Populations, diversity and identities of bacterial endophytes in potato (Solanum tuberosum L.) cropping systems

2013 ◽  
Vol 93 (6) ◽  
pp. 1125-1142 ◽  
Author(s):  
Binod B. Pageni ◽  
Newton Z. Lupwayi ◽  
Francis J. Larney ◽  
Lawrence M. Kawchuk ◽  
Yantai Gan
Keyword(s):  
Solanum Tuberosum ◽  
Cropping Systems ◽  
Solanum Tuberosum L ◽  
Soil Management ◽  
Management Systems ◽  
Rrna Gene ◽  
Its Sequencing ◽  
Bacterial Endophytes ◽  
Dry Bean ◽  
Phylogenetic Characterization

Pageni, B. B., Lupwayi, N. Z., Larney, F. J., Kawchuk, L. M. and Gan, Y. 2013. Populations, diversity and identities of bacterial endophytes in potato ( Solanum tuberosum L.) cropping systems. Can. J. Plant Sci. 93: 1125–1142. Most plants host endophytic bacteria, but their identities and functions are usually unknown. Bacterial endophytes associated with potato grown after dry bean (Phaseolus vulgaris L.) or wheat (Triticum aestivum L.) were isolated, quantified and identified in a field study that compared crop rotations (3 to 6 yr in length) and soil management (CONV, conventional; CONS, conservation) for dry bean, potato, sugar beet (Beta vulgaris L.) and spring wheat. Populations of culturable endophytes ranged from 2.83×103 to 7.65×103 colony-forming units g−1 of root dry matter. The populations and diversity of the endophytes were greater with CONS than CONV soil management, and tended to be greater in longer than shorter rotations. The community structures of the endophytes were different between CONV and CONS soil management. A terminal-restriction fragment length polymorphism assay targeting the 16S rRNA gene, and its sequencing, showed that CONS management systems contained more Proteobacteria than CONV management systems, and vice-versa for Acidobacteria. Bacteriodetes were found only in long CONS rotations. This phylogenetic characterization of potato endophytes is important for further studies on their effects on the host plants.

Plant growth-promoting and phytopathogen-antagonistic properties of bacterial endophytes from potato (Solanum tuberosum L.) cropping systems

2014 ◽  
Vol 94 (5) ◽  
pp. 835-844 ◽  
Author(s):  
Binod B. Pageni ◽  
Newton Z. Lupwayi ◽  
Zafrin Akter ◽  
Francis J. Larney ◽  
Lawrence M. Kawchuk ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Cropping Systems ◽  
Solanum Tuberosum L ◽  
Soil Management ◽  
Shoot Biomass ◽  
Bacterial Endophytes ◽  
Hormone Production ◽  
Dry Bean ◽  
Plant Growth Promoting ◽  
Growth Promoting

Pageni, B. B., Lupwayi, N. Z., Akter, Z., Larney, F. J., Kawchuk, L. M. and Gan, Y. 2014. Plant growth-promoting and phytopathogen-antagonistic properties of bacterial endophytes from potato (Solanum tuberosum L.) cropping systems. Can. J. Plant Sci. 94: 835–844. Endophytes are microorganisms that live within a plant without harming it. Bacterial endophytes were isolated from roots of potatoes (Solanum tuberosum L.) grown under different rotations (3 to 6 yr in length) and soil management (CONV, conventional; CONS, conservation) in irrigated cropping systems with dry bean (Phaseolus vulgaris L.), sugar beet (Beta vulgaris L.) spring wheat (Triticum aestivum L.) and timothy (Phleum pratense L.). The endophytes were characterized for nitrogen fixation potential, phytohormone production and phytopathogen-antagonistic properties. The nitrogen-fixing nitrogenase (nifH) gene was detected in potato grown in all rotations, presumably partly because the soil in all rotations contained Rhizobium leguminosarum bv. phaseoli from the dry bean phase. Sequence analysis revealed that it was homologous to the genes found in Burkholderia, Azospirillum, Ideonella, Pseudacidovorax and Bradyrhizobium species. Indole acetic acid (IAA) hormone production by endophytes isolated from potato grown under CONS management was 66% greater than that those isolated from potato grown under CONV management, and tended to be greater in longer than shorter rotations. When 12 endophytes were inoculated to dry bean, four increased shoot biomass by 27–34%, and six increased total (shoot+root) biomass by 25% on average. Endophytes from the longer CONS rotations (4–6 yr) resulted in significantly higher (by 9%) shoot biomass than the shortest CONS (3 yr) rotation. Six of 108 endophyte isolates exhibited antagonistic properties (reduced pathogen biomass by 12 to 58% in dual culture assays in liquid media) against potato pathogens Pectobacterium atrosepticum, Fusarium sambucinum and Clavibacter michiganensis subsp. epedonicus. All the six isolates were from CONS soil management. Therefore, the benefits of long rotations, with their associated CONS soil management, to crop productivity in these irrigated cropping systems probably include nutritional (biological nitrogen fixation and IAA hormone production) and disease-control benefits imparted by endophytic bacteria.

Soil micro-arthropod communities and microbial parameters in the potato ridge under two field management systems on sandy loams in Atlantic Canada

2007 ◽  
Vol 87 (4) ◽  
pp. 399-404 ◽  
Author(s):  
M R Carter ◽  
C. Noronha
Keyword(s):  
Microbial Biomass ◽  
Integrated Pest Management ◽  
Pest Management ◽  
Microbial Biomass Carbon ◽  
Solanum Tuberosum L ◽  
Soil Management ◽  
Management Systems ◽  
Microbial Biomass C ◽  
Atlantic Canada ◽  
Soil Microbial

Intensive forms of soil management occur in potato (Solanum tuberosum L.) production systems, but little is known about the influence of such practices on soil biological properties. Microbial biomass C, phosphatase activity, and the abundance (number), richness (family groups), and diversity of soil micro-arthropods (Collembola and mites) were compared in conventional and adjacent integrated pest management (IPM) systems of 3-yr potato rotations, established on fine sandy loams in Prince Edward Island, Atlantic Canada. The study was conducted at two sites over a 2-yr period. Soil microbial parameters were generally similar between management systems. Management differences showed some effect on micro-arthropod abundance and richness in three of the eight comparisons. Under optimum soil-water conditions, both Collembola and mite communities increased over the growing season regardless of management system. Key words: Soil management for potato, Collembola, mites, soil microbial biomass carbon, acid phosphatase, integrated pest management

Residual effects of ethametsulfuron on succeeding broadleaf crops

1995 ◽  
Vol 75 (4) ◽  
pp. 941-943
Author(s):  
D. A. Wall
Keyword(s):  
Solanum Tuberosum ◽  
Key Words ◽  
Helianthus Annuus ◽  
Solanum Tuberosum L ◽  
Residual Effects ◽  
Navy Bean ◽  
Dry Bean ◽  
Crop Tolerance ◽  
Potato Yields ◽  
Fagopyrum Esculentum Moench

The response of dry bean (Phoseolus vulgaris L.), buckwheat (Fagopyrum esculentum Moench.), potato (Solanum tuberosum L.) and sunflower (Helianthus annuus L.) to soil residues of ethametsulfuron was determined. Buckwheat and sunflower exhibited moderate to severe injury 4 wk after planting; navy bean, buckwheat, sunflower and potato yields were unaffected in the cropping year after ethametsulfuron application. Key words: Sulfonylurea, crop tolerance, yield

scholarly journals CHARACTERIZATION OF 18S RIBOSOMAL RNA FRAGMENT FROM Solanum tuberosum L. var. Granola POTATO

2018 ◽  
Vol 5 (1) ◽  
pp. 27
Author(s):  
Yunior William Susanto ◽  
Hermin Pancasakti Kusumaningrum ◽  
Siti Nur Jannah ◽  
Sri Rustini
Keyword(s):  
Solanum Tuberosum ◽  
Ribosomal Rna ◽  
Solanum Tuberosum L ◽  
Phylogenetic Reconstruction ◽  
Reference Sequence ◽  
Rrna Gene ◽  
Gene Fragment ◽  
Potato Leaf ◽  
Pcr Method

Potato (Solanum tuberosum L.) is a prime horticultural commodity. One of the varieties of potato that widely cultivated in Indonesia is Granola. This study characterized the variety Granola based on the 18S sequences of rRNA gene fragment. The 18S sequences were used to distinguish the Granola and determine the differentiating characters from other Solanum based on those sequences data. The characterization was completed in three main steps including DNA isolation from potato leaf using Doyle & Doyle method, amplification of the 18S gene fragment, and DNA sequencing. The amplification of 18S gene fragment by a PCR method obtained 528 bp sequences. The BLAST search using NCBI web service confirmed that Granola potato has 99% matching sequence with S. tuberosum. The phylogenetic reconstruction further indicates the S. tuberosum var. Granola used in this study deeply nested with the reference sequence X67238.1, a potato from Europe.

scholarly journals SOIL FUNGISTASIS AGAINST FUSARIUM GRAMINEARUM UNDER DIFFERENT CROP MANAGEMENT SYSTEMS

2015 ◽  
Vol 39 (1) ◽  
pp. 69-77 ◽  
Author(s):  
Bruno Brito Lisboa ◽  
Cimélio Bayer ◽  
Luciane Maria Pereira Passaglia ◽  
Flávio Anastácio de Oliveira Camargo ◽  
Anelise Beneduzi ◽  
...  
Keyword(s):  
Fusarium Graminearum ◽  
Cropping Systems ◽  
Soil Management ◽  
Biological Properties ◽  
Plant Diseases ◽  
Management Systems ◽  
Genetic Profile ◽  
No Tillage ◽  
Bacterial Isolates ◽  
Soil Fungistasis

Soil management, in terms of tillage and cropping systems, strongly influences the biological properties of soil involved in the suppression of plant diseases. Fungistasis mediated by soil microbiota is an important component of disease-suppressive soils. We evaluated the influence of different management systems on fungistasis against Fusarium graminearum, the relationship of fungistasis to the bacterial profile of the soil, and the possible mechanisms involved in this process. Samples were taken from a long-term experiment set up in a Paleudult soil under conventional tillage or no-tillage management and three cropping systems: black oat (Avena strigose L.) + vetch (Vicia sativa L.)/maize (Zea mays L.) + cowpea (Vigna sinensis L.), black oat/maize, and vetch/maize. Soil fungistasis was evaluated in terms of reduction of radial growth of F. graminearum, and bacterial diversity was assessed using ribosomal intergenic spacer analysis (RISA). A total of 120 bacterial isolates were obtained and evaluated for antibiosis, and production of volatile compounds and siderophores. No-tillage soil samples showed the highest level of F. graminearum fungistasis by sharply reducing the development of this pathogen. Of the cropping systems tested, the vetch + black oat/maize + cowpea system showed the highest fungistasis and the oat/maize system showed the lowest. The management system also affected the genetic profile of the bacteria isolated, with the systems from fungistatic soils showing greater similarity. Although there was no clear relationship between soil management and the characteristics of the bacterial isolates, we may conclude that antibiosis and the production of siderophores were the main mechanisms accounting for fungistasis.

Situación económica y social de la población de San José del Carmen, San Cristóbal de las Casas, Chiapas

2019 ◽  
Vol 12 (4) ◽  
Author(s):  
Pedro Cadena-Iñiguez ◽  
Eileen Salinas-Cruz ◽  
Jesús Martínez-Sánchez ◽  
Mariano Morales-Guerra ◽  
Romualdo Vásquez-Ortiz ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Theobroma Cacao ◽  
Zea Mays L ◽  
Solanum Tuberosum L ◽  
San Jose ◽  
Phaseolus Vulgaris L ◽  
Las Casas ◽  
Vicia Faba L ◽  
Las Mujeres ◽  
Theobroma Cacao L

Objetivo: Establecer una línea base como fundamento para la intervención e inducción de innovaciones a través de escuelas de campo y planes de negocios. Diseño/metodología/aproximación: El estudio se desarrolló en San José del Carmen, San Cristóbal de Las Casas, Chiapas, México. Se elaboró una encuesta como instrumento para recabar en campo información de familias de la localidad con la finalidad de conocer su situación actual. La localidad de trabajo se eligió utilizando como criterio las poblaciones que se encuentran dentro de Cruzada Nacional contra el hambre y en la clasificación de la pobreza extrema Resultados: La localidad de San José del Carmen es considerada como de alta marginación, la población es bilingüe, su principal lengua es el Tzotzil, el nivel de estudios promedio es hasta el tercer año de primaria, las principales actividades productivas son la siembra de maíz (Zea mays L.), frijol (Phaseolus vulgaris L.), papa (Solanum tuberosum L.) y haba (Vicia faba L.) que son utilizadas principalmente para autoconsumo.  No cuentan con agua potable ya que su sistema es a través de la recaudación de agua de lluvia o a través de un jagüey. Sus principales actividades económicas son la albañilería por parte de los hombres, y venta de productos, tales como el pozol (bebida energética a base de maíz y cacao (Theobroma cacao L.), tostadas y algunos vegetales por parte de las mujeres. Las tostadas son producidas en forma artesanal con un proceso de doble nixtamalización y vendida en bolsas de 20 tostadas cada una, a un costo de MX$10.00 en los mercados de San Cristóbal de las Casas, una a dos veces por semana alrededor de 40 bolsas por día de venta. Limitaciones del estudio/implicaciones: La afiliación zapatista de alguno de los pobladores y el hecho de que algunos habitantes no hablen español, fueron factores que limitaron desarrollar al 100% el estudio; sin embargo, se considera que se tienen los elementos necesarios para establecer la línea base de la situación de San José del Carmen. Conclusiones: El diagnóstico servirá como una herramienta para establecer una intervención a través de innovaciones y planes de negocios en la comunidad de estudio. Los pobladores cuentan con herramientas necesarias para obtener nuevos conocimientos que ayuden a un desarrollo de su producción. El idioma no deberá de ser una limitante para ello.

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