Effects of bovine follicular fluid and luteinizing-hormone-releasing hormone antagonist on the dominant follicle maintained by a norgestomet ear implant in heifers

1995 ◽  
Vol 75 (1) ◽  
pp. 71-78 ◽  
Author(s):  
M. Manikkam ◽  
R. Rajamahendran ◽  
N. C. Rawlings
Keyword(s):  
Follicular Fluid ◽  
Implant Removal ◽  
Maximum Diameter ◽  
Saline Control ◽  
Control Group ◽  
Dominant Follicle ◽  
Serum Lh ◽  
Lhrh Antagonist ◽  
The Mean ◽  
Estradiol 17Β

The effects of BFF and LHRHa on the dominant follicle, maintained by 9-d synthetic progestin (norgestomet) ear implants inserted at proestrus, were studied in cattle. From day 4 of implant insertion, heifers (n = 18) were injected twice daily with saline (control, n = 6) or charcoal-extracted bovine follicular fluid, 10 mL i.v. for 4 d (BFF, n = 6) or LHRHa, 0.8 mg i.v. for 3 d (LHRHa, n = 6). Follicular changes were monitored daily by ultrasonography. Plasma estradiol-17β, serum LH and FSH were measured by radioimmunoassays. The dominant follicle was maintained during the treatment period, and it ovulated after implant removal in control heifers. In BFF group, the dominant follicle either ovulated after implant removal (two of six heifers) or gradually regressed until day 10 in the post-implant period (other four of six heifers). In the LHRHa group, the dominant follicle regressed during or after the implant period in 5 of 6 heifers. The dominant follicle ovulated in 1 of 6 heifers. Growth rate of the dominant follicle, until it reached its maximum diameter, was lower in BFF group than the control group (0.4 ± 0.1 vs 0.7 ± 0.1 mm d−1; P < 0.01). Both BFF and LHRHa groups showed high regression rates in those follicles that did regress (2.2 ± 0.1 (n = 4) and 1.9 ± 0.8 mm d−1 (n = 5) respectively; P < 0.01), compared with no regression in the control group. The BFF injections failed to suppress serum FSH levels consistently in all the heifers treated. LHRHa-treated heifers showed a significant decline (P < 0.01) in the mean serum LH concentration. In conclusion, deprivation of LH causes atresia of the dominant follicle. Key words: Follicular fluid, LHRH antagonist, follicular dynamics, gonadotropins, estradiol-17β, ultrasound, regression

129 Ovarian dynamics and gonadotropins during selection of the dominant follicle in postpartum lactating versus non-postpartum cycling mares

2020 ◽  
Vol 32 (2) ◽  
pp. 191
Author(s):  
M. Pastorello ◽  
M. O. Gastal ◽  
G. K. Piquini ◽  
D. B. Godoi ◽  
E. L. Gastal
Keyword(s):  
Growth Rate ◽  
Maximum Diameter ◽  
Sequential Data ◽  
Dominant Follicle ◽  
Follicle Diameter ◽  
The Mean ◽  
Low Levels ◽  
Follicle Selection ◽  
Ovarian Dynamics ◽  
Selection Of

The mare, compared to other livestock, has the shortest interval from partum to the first ovulation. In monovulatory species, the follicle deviation process in a wave is characterised by the continued growth of the dominant follicle (DF) and regression of the subordinate follicle. Although follicle diameter deviation, a key event of follicle selection, has been investigated during the oestrous and menstrual cycles, the occurrence of this phenomenon before the first postpartum ovulation seems to be unclear in all species. This study aimed to compare the follicular dynamics and gonadotropin profiles around the follicle diameter deviation day in postpartum lactating (PP Lactating; n=24) versus non-postpartum cycling (NPP Cycling; n=15) mares. On the day of parturition, every PP Lactating mare was paired with an NPP Cycling mare, and ovarian follicles (&gt;4mm) were tracked daily by transrectal ultrasonography, and blood samples were collected. Data were analysed in the PP Lactating group according to the length of the partum-ovulation interval (POI; ≤22 and &gt;22 days) and the postpartum interovulatory interval (PPIOI), and in the NPP Cycling group during two interovulatory intervals (1st and 2nd IOI). In addition, regardless of group, all four intervals were compared. We performed the FSH and LH assays using radioimmunoassay. Ovarian and hormonal parameters were analysed using ANOVA for sequential data. The day and diameter of the DF at the deviation (overall mean: 14.9±2.5 days; 21.7±0.4mm, respectively) were not different (P&gt;0.05) between PP Lactating and NPP Cycling mares. However, when considering the length of POI, follicle deviation occurred 4.4±0.8 days earlier (P&lt;0.001) in mares with POI ≤ 22 days than in mares with POI&gt;22 days. No difference was found between PP Lactating and NPP Cycling mares within and between groups for the intervals from deviation to maximum diameter of the DF (10.4±0.4 days), for the intervals from deviation to ovulation (12.1±0.5 days), or for the growth rates of the DF from deviation to maximum diameter (2.6±0.1mm per day). The growth rate of the DF from deviation to ovulation (2.4±0.1mm per day) did not differ between PP Lactating and NNP Cycling mares; however, this growth rate was lower (P&lt;0.03) in the POI, PPIOI, and 1st IOI compared with the 2nd IOI. The mean diameter of the DF around deviation (days −3 to 3; 22.5±0.3mm), and systemic FSH (days −4 to 4; 10.3±0.2ngmL−1) were not different between PP Lactating and NPP Cycling mares. Level of LH was lower (P&lt;0.0001) around deviation (days −4 to 4) in the PP Lactating (0.7±0.0ngmL−1) versus the NPP Cycling mares (1.8±0.1ngmL−1). Results demonstrated that a partum effect occurs only on the day of deviation in mares during the foal heat (POI ≤ 22 days) and that low levels of LH during both intervals in PP Lactating mares were not detrimental to prevent ovulation.

scholarly journals Endocrine Consequences of Long-Term Intrathecal Administration of Opioids

2000 ◽  
Vol 85 (6) ◽  
pp. 2215-2222 ◽  
Author(s):  
Roger Abs ◽  
Johan Verhelst ◽  
Jan Maeyaert ◽  
Jean-Pierre Van Buyten ◽  
Frank Opsomer ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
Intrathecal Administration ◽  
Lipoprotein Cholesterol ◽  
Control Group ◽  
Serum Lh ◽  
Nonmalignant Pain ◽  
The Mean

Intrathecal administration of opioids is a very efficient tool in the long-term control of intractable nonmalignant pain. However, despite the well known role of opioids in endocrine regulation, few data are available about possible effects on hypothalamic-pituitary function during this treatment. Seventy-three patients (29 men and 44 women; mean age, 49.2 ± 11.7 yr) receiving opioids intrathecally for nonmalignant pain were enrolled for extensive endocrine investigation. At the time of hormonal determination, the mean duration of opioid treatment was 26.6 ± 16.3 months; the mean daily dose of morphine was 4.8 ± 3.2 mg. The control group consisted of 20 patients (11 men and 9 women; mean age, 54.2 ± 14.0 yr) with a comparable pain syndrome but not treated with opioids. Decreased libido or impotency was present in 23 of 24 men receiving opioids. The serum testosterone level was below 9 nmol/L in 25 of 29 men and was significantly lower than that in the control group (P &lt; 0.001). The free androgen index was below normal in 18 of 29 men and was significantly lower than that in the control group (P &lt; 0.001). The serum LH level was less than 2 U/L in 20 of 29 men and was significantly lower than that in the control group (P &lt; 0.001). Serum FSH was comparable in both groups. Decreased libido was present in 22 of 32 women receiving opioids. All 21 premenopausal females developed either amenorrhea or an irregular menstrual cycle, with ovulation in only 1. Serum LH, estradiol, and progesterone levels were lower in the opioid group. In all 18 postmenopausal females significantly decreased serum LH (P &lt; 0.001) and FSH (P = 0.012) levels were found. The 24-h urinary free cortisol excretion was below 20 μg/day in 14 of 71 opioid patients and was significantly lower than that in the control group (P = 0.003). The peak cortisol response to insulin-induced hypoglycemia was below 180 μg/L in 9 of 61 opioid patients and was significantly lower than that in the nonopioid group (P = 0.002). The insulin-like growth factor I sd score was below −2 sd in 12 of 73 opioid patients and was significantly lower than that in the control group (P = 0.002). The peak GH response to hypoglycemia was below 3 μg/L in 9 of 62 subjects and was significantly lower than that in the control group (P = 0.010). Thyroid function tests and PRL levels were considered normal. No metabolic disturbances were recorded, apart from significantly decreased high density lipoprotein cholesterol levels (P = 0.041) and elevated total/high density lipoprotein cholesterol ratio (P = 0.008) in the opioid group compared to the control group. Supplementation with gonadal steroids improved sexual function in most patients. In conclusion, of all patients receiving intrathecal opioids, the large majority of men and all women developed hypogonadotropic hypogonadism, about 15% developed central hypocorticism, and about 15% developed GH deficiency. These findings suggest that further investigations are required to determine the need for systematic endocrine work-up in these patients and the necessity for substitutive therapy.

Effects of exogenous steroid hormones on the dominant follicle maintained by a Norgestomet implant in heifers

1994 ◽  
Vol 74 (3) ◽  
pp. 457-464 ◽  
Author(s):  
R. Rajamahendran ◽  
M. Manikkam
Keyword(s):  
Corn Oil ◽  
Pulse Amplitude ◽  
Follicular Phase ◽  
New Wave ◽  
Dominant Follicle ◽  
Serum Lh ◽  
Plasma Steroids ◽  
Follicle Atresia ◽  
Estradiol 17Β ◽  
Altered Serum

The maintenance of the dominant follicle during the follicular phase by a 9-d Norgestomet implant was used to study the effects of progesterone (P4), estradiol-17β (E2) and testosterone (T) on follicular turnover in heifers. From day 4 of Norgestomet treatment, heifers (n = 16) were injected daily for 4 d with corn oil (control), 150 mg P4, 10 mg E2 or 200 mg T (n = 4 per treatment). Follicular changes were monitored daily by ultrasonography. Plasma steroids and serum LH were measured by validated radioimmunoassays. In control heifers, dominant follicles were maintained during implantation and ovulated after implant removal. In both P4- and T-treated heifers, dominant follicles began to regress during the implant period and the dominant follicle from a new wave of follicles ovulated. In E2-treated heifers, dominant follicles regressed (3/4) and dominant follicles from the next wave ovulated. The dominant follicle became cystic in the fourth E2-treated heifer. Mean serum LH levels were suppressed (P < 0.01) from 0.55 ± 0.01 ng mL−1 (mean ± SEM) in control to 0.21 ± 0.02, 0.19 ± 0.02 and 0.43 ± 0.02 in P4-, E2- and T-treated heifers, respectively. The number of LH pulses in 8 h was reduced (P < 0.01) from 6.25 ± 0.75 in control to 2.00 ± 1.00 and 4.66 ± 0.35 in P4- and E2-treated heifers but not in T-treated heifers (6.33 ± 1.44). LH pulse amplitude (ng mL−1) was also suppressed (P < 0.01) in P4-treated (0.10 ± 0.04) and E2-treated (0.09 ± 0.02) heifers when compared with control heifers (0.41 ± 0.04) but not in T-treated heifers (0.42 ± 0.17). Exogenous P4 and E2 caused regression of the Norgestomet-maintained dominant follicle, and this effect was associated with altered serum LH profile. Injection of T caused atresia of the dominant follicle without altering the LH profile. Key words: Dominant follicle, atresia, heifers, steroids, LH, ultrasound, E2, estradiol-17β; P4, progesterone; T, testosterone

scholarly journals A Clinical Trial on the Effects of Different Regimens of Phenylephrine on Maternal Hemodynamic After Spinal Anesthesia for Cesarean Section

2020 ◽  
Vol 10 (4) ◽  
Author(s):  
Mahshid Nikooseresht ◽  
Mohammad Ali Seifrabiei ◽  
Pouran Hajian ◽  
Shadab Khamooshi
Keyword(s):  
Blood Pressure ◽  
Clinical Trial ◽  
Cesarean Section ◽  
Spinal Anesthesia ◽  
Cesarean Delivery ◽  
Saline Control ◽  
Control Group ◽  
Significant Difference ◽  
The Mean ◽  
Phenylephrine Infusion

Background: Phenylephrine is used to prevent and treat hypotension during spinal anesthesia for cesarean delivery. Objectives: The present study aims to investigate the effects of different regimens of phenylephrine on blood pressure of candidates for the cesarean section. Methods: In this double-blind, randomized clinical trial, a total of 120 candidates of elective cesarean delivery under spinal anesthesia was randomly categorized into three groups. Groups 1, 2, and 3 received bolus and prophylactic saline (control group), prophylactic bolus phenylephrine (100 µg), and prophylactic phenylephrine infusion (50 µg/min), respectively. The incidence of hypotension, maternal hemodynamics, hypertension, rescue phenylephrine dose, nausea, and vomit were compared between the groups. Results: In all the three groups, the incidence of nausea and vomit, bradycardia, hypertension, and neonatal Apgar score were not statistically different (P > 0.05). However, the adjuvant phenylephrine dose requirement was remarkably different. Moreover, the mean systolic blood pressure differed significantly in the second and 7th minutes after the spinal block (P < 0.05). 35% in the first group, 15% in the second group, and 2.5% in the third group had hypotension (P = 0.001). Apart from the first measurement after spinal anesthesia, the mean heart rate showed no significant difference between the groups. Conclusions: The use of prophylactic phenylephrine infusion is recommended to control the optimal blood pressure in parturients undergoing cesarean section after spinal anesthesia.

ABSENCE OF INHIBITION BY HYPERPROLACTINAEMIA ON OVARIAN RESPONSE TO EXOGENOUS GONADOTROPHIN

1978 ◽  
Vol 89 (1) ◽  
pp. 142-148 ◽  
Author(s):  
Toshihiro Aono ◽  
Motoi Yasuda ◽  
Takenori Shioji ◽  
Kunio Kondo ◽  
Keiichi Kurachi
Keyword(s):  
Menstrual Cycle ◽  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Ovarian Response ◽  
Serum Levels ◽  
Control Group ◽  
Serum Lh ◽  
Human Menopausal Gonadotrophin ◽  
Injection Control ◽  
The Mean

ABSTRACT In order to assess the effect of hyperprolactinaemia on the ovarian response to exogenous gonadotrophin, serum oestrogen levels were determined in 6 normal females. Two hundred and twenty-five IU of human menopausal gonadotrophin (hMG) was im injected daily for 3 days from the 4th day of the menstrual cycle, and the serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL) and oestradiol-17β were determined by radioimmunoassay daily for 7 days starting from the first day of injection (control cycle). After 2 months the same schedule was applied to the previous 6 subjects and in addition sulpiride 100 mg bid was given orally during the course of the study (sulpiride cycle). There was a significant increase in serum FSH and a decline in serum LH during hMG treatment in both groups. The mean (± se) serum levels of PRL in the sulpiride group increased gradually from 24.5 ± 3.8 ng/ml (1st day) to 56.2 ± 3.4 ng/ml (7th day). All these levels were significantly higher than those of the control group. The mean (± se) serum oestradiol increments by hMG stimulation in control and sulpiride groups showed a peak on the 5th day with respective levels of 757.2 ± 202.3 and 845.3 ± 263.3 pg/ml. No significant differences in the mean oestradiol increment were found between the two groups on any day. These results indicate that acute hyperprolactinaemia does not appear to induce ovarian refractoriness to exogenous gonadotrophin in normal cyclic women.

The interrelationship between the occurrence of oversized follicles and the peripheral and intra-follicular concentrations of E2, P4, FSH, and LH in female dromedary camels

2019 ◽  
Keyword(s):  
Luteinizing Hormone ◽  
Follicular Fluid ◽  
Follicle Stimulating Hormone ◽  
Pathological Finding ◽  
Control Group ◽  
Dromedary Camels ◽  
Thin Walls ◽  
Manual Palpation ◽  
Follicle Aspiration ◽  
Estradiol 17Β

The present study aimed to clarify the phenomenon of presence of larger than normal follicles (OVGF) in female dromedary camels. Females with OVGF (n=125) were examined by manual palpation and ultrasonography. Accordingly, the OVGF were subdivided into those with thin walls and clear hypoechogenic content (OVGF-TH, n=18) and those with thick walls and fibrous trabeculae (OVGF-TK, n=107). Transvaginal follicle aspiration was performed in females with OVGF and from a control group with growing follicles (1-2 cm in diameter, GF group, n=5). Serum was collected at the same time of follicle aspiration and analyzed for Follicle-stimulating hormone (FSH), luteinizing hormone (LH), progesterone (P4) and estradiol-17β profiles (E2). The follicular fluid (FF) was analyzed for E2 and P4. The results showed that mean E2 concentration in FF and serum were lower in OVGF-TH and OVGHTK groups than in the GF group (P < 0.05). Difference between OVGF-TH and OVGH-TK groups was not significant. P4 in FF did not significantly differ among groups. Positive correlation was found between E2 in FF and E2 in serum (r = 0.495, r = 0.03). Mean FSH concentration in serum was higher in OVGF-TH and OVGH-TK groups than in the GF group (P = 0.03). Mean LH concentration was non-significantly (P=0.1) greater in OVGF-TH and OVGH-TK groups than in the GF group. In conclusion, female dromedary camels with OVGF had endocrine characteristics differed from camels with no OVGF. It seems that the high FSH and/or LH concentration(s) stimulated the continuing growth of the developing follicles to reach these large sizes, suggesting that the phenomenon of OVGF in camels is a pathological finding.

Ultrasonographic Studies of Follicular Activities in Clomiphene Citrate Treated Anoestrus Cows

2016 ◽  
Vol 12 (2) ◽  
Author(s):  
Manjusha Patil ◽  
M. V. Ingawale ◽  
H. S.. Birade ◽  
A. Syed Anwar
Keyword(s):  
Growth Rate ◽  
Treatment Group ◽  
Estrous Cycle ◽  
Drug Administration ◽  
Clomiphene Citrate ◽  
Maximum Diameter ◽  
Control Group ◽  
Ultrasound Scanning ◽  
Dominant Follicle ◽  
Ovulatory Follicle

An experiment on ultrasonographic studies of follicular activities in clomiphene citrate treated anoestrus cows was conducted. Ultrasound scanning was performed to record the follicular activities on alternate day throughout one estrous cycle for control group and from drug administration to induction of estrus in treatment group. The postpartum induced estrus (treatment group) and regular estrus (control group) differed highly significantly (p is less than 0.01) with respect to number of follicles involved in ovulatory wave (6.67 ± 0.21 Vs 5.00 ± 0.45) and significantly (pis less than 0.05) with respect to growth rate (1.82 ± 0.34 Vs 1.04 ± 0.94 mm/day) and increase in diameter of dominant follicle (7.58 ± 0.72 Vs 10.2 ± 0.47 mm). Maximum diameter of ovulatory follicle in induced estrus was greater than regular estrus (16.12 ± 0.56 Vs15.18 ± 0.47 mm), however, it differed non-significantly

scholarly journals Effects of methylene blue on postoperative low-back pain and functional outcomes after lumbar open discectomy: a triple-blind, randomized placebo-controlled trial

2016 ◽  
Vol 24 (1) ◽  
pp. 7-15 ◽  
Author(s):  
Majid Reza Farrokhi ◽  
Mehrzad Lotfi ◽  
Mohammad Sadegh Masoudi ◽  
Mehrnaz Gholami
Keyword(s):  
Methylene Blue ◽  
Low Back Pain ◽  
Back Pain ◽  
Postoperative Pain ◽  
Radicular Pain ◽  
Saline Control ◽  
Control Group ◽  
Low Back ◽  
Pain Scores ◽  
The Mean

OBJECT Despite advances in surgical and anesthesiology techniques, many patients continue to experience postoperative pain after lumbar disc operations. This study aims to investigate the effects of methylene blue (MB) on preventing postoperative low-back pain (LBP) with or without radicular pain and improving the quality of life (QOL) in patients undergoing lumbar open discectomy. METHODS This is a prospective, randomized, triple-blind, placebo-controlled clinical trial, which was conducted at Shiraz University of Medical Sciences between July 2011 to January 2012. Of a total of 130 patients, 115 were eligible for participation; 56 received 1 ml of MB solution at a concentration of 0.5% (MB group) and 59 received an equivalent volume of normal saline (control group). Primary outcomes were the control of LBP with or without radicular pain, which was evaluated preoperatively and at 24 hours and 3 months after surgery with the use of a visual analog scale (VAS), and the improvement of QOL, which was assessed preoperatively and 3 months postoperatively by means of the Persian translation of the Oswestry Disability Index questionnaire. RESULTS The mean VAS scores for LBP were significantly lower in the MB group compared with the control group at 24 hours (1.25 ± 0.97 vs 2.80 ± 0.69, p < 0.001) and 3 months (1.02 ± 1.29 vs 2.07 ± 1.10, p = 0.019) after treatment. The mean radicular pain scores decreased significantly in the 2 groups at 24 hours after surgery, but the mean radicular pain score was significantly lower in the MB-treated patients than the control group. However, the difference between radicular pain scores in the MB group (1 ± 1.1) and the control group (1.2 ± 1) was not statistically significant (p = 0.64). The reduction in LBP was greater in the MB group than the control group (8.11 ± 1.74 vs 6.07 ± 1.52, p = 0.023, CI 95% −1.37 to −0.10). The functional QOL improved significantly 3 months after the operation in both groups (p < 0.001). Moderate disability occurred more frequently in the control group than in the MB group (14.5% vs 7.7%, p = 0.004). No toxicity, adverse effects, or complications were found in the group of patients treated with MB injection. CONCLUSIONS A single dose of MB (1 ml 0.5%) for coating the dura and surrounding tissues (facet and muscle) shows promising results in terms of safety, reduction of postoperative pain, and functional outcome compared with placebo.

Vitamin D Treatment in Patients with Hashimoto’s Thyroiditis may Decrease the Development of Hypothyroidism

2016 ◽  
Vol 86 (1-2) ◽  
pp. 9-17 ◽  
Author(s):  
Bekir Ucan ◽  
Mustafa Sahin ◽  
Muyesser Sayki Arslan ◽  
Nujen Colak Bozkurt ◽  
Muhammed Kizilgul ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Hashimoto’S Thyroiditis ◽  
Healthy Control Group ◽  
Hashimoto's Thyroiditis ◽  
Control Group ◽  
Endocrine Society ◽  
Thyroid Autoantibody ◽  
Healthy Control ◽  
The Mean

Abstract.The relationship between Hashimoto’s thyroiditis and vitamin D has been demonstrated in several studies. The aim of the present study was to evaluate vitamin D concentrations in patients with Hashimoto’s thyroiditis, the effect of vitamin D therapy on the course of disease, and to determine changes in thyroid autoantibody status and cardiovascular risk after vitamin D therapy. We included 75 patients with Hashimoto’s thyroiditis and 43 healthy individuals. Vitamin D deficiency is defined as a 25-hydroxy vitamin D (25(OH)D3) concentration less than 20ng/mL. Vitamin D deficient patients were given 50.000 units of 25(OH)D3 weekly for eight weeks in accordance with the Endocrine Society guidelines. All evaluations were repeated after 2 months of treatment. Patients with Hashimoto’s thyroiditis had significantly lower vitamin D concentrations compared with the controls (9.37±0.69 ng/mL vs 11.95±1.01 ng/mL, p < 0.05, respectively). Thyroid autoantibodies were significantly decreased by vitamin D replacement treatment in patients with euthyroid Hashimoto’s thyroiditis. Also, HDL cholesterol concentrations improved in the euthyroid Hashimoto group after treatment. The mean free thyroxine (fT4) concentrations were 0.89±0.02 ng/dL in patients with Hashimoto’s thyroiditis and 1.07±0.03 ng/dL in the healthy control group (p < 0.001). The mean thyroid volumes were 7.71±0.44 mL in patients with Hashimoto’s thyroiditis and 5.46±0.63 mL in the healthy control group (p < 0.01). Vitamin D deficiency is frequent in Hashimoto’s thyroiditis and treatment of patients with this condition with Vitamin D may slow down the course of development of hypothyroidism and also decrease cardiovascular risks in these patients. Vitamin D measurement and replacement may be critical in these patients.

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