Effect of supplementary energy and protein feeding on the true digestion of grass-silage organic matter, cell walls and nitrogen estimated by the combined synthetic-fibre-bag method in cows

1992 ◽  
Vol 72 (3) ◽  
pp. 671-678 ◽  
Author(s):  
Tuomo Varvikko ◽  
Aila Vanhatalo
Keyword(s):  
Organic Matter ◽  
Cell Walls ◽  
Latin Square ◽  
Rapeseed Meal ◽  
Concentrate Supplementation ◽  
Rumen Degradation ◽  
Grass Silage ◽  
Intestinal Digestion ◽  
Ruminal Degradation ◽  
The Individual

Four ruminally and duodenally cannulated non-lactating Finnish Ayrshire cows were used in a balanced 4 × 4 Latin square to study the effect of different concentrate supplements on the true partial and total-tract digestion (TTD) of grass silage, estimated by using the combined rumen-bag-intestinal-bag method. The cows were fed, at maintenance level, grass silage alone or supplemented with good-quality ground barley, ground barley and rapeseed meal, or ground barley and soybean meal. The determination of the proportion of grass silage degraded in the rumen (RD) was based on disappearance of feeds from nylon bags during the rumen incubation as a function of time, using the outflow rate of k = 0.0625. The intestinal digestion (ID) was estimated by the mobile-bag method with the residues that resisted degradation during the 16-h rumen incubation. Combination of these two was calculated to provide the TTD. Concentrate supplementation always caused a clear and consistent decline in rumen degradation and TTD of organic matter (OM), neutral detergent fibre (NDF) and Kjeldahl-N of grass silage but had no real influence on its ID. The type of concentrate, however, had only little effect. The average TTD of NDF was 16% lower than that of OM, but TTD of N was always very much higher than the respective value for OM. The results indicate that concentrate supplementation decreases the total-tract digestion of OM, cell walls and nitrogen of grass silage owing to impaired ruminal degradation. The combined bag method appears a convenient tool to provide digestion coefficients close to the true feed digestion of the individual feeds. Key words: Grass silage, nylon bag, mobile bag, combined bag, ruminal degradation, intestinal digestion, true digestion

scholarly journals Performance of growing dairy bulls offered diets based on silages made of whole-crop barley, whole-crop wheat, hairy vetch and grass

2008 ◽  
Vol 19 (2) ◽  
pp. 116 ◽  
Author(s):  
A. HUUSKONEN ◽  
E. JOKI-TOKOLA
Keyword(s):  
Energy Intake ◽  
A Priori ◽  
Carcass Characteristics ◽  
Rapeseed Meal ◽  
Feed Conversion ◽  
Hairy Vetch ◽  
Vicia Villosa ◽  
Concentrate Supplementation ◽  
Grass Silage ◽  
Dairy Bulls

The present experiment was conducted to study diet digestibility, feed intake, animal performance and carcass characteristics of growing dairy bulls offered diets based on (1) whole-crop barley, (2) a mixture of whole-crop barley and hairy vetch (Vicia villosa Roth.) or (3) a mixture of whole-crop wheat and hairy vetch relative to moderate digestible grass silage-based diet. The feeding experiment with 24 Finnish Ayrshire and 8 Holstein-Friesian bulls included 4 forage feeding treatments: (1) grass silage (G), (2) whole-crop barley and hairy vetch mixture silage (BHV), (3) whole-crop wheat and hairy vetch mixture silage (WHV) and (4) whole-crop barley silage (B). In all treatments animals were offered silage ad libitum. The amount of concentrate supplementation was 36 g (W0.75)-1 animal-1 day-1 for all treatments. The concentrate ration included rolled barley and rapeseed meal. Differences between the treatments were compared using an a priori test (Dunnett’s test) so that comparison of the diets was based on the G diet. The animals were fed the experimental diets from day 240 to finish at day 505 of age. During the experiment the average concentrate proportions of G, BHV, WHV and B diets were 437, 424, 426 and 423 g dry matter (DM) (kg DM)-1, respectively. There were no significant differences in silage DM intake or in the total DM intake (DMI) (kg DM d-1) between treatments. However, DMI kg-1 W0.75 tended to be 3.5% higher (p = 0.09) in the B diet than in the G diet. Due to increasing energy intake, the gain of the bulls was higher with the G diet than with the WHV diet (p < 0.05). BHV and B diets did not differ from the G diet in gain. Treatments had no significant effect on the dressing proportion or carcass conformation. The carcass fat score of WHV bulls was 29% lower (p < 0.05) than that of the G bulls, but BHV and B diets did not differ from the G diet in carcass fatness. The feed conversion rate (DM intake kg-1 carcass gain) of the bulls was better (p < 0.001) and protein conversion (g AAT kg-1 carcass gain) tended to be better (p = 0.07) with the G diet than with the WHV diet. BHV and B diets did not differ from the G diet in any feed conversion parameters. It can be concluded that replacing moderate digestible grass silage with whole-crop wheat and hairy vetch mixture silage decreased the carcass gain of growing dairy bulls due to lower energy intake and poorer feed conversion. Instead, replacing moderate digestible grass silage with whole-crop barley or with whole-crop barley and hairy vetch mixture silage resulted in no differences in the performance or carcass characteristics parameters of growing dairy bulls.;

Effect of leucaena supplementation level, and provision of urea, on utilisation by sheep of Rhodes grass hay

2001 ◽  
Vol 41 (2) ◽  
pp. 155 ◽  
Author(s):  
I. W. Karda ◽  
G. McL. Dryden
Keyword(s):  
Organic Matter ◽  
Rumen Fluid ◽  
Latin Square ◽  
Rhodes Grass ◽  
Microbial Nitrogen ◽  
Grass Hay ◽  
Digestible Organic Matter ◽  
Ruminal Degradation ◽  
Chloris Gayana ◽  
Dietary Treatments

Tarramba leucaena (Leucaena leucocephala cv. Tarramba) foliage had per kilogram dry matter, 169 g protein and 29.8 g condensed tannins. Its value as a supplement, given either with or without urea, to sheep given a low-quality Callide Rhodes grass (Chloris gayana cv. Callide) hay was studied. Six rumen fistulated sheep (mean s.d. liveweight, 34 1.4 kg) were used to compare 6 dietary treatments in an incomplete latin square design. Rhodes grass hay was given ad libitum either alone, or with urea 7 g/day (U), or with leucaena 150 g/day (L150), or leucaena with urea (L150U), or leucaena 300 g/day (L300), or leucaena with urea (L300U). Digestible organic matter intake was increased significantly by leucaena supplementation although digestibility of the whole diet did not alter. Rumen fluid ammonia-N was not altered by leucaena supplementation, but was increased by urea. This suggests that Tarramba foliage protein has some resistance to ruminal degradation. Liquid and solids passage rates were not affected by the treatments. Microbial nitrogen supply to the intestine (g/day), and the efficiency of microbial nitrogen synthesis (g/kg organic matter apparently digested in the rumen), were increased by leucaena supplementation (P<0.01). Microbial protein synthesis was the only response in which 300 g/day air-dry Tarramba foliage gave improved results over 150 g/day.

scholarly journals Effect of ammonia treatment of wheat straw with or without supplementation of potato protein on intake, digestion and kinetics of comminution, rumen degradation and passage in steers

1994 ◽  
Vol 72 (1) ◽  
pp. 147-165 ◽  
Author(s):  
S. J. Oosting ◽  
P. J. M. Vlemmix ◽  
J. Van Bruchem
Keyword(s):  
Organic Matter ◽  
Pore Size ◽  
Wheat Straw ◽  
Latin Square ◽  
Potato Protein ◽  
Faecal Excretion ◽  
Rumen Degradation ◽  
Fractional Rate ◽  
Beet Pulp ◽  
Kinetics Of

Untreated wheat straw (UWS) or ammoniated wheat straw without (AWS) or with (AWSP) a supplement of potato protein of a low rumen degradability was fed to three steers according to a 3 × 3 Latin square design. All rations were supplemented with sugar-beet pulp and minerals. Voluntary organic matter intake (OMI, g/kg0.75 per d) was 67.8, 76.0 and 80.1 for whole rations (51.1, 59.7 and 59.2 for straw) for UWS, AWS and AWSP respectively, which was significantly higher for AWS and AWSP than for UWS. Organic matter digestibility (OMD, g/kg) was 561, 596 and 625 for the respective rations UWS, AWS and AWSP, also significantly higher for AWS and AWSP than for UWS. The increased voluntary intake and digestion of ammoniated wheat-straw-based rations were associated with a significantly higher potentially degradable fraction (D) of neutral detergent fibre (NDF) in offered straw (556 and 661 g/kg for untreated and ammoniated wheat straw respectively) and in the rumen pool (469, 555 and 554 g/kg for UWS, AWS and AWSP respectively). Isolated small rumen particles (retained on sieves with a pore size < 1.25 and > 0.041 mm) had a significantly lower D of NDF (average 588 g/kg) than isolated large rumen particles (average 663 g/kg). Fractional rates of degradation of NDF did not differ significantly either between untreated and ammonia-treated wheat straw offered (2.9 and 2.6%/h respectively) or between rumen pools (1.8, 1.7 and 2.1 %/h for UWS, AWS and AWSP respectively). Rations based on ammoniated wheat straw had a significantly higher rumen NH3-N concentration than UWS. Although the rumen pool size of total contents differed significantly between treatments, those of dry and organic matter and of cell wall constituents were not significantly different. The proportion of rumen dry matter passing through a sieve with a pore size of 1.25 mm averaged 0.684 over rations (not significantly different between rations). Daily rumination (96 min) and eating (52 min) times/kg NDF ingested did not differ between rations. The rate of comminution of large particles estimated from the disappearance of indigestible NDF in large rumen particles from the rumen of animals without access to feed was 4.1, 6.3 and 7.1 %/h for UWS, AWS and AWSP respectively. These values were not significantly different. The fractional rate of passage estimated from the faecal excretion of Cr-NDF was 5.4, 6.1 and 6.3%/h for UWS, AWS and AWSP respectively (significantly higher for AWS and AWSP than for UWS) but the turnover rate of indigestible NDF did not differ between treatments.

Rumen degradation characteristics of ryegrass herbage and ryegrass silage are affected by interactions between stage of maturity and nitrogen fertilisation rate

2014 ◽  
Vol 54 (9) ◽  
pp. 1263 ◽  
Author(s):  
J. A. H. Heeren ◽  
S. C. Podesta ◽  
B. Hatew ◽  
G. Klop ◽  
H. van Laar ◽  
...  
Keyword(s):  
Organic Matter ◽  
Significant Interaction ◽  
Nutritional Value ◽  
Maturity Stage ◽  
N Content ◽  
Rumen Degradation ◽  
Grass Silage ◽  
Fertilisation Rate ◽  
Holstein Friesian ◽  
Friesian Cows

The objective of this experiment was to evaluate interaction effects between stage of maturity and nitrogen (N) fertilisation rate on rumen degradation characteristics determined with nylon bag incubations of ryegrass herbages and ryegrass silage. Grass herbage (n = 4) was cut after 3 or 5 weeks of regrowth and received a low (20 kg N/ha) or a high (90 kg N/ha) fertilisation rate. Grass silage (n = 6) received a low (65 kg N/ha) or high (150 kg N/ha) fertilisation rate and was harvested at early (~2000 kg DM/ha), mid (harvested 13 days later), or late (harvested 34 days later) maturity stage and ensiled in big bales. All grasses were incubated in the rumen of three lactating rumen-cannulated Holstein Friesian cows. Rumen degradation characteristics of organic matter (OM), N and neutral detergent fibre (NDF) and the extent of effective degradation (ED) were evaluated. In grass herbage, NDF content varied between 390 and 454 g/kg DM and N content between 12.1 and 25.8 g/kg DM. In grass silage, NDF content varied between 438 and 593 g/kg DM and N content between 13.4 and 34.8 g/kg DM. In general, rumen degradation of grass herbage and grass silage decreased with increased maturity, and increased with increased fertilisation rate. Significant interaction between maturity and fertilisation rate was observed for ED of OM, N and NDF, except for ED of N in grass herbage. These results indicate that the effect of the rate of N fertilisation on degradation of nutrients in the rumen of dairy cattle and on nutritional value depends on the grass maturity stage.

scholarly journals Response of silage intake and milk production to replacement of barley by barley fibre derived from integrated starch-ethanol process

1988 ◽  
Vol 60 (7) ◽  
pp. 711-721
Author(s):  
Pekka Huhtanen ◽  
Hannele Ala-Seppälä ◽  
Matti Näsi
Keyword(s):  
Milk Production ◽  
Crude Protein ◽  
Dry Matter ◽  
Latin Square ◽  
Rapeseed Meal ◽  
Neutral Detergent Fibre ◽  
Grass Silage ◽  
Mineral Mixture ◽  
Ad Libitum

A duplicated 4 x 4 Latin Square experiment was conducted to determine the effects of a gradually increased level of barley fibre in the diet on ad libitum grass silage intake and milk production. Barley fibre is a fibrous ethanol-starch by-product (120 g crude protein, 550 g neutral detergent fibre (NDF) and 120 g starch/kg dry matter (DM)). The four supplements, given at the rate of 6.5 kg/d on DM basis, were barley (B) and barley of which 333 g/kg (BF), 667 g/kg (FB) and 1000 g/kg (F) were replaced by barley fibre. In addition the cows were given 1 kg of rapeseed meal and 0.25 kg of mineral mixture. As the proportion of barley fibre in the diet increased there was a linear (P

scholarly journals Autumn-cut grass silage as roughage component in dairy cow rations. 2. Rumen degradation, fermentation and kinetics

1993 ◽  
Vol 41 (3) ◽  
pp. 221-234
Author(s):  
H. De Visser ◽  
H. Huisert ◽  
A. Klop ◽  
R.S. Ketelaar
Keyword(s):  
Rumen Fluid ◽  
Latin Square ◽  
Basal Diet ◽  
Rumen Fermentation ◽  
High Moisture ◽  
Rumen Degradation ◽  
Grass Silage ◽  
Branched Chain ◽  
Gluten Feed

In a 4 x 4 Latin square experiment the effects of DM content and/or the extent of fermentation in grass silages on the pattern of rumen fermentation and rumen kinetics were studied. In a separate study two cows were used to measure the rate of degradation using the dacron bag technique. Four rumen cannulated dairy cows were used to measure rumen fermentation pattern, rumen kinetics were measured in three of these animals. Basal diets (70% of total DM) consisted of maize silage, moist ensiled beet pulp, moist ensiled maize gluten feed, moist ensiled brewers' grains and a concentrate mixture. The remainder of the diet (30% of total DM) was wilted grass silage (WGS), high moisture grass silage with molasses (MGS), high moisture silage with formic acid (FGS) or wilted grass silage with additional water (WW). All diets were fed as complete feeds. pH of the rumen fluid was lower on the MGS and FGS diets. Concentrations of total VFA, acetic acid, ammonia and branched-chain fatty acids (BCFA) were highest on high moisture diets (MGS and FGS). Rates of clearance and digestion of the organic matter (OM) fractions were or showed tendencies towards being negatively influenced by both MGS and FGS, but remained unaffected by WGS and WW. Degradability of the grass silages was influenced by fermentation in the silo (lower digestible fractions and higher soluble fractions), as were rates of degradation (higher). Results of the degradability measured on the basal diet ingredients were in agreement with published literature and showed a strong correlation between OM digestibility in vitro and the undigestible fraction.

scholarly journals Effect of carbohydrates in grass silage-based diets on in sacco ruminal degradability of barley (Hordeum vulgare L. cv. Lomerit) grain ground to different particle sizes

2009 ◽  
Vol 54 (No. 6) ◽  
pp. 260-269
Author(s):  
B. Niwińska
Keyword(s):  
Particle Size ◽  
Hordeum Vulgare ◽  
Latin Square ◽  
Barley Grain ◽  
Particle Sizes ◽  
Rumen Degradation ◽  
Grass Silage ◽  
Degradation Rates ◽  
Size Interaction ◽  
In Sacco

The effect of carbohydrates included in grass silage-based diets on <I>in sacco</I> degradability of dry matter (DM), crude protein (CP), neutral detergent fibre (NDF) and cellulose (CE) of barley (<I>Hordeum vulgare</I> L. cv. Lomerit) grain ground to different particle sizes was studied using 3 ruminally cannulated cows. The measurements were carried out as a 3 × 2 Latin square design with treatments being carbohydrates in the concentrate portion of diets (starch in diet I vs. pectin in diet II vs. sucrose in diet III) and particle size of ground barley grain (1.0 vs. 5.7 mm). The diet synchronization index between N and energy supply during the day was an average of 0.8. The rumen degradability of barley grain compounds was influenced by the type of carbohydrates included in grass silage-based diets. Higher rumen degradation rates (<I>P</I> < 0.05) of barley CP, NDF and CE after 8 h of incubation and of CP and CE after 16 h of incubation were found in the rumen of cows receiving the diet containing sucrose compared with degradation rates found in the rumen of cows receiving the diet containing pectin or starch. The effective degradability of DM, CP, NDF and CE of barley grain in the rumen of cows receiving diets I, II and III was similar (77, 74, 39 and 41%, respectively). Replacing barley starch or beet pulp pectin with molasses sucrose in the grass silage-based diet resulted in the higher rate of barley CP, NDF and CE degradation after 8 h of incubation and of CP and CE after 16 h of incubation. It seems that the energy availability from carbohydrates affected the ruminal microbial activity as a consequence of the degradation rate of substrates. Higher (<I>P</I> < 0.01) rumen degradability of CP, NDF and CE after 2, 4, 8, 16 and 24 h of incubation was estimated for 1.0 mm particle size in comparison with that found for grain ground to 5.7 mm particle size, and the degradability of CP after 48 h of incubation was not different (<I>P</I> > 0.05). The degradability of barley grain CP, NDF and CE during incubation time was not influenced by the diet composition by particle size interaction (<I>P</I> > 0.05). The increase in barley grain particle size from 1.0 to 5.7 mm slowed down the rumen digestion of barley DM, CP, NDF and CE, probably due to restricted access to microbial digestion.

The effect of dried grass and cereal-based concentrate on the voluntary intake of unwilted grass silage

1977 ◽  
Vol 24 (2) ◽  
pp. 227-235 ◽  
Author(s):  
W. A. McIlmoyle ◽  
J. C. Murdoch
Keyword(s):  
Dry Matter ◽  
Latin Square ◽  
Live Weight ◽  
Crude Fibre ◽  
Concentrate Supplementation ◽  
Grass Silage ◽  
The Mean ◽  
Voluntary Intake ◽  
The Difference ◽  
Different Levels

SUMMARYTwo 7 × 7 Latin square experiments were carried out with British Friesian steers (mean initial live weight 360 kg). In Experiment 1 the effects of different levels of dried grass or concentrate on the intake of silage dry matter (DM) were compared, and in Experiment 2 the effect of a supplement containing various proportions of dried grass: concentrate on the intake of silage DM was examined. In both experiments the digestibility of the ration and the rate of passage of digesta were measured.In Experiment 1, supplementation depressed intake of silage DM, the difference being significant (P<0·05) at 5·0 and 7·5 kg/day, but there were no significant differences in silage intake between supplements given at the same level. Intake of silage was also significantly lower (P<0·05) in Experiment 2 when a supplement was offered, but differences between supplements were not significant. Total DM intake was increased significantly (P<00·5) by supplementation in both experiments. Supplementation increased energy digestibility but decreased crude fibre digestibility. In Experiment 1 nitrogen digestibility was significantly lower (P<0·05) with dried grass than with concentrate supplementation, but in Experiment 2 supplementation increased the digestibility of nitrogen. In both experiments the mean retention time was shorter, the time for the highest rate of excretion of stained particles was lower, and the maximum rate of excretion higher for dried grass than for silage.

Effect of degradability of protein supplements on voluntary intake and nitrogen retention in young cattle fed grass silage

1984 ◽  
Vol 39 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Margaret Gill ◽  
P. England
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Latin Square ◽  
Nitrogen Retention ◽  
Protein Supplementation ◽  
Protein Supplements ◽  
Grass Silage ◽  
Significant Difference ◽  
Digestible Organic Matter ◽  
Gross Energy

ABSTRACTGrass silage was offered ad libitum to twelve 4-month-old British Friesian steers in an incomplete Latin-square experiment. The silage was given either alone or supplemented isonitrogenously with 50 g fish meal or 63 g groundnut meal per kg silage dry matter.Both protein supplements significantly increased the intakes of dry matter and organic matter by an amount about equivalent to the (calculated) contribution of the supplement (P < 0·05); they also increased the intakes of digestible organic matter and nitrogen (P < 0·001) but with no significant difference between supplements. Protein supplementation had no effect on the intake of indigestible organic matter.The digestibility coefficients of dry matter and of organic matter were significantly increased by supplementation (P < 0·05) as were the digestibility coefficients of gross energy and of nitrogen (P < 0·001). Nitrogen retention was also increased from 1·4 to 8·2 g/day (P < 0·001), with no significant difference between supplements.

Comparison of energy and protein sources offered at low levels in grass-silage-based diets for dairy cows

1999 ◽  
Vol 68 (4) ◽  
pp. 789-799 ◽  
Author(s):  
R. J. Dewhurst ◽  
K. Aston ◽  
W. J. Fisher ◽  
R. T. Evans ◽  
M. S. Dhanoa ◽  
...  
Keyword(s):  
Energy Source ◽  
Dairy Cows ◽  
Milk Fat ◽  
Latin Square ◽  
Rapeseed Meal ◽  
Design Experiment ◽  
Significant Interaction Effect ◽  
Protein Sources ◽  
Grass Silage

AbstractFour dietary treatments were based on a flat-rate (5 kg/day) of concentrates with ad libitum grass silage. The concentrates were iso-energetic and iso-nitrogenous, based on either barley or unmolassed sugar-beet pulp and either extracted rapeseed meal (RSM) or a 1: 3 mixture of fish and soya-bean meals (F/S). These diets were offered to 61 multiparous Holstein-Friesian cows in a continuous design experiment from lactation weeks 4 to 22. Milk yields tended to he higher with RSM (25·1 v. 23·9 kg/day; s.e.d. = 0·64; P < 0·1), whilst milk fat (38·1 v. 40·0 g/kg; s.e.d. = 0·81) and milk protein (30·4 v. 31·3 g/kg; s.e.d. = 0·41) concentrations were significantly (P < 0·05) lower. There were no significant effects of treatments on the efficiency of conversion of food-nitrogen (N) to milk-N or on N-retention. A lower organic matter apparent digestibility (g/g) was found for RSM-based diets (0·738 v. 0·763; s.e.d. = 0·0096; P < 0·05). The diets were also offered to four fistulated dairy cows in a Latin-square-design experiment. Concentrate energy source had significant effects on rumen pH (P < 0·05) and ammonia-N concentration (P < 0·01), whilst protein sources had no effect; values were always in the optimal range (pH > 6 and ammonia-N > 50 mg/l). There was a significant interaction effect (P < 0·05) such that the N-degradability of the whole diet, estimated in vivo, was unaffected by energy source for RSM-based diets but highly dependent on energy source for FIS diets. Microbial protein yield was reduced on the RSM-based diets (179 v. 220 g/day; s.e.d. = 9·6; P < 0·001).

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