Phosphorus-31 nuclear magnetic resonance spectroscopy of liver from two broiler chicken lines
Hepatic levels of phosphorus-containing compounds that are related to fat metabolism in the liver of two broiler lines with different body-fat content were assessed using high-resolution 31P NMR spectroscopy. PCA extracts of livers from 16-d-old chicks were analyzed with an NMR spectrometer. The results demonstrated that the concentrations of G3P, AMP and phosphocholine were significantly higher in the livers of LL birds than in those of the FL birds (P < 0.05). Differences in hepatic concentration of GPE and GPC were not detected between the two lines. It was also found that the liver weight per unit body weight of the FL chickens was less than that of the LL birds (P < 0.01). Average body weight of FL birds at 16 d of age was lighter than that of LL birds (P = 0.01). The FL birds deposited more (P < 0.05) fat per unit body weight than the LL birds. However, no statistical difference (P > 0.05) was found in protein deposition per unit body weight between the two lines. These results indicate that liver size and the concentration of hepatic phosphorus containing compounds are related to the partition of energy in broiler chickens. Key words: NMR spectroscopy, phosphorus-containing compounds, fat deposition, liver, broiler chicken