INDUCTION OF NITRATE AND NITRITE METABOLISM IN BOVINE RUMEN FLUID AND THE TRANSFER OF THIS CAPACITY TO UNTREATED ANIMALS

1985 ◽  
Vol 65 (3) ◽  
pp. 647-652 ◽  
Author(s):  
K.-J. CHENG ◽  
R. C. PHILLIPPE ◽  
G. C. KOZUB ◽  
W. MAJAK ◽  
J. W. COSTERTON
Keyword(s):  
Body Weight ◽  
Key Words ◽  
Rumen Fluid ◽  
Microbial Activities ◽  
Rumen Microbes ◽  
Rumen Microflora ◽  
Nitrate And Nitrite ◽  
And Control ◽  
Key Words Nitrate

Nitrate, given intraruminally (0.1 g NO−3 per kg of body weight) to cattle, stimulated the capacity of the rumen microflora to degrade nitrite (NO−2) and nitrate in vitro. Threefold to fourfold increases in rates of NO−2 and NO−3 reduction were observed during NO−3 treatment. The treatment also appeared to stimulate nitropropanol degradation but this effect was less pronounced. The enhanced capacity of rumen microbes to degrade NO−2 and NO−3 was transferred to noninduced (untreated) animals housed in adjacent pens. The transfer of induced metabolism was not observed when induced animals were widely separated from the noninduced ones. This apparent transfer of microbial activities between adjacent animals may complicate studies in which treated and control animals are not separately housed. Key words: Nitrate, nitrite, nitropropanol, induction, degradation, cattle

Antioxidant potential of thyme extract: alleviation of N-nitrosodiethylamine-induced oxidative stress

2008 ◽  
Vol 27 (3) ◽  
pp. 215-221 ◽  
Author(s):  
P Rana ◽  
G Soni
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Test Group ◽  
Normal Diet ◽  
Lower Degree ◽  
Control Group ◽  
Albino Rats ◽  
Stress Induction ◽  
And Control

Protective role of thyme extract against N-nitrosodiethylamine (NDEA)-induced oxidative stress has been evaluated in albino rats. For this, one group of rats were fed diet supplemented with thyme extract (0.5%) and served as the test group, whereas animals of the other group fed on normal diet served as the control group. The rats were fed on respective diets for a period of 2 weeks after which stress was induced to half the animals of each group by i.p. administration of NDEA at 200 mg/kg body weight. Animals were killed 48 h post stress-induction period. Feed intake and body weight decreased significantly in both test and control groups, the effect being less in test group. Increase in osmotic fragility and in-vitro lipid peroxidation (LPO) on stress induction was of lower degree in the test group. NDEA toxicity was mainly reflected in liver as evidenced by increased activities of plasma aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase. The effect was of lower degree in test group as compared with that in the control group. Increase in urea levels observed following NDEA administration was also of lower degree in test groups. Blood glutathione (GSH) levels increased more so in test group compared with control group on stress induction. The activities of superoxide dismutase (SOD), peroxidase (Px), and catalase (CAT) activities decreased significantly on stress induction in erythrocytes. LPO increased in all the tissues through varying degree, and the increase was appreciably of lower degree in test group. The activity of SOD increased significantly in both test and control group on stress induction, whereas activities of Px and CAT decreased following NDEA treatment, and the effects were of lower degree in test group. Thus, supplementation of diet with thyme extract can improve antioxygenic potential and hence help to prevent oxidative stress.

The influence of adaptation of rumen microflora on in vitro digestion of different forages by sheep and red deer

2002 ◽  
Vol 80 (11) ◽  
pp. 1930-1937 ◽  
Author(s):  
Iain J Gordon ◽  
F Javier Pérez-Barbería ◽  
Paloma Cuartas
Keyword(s):  
Cervus Elaphus ◽  
Red Deer ◽  
In Vitro Digestion ◽  
Ovis Aries ◽  
In Vitro Digestibility ◽  
Rumen Microbes ◽  
Digestion Technique ◽  
Digestion Efficiency ◽  
Rumen Microflora

The rumen microflora ecosystem adapts to the diet consumed by the animal. We tested the extent to which this adaptation facilitates or retards the digestion of plant-based forages. Following adaptation of sheep (Ovis aries) and red deer (Cervus elaphus) to diets containing different mixtures of alfalfa, grass, and heather (a dwarf shrub), an in vitro digestion technique was used to compare the ability of the rumen microflora to digest the mixtures of substrates to which they were adapted with their ability to digest different mixtures of the same substrates. In vitro digestion of different substrates was slightly greater in rumen liquor derived from sheep than in that derived from red deer for each of the different substrates, but the effect was not significant. Digestibility in sheep was independent of how the feed was presented (diet of equal proportions of alfalfa, grass, and heather in each meal (D-EQ): mean in vitro digestibility = 37.3%; alfalfa, grass, and heather presented sequentially on different days (D-SEQ): mean in vitro digestibility = 37.7%, SE of differences = 1.30%, p > 0.05). However, in red deer there was a significant effect of method of diet presentation (D-EQ: mean in vitro digestibility = 36.9%; D-SEQ: mean in vitro digestibility = 34.2%, SE of differences = 1.30%, p < 0.05), digestibility being substantially lower for D-SEQ than for D-EQ. Overall, the results demonstrated that whilst there were no species-specific differences in overall digestion efficiency, dietary adaptation had an effect on substrate digestion efficiency, with rumen microbes adapted to high-quality diets digesting these more efficiently than low-quality diets.

Effects of heavy metals and other trace elements on the fermentative activity of the rumen microflora and growth of functionally important rumen bacteria

1978 ◽  
Vol 24 (3) ◽  
pp. 298-306 ◽  
Author(s):  
C. W. Forsberg
Keyword(s):  
Trace Elements ◽  
Rumen Fluid ◽  
Inhibitory Effect ◽  
Rumen Bacteria ◽  
Inhibitory Effects ◽  
Butyrivibrio Fibrisolvens ◽  
Fermentative Activity ◽  
Rumen Microflora ◽  
High Concentrations

The inhibitory effects of high concentrations of essential and non-essential trace elements were tested on the rumen microflora using the rate of fermentation in vitro as the assay. The elements (and the concentration causing 50% inhibition) in decreasing order of toxicity were Hg2+ (20 μg/ml), Cu2+ (21 μg/ml), Cr6+ (70 μg/ml), Se4+ (73 μg/ml), Ni2+ (160 μg/ml), Cd2+ (175 μg/ml), As3+ (304 μg/ml), and As5+ (1610 μg/ml). The elements tested that were either weak or non-inhibitory at concentrations greater than 400 μg/ml included Zn2+, Cr2+, Fe2+, Mn2+, Pb2+, and Co2+. Methylmercury was as inhibitory as mercuric chloride to the fermentation. When the inhibitory effect of Cd2+ was tested on separated bacterial and protozoal fractions, it was more inhibitory to the bacteria. The inhibitory effects of trace elements were also determined for a number of axenic cultures of rumen bacteria. The bacteria which most frequently exhibited the greatest sensitivity were Bacteroides succinogenes, Ruminococcus albus, Bacteroides amytophilus, and Eubacterium ruminantium. Those often exhibiting intermediate sensitivities included Butyrivibrio fibrisolvens, Selenomonas niminantium, and Megasphera elsdenii, while Streptococcus bovis was very refractory to all elements tested. Rumen fluid provided a modest protective effect for the bacteria.

Variation in number and proportion of rumen microbes examined both in vivo and in vitro using a simulation system

1998 ◽  
Vol 1998 ◽  
pp. 56-56
Author(s):  
Gisèle Gizzi ◽  
R. Zanchi ◽  
F. Sciaraffia
Keyword(s):  
Simulation System ◽  
Invasive Technique ◽  
Rumen Microbes ◽  
Rumen Metabolism ◽  
Rumen Microflora ◽  
Micro Organisms ◽  
Simulation Systems

The use of in vitro techniques is rapidly expanding because of an increasing need for routine and reproducible methods to obtain bioavailability data in addition to chemical ones. Furthermore in vivo methods are very expensive, an invasive technique for animals and may give non homogeneous results. In vitro systems could be divided in simulation systems (e.g. Rusitec, Czerkawski and Brekenridge, 1977) that attempt to closely reproduce the animal rumen ecosystem and are characterised by a higher level of complexity; and techniques that are mainly targeted to obtain biavailability data, without trying to mimic rumen metabolism (e.g. enzyme tests, Antoniewicz and Kosmala, 1997). As the fate of feedstufis in the rumen is mostly regulated by the micro-organisms, a rumen simulation system should primarily be characterised by a representative rumen microflora. The aim was to evaluate the possibility of maintaining a normal rumen microflora in an in vitro simulation system.

IN-VITRO FERMENTATION OF ALDITOLS BY RUMEN CONTENTS AND ABSORPTION OF ALDITOLS IN THE OVINE SMALL INTESTINE

1984 ◽  
Vol 64 (5) ◽  
pp. 41-42
Author(s):  
C. J. LISTER ◽  
R. R. SMITHARD
Keyword(s):  
Small Intestine ◽  
Key Words ◽  
Terminal Ileum ◽  
Rumen Fluid ◽  
Fluid Absorption ◽  
In Vitro Fermentation

In-vitro incubations with rumen fluid from four wethers showed that glucose, glucitol and mannitol were fermented more rapidly than arabinitol and xylitol and that arabinitol was fermented more rapidly than xylitol (P < 0.05) when the alditols or sugar were added singly. When arabinitol and xylitol or mannitol and xylitol were added as mixtures there was no effect on rates of individual alditol fermentation. Measurement of alditols reaching the duodenum and subsequently the terminal ileum of three sheep given an alditol mixture via the rumen showed that less than half the pentitols entering the small intestine were absorbed there. When higher levels of alditols entered the small intestine by infusion into the duodenum, absorption of pentitols was less efficient than that of the hexitols, mannitol and glucitol. Key words: Alditols, fermentation, rumen fluid, absorption

Metabolisable Energy Content of a Saponified Palm Fat Product for Ruminants

1988 ◽  
Vol 1988 ◽  
pp. 22-22
Author(s):  
D I Givens ◽  
Jeannie M Everington ◽  
N Shepperson
Keyword(s):  
Fatty Acids ◽  
Cell Walls ◽  
Energy Content ◽  
Rumen Fluid ◽  
Calcium Soap ◽  
Ruminant Diets ◽  
Soya Oil ◽  
Metabolisable Energy ◽  
Rumen Microflora

Many experiments have shown (see Armstrong and Ross, 1968) that the addition of fats to ruminant diets can lead to a reduction in the digestibility of forage cell walls due to adverse affects on the rumen microflora. Jenkins and Palmquist (1982) showed in vitro that the formation of insoluble calcium soaps in rumen fluid could prevent this problem. Additionally, Jenkins and Palmquist (1984) reported that the feeding of the preformed calcium soaps of tallow and soya oil fatty acids to dairy cows allowed normal rumen digestion of fibre whereas non-saponified tallow fatty acids caused a reduction in fibre digestion.The main aims of the present experiment were to examine a new calcium soap of palm fatty acids for its effects on whole tract digestibility of various feed fractions and to measure its effective metabolisable energy (ME) content at maintenance, the present United Kingdom standard.

scholarly journals MINIMIZE THE HYDROGENATION OF UNSATURATED FATTY ACID IN RUMEN WITH FORMALDEHYDE

2011 ◽  
Vol 11 (1) ◽  
pp. 43-47 ◽  
Author(s):  
Nafly C. Tiven ◽  
Lies Mira Yusiati ◽  
Rusman Rusman ◽  
Umar Santoso
Keyword(s):  
Fatty Acid ◽  
Unsaturated Fatty Acid ◽  
Rumen Fluid ◽  
In Vitro Fermentation ◽  
Rumen Microbes ◽  
The Difference ◽  
Female Sheep ◽  
Test Result ◽  
Range Test

This research aimed to know the ability of formaldehyde to protect unsaturated fatty acid of CPO on the hydrogenation process by rumen microbes. In this experiment, the in vitro fermentation of rumen fluid was carrying out. It was taken from the rumen-trocar of female sheep. The unsaturated fatty acid source was from CPO (Crude Palm Oil) which encapsulated by formaldehyde 37% within 0%, 1%, 2% and 3%. The data was analyzed by Completely Random Design with Duncan's New Multiple Range Test. The difference of means the treatments were tested by Duncan's New Multiple Range Test. Result showed that oleic and linoleic resulting from fermenting CPO protected by formaldehyde was increase if it was compared with the unprotected CPO. It can be concluded that encapsulated CPO with formaldehyde was able to prevent hydrogenating of unsaturated fatty acid, mainly oleic and linoleic.

scholarly journals The degradation and utilization of formaldehyde-treated urea by rumen microbes in vitro

1982 ◽  
Vol 54 (1) ◽  
pp. 15-24
Author(s):  
Jouko Setälä ◽  
Liisa Syrjälä-Qvist
Keyword(s):  
Organic Matter ◽  
Rumen Fluid ◽  
Total Yield ◽  
Ammonia Concentration ◽  
Tungstic Acid ◽  
Treatment Level ◽  
Microbial Protein ◽  
Buffer Solution ◽  
Rumen Microbes

Urea was treated with different levels of formaldehyde (HCHO). The HCHO percentages, on a weight basis, were 0(F0), 0.25 (F0.25) 0.50 (F0.50), 0.75 (F0.75), 1.0 (F1.0), 1.5 (F1.5), 2.0 (F2.0), 3.0 (F3.0) and 5.0(F5.0). Twenty milligrams of urea was incubated for 5 hours in 40 ml of sheep rumen fluid-buffer solution (1:1) together with 1.5 grams of substrate. The substrate consisted of vacuum-dried and milled feeds: barley (25 %), molassed beet pulp (25 %) and NaOH-treated straw (50 %). The feeds and urea were used in the same proportions as in the diet of the sheep which yielded the rumen fluid for incubation. Treatment with HCHO decreased hydrolysis of urea to ammonia. The ammonia concentration in contents offer mentors 2 hours after the start of incubation had a highly significant (P < 0.001) negative correlation (r = -0.976, n = 72) with the HCHO treatment level. Microbial protein synthesis was calculated from tungstic acid - sulphuric acid precipitation. Synthesis of protein, expressed as grams of nitrogen per 100 grams fermented organic matter was highest when F1.5-F3.0 urea was used. Treatment with more than 3 % of HCHO decreased the number of protozoa and the general activity of the microbes, thus decreasing fermentation of organic matter and lowering the yield of microbial protein. When F1.5 urea was used, the total yield (mg protein/hr) was significantly higher than with untreated urea, but the results obtained with F1.5 urea did not differ significantly from those with F0.75 or F2.0 urea.

Development of Protocol for Lyophilization of Goat Rumen Fluid

2021 ◽  
Author(s):  
A. Ruba Nanthini ◽  
C. Valli ◽  
L. Radhakrishnan ◽  
D. Balasubramanyam ◽  
A. Mangalagowri
Keyword(s):  
Rumen Fluid ◽  
Milk Powder ◽  
Skim Milk ◽  
Skim Milk Powder ◽  
Gas Production ◽  
Time Duration ◽  
Rumen Microbes ◽  
Alternate Source ◽  
Goat Rumen

Background: Rumen fluid has been used as microbial inoculum to treat indigestion in ruminant animals and to conduct in vitro rumen fermentation experiments. Lyophilization of the goat rumen fluid will provide continuous supply of rumen inoculums either for laboratory studies or for transfaunation in treating digestive disorders sequelae to high grain rations. However, no standard protocol is available for lyophilizing goat rumen fluid. Hence, this study was designed to develop a protocol to lyophilize goat rumen fluid as an alternate source for fresh goat rumen fluid. Methods: The study was conducted using 5 × 3 × 3 factorial design with four different cryoprotectants viz., 10% skim milk powder, 10% skim milk powder + 5% sodium glutamate, 5% glycerol, 5% DMSO and no cryoprotectant, at three pre freezing (2, 24 and 48 hours) and three freeze drying (8, 24 and 32 hours) time intervals to standardize protocol for lyophilizing goat rumen fluid. The viability of rumen microbes in the “lyophilized goat ruminal inoculum”, was determined via in vitro gas production study. Result: Pre freezing (-80°C deep freezer) duration of 48 hours with 32 hours of time duration in lyophilizer (-45°C) was ideal for lyophilizing goat rumen fluid with or without the addition of various cryoprotectants. Glycerol used at 5% as cryoprotectant resulted in significantly (P less than 0.05) highest gas production at all (12, 24 and 48) incubation hours studied indicating better viability of rumen microbes.

SEASONAL VARIATION OF IN VITRO DIGESTIBILITY IN THE KUDU, Tragelaphus strepsiceros

1984 ◽  
Vol 64 (5) ◽  
pp. 208-209 ◽  
Author(s):  
ELIZABETH A. BOOMKER
Keyword(s):  
Organic Matter ◽  
Seasonal Variation ◽  
Key Words ◽  
Rumen Fluid ◽  
In Vitro Digestibility ◽  
Tragelaphus Strepsiceros ◽  
High Cellulose

The seasonal variation and character of kudu rumen fluid inoculum was investigated. Three substrates used in in vitro fermentations yielded high cellulose digestibilities during the dry winter months. Organic matter digestibilities followed similar trends but did not reflect total rumen fermentative ability. Minimal hemicellulose digestion was observed. Key words: In vitro digestibility, cellulose, kudu

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