Feed intake and digestion by Holstein steer calves consuming low-quality grass supplemented with lasalocid or monensin

1993 ◽  
Vol 73 (4) ◽  
pp. 869-879 ◽  
Author(s):  
D. L. Galloway Sr. ◽  
A. L. Goetsch ◽  
A. Patil ◽  
L. A. Forster Jr. ◽  
Z. B. Johnson

Holstein steer calves were fed late vegetative bermudagrass or mature bromegrass free choice in three Latin-square experiments to determine influences of ionophore type and level, supplementation with cereal grain, and source of low-quality grass on feed intake and digestion. In exp. 1, neither lasalocid nor monensin given at 0.5 mg kg−1 of body weight (BW) affected intake or digestion with either forage source. In exp. 2, steers received lasalocid or monensin at 0, 0.5 or 1.0 mg kg−1 of BW. Neutral detergent fiber digestibility was not depressed by ionophores and was slightly greater (P < 0.05) for ionophores at 1.0 than at 0.5 mg kg−1 of BW. Digestible organic matter intake with bermudagrass was slightly greater with ionophores at 1.0 than at 0.5 mg kg−1 of BW, but an opposite difference between ionophore levels occurred with bromegrass (ionophore level × forage source interaction; P < 0.07). The concentration of total volatile fatty acids in rumen fluid at 4 h post-feeding was lower (P < 0.05) for monensin than for lasalocid; the acetate/propionate ratio was decreased by ionophores and was lower for monensin than for lasalocid (P < 0.05). In exp. 3, lasalocid at 0.5 mg kg−1 of BW did not affect intake or digestion or interact with supplemental corn (7.2 g kg−1 of BW) or forage source. In conclusion, level of ionophore maximizing digestible organic matter intake may vary with characteristics of source of low-quality forage. However, when present, effects of ionophores on digestibility or energy intake may be relatively small. Key words: Cattle, ionophore, forage, intake, digestion

2003 ◽  
Vol 76 (2) ◽  
pp. 319-326
Author(s):  
J. van E. Nolte ◽  
A.V. Ferreirat ◽  
H.H. Köster

AbstractA 5 ✕ 5 Latin square was conducted with ruminally and duodenally cannulated Dohne Merino wethers consuming wheat straw to determine the effect of different rumen degradable protein (RDP) levels on forage intake, fermentation characteristics, nutrient flow and digestion. The wethers had ad libitum access to water and wheat straw (32 g crude protein (CP) per kg dry matter (DM); 742 g neutral-detergent fibre (NDF) per kg DM) that was offered twice daily, immediately after intraruminal infusion of the supplements at 07:00 and 19:00 h. The supplemental RDP (calcium caseinate; 900 g CP per kg DM) levels were: 0, 40, 80, 120 and 160 g/day. Each period consisted of 14 days of adaptation and 6 days of sampling. Forage and total organic matter (OM) intakes increased in a linear manner (P < 0•01) with increasing supplemental RDP levels. Digestible organic matter intake (DOMI) displayed a quadratic increase with elevated amounts of RDP (P < 0•01). The effects of treatments on rumen and total tract digestion, as well as fluid dilution rate were minimal. Microbial nitrogen (MN) flow to the duodenum and microbial efficiency increased quadratically (P < 0•04) with increased RDP supplementation. Rumen ammonia nitrogen (NH3-N) concentrations increased linearly (P < 0•01) and total volatile fatty acids (VFA) tended to increase linearly (P = 0•07). In conclusion, RDP supplementation to Dohne Merino wethers consuming wheat straw generally enhanced rumen fermentation and forage intake. A total RDP intake (sources: calcium caseinate and wheat straw) of 3•30 g/kg M0•75 or 0•12 of DOM maximized DOMI.


1980 ◽  
Vol 94 (1) ◽  
pp. 219-227 ◽  
Author(s):  
C. G. Wernli ◽  
R. J. Wilkins

SummaryGrass silage (21.0 % D.M.) was given ad libitum to sheep together with supplements of rolled barley, dried-grass pellets or dried-grass wafers at 12 and 25 g organic matter (OM)/kg0·75.Intake of silage was greater with supplement at 12 g/kg0·75 but total intake of OM and digestible organic matter (DOM) increased with increasing supplement. Intake of OM was higher with the pellet supplement than with other supplements. Intake of DOM, however, did not differ between the pellet and barley treatments, but was lower with wafers.Concentrations of ammonia and total volatile fatty acids and the molar proportions of butyric and higher volatile fatty acids were higher when silage was supplemented with barley than with dried grass. Mean rumen retention times of silage and of supplement were highest in the barley-supplemented treatments. Dried-grass wafers were retained longer than dried-grass pellets. Supplement treatments did not differ significantly for eating and ruminating times, rumen fluid volume and pH, digestion rate in the rumen and nitrogen retention.At the low supplement rate silage consumption may have been controlled by factors associated directly with the silage, whereas at the high rate intake was limited either by physical factors or by the potential energy demand of the animals.


2001 ◽  
Vol 41 (2) ◽  
pp. 155 ◽  
Author(s):  
I. W. Karda ◽  
G. McL. Dryden

Tarramba leucaena (Leucaena leucocephala cv. Tarramba) foliage had per kilogram dry matter, 169 g protein and 29.8 g condensed tannins. Its value as a supplement, given either with or without urea, to sheep given a low-quality Callide Rhodes grass (Chloris gayana cv. Callide) hay was studied. Six rumen fistulated sheep (mean s.d. liveweight, 34 1.4 kg) were used to compare 6 dietary treatments in an incomplete latin square design. Rhodes grass hay was given ad libitum either alone, or with urea 7 g/day (U), or with leucaena 150 g/day (L150), or leucaena with urea (L150U), or leucaena 300 g/day (L300), or leucaena with urea (L300U). Digestible organic matter intake was increased significantly by leucaena supplementation although digestibility of the whole diet did not alter. Rumen fluid ammonia-N was not altered by leucaena supplementation, but was increased by urea. This suggests that Tarramba foliage protein has some resistance to ruminal degradation. Liquid and solids passage rates were not affected by the treatments. Microbial nitrogen supply to the intestine (g/day), and the efficiency of microbial nitrogen synthesis (g/kg organic matter apparently digested in the rumen), were increased by leucaena supplementation (P<0.01). Microbial protein synthesis was the only response in which 300 g/day air-dry Tarramba foliage gave improved results over 150 g/day.


2009 ◽  
Vol 38 (5) ◽  
pp. 948-955 ◽  
Author(s):  
Marinaldo Divino Ribeiro ◽  
José Carlos Pereira ◽  
Augusto César de Queiroz ◽  
Vitor Pereira Bettero ◽  
Hilário Cuquetto Mantovani ◽  
...  

This study aimed to evaluate the effect of intraruminal infusion of propionic acid (RPA) associated to two (80:20 and 60:40) forage:concentrate ratios (FCR), for young bulls. Four Holstein × Zebu crossbred young bulls with average age of 7 months and 160 kg of body weight (BW) were distributed to a 4 × 4 Latin square design. A total mixed ration, isonitrogenous diets, was ad libitum fed to animals. The propionic acid was obtained by fermentation of reconstituted milk whey by the consortium of bacteria Enterococcus sp. and Veillonella sp.. Daily and along with the supply of solid feeds, 2 L of the compound containing propionic acid were intrainfused in the rumen. There was no RPA × FCR interaction for the intake of nutrients, except for total digestible nutrients (TDN). The organic matter (OM), crude protein (CP), ether extract (EE), neutral detergent fiber (NDF) and TDN intakes were influenced by the FCR. There was no RPA × FCR interaction on the total digestibility of DM, OM, CP, EE, NDF and TC. However, the digestibility of DM, OM, EE and CT were influenced by the FCR. There was no RPA × FCR interaction for the mean N-NH3 concentration and pH of the rumen fluid; however, RPA provided effect on the pH measured 4 hours after meal. There was no interaction or effect of the main factors on volatile fatty acids concentrations in the rumen fluid, or on glucose levels and serum urea nitrogen.


2000 ◽  
Vol 70 (2) ◽  
pp. 325-333
Author(s):  
N.W. Offer

AbstractTwo silages were made from a regrowth of perennial ryegr ass following a 24-h wilt using either 2·5 l/t (LF) or 5·5 l/t (HF) of Add Safe (an aqueous solution of ammonium tetraformate, Trouw Nutrition, Cheshire, UK). Composition of silages LF and HF respectively were for dry matter (DM, g/kg) 205, 217; crude protein (g/kg DM) 106, 104; digestible organic matter (g/kg DM) 665, 692, pH 3·8, 3·9; lactic acid (g/kg DM) 103, 84; neutralizing value (meq/kg DM) 1051, 892; acetic acid (g/kg DM) 18·4, 14·0. Experiment A measured the intakes of the two silages using 12 Scottish Blackface wether lambs (mean live weight 25 kg) using a cross-over design with periods of 21 days. Intakes (g DM per day) were significantly greater (P < 0·05) for silage HF (329) than for LF (268). Experiment B measured the voluntary intakes of dried silage residues following juice extraction using the same protocol as for experiment A, except that only six lambs were used. There were no significant differences in composition or intake (P > 0·05) of the dried residues from the two silages. Experiment C used a 5 × 5 Latin-square design to measure the effects on voluntary intake of adding juices from the two silages to unmolassed sugar-beet nuts (SBN) pre-feeding or administered directly into the rumen in two discrete doses per day. Sheep on treatments LED and HFD received juices added to SBN (1·5 l/kg) and water added into the rumen in equal volume to the juice consumed with the previous day’s SBN. Treatments LFR and HFR received water added to the SBN (1·5 l/kg) and juice into the rumen in equal volume to the water consumed with the previous day’s SBN. The control treatment (W) was water added both to the SBN and into the rumen. Total mean daily intakes (g/day) were 1930,1954,1296, 2034 and 1703 (s.e.d. 179) for treatments LED, HFD, LFR, HFR and W respectively. Treatment LFR gave significantly lower mean daily intakes (P < 0·05) than all other treatments. Values for pH, volatile fatty acids and ammonia in the rumen fluid were not significantly affected by treatment (P > 0·05). Differences in voluntary intake for the two silages made from the same crop using different ensilage methods were attributed to differences in the silage liquid and volatile phases and not to the non-volatile solid phase. The effects of juice composition on intake were greater when added directly to the rumen than when added to the diet suggesting that effects on taste or smell were relatively unimportant.


2019 ◽  
Vol 59 (9) ◽  
pp. 1660 ◽  
Author(s):  
Pok Samkol ◽  
Keo Sath ◽  
Mikaela Patel ◽  
Mom Seng ◽  
Kjell Holtenius

Four adult crossbred cattle, fitted with rumen fistula in the dorsal sac, were randomly allocated to four treatments in a 4 × 4 Latin-square design to study the effects of supplementing sun-dried groundnut (Arachis hypogea L.) foliage (DGF) and sun-dried cassava (Manihot esculenta, Crantz) tops (DCT) on feed intake, apparent digestibility and rumen fermentation. All animals were fed a basal diet of rice straw ad libitum and para grass (Brachiaria mutica) at 1% of liveweight (LW). The four experimental diets were as follows: (1) control, basal diet without supplement; (2) DGF, basal diet + DGF; (3) DCT, basal diet + DCT; and (4) DGF+DCT, basal diet + DGF + DCT. The supplementary feeds were fed to the level of 2 g crude protein (CP)/kg LW in Diets 2 and 3 and 1 g CP/kg LW of each feed in Diet 4. The results indicated that total intake of dry matter (DM), organic matter and CP increased with DGF and DGF+DCT diets, while neutral detergent fibre (NDF) intake was highest in the DGF diet. Digestibilities of DM, organic matter, CP and NDF were improved in DGF and DGF+DCT treatments. However, the diet with DCT supplement had lowest CP and NDF digestibility. Ruminal NH3-N and total volatile fatty acids increased in all diets with supplements, but ruminal pH, volatile fatty acid profile and in sacco losses of DM, CP and NDF of sun-dried para grass in the rumen did not differ among the treatments.


2017 ◽  
Vol 57 (1) ◽  
pp. 129 ◽  
Author(s):  
A. K. S. Schulze ◽  
A. C. Storm ◽  
M. R. Weisbjerg ◽  
P. Nørgaard

The major microbial fermentation of forages and production of volatile fatty acids (VFA) takes place in the medial part of the rumen, whereas the absorption of VFA occurs through the rumen epithelium, for example the ventral sac. The objective was to study effects of forage neutral detergent fibre (NDF) content and time after feeding on the medial to ventral VFA and pH gradient as well as rumen motility in the rumen of heifers fed grass/clover silages. Four silages were harvested at different growth stages with NDF contents of 31–45% of DM and in vitro organic matter digestibilities of 75–82% and fed to four rumen-fistulated Jersey heifers at 90% of ad libitum level in a Latin square design, with half the ration fed at 0800 hours and 1530 hours. Rumen fluid was sampled hourly from 0730 hours to 1530 hours in the medial and ventral rumen, and analysed for pH and concentrations of VFA, L-lactic acid, and ammonia to assess ruminal chemical gradient. Reticular contractions were continuously recorded by a pressure transducer. Time relative to feeding affected rumen parameters as pH was generally lower and VFA content greater in medial compared with ventral rumen fluid. Greater NDF content of the silage caused lower VFA concentration and higher pH in the rumen mat, and therefore the gradient diminished at greater NDF content in the silages; an effect probably caused by reduced organic matter digestibility rather than digesta NDF properties. This study therefore suggests that VFA production decreased with greater NDF content of forages, whereas intra-ruminal equilibration increased.


1976 ◽  
Vol 86 (1) ◽  
pp. 163-169 ◽  
Author(s):  
T. N. Barry ◽  
P. D. Johnstone

SUMMARYChopped barley straw was fed ad libitum to penned Romney wether sheep. Supplements of urea, ground wheat grain + urea, lucerne hay and autumn saved pasture (ASP) were given for five periods each of 3 weeks using a 5 × 5 Latin square design. The latter three supplements supplied similar amounts of nitrogen and approximately 20% of the energy required for maintenance.The concentration of ammonia in the rumen fluid of sheep fed the basal straw diet was low and was increased by all the supplements. The concentration of total volatile fatty acids (VFA) in rumen fluid was unaffected by supplementation with urea, but was increased by the three supplements of nitrogen and energy. Differences in VFA molar proportions between treatments were small.Sheep offered straw alone had a digestible energy (DE) intake of 69% of maintenance and showed a weight loss of 121 g/day. Supplementation with urea had no effect upon apparent energy digestibility (42%) or voluntary intake, but significantly reduced the rate of live-weight loss. The lucerne hay and ASP supplements also had no effect upon straw consumption, but increased total DE intake to 87% of maintenance due to the additional energy provided by the supplements, and reduced live-weight loss. Supplementation with wheat + urea increased the estimated amount of digestible organic matter derived from straw by 8·5%, increasing DE intake to 94% of maintenance and reducing live-weight loss to 18 g/day.Averaged over all five groups of animals, straw intake increased by 9% in the second period of feeding and then gradually decreased with time, reaching a value in period 5 which was similar to period 1.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 377-377
Author(s):  
Thomas G Jennings ◽  
Angela Green-Miller

Abstract The objective was to evaluate treatments designed to increase passage rate on forage intake and digestion. Eight ruminally-cannulated steers were assigned to a replicated 4 × 4 Latin square design. All steers were fed an ad libitum diet of prairie grass hay (10.4% CP) with the following treatments designed to increase passage rate: no treatment (control; CON), pelleted (PEL), 7% Ca(OH)2 treated (CAOH), and six 2 kg weights inserted into the rumen (WTS). Molasses (3% of diet DM) was added to all treatments. Feed, orts, and total feces was collected on d 12–17 to determine total tract digestibility. On d 17–20, ruminal pH was recorded every 8 hr. Data were analyzed using the MIXED procedure of SAS 9.4 with treatment and period as fixed effects and steer as a random effect. A treatment effect (P &lt; 0.01) was observed for ruminal pH; steers fed CAOH had the highest pH (6.97) with WTS and CON intermediate and PEL being the lowest (6.34). Organic matter intake (OMI) was affected (P &lt; 0.01) by treatment with PEL steers consuming the most (7.86 kg). While steers consuming CON had greater OMI than CAOH (P = 0.04), WTS was not different from CON (P = 0.50). Organic matter digestibility (OMD) was also affected (P &lt; 0.01) by treatment as WTS and CON were the greatest (62.8% and 61.7%, respectively) and CAOH (58.7%) tended (P = 0.08) to be greater than PEL (55.9%). Total digestible organic matter intake (TDOMI) was affected (P &lt; 0.01) by treatment; PEL had the greatest TDOMI with CON being intermediate, and CAOH and WTS being the least. Treatments designed to increase passage rate did affect forage intake and digestion with the greatest effect resulting from pelleting grass hay due to the severe reduction in particle size.


1972 ◽  
Vol 79 (1) ◽  
pp. 99-103 ◽  
Author(s):  
A. M. Raven

SUMMARYA 6 x 6 Latin Square balance experiment was carried out using six Friesian steers, each of which initially weighed about 304 kg. The six treatments studied were an all-hay diet and five other diets containing 20,40,60,80 and 100 % of rolled barley fortified with mineral and vitamin supplements, accompanied by correspondingly reduced proportions of hay. Each diet was fed at an estimated maintenance level of feeding.The progressive increase in the proportion of concentrate gave a significantly linear increase (P < 0·001) in both digestible and calculated metabolizable energy. The actual increase in digestible energy was from 2·62Mcal/kg dry matter (59·3% of the gross energy) on the all-hay treatment to 3·42 Mcal/kg dry matter (79·5% of the gross energy) on the all-concentrate treatment. Use of the determined digestible energy values for the all-hay and fortified barley diets to calculate the digestible energy of the four mixed diets gave results in reasonably good agreement with the determined values, the maximum difference being 0·12 Mcal/kg dry matter, which represented 3·83 % of the determined value. The losses of energy in the urine expressed as percentages of the gross energy of the diets showed a small but significantly linear decrease (P < 0·01) with increase in proportion of barley in the diet. The molar proportions of steamvolatile acids in samples of rumen fluid taken from two animals on each treatment indicated that increase in the proportion of concentrate was associated with tendencies for increase in acetic acid, decrease in propionic acid and little change in butyric acid. The mean digestibility of the organic matter was 62·6 % on the all-hay treatment and 81·8 % on the all concentrate treatment. The progressive increase in the proportion of concentrate gave a significantly linear increase (P < 0·001) in digestibility of the organic matter. Although intakes of nitrogen decreased with increase in the proportion of concentrate due to a decrease in the amount of dry matter fed, the weights of nitrogen retained were well maintained and when expressed as percentages of intake showed a significantly linear increase (P < 0·01).


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