scholarly journals Empirical verification of heterogeneous DNA fragments generated from wheat genome-specific SSR primers

2008 ◽  
Vol 88 (6) ◽  
pp. 1065-1071 ◽  
Author(s):  
Qijiao Chen ◽  
Lianquan Zhang ◽  
Zhongwei Yuan ◽  
Zehong Yan ◽  
Youliang Zheng ◽  
...  
Keyword(s):  
Common Wheat ◽  
Hexaploid Wheat ◽  
Chinese Spring ◽  
Triticum Turgidum ◽  
Sequence Data ◽  
Aegilops Tauschii ◽  
Single Locus ◽  
Synthetic Hexaploid Wheat ◽  
D Genome ◽  
Synthetic Hexaploid

Due to the high polymorphisms between synthetic hexaploid wheat (SHW) and common wheat, SHW has been widely used in genetic studies. The transferability of simple sequence repeats (SSR) among common wheat and its donor species, Triticum turgidum and Aegilops tauschii, and their SHW suggested the possibility that some SSRs, specific for a single locus in common wheat, might appear in two or more loci in SHWs. This is an important genetic issue when using synthetic hexaploid wheat population and SSR for mapping. However, it is largely ignored and never empirically well verified. The present study addressed this issue by using the well-studied SSR marker Xgwm261 as an example. The Xgwm261 produced a 192 bp fragment specific to chromosome 2D in common wheat Chinese Spring, but generated a 176 bp fragment in the D genome of Ae. tauschii AS60. Chromosomal location and DNA sequence data revealed that the176 bp fragment also donated by 2B chromosome of durum wheat Langdon. These results indicated that although a single 176 bp fragment was appeared in synthetic hexaploid wheat Syn-SAU-5 between Langdon and AS60, the fragment contained two different loci, one from chromosome 2D of AS60 and the other from 2B of Langdon which were confirmed by the segregating analysis of SSR Xgwm261 in 185 plants from a F2 population between Syn-SAU-5 and Chinese Spring. If Xgwm261 in Syn-SAU-5 was considered as a single locus in genetic analysis, distorted segregation or incorrect conclusions would be yielded. A proposed strategy to avoid this problem is to include SHW’s parental T. turgidum and Ae. tauschii in SSR analysis as control for polymorphism detection. Key words: Synthetic hexaploid wheat, microsatellite, segregation distortion, Xgwm261, transferability

scholarly journals Allopolyploidization Enhances Genetic Recombination of the Ancestral Diploid Genome in the Evolution of Wheat

2020 ◽  
Author(s):  
Hongshen Wan ◽  
Jun Li ◽  
Shengwei Ma ◽  
Fan Yang ◽  
Liang Chai ◽  
...  
Keyword(s):  
Hexaploid Wheat ◽  
Genetic Recombination ◽  
Recombination Frequency ◽  
Aegilops Tauschii ◽  
Synthetic Hexaploid Wheat ◽  
Evolutionary Potential ◽  
D Genome ◽  
Diploid Genome ◽  
Synthetic Hexaploid ◽  
The Impact

Abstract Background: Genetic recombination produces different allelic combinations potentially, providing new variations to the selection pools for domestication. Allopolyploidization increases evolutionary potential of the hexaploid common wheat by taking its advantages of heterosis and gene redundancy. May there be any relationship between allopolyploidization and genetic recombination? To study the impact of allopolyploidization on genetic recombination in different ancestral genomes of wheat, we generated synthetic hexaploid wheat by crossed tetraploid Triticum turgidum with diploid Aegilops tauschii to simulate its evolutionary hexaploidization process. Results: Using Wheat Breeder’s Genotyping Array, the genotypes of F2 individuals were investigated in both tetraploid (A1A1B1B1 x A2A2B2B2) and their synthetic hexaploid wheat derived populations (A1A1B1B1DD x A2A2B2B2DD). And the genotypes of the diploid population (D1D1 x D2D2) and their synthetic hexaploid wheat derived population (AABBD1D1 x AABBD2D2) were obtained with DArT-Seq™ technology. Based on genotypes of F2 populations, the genetic recombination frequency of homologous chromosome were consequently calculated in ancestral tetraploid AABB (4x), diploid DD (2x) and their synthetic hexaploid AABBDD (6x) plants, respectively. The recombination frequency of the ancestral diploid genome DD from Aegilops tauschii was found enhanced significantly more than 2 folds after their hexaploidization, while no significant changes was found in their ancestral tetraploid genome AABB via hexaploidization.Conclusions: Allopolyploidization enhancing genetic recombination of the ancestral diploid genome is found to increase the evolutionary potential of wheat, which is beneficial for wheat to conquer their narrow origination of D genome, quickly spread and make it a major crop of the world.

scholarly journals Development and Identification of Four New Synthetic Hexaploid Wheat Lines with Solid Stems

2021 ◽  
Author(s):  
Dongyu Liang ◽  
Minghu Zhang ◽  
Xin Liu ◽  
Hui Li ◽  
Zhenjiao Jia ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Hexaploid Wheat ◽  
Triticum Turgidum ◽  
Chromosome Doubling ◽  
Aegilops Tauschii ◽  
Synthetic Hexaploid Wheat ◽  
Vascular Bundles ◽  
Lodging Resistance ◽  
Synthetic Hexaploid ◽  
Wheat Lines

Abstract Stem solidness is an important agronomic trait for increasing the ability of wheat to resist lodging. In this study, four new synthetic hexaploid wheat with solid stems were developed from natural chromosome doubling of F1 hybrids between a solid-stemmed durum wheat (Triticum turgidum ssp. durum, 2n = 4x = 28, AABB) and four Aegilops tauschii (2n = 2x = 14, DD) accessions. The solid expression of the second internode at the base of the stem was stable for two synthetic hexalpoid wheat Syn-SAU-117 and Syn-SAU-119 grown in both the greenhouse and field. The lodging resistance of four synthetic solid-stem wheats is stronger than that of CS, and Syn-SAU-116 has the strongest lodging resistance, followed by Syn-SAU-119. The paraffin sections of the second internode showed that four synthetic wheat lines had large outer diameters, well-developed mechanical tissues, large number of vascular bundles, and similar anatomical characteristics with solid-stemmed durum wheat. The chromosomal composition of four synthetic hexaploid wheat was identified by FISH (fluorescence in situ hybridization) using Oligo-pSc119.2-1 and Oligo-pTa535-1. At adult stage, all four synthetic hexaploid wheat showed high resistance to mixed physiological races of stripe rust pathogen (CYR31, CYR32, CYR33, CYR34). These synthetic hexaploid wheat lines provide new materials for the improvement of common wheat.

scholarly journals Development and Characterization of Synthetic Hexaploid Wheat for Improving the Resistance of Common Wheat to Leaf Rust and Heat Stress

Agronomy ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Hai An Truong ◽  
Hyeri Lee ◽  
Masahiro Kishii ◽  
Suk Whan Hong ◽  
Hojoung Lee
Keyword(s):  
Heat Stress ◽  
Leaf Rust ◽  
Common Wheat ◽  
Hexaploid Wheat ◽  
Aegilops Tauschii ◽  
Synthetic Hexaploid Wheat ◽  
Synthetic Hexaploid ◽  
Shock Proteins ◽  
Wheat Lines

Synthetic hexaploid wheat (SHW) is a valuable resource for breeding because it possesses more desirable traits, such as better yield and abiotic and biotic stress tolerance than common wheat. In this study, our group developed a SHW line, named ‘SynDT’, which has markedly better characteristics than Korean bread wheat ‘Keumkang’. The SynDT line is thermotolerant as it rapidly expresses heat shock proteins under heat stress. In addition, this line exhibits resistance to leaf rust by inducing the expression of antifungal enzymes, mainly chitinase, along with the rapid and high expression of pathogen-related genes. Moreover, it possesses the favorable traits of its parent wheat lines Triticum durum #24 and Aegilops tauschii #52. Therefore, the SynDT wheat line can be used as a breeding material for improving local common wheat cultivars.

scholarly journals Polyploidization enhancing genetic recombination of the ancestral diploid genome in the evolution of hexaploid wheat

2020 ◽  
Author(s):  
Hongshen Wan ◽  
Jun Li ◽  
Shengwei Ma ◽  
Qin Wang ◽  
Xinguo Zhu ◽  
...  
Keyword(s):  
Hexaploid Wheat ◽  
Genetic Recombination ◽  
Aegilops Tauschii ◽  
Tetraploid Wheat ◽  
Synthetic Hexaploid Wheat ◽  
Evolutionary Potential ◽  
D Genome ◽  
Diploid Genome ◽  
Synthetic Hexaploid ◽  
The Impact

AbstractAllopolyploidy increases its evolutionary potential by fixing heterosis and the advantage of gene redundancy. Allelic combinations generated from genetic recombination potentially provide many variations to the selection pools for evolution. May there be any relationship between allopolyploidization and genetic recombination? To study the impact of polyploidy on genetic recombination, we selected wheat as a model and simulated its evolution pathway of allopolyploidy by developing synthetic hexaploid wheat. The change of homologous chromosome recombination were investigated on their diploid DD and tetraploid AABB genomes after their allohexaploidization, respectively. The genetic recombination of the ancestral diploid genome of Aegilops tauschii was enhanced significantly more than 2 folds after their hexaploidization. Hexaploidization enhancing genetic recombination of the ancestral diploid D genome was firstly reported to be a new way to increase evolutionary potential of wheat, which is beneficial for wheat to conquer their narrow origination of D genome, quickly spread and make it a major crop of the world. Finally, re-synthetizing hexaploid wheat using diverse Ae. tauschii species with tetraploid wheat can be considered as a pleiotropic strategy to speed adaptive evolution of bread wheat in breeding processes by increasing both gene allele types and genetic recombination variations.

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