Characterization of fitness traits in thickspike wheatgrass

2008 ◽  
Vol 88 (5) ◽  
pp. 925-927 ◽  
Author(s):  
J. G. Robins ◽  
K. B. Jensen
Keyword(s):  
Seed Production ◽  
Biomass Production ◽  
Genotypic Correlation ◽  
Fitness Traits ◽  
Poor Seed ◽  
Elymus Lanceolatus ◽  
Genotypic Correlations ◽  
A Site ◽  
Key Words Biomass

Poor seed production and stand establishment are limitations to the use of thickspike wheatgrass [Elymus lanceolatus (Scribn. & J.G. Sm.) Gould] in rangeland re-vegetation projects. This study assessed the heritability of seed production, rhizome proliferation, and biomass production and genotypic correlations among these traits in a population of half-sib thickspike wheatgrass families at a site near Nephi, UT, USA during 2005 and 2006. Heritability estimates were ~ 0.6 for seed production and rhizome spread, but non-significant for biomass production. Genotypic correlations among the traits were all low or non-significant. Key words: Biomass production, genotypic correlation, heritability, rhizome, seed production, thickspike wheatgrass

Functional and Structural Characterization of Acquired Pan-Aminoglycoside Resistance 16S rRNA Methyltransferase NpmB1

2021 ◽  
Author(s):  
Akito Kawai ◽  
Masahiro Suzuki ◽  
Kentaro Tsukamoto ◽  
Yusuke Minato ◽  
Yohei Doi
Keyword(s):  
Amino Acid ◽  
16S Rrna ◽  
Amino Acid Identity ◽  
Single Amino Acid ◽  
Aminoglycoside Resistance ◽  
E Coli ◽  
The United Kingdom ◽  
Amino Acid Variant ◽  
A Site

Post-translational methylation of the A site of 16S rRNA at position A1408 leads to pan-aminoglycoside resistance encompassing both 4,5- and 4,6-disubstituted 2-deoxystreptamine (DOS) aminoglycosides. To date, NpmA is the only acquired enzyme with such function. Here, we present function and structure of NpmB1 whose sequence was identified in Escherichia coli genomes registered from the United Kingdom. NpmB1 possesses 40% amino acid identity with NpmA1 and confers resistance to all clinically relevant aminoglycosides including 4,5-DOS agents. Phylogenetic analysis of NpmB1 and NpmB2, its single amino acid variant, revealed that the encoding gene was likely acquired by E. coli from a soil bacterium. The structure of NpmB1 suggests that it requires a structural change of the β6/7 linker in order to bind to 16S rRNA. These findings establish NpmB1 and NpmB2 as the second group of acquired pan-aminoglycoside resistance 16S rRNA methyltransferases.

Hydrodynamic and isotopic characterization of a site contaminated by chlorinated solvents: Chienti River Valley, Central Italy

2013 ◽  
Vol 32 ◽  
pp. 164-174 ◽  
Author(s):  
Marco Petitta ◽  
Eva Pacioni ◽  
Chiara Sbarbati ◽  
Gianni Corvatta ◽  
Marco Fanelli ◽  
...  
Keyword(s):  
Central Italy ◽  
Chlorinated Solvents ◽  
River Valley ◽  
Isotopic Characterization ◽  
A Site

Processing and characterization of A-site deficient [(Ca, Sr) x (La, Nd)2/3−2x/3]TiO3 dielectric ceramics

2014 ◽  
Vol 25 (12) ◽  
pp. 5282-5287
Author(s):  
Muhammad Saleem ◽  
Yaseen Iqbal ◽  
Shan Qin ◽  
Xiang Wu ◽  
Shahid Ali ◽  
...  
Keyword(s):  
Dielectric Ceramics ◽  
A Site

Insects Affecting Seed Production in Red Pine: IV. Recognition and Extent of Damage to Cones

1957 ◽  
Vol 89 (6) ◽  
pp. 264-271 ◽  
Author(s):  
L. A. Lyons
Keyword(s):  
Seed Production ◽  
Production Capacity ◽  
Red Pine ◽  
Natural Control ◽  
Present Contribution ◽  
Southern Ontario ◽  
Pine Cone ◽  
Poor Seed ◽  
Extent Of Damage ◽  
Cone Damage

The broad study of factors responsible for poor seed production by red pines (Pinus resinosae Ait.) in central and southern Ontario has been concerned with the seed production capacity and efficiency of sound cones (1) and the description, life history, behaviour, and natural control of cone-inhabiting insects (2, 3, 4). The present contribution, which concludes the four-part series on red pine cone insects, deals with the time and duration of cone attack by the various insect species and the variability in the intensity of cone damage from year to year and from one locality to another. As an aid to the field diagnosis of the cause of cone damage, keys based on the appearance of mature larvae and injured cones are included.

Enzymatic Thioxyloside Synthesis: Characterization of Thioglycoligase Variants Identified from A Site-Saturation Mutagenesis Library ofBacillus CirculansXylanase

ChemBioChem ◽  
2010 ◽  
Vol 11 (4) ◽  
pp. 533-538 ◽  
Author(s):  
Zachary Armstrong ◽  
Stephan Reitinger ◽  
Terrence Kantner ◽  
Stephen G. Withers
Keyword(s):  
Saturation Mutagenesis ◽  
Site Saturation ◽  
A Site

scholarly journals The Yeast PH Domain Proteins Slm1 and Slm2 Are Targets of Sphingolipid Signaling during the Response to Heat Stress

2006 ◽  
Vol 27 (2) ◽  
pp. 633-650 ◽  
Author(s):  
Alexes Daquinag ◽  
Maria Fadri ◽  
Sung Yun Jung ◽  
Jun Qin ◽  
Jeannette Kunz
Keyword(s):  
Heat Stress ◽  
Signaling Pathways ◽  
Actin Polymerization ◽  
Mutational Analysis ◽  
Ph Domain ◽  
Calcium Calmodulin Dependent ◽  
Dependent Protein ◽  
A Site ◽  
Arginine Permease

ABSTRACT The PH domain-containing proteins Slm1 and Slm2 were previously identified as effectors of the phosphatidylinositol-4,5-bisphosphate (PI4,5P2) and TORC2 signaling pathways. Here, we demonstrate that Slm1 and Slm2 are also targets of sphingolipid signaling during the heat shock response. We show that upon depletion of cellular sphingolipid levels, Slm1 function becomes essential for survival under heat stress. We further demonstrate that Slm proteins are regulated by a phosphorylation/dephosphorylation cycle involving the sphingolipid-activated protein kinases Pkh1 and Pkh2 and the calcium/calmodulin-dependent protein phosphatase calcineurin. By using a combination of mass spectrometry and mutational analysis, we identified serine residue 659 in Slm1 as a site of phosphorylation. Characterization of Slm1 mutants that mimic dephosphorylated and phosphorylated states demonstrated that phosphorylation at serine 659 is vital for survival under heat stress and promotes the proper polarization of the actin cytoskeleton. Finally, we present evidence that Slm proteins are also required for the trafficking of the raft-associated arginine permease Can1 to the plasma membrane, a process that requires sphingolipid synthesis and actin polymerization. Together with previous work, our findings suggest that Slm proteins are subject to regulation by multiple signals, including PI4,5P2, TORC2, and sphingolipids, and may thus integrate inputs from different signaling pathways to temporally and spatially control actin polarization.

Isolation and characterization of dominant mutations resistant to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae

1981 ◽  
Vol 1 (2) ◽  
pp. 83-93
Author(s):  
K Matsumoto ◽  
A Toh-e ◽  
Y Oshima
Keyword(s):  
Partial Resistance ◽  
Carbon Catabolite Repression ◽  
Glucose Repression ◽  
Isocitrate Lyase ◽  
Isolation And Characterization ◽  
Enhanced Resistance ◽  
Leloir Pathway ◽  
Resistant Mutants ◽  
A Site

Seven dominant mutations showing greatly enhanced resistance to the glucose repression of galactokinase synthesis have been isolated from GAL81 mutants, which have the constitutive phenotype but are still strongly repressible by glucose for the synthesis of the Leloir enzymes. These glucose-resistant mutants were due to semidominant mutations at either of two loci, GAL82 and GAL83. Both loci are unlinked to the GAL81- gal4, gal80, or gal7 X gal10 X gal1 locus or to each other. The GAL83 locus was mapped on chromosome V at a site between arg9 and cho1. The GAL82 and GAL83 mutations produced partial resistance of galactokinase to glucose repression only when one or both of these mutations were combined with a GAL81 or a gal80 mutation. The GAL82 and GAL83 mutations are probably specific for expression of the Leloir pathway and related enzymes, because they do not affect the synthesis of alpha-D-glucosidase, invertase, or isocitrate lyase.

A Site Characterization Of The Soda Butte Creek Drainage, Yellowstone National Park

1996 ◽  
Author(s):  
Eric Nyberg ◽  
Wayne L. Hamilton ◽  
William R. Sill ◽  
Marvin A. Speece
Keyword(s):  
Yellowstone National Park ◽  
National Park ◽  
Site Characterization ◽  
A Site

scholarly journals Purification and Characterization of the R64 Shufflon-Specific Recombinase

2000 ◽  
Vol 182 (10) ◽  
pp. 2787-2792 ◽  
Author(s):  
Atsuko Gyohda ◽  
Teruya Komano
Keyword(s):  
Electrophoretic Analysis ◽  
Recombination Reaction ◽  
Site Specific ◽  
Dna Inversion ◽  
Repeat Sequences ◽  
In Vitro Recombination ◽  
Recombinase Gene ◽  
A Site

ABSTRACT The shufflon, a multiple DNA inversion system in plasmid R64, consists of four invertible DNA segments which are separated and flanked by seven 19-bp repeat sequences. The product of a site-specific recombinase gene, rci, promotes site-specific recombination between any two of the inverted 19-bp repeat sequences of the shufflon. To analyze the molecular mechanism of this recombination reaction, Rci protein was overproduced and purified. The purified Rci protein promoted the in vitro recombination reaction between the inverted 19-bp repeats of supercoiled DNA of a plasmid carrying segment A of the R64 shufflon. The recombination reaction was enhanced by the bacterial host factor HU. Gel electrophoretic analysis indicated that the Rci protein specifically binds to the DNA segments carrying the 19-bp sequences. The binding affinity of the Rci protein to the four shufflon segments as well as four synthetic 19-bp sequences differed greatly: among the four 19-bp repeat sequences, the repeat-a and -d sequences displayed higher affinity to Rci protein. These results suggest that the differences in the affinity of Rci protein for the 19-bp repeat sequences determine the inversion frequencies of the four segments.

Invasion Percolation Quantities on Correlated Networks

1998 ◽  
Vol 09 (06) ◽  
pp. 827-836 ◽  
Author(s):  
A. M. Vidales ◽  
E. Miranda ◽  
G. Zgrablich
Keyword(s):  
Heterogeneous Media ◽  
Front Velocity ◽  
Invasion Percolation ◽  
Final State ◽  
Bond Model ◽  
Correlation Degree ◽  
The Mean ◽  
A Site ◽  
Correlated Networks

Invasion percolation is studied on correlated square networks described through a site-bond model which has proven to be useful for the characterization of real heterogeneous media. It is shown how the correlation degree affects the mean front velocity, the number of islands of trapped defender fluid (which are completely surrounded by invaded elements), their size distribution and total number of steps to reach the final state. The correlation degree seems to affect the fractal dimension of the percolating cluster. A characteristic correlation length is found to exist which maximizes the mean invasion velocity.

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