The damage repair role of He-Ne laser on wheat exposed to osmotic stress

2010 ◽  
Vol 90 (5) ◽  
pp. 691-698 ◽  
Author(s):  
Z. -B. Qiu ◽  
J. -T. Li ◽  
M. Yue
Keyword(s):  
Triticum Aestivum ◽  
Laser Radiation ◽  
Osmotic Stress ◽  
Production Rate ◽  
Antioxidative Enzymes ◽  
Triticum Aestivum L ◽  
Wheat Seedlings ◽  
Damage Repair ◽  
Helium Neon

In order to determine the damage repair role of helium-neon (He-Ne) laser on wheat (Triticum aestivum L.) exposed to osmotic stress, 12-d-old seedlings (with two fully expanded leaves) were treated with osmotic stress using 5% (wt/vol), 10% (wt/vol) and 15% (wt/vol) polyethylene glycol (PEG 6000) treatment for 9 d. After 9 d of osmotic stress, a He-Ne laser was employed to irradiate seedlings of spring wheat for 0 min, 1 min and 3 min. Changes in the concentration of malondialdehyde (MDA), hydrogen peroxide (H2O2), glutathione (GSH), ascorbate (AsA), the production rate of superoxide radical (O2), the activities of ascorbate peroxidase (APX), peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) were measured to test the effects of laser radiation. The results showed that laser radiation for 3 min conferred tolerance to osmotic stress in wheat seedlings by decreasing the concentration of MDA and the production rate of O2, and increasing the activities of SOD and APX and GSH concentration. It was suggested that those changes in MDA, O2, antioxidative enzymes and antioxidative compounds were responsible for the increase in osmotic stress tolerance observed in the experiments. Therefore, antioxidative enzymes and antioxidative compounds may participate in the repair effect of laser on seedlings under osmotic stress. This is the first investigation reporting the damage repair role of He-Ne laser on plants exposed to osmotic stress.Key words: Helium-neon laser, wheat (Triticum aestivum L.), osmotic stress, antioxidative system

scholarly journals Response of growth and antioxidant enzymes to osmotic stress in two different wheat (Triticum aestivum L.) cultivars seedlings

2012 ◽  
Vol 58 (No. 12) ◽  
pp. 534-539 ◽  
Author(s):  
G.Q. Wu ◽  
L.N. Zhang ◽  
Y.Y. Wang
Keyword(s):  
Triticum Aestivum ◽  
Antioxidant Enzymes ◽  
Osmotic Stress ◽  
Wheat Cultivar ◽  
Triticum Aestivum L ◽  
Shoot Length ◽  
The Other ◽  
Wheat Cultivars ◽  
Wheat Seedlings ◽  
Drought Tolerant

 To investigate the responses of growth and antioxidant enzymes to osmotic stress in two different wheat cultivars, one drought tolerant (Heshangtou, HST) and the other drought sensitive (Longchun 15, LC15), 15-day-old wheat seedlings were exposed to osmotic stress of –0.25, –0.50, and –0.75 MPa for 2 days. It is found that osmotic stress decreased shoot length in both wheat cultivars, whereas to a lesser degree in HST than in LC15. The contents of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) of shoot in both wheat cultivars were increased by osmotic stress. It is clear that MDA contents increased less in the more drought tolerant cultivar HST than in drought sensitive one LC15. On the contrary, POD and CAT activities increased more in HST than LC15 under osmotic stress. As the activity of SOD, however, no significant differences were found between HST and LC15. These results suggest that wheat cultivar HST has higher activities of antioxidant enzymes such as POD and CAT to cope with oxidative damage caused by osmotic stress compared to sensitive LC15.  

Effect of 24-epibrassinolide on localization of ABA, AZP, and dehydrins in wheat plant roots during dehydration

Biomics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 329-336
Author(s):  
A.R. Lubyanova ◽  
F.M. Shakirova ◽  
M.V. Bezrukova
Keyword(s):  
Triticum Aestivum ◽  
Wheat Germ ◽  
Wheat Plant ◽  
Triticum Aestivum L ◽  
Immunohistochemical Localization ◽  
Plant Roots ◽  
Wheat Roots ◽  
Wheat Seedlings ◽  
Apoplastic Barrier ◽  
Protective Proteins

We studied the immunohistochemical localization of abscisic acid (ABA), wheat germ agglutinin (WGA) and dehydrins in the roots of wheat seedlings (Triticum aestivum L.) during 24-epibrassinolide-pretreatment (EB-pretreatment) and PEG-induced dehydration. It was found coimmunolocalization of ABA, WGA and dehydrins in the cells of central cylinder of basal part untreated and EB-pretreated roots of wheat seedlings under normal conditions and under osmotic stress. Such mutual localization ABA and protective proteins, WGA and dehydrins, indicates the possible effect of their distribution in the tissues of EB-pretreated wheat roots during dehydration on the apoplastic barrier functioning, which apparently contributes to decrease the water loss under dehydration. Perhaps, the significant localization of ABA and wheat lectin in the metaxylem region enhances EB-induced transport of ABA and WGA from roots to shoots under stress. It can be assumed that brassinosteroids can serve as intermediates in the realization of the protective effect of WGA and wheat dehydrins during water deficit.

scholarly journals Enhancement of drought tolerance in Triticum aestivum L. seedlings using Azospirillum brasilense NO40 and Stenotrophomonas maltophilia B11

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Wedad A. Kasim ◽  
Mohamed E. H. Osman ◽  
Mohamed N. Omar ◽  
Samar Salama
Keyword(s):  
Triticum Aestivum ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Azospirillum Brasilense ◽  
Stenotrophomonas Maltophilia ◽  
Protein Pattern ◽  
Triticum Aestivum L ◽  
Protein Patterns ◽  
Wheat Seedlings ◽  
Enhanced Production

Abstract Background The effectiveness of two PGPB; Azospirillum brasilense NO40 and Stenotrophomonas maltophilia B11 was investigated in enhancing the drought tolerance of wheat (Triticum aestivum L.) seedlings cultivar Gemiza9. The inoculated or uninoculated grains were sown in unsterilized sandy soil and watered normally untill the 8th day. Drought stress was initiated by completely withholding water for 7 days (until wilting). Samples were collected after 15 days from sowing to evaluate some growth criteria, damage and defense indicators and to analyze the roots’ protein pattern. Results The results showed that inoculating wheat seedlings with these strains significantly diminished the inhibitory effects of drought stress on the relative water content of roots, shoots and leaves; area of leaves; contents of pigments (chlorophyll a and b) and ascorbic acid; and on the protein patterns of roots. Moreover, the bacterial inoculation notably reduced the drought-induced damage indicated by lower leakage of electrolytes and less accumulation of Malondialdehyde and hydrogen peroxide, surprisingly with less enhanced production of proline and activities of catalase and peroxidase than their uninoculated counterparts. Under normal conditions, inoculating wheat plants with these PGPB resulted in significantly promoted growth and elevated contents of pigments and altered protein patterns of roots. Conclusion Overall, we can say that both Azospirillum brasilense NO40 and Stenotrophomonas maltophilia B11 were able to deactivate the growth inhibition in wheat seedlings to some extent, while maintaining a certain level of efficient protection against damage under drought stress.

scholarly journals Pivotal role of bZIPs in amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Pankaj Kumar ◽  
Ankita Mishra ◽  
Himanshu Sharma ◽  
Dixit Sharma ◽  
Mohammed Saba Rahim ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Transcriptome Analysis ◽  
Triticum Aestivum L ◽  
Pivotal Role ◽  
Genome Survey

The Role of Leaf Epicuticular Wax in the Adaptation of Wheat ( Triticum aestivum L.) to High Temperatures and Moisture Deficit Conditions

Crop Science ◽  
2018 ◽  
Vol 58 (2) ◽  
pp. 679-689 ◽  
Author(s):  
Suheb Mohammed ◽  
Trevis D. Huggins ◽  
Francis Beecher ◽  
Chris Chick ◽  
Padma Sengodon ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
High Temperatures ◽  
Triticum Aestivum L ◽  
Epicuticular Wax ◽  
Moisture Deficit ◽  
Leaf Epicuticular Wax
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