel Electrophoresis ◽  
Chain Variant

Abstract A new hemoglobin, hemoglobin Hope, with a beta chain abnormality has been found in three generations of a St. Louis Negro family. The abnormal hemoglobin in the heterozygous state caused neither clinical stigmata nor abnormality in the red blood cells. Hemoglobin Hope was detected by agar gel electrophoresis at pH 6.2, but could not be differentiated from hemoglobin A by starch block electrophoresis at pH 8.6. Also, it could not be separated from hemoglobin A by paper, or starch gel electrophoresis employing a range of buffers from pH 6.2 to 8.6. Amino acid analysis showed that aspartic acid was substituted for glycine at position 136 of the beta chain. Hemoglobin Hope may be formulated as α2Aβ2136 gly-asp.

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