EFFECT OF TANNINS ON THE DIGESTIBILITY OF TWO ISOSYNTHETIC STRAINS OF BIRDSFOOT TREFOIL (Lotus corniculatus L.) USING IN VITRO AND IN SACCO TECHNIQUES

1988 ◽  
Vol 68 (3) ◽  
pp. 751-760 ◽  
Author(s):  
J. CHIQUETTE ◽  
K.-J. CHENG ◽  
J. W. COSTERTON ◽  
L. P. MILLIGAN
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
In Vitro Digestion ◽  
Lotus Corniculatus ◽  
Birdsfoot Trefoil ◽  
Dry Matter Loss ◽  
Forage Digestibility ◽  
High Concentrations ◽  
In Sacco

Two new isosynthetic strains of birdsfoot trefoil (Lotus corniculatus L.), containing low or high concentrations of tannin, were tested using in vitro and in sacco digestibility techniques. The high tannin (HT) strain contained an average of five times more tannin on a dry matter basis, than the low tannin (LT) strain. After 24-h in vitro digestion, by a rumen inoculum, there was less (P < 0.01) dry matter disappearance (45.7 vs. 54.4%) from the HT strain. Also, the development of gas pressure was lower (P < 0.01) and production of volatile fatty acids less (P < 0.05) with the HT strain than with the LT strain. Average dry matter loss from four replicated nylon bags was also lower (P < 0.01) for the HT strain than for the LT strain after 8, 12, and 24 h of incubation in rumen. These data indicate that a LT strain of birdsfoot trefoil might improve the forage digestibility. Key words: Birdsfoot trefoil, tannin, digestibility

NUTRITIVE VALUE OF SEDGE AND KENTUCKY BLUEGRASS HAYS GROWN IN ALBERTA

1977 ◽  
Vol 57 (1) ◽  
pp. 187-193 ◽  
Author(s):  
G. M. J. HORTON ◽  
L. W. McELROY
Keyword(s):  
Trichloroacetic Acid ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
In Vitro Digestion ◽  
Kentucky Bluegrass ◽  
Lake Bottom ◽  
Oat Straw

The nutritive values of sedge and Kentucky bluegrass hays grown in a partially drained lake bottom in central Alberta were assessed in the laboratory and compared with those of brome–fescue hay and oat straw. The extent and rate of in vitro digestion for each forage with rumen fluid from cows fed either sedge, Kentucky bluegrass or brome–fescue were measured using a two-stage technique. The forages were analyzed for proximate components and minerals, and the rumen fluid for trichloroacetic acid-insoluble nitrogen (TCA-N) and volatile fatty acids (VFA). The effects of sulphur and phosphorus supplementation on the in vitro digestion of purified cellulose in the presence of aqueous extracts of sedge, Kentucky bluegrass and brome–fescue were also determined. In vitro dry matter and cellulose digestibilities were highest for brome–fescue and lowest for oat straw; DMD values for Kentucky bluegrass were consistently higher (P > 0.05) than for sedge. Phosphorus supplementation did not enhance cellulose digestion with brome–fescue extract, but with sedge and Kentucky bluegrass extracts, the responses were significant. Ruminal TCA-N and VFA concentrations were highest after feeding brome–fescue, followed by Kentucky bluegrass and then sedge. These studies indicated that the nutritive value of brome–fescue hay was highest, followed by Kentucky bluegrass and sedge hays, and then oat straw.

scholarly journals Effects of high-amylase corn on performance and carcass quality of finishing beef heifers

2020 ◽  
Vol 98 (10) ◽  
Author(s):  
Lucas M Horton ◽  
Cadra L Van Bibber-Krueger ◽  
Hans C Müller ◽  
James S Drouillard
Keyword(s):  
Dry Matter ◽  
Block Design ◽  
Average Daily Gain ◽  
Crop Protection ◽  
In Vitro Digestion ◽  
Gas Production ◽  
Beef Heifers ◽  
Muscle Area ◽  
High Concentrations

Abstract Developed initially for use in fuel ethanol production, Enogen Feed Corn (EFC; Syngenta Crop Protection) is genetically modified to express high concentrations of α-amylase in the corn kernel. Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence. Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures. Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared. Grains were tempered with added moisture (0%, 3%, or 6%) prior to steam conditioning for 15, 30, or 45 min. No two- or three-way interactions were observed. Adding moisture improved starch availability (linear; P &lt; 0.01), and tended to improve ISDMD (linear, P = 0.06). Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P &lt; 0.01) compared with conditioning for 15 or 45 min. Starch availability, ISDMD, IVGP, acetate, propionate, valerate, and total VFA production increased with an increasing proportion of EFC (linear, P &lt; 0.01). Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively. Heifers were blocked by BW, stratified, and then randomly assigned to 28 dirt-surfaced pens (25 animals per pen). Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P &lt; 0.01), improving feed efficiency by 5% (P &lt; 0.01). Hot carcass weight was 6 kg greater for EFC cattle (P &lt;0.01) than CON. No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13). Cattle fed CON had greater marbling scores than EFC (P = 0.04), but this did not affect the USDA quality grade (P &gt; 0.33). Cattle fed EFC had 23% fewer abscessed livers than CON (P = 0.03). High-amylase corn may be used to improve microbial digestion, mill-throughput, and cattle performance, and it may mitigate liver abscesses.

In vitro selection for improved seedling vigour in birdsfoot trefoil (Lotus corniculatus L.)

1997 ◽  
Vol 77 (3) ◽  
pp. 385-390 ◽  
Author(s):  
N. L. McLean ◽  
J. Nowak
Keyword(s):  
Dry Matter ◽  
In Vitro Selection ◽  
Relative Effectiveness ◽  
Lotus Corniculatus ◽  
Dry Matter Production ◽  
Birdsfoot Trefoil ◽  
Seedling Vigour ◽  
Matter Production ◽  
C Cycle

Establishment of productive stands of birdsfoot trefoil (Lotus corniculatus L.) is often difficult due to poor seedling vigour. Objectives of this study were to evaluate the relative effectiveness of in vitro selection conditions for seedling vigour and to choose the best lines for further evaluation. Birdsfoot trefoil seedlings from cvs. Leo and Upstart were selected on the basis of in vitro rate of germination at 5, 12 and 20 °C and subsequent seedling growth at 20 °C. Two cycles of recurrent selection were carried out. Progeny from both cycles of selection were evaluated under controlled and field conditions. In general, selection temperatures did not significantly affect emergence, individual shoot dry matter production or total shoot dry matter production. Emergence and total shoot dry matter yield were higher for selections within cv. Upstart than for selections within cv. Leo. Seedling vigour of cycle-2 selections was not superior to cycle-1 selections. Failure to make gains during the second cycle of selection is believed to be due to seed sterilization procedures during cycle-2 selection. Germplasms chosen for further evaluation include the 12 °C and 20 °C cycle-1 selections and the 20 °C cycle-2 selection from cv. Upstart. Key words: Lotus corniculatus L., birdsfoot trefoil, seedling vigour, in vitro selection, temperature

USING A CELLULOLYTIC COMPLEX TO ESTIMATE IN VITRO DIGESTIBILITY OF BIRDSFOOT TREFOIL

1990 ◽  
Vol 70 (2) ◽  
pp. 487-493 ◽  
Author(s):  
D. K. DAVIS ◽  
R. L. McGRAW ◽  
D. A. SLEPER ◽  
P. R. BEUSELINCK
Keyword(s):  
Dry Matter ◽  
Lotus Corniculatus ◽  
Neutral Detergent Fiber ◽  
Birdsfoot Trefoil ◽  
In Vitro Digestibility ◽  
Detergent Solution ◽  
Two Stage ◽  
Fiber Digestion ◽  
Dry Matter Digestibility

Digesting forages with a prepared cellulase solution (PCS) has been shown to be a rapid method of obtaining dry matter digestibility (DMD) estimates in many species. This procedure has not been previously used to obtain DMD estimates of broadleaf birdsfoot trefoil (Lotus corniculatus L.). A broad based population consisting of 19 accessions, representing 11 countries of origin, three germplasm pools, and two domesticated cultivars of birdsfoot trefoil was evaluated. The PCS was produced from culturing Trichoderma reesei Simmons culture number QM9414. Fiber and PCS determinations were compared to an in vitro rumen fermentation (IVDMD) procedure. The fiber components measured were; neutral detergent fiber (NDF), acid detergent fiber (ADF), and hemicellulose (HEMI). The PCS procedures involved digestion using a PCS without any pre- or post-treatment (CS), acid-pepsin digestion followed by a single-stage digestion in PCS (APCS), acid detergent solution (ADS) followed by a two-stage digestion in PCS (ADSCS), and neutral detergent solution (NDS) followed by a two-stage digestion in PCS (NDSCS). All of the fiber and PCS procedures were significantly (P ≤ 0.001) correlated with IVDMD except HEMI. The CS procedure underestimated IVDMD by 199 g kg−1; however, it had the best regression coefficient (b = 0.99), had the second best correlation coefficient (r = 0.86), and required less time than the other PCS procedures. The APCS procedure provided the best estimate of IVDMD. It overestimated the IVDMD mean of birdsfoot trefoil by only 30 g kg−1 and was the most accurate (residual standard deviation (RSD) = 22 g kg−1) approach. The CS procedure is recommended when a ranking of samples is desired and actual values of DMD are not of primary interest.Key words: Fiber digestion, germplasm, in vitro dry matter digestibility, Lotus corniculatus L., Trichodenna reesei Simmons

scholarly journals 127 Porcine in vitro digestibility of whole stillage predigested with multi-enzyme during different time periods

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 49-50
Author(s):  
Kevin S Jerez Bogota ◽  
Tofuko A Woyengo
Keyword(s):  
Dry Matter ◽  
In Vitro Digestion ◽  
Enzyme Treatment ◽  
Optimal Time ◽  
In Vitro Digestibility ◽  
Percentage Points ◽  
Whole Stillage ◽  
Freeze Dried ◽  
Different Sources

Abstract A study was conducted to determine the effects of the period of predigesting whole stillage (WS; slurry material that is dried into DDGS) with multi-enzyme and composition of the multi-enzyme on porcine in vitro digestibility of dry matter (IVDDM) of the WS. Four samples of whole stillage from 4 different sources were freeze-dried and divided into 13 subsamples to give 52 sub-samples. Thirteen treatments were applied to the 48 sub-samples within source. The treatments were undigested WS (control); or pre-digested with 1 of 3 multi-enzymes (MTE1, MTE2, and MTE3) at 55 °C for 6, 12, 18 or 24 h in 3 × 4 factorial arrangement. The MTE1 contained xylanase, β-glucanase, cellulase, mannanase, protease, and amylase; MTE2 contained xylanase, α-galactosidase, and cellulase; and MTE3 contained xylanase, cellulase, β-glucanase, and mannanase. The 52 subsamples were subjected to porcine in vitro digestion. The IVDDM of untreated WS was 73.3%. The IVDDM increased (P&lt; 0.05) with an increase in the predigestion period. However, a rise in the predigestion period from 0 to 12 h resulted in greater (P&lt; 0.05) response in mean IVDDM than an increment in the predigestion period from 12 to 24 h (11 vs. 0.83 percentage points). Predigestion period and multi-enzyme type interacted on IVDDM such that the improvement in IVDDM between 0 and 12 hours of predigestion differed (P&lt; 0.05) among the 3 multi-enzyme types (13.3, 11.1, and 8.5 percentage points for MTE3, MTE2, and MTE1, respectively). The LS means by multi-enzyme treatment were modeled and resulted in unparallel curves (P&lt; 0.05). The estimated maximum response of IVDDM for MTE1, MTE2 and MTE 3 were 82.4%, 84.7% and 87.1% at 15.8, 13 and 13.1 hours, respectively. In conclusion, the optimal time of predigestion of WS with multi-enzymes (with regard to improvement in its IVDDM) was approximately 14 h.

scholarly journals Preserving Porphyra umbilicalis and Saccharina latissima as Silages for Ruminant Feeding

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1957
Author(s):  
Margarita Novoa-Garrido ◽  
Carlos Navarro Marcos ◽  
María Dolores Carro Travieso ◽  
Eduarda Molina Alcaide ◽  
Mogens Larsen ◽  
...  
Keyword(s):  
Dry Matter ◽  
Nutritional Value ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Saccharina Latissima ◽  
Porphyra Umbilicalis ◽  
In Vitro Gas Production ◽  
Ruminal Degradability ◽  
Kinetics Of

The study analyzed the characteristics, chemical composition, and in vitro gas production kinetics of Porphyra umbilicalis and Saccharina latissima silages. Each seaweed was ensiled in vacuum bags (three bags/silage) following a 2 × 3 factorial design, with two pre-treatments (unwilted or pre-wilted) and three silage types: unwashed seaweed ensiled without additive; seaweed washed and ensiled without additive; and seaweed washed and ensiled with 4 g of formic acid (FAC) per kg seaweed. Silages were kept for 3 months in darkness at 20 °C. Pre-wilting prevented (p < 0.001) effluent formation and reduced (p ≤ 0.038) the production of NH3-N and volatile fatty acids for both seaweeds. Both pre-wilting and washing increased (p < 0.05) the ruminal degradability of P. umbilicalis silages but not of S. latissima silages. The pH of the FAC-treated silages was below 4.0, but ranged from 4.54 to 6.23 in non FAC-treated silages. DL-lactate concentrations were low (≤23.0 g/kg dry matter) and acetate was the predominant fermentation product, indicating a non-lactic fermentation. The estimated ruminal degradability of the P. umbilicalis and S. latissima silages was as average, 59.9 and 86.1% of that for high-quality rye-grass silages, respectively, indicating a medium-low nutritional value of these seaweed silages for ruminants.

SOD-SEEDING BIRDSFOOT TREFOIL IN ESTABLISHED ORCHARDGRASS WITH OVERALL OR BANDED HERBICIDES

1983 ◽  
Vol 63 (1) ◽  
pp. 267-276 ◽  
Author(s):  
R. L. NICHOLS ◽  
R. A. PETERS
Keyword(s):  
Dry Matter ◽  
Dactylis Glomerata ◽  
Lotus Corniculatus ◽  
Birdsfoot Trefoil ◽  
No Tillage ◽  
Botanical Composition ◽  
Stand Establishment ◽  
Matter Production ◽  
Herbicide Treatments ◽  
Dactylis Glomerata L

Birdsfoot trefoil (Lotus corniculatus L. ’Viking’) was seeded directly into a predominantly orchardgrass (Dactylis glomerata L.) sward following overall or banded applications of paraquat (1,1′-dimethyl-4, 4′-bipyridinium ion, as dichloride salt) or glyphosate [N-(phosphonomethyl)glycine, as isopropylamine salt] to determine the degree of sward control necessary for stand establishment without tillage. Effects of the herbicide treatments and the seeding on botanical composition and dry matter production were determined by a vertical intercept technique and by hand separations of harvests into botanical components. Trefoil establishment was proportional to the degree of sward control during the seeding year. Little trefoil was established by direct seeding without herbicides. The banded herbicide treatments were less effective than overall herbicide applications for trefoil establishment. Trefoil yield obtained following overall application of paraquat was approximately 65% of that obtained following overall glyphosate treatment.Key words: Dactylis glomerata, glyphosate, Lotus corniculatus, herbicide, no-tillage, paraquat

scholarly journals The nutritional evaluation of forage-based mixed rations in New Zealand using an in vitro gas production technique. 1: analytical survey

2021 ◽  
pp. 1-6
Author(s):  
N.D. Meads ◽  
R. Tahmasbi ◽  
N. Jantasila
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Environmental Science ◽  
Ghg Emissions ◽  
Negative Relationship ◽  
Real Data ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Gas Production

Greenhouse gas (GHG) emissions from livestock are an important consideration in environmental science. Estimating GHG production can be problematic at a farm or animal level, and requires controlled conditions to produce real data. An in vitro gas production technique (IVGPT) was developed to evaluate forage-based total mixed rations in digestion kinetics and GHG production. Two hundred and sixty samples of complete mixed rations (MR), which included a pasture component used in commercial lactating dairy herds, were collected around NZ across three calendar years, 2017-2019. Twenty of the 260 samples were 100% total mixed rations (TMR) with no pasture content. The samples were submitted for proximate analysis as well as IVGPT to generate GHG production figures. The results showed an average total gas production (TGP) of 129.82 ml/g dry matter (DM), 78.6% true digestibility (TDMD), 125.06 mg/g DM microbial biomass (MB), 20.16 g CH4/kg DM, and 12.8 MJME/kg DM. The average nutrient composition was dry matter (DM) 31.55%, crude protein (CP) 21.85%, neutral detergent fibre (NDF) 44.35%, and starch 7.03%. The IVGPT CH4 production was negatively correlated to NDF (r=-0.312), ADF (r=-0.193), TGP (r=-0.216), and was positively correlated with TDMD (r=0.250), apparent digestibility (ADMD) (r=0.614), starch (r=0.117) and volatile fatty acids (r=0.538). The MR diet showed a strong positive relationship with ADMD digestibility (P=0.01) and a negative relationship with fibre content (NDF, P=0.01 and ADF, P=0.01). However, CH4 production reduced linearly with increasing TGP (P=0.01). The results indicated that a greater CH4 production may be related to higher digestibility of mixed ration.

Varietal differences in in vitro dry matter digestibility in Setaria, and the effects of site, age, and season

1972 ◽  
Vol 23 (6) ◽  
pp. 959 ◽  
Author(s):  
JB Hacker ◽  
DJ Minson
Keyword(s):  
Nitrogen Content ◽  
Dry Matter ◽  
Ash Content ◽  
High Potassium ◽  
Dry Matter Digestibility ◽  
Flowering Behaviour ◽  
High Concentrations ◽  
Ranking Order ◽  
Cutting Regimes

Six Setaria introductions were grown in small plots at three sites differing in soil type and location in south-eastern Queensland, and fertilizer and irrigation water were applied at similar rates. All plots of each introduction were genetically identical. Three different cutting regimes were imposed, such that at 12-week intervals during the 2 years of the experiment 4, 8, and 12-week regrowth was available for comparison of in vitro dry matter digestibility. There was an overall difference in digestibility between varieties of 6.6 percentage units, and the ranking order was usually the same for all sites, seasons, and ages of regrowth. Three of the introductions were consistently superior to cv. Nandi, the only commercial cultivar included in the present study. The two Setaria splendida introductions ranked second and sixth, although they were both higher in ash concentration than the other introductions, and this was associated with high potassium content. Differences between varieties in digestibility were not associated with flowering differences, despite large differences in flowering behaviour. The maximum mean difference between sites was 4.9 units and this was associated with differences in ash content. Difference in ash content, however, accounted for only 23% of the digestibility difference. The high ash content was associated with high concentrations of potassium. There was no association between nitrogen content and digestibility. The introduction lowest in digestibility had the highest nitrogen content. Digestibility was higher in winter than in summer at all sites, and the extent of this difference increased with age of regrowth. Higher digestibilities were obtained in younger regrowth.

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