USE OF THE IN SITU TECHNIQUE AND PASSAGE RATE CONSTANTS IN PREDICTING VOLUNTARY INTAKE AND APPARENT DIGESTIBILITY OF FORAGES BY STEERS

1989 ◽  
Vol 69 (4) ◽  
pp. 973-987 ◽  
Author(s):  
MARINA A. G. VON KEYSERLINGK ◽  
G. W. MATHISON

The in situ bag technique was evaluated as a method of predicting the voluntary intake and digestibility of forages. Nine feedstuffs including three cereal straws, three alfalfa (Medicago sativa L.) and grass hay mixtures, two alfalfa-grass silages, and a prairie (Festuca hallii L.) grass hay were each fed to five steers (350 ± 49.5 kg) at ad libitum and approximately maintenance feeding levels. Hays and silages were also fed at near the twice maintenance feeding level. Voluntary forage dry matter (DM) intakes ranged from 1.00 to 2.85% of body weight. Apparent forage DM digestibilities ranged from 37 to 64%. At the lower feeding level fractional rates of particulate passage through the digestive tract were higher (P < 0.05) for 37% DM silage (0.037 h−1) than for two of the straw-based diets (0.020–0.023 h−1). Particulate passage rates were not (P > 0.05) affected by feeding level. Predictions from in situ results were most accurate when 24 and 36 h degradation values were used to estimate DM digestibility (R2 = 0.92) and intake (R2 = 0.86), respectively. The neutral detergent fiber content of forage was equally well correlated with DM digestibility (R2 = 0.86) and voluntary DM intake (R2 = 0.85). Inclusion of information on rates of passage in prediction equations did not (P > 0.05) improve accuracies of predictions over those obtained using in situ results or chemical feed components. Key words: Steers, in situ, voluntary intake, digestibility, passage rates, prediction

1998 ◽  
Vol 1998 ◽  
pp. 127-127
Author(s):  
B M L McLean ◽  
J J Hyslop ◽  
A C Longland ◽  
D Cuddeford

Cereal grains are often subjected to physical processing before being fed to equids. However, little information is available on how physical processing of cereals affects degradation dynamics in equids. This experiment examines the effect of two physical processing methods (micronisation and extrusion) on in situ degradation of barley in the caecum of poniesThree caecally fistulated mature Welsh-cross pony geldings (approx. LW 270kg) were offered ad libitum grass hay plus minerals. Incubation bags (monofilament polyester 6.5 x 20cm, 41μm pores, 16mg/cm2 sample size) containing either unprocessed barley (UB), micronised barley (MB) or extruded barley (EB) were incubated in the caecum for fixed times according to both a forward (0, 2, 4, 6, 12, 8, 24, 48h) and reverse (48, 24, 8, 4, 12, 6, 2, 0h) incubation sequence. For each feedstuff residues from each time were bulked within pony and across incubation sequence for subsequent analysis of dry matter (DM) and starch (STC). Degradation profiles were fitted to the DM and STC disappearance data according to Ørskov and McDonald (1979).


1998 ◽  
Vol 1998 ◽  
pp. 127-127 ◽  
Author(s):  
B M L McLean ◽  
J J Hyslop ◽  
A C Longland ◽  
D Cuddeford

Cereal grains are often subjected to physical processing before being fed to equids. However, little information is available on how physical processing of cereals affects degradation dynamics in equids. This experiment examines the effect of two physical processing methods (micronisation and extrusion) on in situ degradation of barley in the caecum of poniesThree caecally fistulated mature Welsh-cross pony geldings (approx. LW 270kg) were offered ad libitum grass hay plus minerals. Incubation bags (monofilament polyester 6.5 x 20cm, 41μm pores, 16mg/cm2 sample size) containing either unprocessed barley (UB), micronised barley (MB) or extruded barley (EB) were incubated in the caecum for fixed times according to both a forward (0, 2, 4, 6, 12, 8, 24, 48h) and reverse (48, 24, 8, 4, 12, 6, 2, 0h) incubation sequence. For each feedstuff residues from each time were bulked within pony and across incubation sequence for subsequent analysis of dry matter (DM) and starch (STC). Degradation profiles were fitted to the DM and STC disappearance data according to Ørskov and McDonald (1979).


1999 ◽  
Vol 1999 ◽  
pp. 134-134 ◽  
Author(s):  
B M L McLean ◽  
J J Hyslop ◽  
A C Longland ◽  
D Cuddeford ◽  
T Hollands

Maize and peas that have undergone physical processing are used routinely in cereal mixes for equines. However, little information is available on how physical processing of maize and peas affects degradation dynamics in equines. This experiment examines the effect of two physical processing methods (micronisation and extrusion) on in situ degradation of maize and peas in the caecum of poniesTwo caecally-fistulated mature Welsh-cross pony geldings (approx. LW 270kg) were offered ad libitum grass hay plus minerals. Incubation bags (monofilament polyester 6.5 x 20cm, 41? m pores, 16mg/cm2 sample size) containing either unprocessed maize (UM), micronised maize (MM), extruded maize (EM), unprocessed peas (UP), micronised peas (MP) or extruded peas (EP) were incubated in the caecum for fixed times according to both a forward (0, 2, 4, 6, 12, 8, 24, 48h) and reverse (48, 24, 8, 4, 12, 6, 2, 0h) incubation sequence. For each feedstuff residues from each time were bulked within pony and across incubation sequence for subsequent analysis of dry matter (DM) and starch (STC).


1996 ◽  
Vol 1996 ◽  
pp. 97-97
Author(s):  
J J Hyslop ◽  
G J Stefansdottir ◽  
D Cuddeford

Previous work as shown that incubation sequence has no effect on the degradation profile of feeds in the rumen of cattle and sheep. However, compared to the rumen the equine caecum is small, digesta passage rate through it is fast and total digesta volumes can vary widely. Consequently, this experiment examines the effect of bag incubation sequence on degradation co-efficients in situ in the equine caecum.Four caecally fistulated Welsh cross pony geldings (approx LW 250 kg) were offered ad libitum grass hay (DM: 938, CP: 70; NDF: 728) plus 30 g/h/d of a horse mineral supplement. Duplicate incubation bags (monofilament polyester, 6.5 x 20 cm, 41 um pores, 16 mg/cm2 sample size) containing a commercial horse concentrate (DM: 918, CP:151, NDF: 403) were incubated in the caecum for fixed times according to both a forward (0,3,5,16,8,24,48) or reverse (48,24,8,16,5,3,0) incubation sequence.


2021 ◽  
Vol 43 ◽  
pp. e53004
Author(s):  
Francyelle Ruana Faria da Silva ◽  
Ana Karina Dias Salman ◽  
Pedro Gomes da Cruz ◽  
Marlos Oliveira Porto ◽  
Jucilene Cavali ◽  
...  

To evaluate the bromatological composition and ruminal degradability of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) of Xaraés palisade grass (Urochloa brizantha ‘Xaraes’ syn Brachiaria brizantha) under grazing in integrated crop, livestock (ICL), and forest (ICLF) systems, we conducted an in situ degradability trial in randomized blocks with three non-lactating 3/4 Gyr × 1/4 Holstein cows, provided with ruminal cannula. The management of Xaraés palisade grass was similar in both systems, differing only regarding shading in the ICLF system provided by eucalyptus trees (average 65% crown cover). Grass samples were incubated for 0, 3, 6, 9, 12, 24, 36, 48, 72, and 96 hours. Considering the passage rate 2% h-1, the Xaraés palisade grass of ICL system had greater NDF effective degradability in relation to ICLF (46.38% vs 44.98%). However, the palisade grass CP potential degradability was greater in the ICLF than in the ICL system (68.92% vs. 65.40%). The presence of trees in the pasture has effect on nutritional traits of the Xaraés palisade grass, increasing its protein content and degradability and reducing its fiber degradability.


1969 ◽  
Vol 94 (1-2) ◽  
pp. 131-146
Author(s):  
Karla Tous-Rivera ◽  
Elide Valencia ◽  
Abner A. Rodríguez ◽  
Paul F. Randel ◽  
Adesogan Adegbola

Two experiments were conducted for determining effects of applying exogenous fibrolytic enzymes to guineagrass hay (GH) [Panicum maximum Jacq. (= Urochloa maxima (Jacq.) R. Webster)] upon chemical composition, voluntary intake (VI), digestibility of various chemical fractions, and apparent and true dry matter (DM) degradability. The enzymatic products used were PromoteNET and BiocellulaseA-20, derived from Trichoderma longibratum and Aspergillus reesei. These products contain mainly cellulase and xylanase. In the first experiment, nine mature Blackbelly x creole crossbred rams of 26.8 kg mean body weight (BW) were used in a 3 x 3 Latin Square design, with 18-d periods. Treatments consisted of untreated hay (control), hay treated with PromoteNET, and hay treated with BiocellulaseA-20, applied by spraying 24 h prior to feeding. Daily hay offerings were at 4% of BW on a dry matter (DM) basis. Enzyme treatment increased contents of DM and crude protein (CP) in the treated GH in comparison with those contents of the untreated hay. A tendency to reduce neutral detergent fiber (NDF), acid detergent fiber (ADF) and hemicellulose concentrations through the enzymatic treatments was also observed but was not significant (P > 0.05). The VI of DM of hays treated with BiocellulaseA-20 and PromoteNET (1,139 and 938 g DM/d, respectively) exceeded that of the control (921 g DM/d).The VI of CP, NDF and ADF were higher for hay treated with BiocellulaseA-20  than for GH untreated or treated with PromoteNET. Rams fed enzyme-treated hay had higher (P < 0.05) DM intake as a percentage of BW when compared with those fed untreated GH (4.06% for BiocellulaseA-20 0, 3.15% for PromoteNET, and 2.86% for the control). Apparent DM digestibility increased (P < 0.05) by 5.89 and 4.24 percentage units for BiocellulaseA-20 - and PromoteNET -treated hays in comparison with that of the control (50.12%). Significant increases over the control were observed in digestibility of three other fractions for GH treated with BiocellulaseA-20 and PromoteNET (CP, 3.23 and 3.18; NDF, 7.96 and 4.31; ADF, 7.69 and 7.9 percentage units). Digestibility of dry matter, CP and ADF did not differ (P > 0.05) between enzymatic treatments, whereas NDF digestibility was higher (P < 0.05) for GH treated with BiocellulaseA-20. In the second experiment, apparent dry matter degradability (IVDMDA) and true dry matter degradability (IVDMDT) were found to be higher (P < 0.05) for GH treated with both enzymes than for those of the control (IVDMDA by 2.75 percentage units for BiocellulaseA-20, 2.12 for PromoteNET; IVDMDT by 2.42 and 1.73 units, respectively). These results indicate that application of fibrolytic enzymatic complexes to low-quality tropical grass hay can improve the nutritional value substantially.


1996 ◽  
Vol 1996 ◽  
pp. 97-97
Author(s):  
J J Hyslop ◽  
G J Stefansdottir ◽  
D Cuddeford

Previous work as shown that incubation sequence has no effect on the degradation profile of feeds in the rumen of cattle and sheep. However, compared to the rumen the equine caecum is small, digesta passage rate through it is fast and total digesta volumes can vary widely. Consequently, this experiment examines the effect of bag incubation sequence on degradation co-efficients in situ in the equine caecum.Four caecally fistulated Welsh cross pony geldings (approx LW 250 kg) were offered ad libitum grass hay (DM: 938, CP: 70; NDF: 728) plus 30 g/h/d of a horse mineral supplement. Duplicate incubation bags (monofilament polyester, 6.5 x 20 cm, 41 um pores, 16 mg/cm2 sample size) containing a commercial horse concentrate (DM: 918, CP:151, NDF: 403) were incubated in the caecum for fixed times according to both a forward (0,3,5,16,8,24,48) or reverse (48,24,8,16,5,3,0) incubation sequence.


Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Kim Margarette C. Nogoy ◽  
Jia Yu ◽  
Young Gyu Song ◽  
Shida Li ◽  
Jong-Wook Chung ◽  
...  

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.


2017 ◽  
Vol 5 (2) ◽  
pp. 107
Author(s):  
Doohong Min ◽  
Iryna McDonald

The objective of this study was to evaluate the effect of planting alfalfa (Medicago sativa L.) with- and without cover crops on forage dry matter yield and nutritive values of alfalfa in Kansas, USA. In the first production year 2014, dry matter yield of mono-culture alfalfa appears to be compatible or even higher than planting with certain species of winter annual forages as a cover crop. Alfalfa planted with a combination of four different multi-species winter annual forages such as oats (Avena sativa L.), oilseed radish (Raphanus sativus L.), annual ryegrass (Lolium multiflorumL.), and turnip (Brassica rapa L.) had the highest dry matter yield of alfalfa in 2014.Dry matter yield of alfalfa in 2nd production year 2015 was similar to alfalfa + oats and alfalfa + oats + oilseed radish + annual ryegrass + turnip or significantly higher than alfalfa planted with annual ryegrass, oilseed radish, and turnip together. Based on two year of data in 2014 and 2015, the highest alfalfa yield occurred when alfalfa was planted with a combination of oats, oilseed radish, annual ryegrass, and turnip. The lowest dry matter yield of alfalfa occurred when alfalfa was planted with annual ryegrass.No significant differences in crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF) were found when comparing alfalfa monoculture with alfalfa + oats, alfalfa + oilseed radish, alfalfa + oats + oilseed radish + annual ryegrass + turnip, and alfalfa + turnip treatments. When considering both dry matter and nutritive values of alfalfa, planting alfalfa with cover crops appears to be compatible or better practice than planting alfalfa monoculture.


1970 ◽  
Vol 12 (1) ◽  
pp. 23-36 ◽  
Author(s):  
T. T. Treacher

SUMMARY1. Scottish Half-bred ewes carrying twin foetuses were fed individually to make live-weight gains in the last six weeks of pregnancy of (1) 20%, (2) 10% and (3) 0% of their live weight in week 14 of pregnancy. In lactation the ewes were fed ad libitum. The lambs were removed 12 to 16 hr after parturition and the ewes were machine-milked twice daily for the first six weeks of lactation.2. Total birth weights per ewe of twin lambs from the treatments were (1) 10·10 kg, (2) 9·44 kg and (3) 8·18 kg and differed significantly.3. The level and pattern of voluntary intake in lactation did not differ significantly between the treatments. Total dry-matter intakes in the six weeks of lactation were (1) 121·9 kg (2) 105·9 kg and (3) 109·5 kg.4. The pregnancy treatments affected the level of milk production and the shape of lactation curves. The total yields in the first six weeks of lactation were (1) 58·8 kg, (2) 43·5 kg and (3) 26·9 kg. Higher contents of fat and protein and the lower content of lactose in the milk from treatment-3 ewes on days 1 and 3 of lactation indicated a slower onset of lactation in these ewes. Between days 7 and 35 of lactation the contents of fat and SNF were lowest on treatment 3 but the differences were not significant.5. The live-weight changes in lactation, which were in inverse order to the gains in late pregnancy, were (1) 3·4 kg, (2) 5·5 kg and (3) 9·5 kg.


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