INFLUENCE OF AMMONIATION OF HIGH MOISTURE BARLEY ON ITS IN SITU RUMEN DEGRADATION AND INFLUENCE ON RUMEN FERMENTATION IN DAIRY COWS

1988 ◽  
Vol 68 (3) ◽  
pp. 839-851 ◽  
Author(s):  
P. H. ROBINSON ◽  
J. J. KENNELLY

High-moisture barley (HMB) was ammoniated at levels of 0, 0.65, 1.30, and 1.95% of dry matter (DM) and stored in 6-t lots in large plastic silage bags. In situ rumen degradation rate of DM in HMB decreased with increased level of ammoniation, nitrogen component degradation was little influenced, and neutral detergent residue degradation increased. Three dairy cows with large rumen cannulae were fed totally mixed diets twice daily in five experimental periods. Diets contained 490 g kg−1 oat silage, 385 g kg−1 HMB, and 125 g kg−1 of a protein-mineral-vitamin supplement. Measurements were made from weeks 18 to 33 of lactation to determine effects of ammoniation of HMB on levels and patterns of feed intake, as well as parameters of rumen fermentation. Cows tended to eat diets more rapidly as ammoniation level of substituted HMB increased, although total DM intake was not influenced. Rate of decline of rumen pH, and accumulation of butyrate, post-feeding was less pronounced as level of ammoniation of substituted HMB increased, supporting reduced rate of rumen degradation of HMB. However, total extent of change was greater, perhaps reflecting more rapid feed consumption post-feeding. Increased rumen acetate and butyrate concentrations as well as reduced concentration of 3 methyl butyrate suggests greater rumen fiber fermentation may have occurred as level of ammoniation of substituted HMB increased. Key words: Cows (dairy), ammoniation, high-moisture barley

1989 ◽  
Vol 69 (1) ◽  
pp. 195-203 ◽  
Author(s):  
P. H. ROBINSON ◽  
J. J. KENNELLY

Three dairy cows with large rumen cannulae were fed totally mixed diets twice daily. Diets contained 49% whole crop oat silage, 38.5% high-moisture barley (HMB), and 12.5% protein-mineral-vitamin supplement on a dry matter (DM) basis. HMB had been ammoniated at target levels of 0, 0.65, 1.30, and 1.95 g per 100 g HMB DM. Actual levels achieved were 0, 0.63, 1.30, and 1.95 g ammonia per 100 g. Apparent digestibility of organic matter (OM), neutral detergent (ND) fiber, starch, and N were not significantly influenced by level of ammoniation of HMB, although there was a trend for increased N digestion. Total rumen pool sizes of wet ingesta and DM were not influenced by ammoniation levels of HMB, although proportion of ND fiber in rumen OM declined as level of ammoniation of HMB increased. Milk yield and production of milk protein and lactose increased as level of ammoniation of HMB increased. Data are interpreted to suggest that rumen digestion of dietary nonfiber components declined as level of ammoniation of HMB increased, although digestion of ND fiber was not influenced. These results support the hypothesis that ammoniation of HMB shifted the site of digestion of a portion of the dietary nonfiber component from the rumen to the intestine without influencing rumen microbial pool sizes or degradative activity. Greater energetic efficiency of intestinal digestion of dietary nonfiber components, as level of ammoniation of HMB increased, may have contributed to the increased milk yield. Key words: Barley (high-moisture), ammoniation, dairy cows, digestion


1998 ◽  
Vol 1998 ◽  
pp. 63-63
Author(s):  
C. Rymer ◽  
D.I. Givens

The gas production (GP) technique has been developed to assess dynamics of ruminant digestion. Relationships have been observed between a feed's GP profile and in vivo parameters such as digestibility (Khazaal et al., 1993), feed intake and growth rate (Blümmel and Ørskov, 1993), and in situ degradability (Sileshi et al., 1997). However, there are few studies which relate GP data to the in vivo pattern of rumen fermentation (in terms of the rate of pH decline 2 h post-feeding and the mean rumen pH, concentration of total VFA and molar proportion of individual VFA). The object of this experiment was to determine whether such a relationship existed between a feed's GP profile and the pattern of rumen fermentation observed in animals fed that feed.


2020 ◽  
Vol 98 (2) ◽  
Author(s):  
Pedro Augusto Ribeiro Salvo ◽  
Viviane C Gritti ◽  
João Luiz Pratti Daniel ◽  
Leandro S Martins ◽  
Fernanda Lopes ◽  
...  

Abstract Exogenous fibrolytic enzymes (EFE) improve the energy availability of grains for nonruminant animals by reducing encapsulation of the endosperm nutrients within grain cell walls; however, these benefits are unknown in the treatment of corn-based silage for cattle. The objective of the present study was to evaluate the effects of adding EFE at ensiling on the nutritive value of high-moisture corn (HMC) and snaplage (SNAP) for finishing Nellore bulls. The EFE dose was 100 g/Mg fresh matter in both HMC and SNAP. Diets were 1) a SNAP + HMC control (without enzyme addition); 2) SNAP + HMC EFE (with enzymes); 3) a whole-plant corn silage (WPCS) + HMC control (without enzyme addition); and 4) WPCS + HMC EFE (with enzymes). In addition to the silages, the diets were also composed of soybean hulls, soybean meal, and mineral–vitamin supplement. The statistical design was a randomized complete block with a factorial arrangement of treatments, and the experiment lasted 122 d. For in situ and in vitro analyses, 2 cannulated dry cows were used. There was no interaction between the diets and EFE application (ADG, P = 0.92; DMI, P = 0.77; G:F, P = 0.70), and there was no difference between the SNAP and WPCS diets regarding the DMI (P = 0.53), ADG (P = 0.35), and feed efficiency (ADG:DMI, P = 0.83). Adding EFE to the HMC and SNAP at ensiling did not affect ADG but decreased DMI (P = 0.01), resulting in greater feed efficiency by 5.91% (P = 0.04) than that observed in animals fed diets without the addition of EFE. Addition of EFE to HMC resulted in reduced NDF content and increased in vitro and in situ DM digestibility compared with untreated HMC. No effects were found for the addition of EFE to SNAP. Fecal starch decreased with EFE application (P = 0.05). Therefore, the diet energy content (TDN, NEm, and NEg) calculated from animal performance increased (P = 0.01) with the addition of EFE to HMC. In conclusion, exchanging the NDF from WPCS with that from SNAP did not affect the performance of finishing cattle, whereas the addition of EFE to HMC at ensiling improved animal performance by increasing the energy availability of the grain.


1993 ◽  
Vol 41 (3) ◽  
pp. 221-234
Author(s):  
H. De Visser ◽  
H. Huisert ◽  
A. Klop ◽  
R.S. Ketelaar

In a 4 x 4 Latin square experiment the effects of DM content and/or the extent of fermentation in grass silages on the pattern of rumen fermentation and rumen kinetics were studied. In a separate study two cows were used to measure the rate of degradation using the dacron bag technique. Four rumen cannulated dairy cows were used to measure rumen fermentation pattern, rumen kinetics were measured in three of these animals. Basal diets (70% of total DM) consisted of maize silage, moist ensiled beet pulp, moist ensiled maize gluten feed, moist ensiled brewers' grains and a concentrate mixture. The remainder of the diet (30% of total DM) was wilted grass silage (WGS), high moisture grass silage with molasses (MGS), high moisture silage with formic acid (FGS) or wilted grass silage with additional water (WW). All diets were fed as complete feeds. pH of the rumen fluid was lower on the MGS and FGS diets. Concentrations of total VFA, acetic acid, ammonia and branched-chain fatty acids (BCFA) were highest on high moisture diets (MGS and FGS). Rates of clearance and digestion of the organic matter (OM) fractions were or showed tendencies towards being negatively influenced by both MGS and FGS, but remained unaffected by WGS and WW. Degradability of the grass silages was influenced by fermentation in the silo (lower digestible fractions and higher soluble fractions), as were rates of degradation (higher). Results of the degradability measured on the basal diet ingredients were in agreement with published literature and showed a strong correlation between OM digestibility in vitro and the undigestible fraction.


2000 ◽  
Vol 80 (1) ◽  
pp. 203-206 ◽  
Author(s):  
C. Bayourthe ◽  
R. Moncoulon ◽  
F. Enjalbert

Ruminal disappearance characteristics of OM, CP and starch relative to particle size of dehulled pea seeds were determined using the in sacco method. Fine grinding (≤502 µm) highly increased the rapidly CP and starch degradable fractions and the rate of degradation of these nutrients. Key words: Pea flour, particle size, rumen degradation, crude protein, starch


2011 ◽  
Vol 91 (1) ◽  
pp. 113-122 ◽  
Author(s):  
A. Nikkhah ◽  
C. J. Furedi ◽  
A. D. Kennedy ◽  
S. L. Scott ◽  
K. M. Wittenberg ◽  
...  

Nikkhah, A., Furedi, C. J., Kennedy, A. D., Scott, S. L., Wittenberg, K. M. Crow, G. H. and Plaizier, J. C. 2011. Morning vs. evening feed delivery for lactating dairy cows. Can. J. Anim. Sci. 91: 113–122. A study was conducted to investigate the effects of changing feed delivery time from morning (0900) to evening (2100) on feed intake, diurnal feed intake patterns, digestion, rumen fermentation, milk production and composition, as well as on several blood metabolites. Eight (four ruminally cannulated) lactating Holstein cows, consisting of four primiparous and four multiparous cows, were used in the study. The change in the time of feed delivery increased the proportion of feed consumed within 3 h of feed delivery in all cows (54.7 vs. 46.3±4.1%, means±SE, P=0.05), but increased daily dry matter intake only in primiparous cows (18.5 vs. 20.4±0.7 kg d−1, P=0.05). Average daily concentrations of blood glucose, lactate, urea and β-hydroxybutyrate (BHBA) as well as rumen pH and concentrations of volatile fatty acids (VFA) were not affected by feed delivery time. However, rumen pH was lower (P<0.05), at 5 and 6 h after feed delivery, while total rumen VFA and acetate were higher (P<0.05) only at 6 h after feed delivery in cows fed in the evening. Also, compared with feed delivery at 0900, feeding cows at 2100 resulted in lower (P<0.05) blood glucose and urea at 2 h after feed delivery, and increased BHBA (P<0.05) at 4 and 6 h after feed delivery. Cows fed at 2100 tended to produce more milk fat than those fed at 0900 (1.11 vs. 0.97±0.07 kg d−1, P=0.08). Milk yield and milk protein were not affected by the time of feed delivery. Changing the feed delivery time from morning to evening increased apparent total tract digestibility of dry matter, nitrogen, and neutral detergent fibre. The results of this study suggest that changing time of feed delivery from 0900 to 2100 increases feed consumption within 3 h after this delivery and rumen fermentation within 6 h after this delivery, changes diurnal patterns of glucose, urea and BHBA in peripheral blood, tends to increase milk fat, and increases nutrient digestibilities.


2021 ◽  
Vol 8 (12) ◽  
pp. 322
Author(s):  
Hathaichanok Insoongnern ◽  
Wuttikorn Srakaew ◽  
Tipwadee Prapaiwong ◽  
Napongphot Suphrap ◽  
Saisamorn Potirahong ◽  
...  

Ruminal pH is an important physiological parameter that regulates microbe activity; optimizing ruminal pH may improve rumen fermentation and milk production. The purpose of this experiment was to determine the effect of sodium bicarbonate (NaHCO3) or selenium (Se) in mineral salt block (MSB) supplementation on ruminal pH, rumen fermentation, milk yield and composition in Holstein Friesian crossbred dairy cows. Four crossbred dairy cows with an initial weight of 456 ± 6 kg in mid-lactation were assigned at random using a 4 × 4 Latin square design. The experiments were divided into four periods, each lasting 21 days. Each cow was fed a basal diet supplemented with a different type of mineral salt block: a control with no MSB supplementation, and MSB groups with MSB containing NaHCO3 (MSB-Na), MSB containing Se (MSB-Se), and conventional commercial MSB (MSB-Com). MSB-Na contained NaHCO3 (500 g/kg) to prevent acidosis, MSB-Se contained organic Se (15 mg/kg) as an antioxidant, and MSB-Com was a positive control mineral salt block. The results show that there was no significant difference in feed intake between treatments, but there was a significant difference in mineral salt intake between treatments (p < 0.05). Supplementing mineral blocks had no effect on nutrient intake or apparent digestibility (p > 0.05). Ruminal pH was not different between treatments at 0 and 1 h post-feeding, but at 2 and 4 h post-feeding, ruminal pH in cows fed MSB-Na and MSB-Se was significantly higher (p < 0.05) than it was in cows fed MSB-Com and the control. Total volatile fatty acid (VFA), acetic, propionic, butyric, and ammonia nitrogen and blood urea nitrogen were not influenced by mineral blocks supplementation. Milk yield, milk composition and energy-corrected milk (ECM) were not affected by supplementing mineral blocks. However, compared with the control, the somatic cell count (SCC) in the milk was reduced (p < 0.05) by supplementation with the mineral salt block. Based on the results of the experiments, it was concluded that MSB-Na or MSB-Se supplementation improved ruminal pH while having no effect on feed intake, rumen fermentation, milk yield, or composition, though it did reduce SCC in milk. However, additional research should be conducted to investigate the effect of MSB on rumen ecology and milk production in dairy cows fed a high-concentrate diet.


1995 ◽  
Vol 60 (3) ◽  
pp. 347-359 ◽  
Author(s):  
A. Cushnahan ◽  
C. S. Mayne ◽  
E. F. Unsworth

AbstractA study was carried out to examine the effects of ensiling and pattern of silage fermentation on nitrogen and energy utilization, rumen fermentation and rumen degradation characteristics of forage by lactating dairy cows. Six nonfistulated dairy cows and three rumen fistulated dairy cows were offered fresh grass (G) or grass silage produced from the same sward which had undergone either an extensive (E) or restricted (R) fermentation. Animals offered extensively fermented silage had liigher urinary nitrogen (N) outputs (expressed as a proportion of N intake) than those offered fresh grass (P < 0·05). Corresponding values for animals offered restricted fermented silage were intermediate between those offered grass and extensively fermented silage. Animals offered fresh grass and restricted fermented silage also had higher methane energy losses (P < 0·05), than those offered extensively fermented silage. The ensiling of grass had little effect on metabolizable energy (ME) intake or on the efficiency of utilization of ME for lactation (k1). Animals offered extensively fermented silage had higher proportions of propionate and lower proportions of acetate than those offered fresh grass or restricted fermented silage (P < 0·001). Ensiling resulted in an increase in the soluble (a) fraction and a reduction in the potentially degradable (b) fraction of N.


1998 ◽  
Vol 1998 ◽  
pp. 63-63 ◽  
Author(s):  
C. Rymer ◽  
D.I. Givens

The gas production (GP) technique has been developed to assess dynamics of ruminant digestion. Relationships have been observed between a feed's GP profile and in vivo parameters such as digestibility (Khazaal et al., 1993), feed intake and growth rate (Blümmel and Ørskov, 1993), and in situ degradability (Sileshi et al., 1997). However, there are few studies which relate GP data to the in vivo pattern of rumen fermentation (in terms of the rate of pH decline 2 h post-feeding and the mean rumen pH, concentration of total VFA and molar proportion of individual VFA). The object of this experiment was to determine whether such a relationship existed between a feed's GP profile and the pattern of rumen fermentation observed in animals fed that feed.


animal ◽  
2014 ◽  
Vol 8 (11) ◽  
pp. 1832-1838 ◽  
Author(s):  
M. Ali ◽  
G. van Duinkerken ◽  
J.W. Cone ◽  
A. Klop ◽  
M.C. Blok ◽  
...  

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